Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,460,841	G→A	G342D (GGC→GAC)	gspF →	Type II secretion system protein GspF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,460,841	0	G	A	100.0%	329.2 / NA	94	G342D (GGC→GAC)	gspF	Type II secretion system protein GspF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (49/45);  total (49/45)

CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTA  >  NC_000913/3460708‑3460974                                                                                                                                      |                                                                                                                                      cGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACAtt                                                                                                                                 >  1:1113301/1‑140 (MQ=255)  gCCGGCGTCGGCAAAAGGCGGCAGAGACCGTTCGCCGGGGTAACACCTTTCATTTTTCGCGGAAAAAAACCGCATTTTCCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTa                                                                                                                                <  2:64288/140‑1 (MQ=255)  cccAGCGTCGGGAAAAGGCGGCAGAGACCGTCCGCCGGGGTAACAGCATTCATCTTTCGCGGAAACAACCCGCATTTTCCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTa                                                                                                                                <  2:914683/139‑1 (MQ=255)        gTCTGGCAAATGGGGAAGAGAACGTCCGCCAGGTAAACACCATTCATTTTTCGCTGAAACAACCCAAAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCG‑AAAAAGCGGGCAGCTCGACACATTAATGGTCa                                                                                                                         <  2:638686/140‑1 (MQ=255)         tCTGACAAATGAGGCAGAGAACGTCCGCCAGGGAAACAGCTTTCATCTTTCGCTGAAAAAACCCGAATTTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCa                                                                                                                         <  2:364513/140‑1 (MQ=255)                 aTGGGGCAGAAACCTTCCGCCGGGGAAACAGCTTTCATTTTTCGCTGAAAAAACCCACATTTTCCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCa                                                                                                                 <  2:1591955/140‑1 (MQ=255)                 aTGCGGCAGAGAACGTTCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCa                                                                                                                 >  2:1155200/1‑140 (MQ=255)                 aTGCGGCAGAGAACGTTCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCa                                                                                                                 >  2:311732/1‑140 (MQ=255)                 aTGCGGAAGAGAACGTCCGCAGGGGAAACAGTATTCATTTTCCGCGGAAACAACCCGCAATTTCCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCa                                                                                                                 <  2:984650/140‑1 (MQ=255)                  tGCGGCAGAGAACGTTCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAg                                                                                                                >  1:1216039/1‑140 (MQ=255)                  ggCGGCAGAGACCGTTCGCCGGGGTAACAGCATCCTTCTTTCGCTGAAACAAACCGCATTTTCCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAg                                                                                                                <  2:1258295/139‑1 (MQ=255)                       cAGAGAACGTTCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAAc                                                                                                           >  1:396716/1‑140 (MQ=255)                        agagAACGTTCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAACCCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAAcc                                                                                                          <  1:549467/140‑1 (MQ=255)                            aaCGTTCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGga                                                                                                      >  2:1035263/1‑140 (MQ=255)                               gTTCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGac                                                                                                   >  2:288563/1‑140 (MQ=255)                                ttCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGaca                                                                                                  >  1:1647130/1‑140 (MQ=255)                                ttCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGaca                                                                                                  >  1:834471/1‑140 (MQ=255)                                ttCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGaca                                                                                                  >  1:1525972/1‑140 (MQ=255)                                    ccAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTcc                                                                                              >  1:44030/1‑140 (MQ=255)                                             cAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATc                                                                                     >  2:995278/1‑140 (MQ=255)                                                 tttCATCTTTCGCTGGAACAACCCGCATTTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGAt                                                                                 <  1:1531429/139‑1 (MQ=255)                                                  ttCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATc                                                                                >  2:1163420/1‑140 (MQ=255)                                                  ttCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATc                                                                                >  1:1061913/1‑140 (MQ=255)                                                  ttCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATc                                                                                >  1:211296/1‑140 (MQ=255)                                                  ttCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATc                                                                                >  2:85350/1‑140 (MQ=255)                                                  ttCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATc                                                                                >  1:346097/1‑140 (MQ=255)                                                      tCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCt                                                                            >  2:484002/1‑140 (MQ=255)                                                        tttCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTa                                                                          >  1:1559357/1‑140 (MQ=255)                                                         ttCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTaa                                                                         <  1:288563/140‑1 (MQ=255)                                                          tCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAAc                                                                        >  2:717058/1‑140 (MQ=255)                                                           ggCTGAAACAAACCGAAATTTCCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACg                                                                       <  2:1348123/139‑1 (MQ=255)                                                             cTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGct                                                                     >  2:450990/1‑140 (MQ=255)                                                             cTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGct                                                                     >  1:682179/1‑140 (MQ=255)                                                               ggAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGctct                                                                   <  1:311732/140‑1 (MQ=255)                                                                 aacaacCCGCATTTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTcc                                                                 <  1:689274/140‑1 (MQ=255)                                                                     aaCCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCt                                                             >  1:1654862/1‑140 (MQ=255)                                                                      ccccaaaTTTCCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCtt                                                            <  2:396716/135‑1 (MQ=255)                                                                      aCCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCtt                                                            >  1:634733/1‑140 (MQ=255)                                                                         gCATTTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGa                                                         <  1:647082/140‑1 (MQ=255)                                                                          cAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAg                                                        >  2:691076/1‑140 (MQ=255)                                                                             ttttCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCa                                                     <  2:1216039/140‑1 (MQ=255)                                                                              tttCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAg                                                    <  1:119871/140‑1 (MQ=255)                                                                               ttCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGc                                                   <  1:484002/140‑1 (MQ=255)                                                                                tCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCa                                                  <  1:73951/140‑1 (MQ=255)                                                                                tCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCa                                                  >  1:15084/1‑140 (MQ=255)                                                                                ccccGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCa                                                  <  1:566887/139‑1 (MQ=255)                                                                                ccccGCCGATGATGCTCTACATGGTGGCCTCGGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCa                                                  <  2:79999/139‑1 (MQ=255)                                                                                 cccGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCAc                                                 <  1:1235023/140‑1 (MQ=255)                                                                                  ccgccgATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACt                                                <  2:1559357/140‑1 (MQ=255)                                                                                  ccgccgATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACt                                                <  2:600500/140‑1 (MQ=255)                                                                                  ccgccgATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACt                                                <  1:1035263/140‑1 (MQ=255)                                                                                  ccgccgATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACt                                                >  1:1008231/1‑140 (MQ=255)                                                                                  ccgccgATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACt                                                <  1:1155200/140‑1 (MQ=255)                                                                                  ccgccgATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACt                                                <  1:871054/140‑1 (MQ=255)                                                                                    gccgATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCa                                              <  1:85350/140‑1 (MQ=255)                                                                                    gccgATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCAGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCa                                              <  2:762805/140‑1 (MQ=255)                                                                                        atgatgCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCattat                                          <  2:634733/140‑1 (MQ=255)                                                                                        atgatgCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCattat                                          <  2:979210/140‑1 (MQ=255)                                                                                                  aCATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCa                                >  2:814596/1‑140 (MQ=255)                                                                                                      ggtggCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGa                            >  1:1560729/1‑140 (MQ=255)                                                                                                      ggtggCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGa                            >  1:539295/1‑140 (MQ=255)                                                                                                       gtggCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGAt                           <  1:1147319/140‑1 (MQ=255)                                                                                                        tggCCTCTGGCGAAAAAAGCGGGTAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATc                          >  2:700754/1‑140 (MQ=255)                                                                                                        tggCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATc                          >  1:1402361/1‑140 (MQ=255)                                                                                                        tggCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATc                          >  1:231152/1‑140 (MQ=255)                                                                                                        tggCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATc                          >  2:915427/1‑140 (MQ=255)                                                                                                        tggCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATc                          <  2:682179/140‑1 (MQ=255)                                                                                                        tggCCTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATc                          >  1:684903/1‑140 (MQ=255)                                                                                                        tggCCTCTGGCG‑AAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCg                         >  2:1446481/1‑140 (MQ=255)                                                                                                           ccTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTc                       >  1:280942/1‑140 (MQ=255)                                                                                                           ccTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTc                       <  2:1188311/140‑1 (MQ=255)                                                                                                           ccTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTc                       <  1:639615/140‑1 (MQ=255)                                                                                                           ccTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTc                       <  1:1163420/140‑1 (MQ=255)                                                                                                           ccTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTc                       <  1:691076/140‑1 (MQ=255)                                                                                                           ccTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTc                       <  2:211296/140‑1 (MQ=255)                                                                                                           ccTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTc                       <  1:814596/140‑1 (MQ=255)                                                                                                           ccTCTGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTc                       <  2:1560729/140‑1 (MQ=255)                                                                                                           ccTCTGGCGAAAAAAGCGGGCAGCTCGACACAGTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTc                       >  2:1325211/1‑140 (MQ=255)                                                                                                             tctGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCt                     >  2:1127455/1‑140 (MQ=255)                                                                                                             tctGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCt                     >  2:1011904/1‑140 (MQ=255)                                                                                                             tctGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCt                     >  1:892776/1‑140 (MQ=255)                                                                                                             tctGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGACAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCt                     >  1:545247/1‑140 (MQ=255)                                                                                                             tctGGCGAAAAAAGCGGGCAGCTCGACACATTAAT‑GTCAGAGCCGCAGATAACCAGTAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTg                    >  2:39969/1‑140 (MQ=255)                                                                                                             tctGGCGAAAAAAGCAGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCt                     >  1:799392/1‑140 (MQ=255)                                                                                                              ctGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTg                    >  1:95473/1‑140 (MQ=255)                                                                                                               tGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGt                   >  1:44291/1‑140 (MQ=255)                                                                                                               tGGCGAAAAAAGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGt                   >  1:1293081/1‑140 (MQ=255)                                                                                                                ggCGAAAAAAGCGAGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGtt                  >  2:1042056/1‑140 (MQ=255)                                                                                                                    aaaaaaGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTAtt              >  2:555738/1‑140 (MQ=255)                                                                                                                        aaGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATtgtcg          <  2:346097/140‑1 (MQ=255)                                                                                                                        aaGCGGGCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATtgtcg          <  2:264170/140‑1 (MQ=255)                                                                                                                             ggcggcTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATtgtcgtgtcg     <  2:860741/140‑1 (MQ=255)                                                                                                                              gCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCgg    <  2:1008231/140‑1 (MQ=255)                                                                                                                              gCAGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCgg    <  2:1113301/140‑1 (MQ=255)                                                                                                                                aGCTCGACACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTa  <  2:684903/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                      CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGGTAACAGCATTCATCTTTCGCTGGAACAAACCGCAATTTTCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGGCACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTA  >  NC_000913/3460708‑3460974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑