BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,541,708	G→T	A434A (GCG→GCT)	feoB →	Fe(2(+)) transporter FeoB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,541,708	0	G	T	100.0%	389.4 / NA	112	A434A (GCG→GCT)	feoB	Fe(2(+)) transporter FeoB
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (57/55); total (57/55)

Only 100 of 116 total aligned reads displayed.

TTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGTTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCGCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGC  >  NC_000913/3541571‑3541843

ttGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGTTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGcgctcg                                                                                                                                       >  1:786854/1‑140 (MQ=255)

 tGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGTTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGt                                                                                                                                      <  1:245486/140‑1 (MQ=255)

 tGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGTTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGt                                                                                                                                      <  1:1074537/140‑1 (MQ=255)

       ggAAATCCTTTGTGCCGCTGATCGTCGGTTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCt                                                                                                                                >  1:1444403/1‑140 (MQ=255)

          aaTCCTTTGTGCCGCTGATCGTCGGTTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATc                                                                                                                             >  1:1667479/1‑140 (MQ=255)

             ccTTTGTGCCGCTGATCGTCGGTTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATcatc                                                                                                                          <  1:659767/140‑4 (MQ=255)

                   tgCCGCTGATCGTCGGTTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTa                                                                                                                    >  2:1579167/1‑140 (MQ=255)

                   tgCCGCTGATCGTCGGTTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTa                                                                                                                    >  2:908918/1‑140 (MQ=255)

                        cTGATCGTCGGTTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTcgc                                                                                                               >  2:179792/1‑140 (MQ=255)

                        cTGATCGTCGGTTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTcgc                                                                                                               >  1:1637898/1‑140 (MQ=255)

                        cTGATCGTCGGTTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTcgc                                                                                                               >  1:553567/1‑140 (MQ=255)

                        cTGATCGTCGGGTTCGGTTGTAACGTACCGACGGTGATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTcgc                                                                                                               >  1:128217/1‑140 (MQ=255)

                                  gtttcggttGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCcttc                                                                                                     >  2:751586/1‑140 (MQ=255)

                                   tttcggttGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCcttct                                                                                                    >  1:1215734/1‑140 (MQ=255)

                                   tttcggttGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCcttct                                                                                                    <  1:1131225/140‑1 (MQ=255)

                                   tttcggttGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCcttct                                                                                                    <  2:713076/140‑1 (MQ=255)

                                    ttcggttGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCcttctt                                                                                                   <  1:226329/140‑1 (MQ=255)

                                     tcggttGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTAATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGTCttcttc                                                                                                  >  1:967929/1‑140 (MQ=255)

                                        gttGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCggg                                                                                               <  2:1220199/140‑1 (MQ=255)

                                                   ccGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTgc                                                                                    >  1:1343180/1‑140 (MQ=255)

                                                   ccGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTgc                                                                                    >  2:1201113/1‑140 (MQ=255)

                                                   ccGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACAATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTgc                                                                                    >  1:1126117/1‑140 (MQ=255)

                                                      tCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCt                                                                                 >  2:673990/1‑140 (MQ=255)

                                                           aaTGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCgg                                                                            >  2:955829/1‑140 (MQ=255)

                                                              gggTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATTTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGtct                                                                         <  1:930859/140‑1 (MQ=255)

                                                                gTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCGCCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGtcttc                                                                       >  2:440505/1‑140 (MQ=255)

                                                                gTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGtcttc                                                                       >  2:1597230/1‑140 (MQ=255)

                                                                 tGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGtcttct                                                                      >  2:625591/1‑140 (MQ=255)

                                                                 tGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGtcttct                                                                      <  1:1147443/140‑1 (MQ=255)

                                                                 tGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGtcttct                                                                      >  1:9251/1‑140 (MQ=255)

                                                                 tGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGtcttct                                                                      >  2:729268/1‑140 (MQ=255)

                                                                 tGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGtcttct                                                                      >  1:550494/1‑140 (MQ=255)

                                                                  gCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTtctc                                                                     <  2:554219/140‑1 (MQ=255)

                                                                  gCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTtctc                                                                     <  1:865897/140‑1 (MQ=255)

                                                                  gCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTtctc                                                                     <  2:129529/140‑1 (MQ=255)

                                                                   cACGTACGCTTGATGCCCCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCCTCTCg                                                                    <  1:482908/140‑1 (MQ=255)

                                                                   cACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTtctcc                                                                    >  2:286048/1‑139 (MQ=255)

                                                                   cACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCg                                                                    <  1:179792/140‑1 (MQ=255)

                                                                   cACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCg                                                                    <  1:682648/140‑1 (MQ=255)

                                                                   cACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCg                                                                    >  1:998105/1‑140 (MQ=255)

                                                                   cACGTACGCTTCATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCg                                                                    <  1:475658/140‑1 (MQ=255)

                                                                    aCGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGc                                                                   >  2:1543023/1‑140 (MQ=255)

                                                                    aCGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGc                                                                   >  1:790918/1‑140 (MQ=255)

                                                                    aCGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTAGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGc                                                                   >  2:1489380/1‑140 (MQ=255)

                                                                     agTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCGATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCt                                                                  <  2:1114245/139‑1 (MQ=255)

                                                                       tACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGt                                                                <  2:553567/140‑1 (MQ=255)

                                                                       tACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGt                                                                <  1:751586/140‑1 (MQ=255)

                                                                          gCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGtata                                                             >  1:872946/1‑140 (MQ=255)

                                                                           cTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTatat                                                            <  2:1343180/140‑1 (MQ=255)

                                                                           cTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTatat                                                            >  1:68093/1‑140 (MQ=255)

                                                                             tGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGc                                                          <  1:1597230/140‑1 (MQ=255)

                                                                              gATGCACCGGGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCt                                                         <  2:1667479/140‑1 (MQ=255)

                                                                              gATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCt                                                         <  2:128217/140‑1 (MQ=255)

                                                                              gATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCt                                                         >  1:281006/1‑140 (MQ=255)

                                                                              gATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCt                                                         >  1:955570/1‑140 (MQ=255)

                                                                              gATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCt                                                         >  2:368228/1‑140 (MQ=255)

                                                                              gATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCt                                                         <  1:729268/140‑1 (MQ=255)

                                                                              gATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCt                                                         <  2:550494/140‑1 (MQ=255)

                                                                                tGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTgg                                                       >  2:1070623/1‑140 (MQ=255)

                                                                                 gCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTggg                                                      <  2:9251/140‑1 (MQ=255)

                                                                                    ccGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGTAt                                                   >  2:1050475/1‑140 (MQ=255)

                                                                                    ccGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGTAt                                                   <  1:1147890/140‑1 (MQ=255)

                                                                                    ccGCGTGAACGTCTGATGACCATCATGATGCCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGCATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGttt                                                   >  2:295545/1‑138 (MQ=255)

                                                                                      gcgTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGTATtg                                                 >  2:522433/1‑140 (MQ=255)

                                                                                         tGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGTATTGTGa                                              >  2:791099/1‑140 (MQ=255)

                                                                                              gTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGTATTGTGATGGCg                                         <  2:614787/140‑1 (MQ=255)

                                                                                              gTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGTATTGTGATGGCg                                         <  1:1070623/140‑1 (MQ=255)

                                                                                                 tgatgaCCATCATGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGTATTGTGATGGCGGTg                                      <  1:625591/140‑1 (MQ=255)

                                                                                                        catcatGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGTATTGTGATGGCGGTGCTGACTg                               >  1:1004593/1‑140 (MQ=255)

                                                                                                        catcatGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGTATTGTGATGGCGGTGCTGACTg                               >  1:1202879/1‑140 (MQ=255)

                                                                                                           catGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGTATTGTGATGGCGGTGCTGACTGGcc                            <  2:475672/140‑1 (MQ=255)

                                                                                                           catGATGGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGTATTGTGATGGCGGTGCTGACTGGcc                            <  1:746825/140‑1 (MQ=255)

                                                                                                            atgatgGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGTCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGTATTGTGATGGCGGTGCTGACTGGCCt                           <  2:967929/140‑1 (MQ=255)

                                                                                                              gatgGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGTATTGTGATGGCGGTGCTGACTGGCCTGa                         <  2:790918/140‑1 (MQ=255)

                                                                                                              gatgGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGTATTGTGATGGCGGTGCTGACTGGCCTGa                         <  1:673990/140‑1 (MQ=255)

                                                                                                              gatgGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGTATTGTGATGGCGGTGCTGACTGGCCTGa                         <  2:786854/140‑1 (MQ=255)

                                                                                                               atgGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGTATTGTGATGGCGGTGCTGACTGGCCTGAt                        >  2:896835/1‑140 (MQ=255)

                                                                                                                tgGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGTATTGTGATGGCGGTGCTGACTGGCCTGATg                       <  2:281006/140‑1 (MQ=255)

                                                                                                                tgGCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGTATTGTGATGGCGGTGCTGACTGGCCTGATg                       >  1:279566/1‑140 (MQ=255)

                                                                                                                 ggCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGc                      >  1:475314/1‑140 (MQ=255)

                                                                                                                  gCACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCt                     >  2:224532/1‑140 (MQ=255)

                                                                                                                   cACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTc                    <  1:685993/140‑1 (MQ=255)

                                                                                                                   cACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTc                    >  2:1166944/1‑140 (MQ=255)

                                                                                                                   cACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTc                    <  2:68093/140‑1 (MQ=255)

                                                                                                                   cACCGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCATTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTc                    <  2:1215734/140‑1 (MQ=255)

                                                                                                                     ccGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCaa                  <  1:522433/140‑1 (MQ=255)

                                                                                                                     ccGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCaa                  <  1:1579167/140‑1 (MQ=255)

                                                                                                                     ccGTTTATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCaa                  <  1:908918/140‑1 (MQ=255)

                                                                                                                        tttATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTa               >  2:533617/1‑140 (MQ=255)

                                                                                                                          tATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTaca             >  1:335899/1‑140 (MQ=255)

                                                                                                                          tATGTCCTGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTaca             <  2:872946/140‑1 (MQ=255)

                                                                                                                                 tGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACcatcat      >  1:374383/1‑140 (MQ=255)

                                                                                                                                 tGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACcatcat      >  1:738131/1‑140 (MQ=255)

                                                                                                                                 tGCGGCGCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACcatcat      >  2:38533/1‑140 (MQ=255)

                                                                                                                                  gcggcgCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCt                     >  1:353724/1‑124 (MQ=255)

                                                                                                                                  gcggcgCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCt                     <  2:353724/124‑1 (MQ=255)

                                                                                                                                  gcggcgCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATg     >  1:1331543/1‑140 (MQ=255)

                                                                                                                                   cggcgCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTa               >  1:1591836/1‑129 (MQ=255)

                                                                                                                                   cggcgCTCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTa               <  2:1591836/129‑1 (MQ=255)

                                                                                                                                     gcgctcGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATgcgc  <  2:1126117/140‑1 (MQ=255)

TTGCCGGGGAAATCCTTTGTGCCGCTGATCGTCGGTTTCGGTTGTAACGTACCGTCGGTAATGGGTGCACGTACGCTTGATGCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCGCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGC  >  NC_000913/3541571‑3541843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`