Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,525,774	A→C	I69M (ATT→ATG)	ansP ←	L‑asparagine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,525,774	0	A	C	100.0%	290.6 / NA	83	I69M (ATT→ATG)	ansP	L‑asparagine transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (43/40);  total (43/40)

AGTACATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAACTCAC‑GGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATAC  >  NC_000913/1525638‑1525906                                                                                                                                          |                                                                                                                                     aGTACATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAACTCAC‑GGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACAtt                                                                                                                                   <  1:1334447/140‑1 (MQ=255)   tACATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAACTCAC‑GGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATtaa                                                                                                                                 >  1:1365666/1‑140 (MQ=255)    aCATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAACTCAC‑GGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATtaaa                                                                                                                                >  1:587666/1‑140 (MQ=255)    aCATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAACTCAC‑GGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATtaaa                                                                                                                                >  2:368157/1‑140 (MQ=255)    aCATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAACTCAC‑GGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATtaaa                                                                                                                                >  1:612081/1‑140 (MQ=255)    aCATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAACTCAC‑GGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATtaaa                                                                                                                                >  2:802304/1‑140 (MQ=255)     cATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAACTCAC‑GGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATtaaat                                                                                                                               <  1:751840/140‑1 (MQ=255)       tCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAACTCAC‑GGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATtaaataa                                                                                                                             <  1:1635394/140‑1 (MQ=255)         cagccagcAACATAAGCGGCTTTCCCACCCAAAATCTCAA‑GGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAAc                                                                                                                           <  2:1545111/140‑1 (MQ=255)           gccagcAACATAAGCGGCTTTCTCACCCAAAAACTCAC‑GGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAAcca                                                                                                                         >  2:452026/1‑140 (MQ=255)           gccagcAACATAAGCGGCTTTCTCACCCAAAAACTCAC‑GGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAAcca                                                                                                                         >  1:1081282/1‑140 (MQ=255)           gccagcAACATAAGCGGCTTTCTCACCCAAAAACTCAC‑GGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAAcca                                                                                                                         >  2:1125557/1‑140 (MQ=255)            ccagcAACATAAGCGGCTTTCTCACCCAAAAACTCAC‑GGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAAccag                                                                                                                        >  1:936648/1‑140 (MQ=255)            ccagcAACATAAGCGGCTTTCTCACCCAAAAACTCAC‑GGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAAccag                                                                                                                        >  1:681518/1‑140 (MQ=255)               gcAACATAAGCGGCTTTCTCACCCAAAAACTCAC‑GGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAAccagtgc                                                                                                                     <  2:1047917/140‑1 (MQ=255)               gcAACATAAGCGGCTTTCTCACCCAAAAACTCAC‑GGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAAccagtgc                                                                                                                     <  2:428004/140‑1 (MQ=255)               gcAACATAAGCGGCTTTCTCACCCAAAAACTCAC‑GGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAAccagtgc                                                                                                                     <  1:1273620/140‑1 (MQ=255)               gcAACATAAGCGGCTTTCTCACCCAAAAACTCAC‑GGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAAccagtgc                                                                                                                     <  2:27271/140‑1 (MQ=255)                cAACATAAGCGGCTTTCTCACCCAAAAACTCAC‑GGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAAccagtgcc                                                                                                                    >  1:754766/1‑140 (MQ=255)                cAACATAAGCGGCTTTCTCACCCAAAAACTCAC‑GGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAAccagtgcc                                                                                                                    >  2:1634121/1‑140 (MQ=255)                        gCGGCTTTCTCACCCAAAAACTCAC‑GGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCggg                                                                                                            >  1:714760/1‑140 (MQ=255)                          ggCTTTC‑CCCCCAAAAACCCCCGGGGCAAAAAAAAAAAACCTGCCACTGGAAGGGCTGTGTAGCACCAGCTCCCCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGcc                                                                                                          <  2:1081282/140‑1 (MQ=255)                             tttCTCACCCAAAAACTCAC‑GGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCCCCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCg                                                                                                       <  2:797709/140‑1 (MQ=255)                             tttCTCACCCAAAAACTCAC‑GGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCg                                                                                                       >  1:559113/1‑140 (MQ=255)                             tttCTCACCCAAAAACTCAC‑GGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCg                                                                                                       >  2:1532584/1‑140 (MQ=255)                              ttCTCACCCAAAAACTCAC‑GGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGc                                                                                                      >  1:786449/1‑140 (MQ=255)                              ttCTCACCCAAAAACTCAC‑GGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGc                                                                                                      >  2:779912/1‑140 (MQ=255)                              ttCTCACCCAAAAACTCAC‑GGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGc                                                                                                      >  1:535146/1‑140 (MQ=255)                              ttCTCACCCAAAAACTCAC‑GGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGc                                                                                                      >  2:440994/1‑140 (MQ=255)                                  cACCCAAAAACTCAC‑GGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCAtt                                                                                                  <  1:609448/140‑1 (MQ=255)                                    cccAAAAACTCAC‑GGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTg                                                                                                <  1:452026/140‑1 (MQ=255)                                          accTCAC‑GGGCAAAAAAAAAAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCg                                                                                          <  2:612081/138‑1 (MQ=255)                                          aaCCCAG‑GGGAAAAAAAAAAAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCg                                                                                          <  2:728677/140‑1 (MQ=255)                                          aaCCCAC‑GGCAATAAAAAAAAAAACTGCCACTGGAAGGGCGGTGTAGCACCACCCCCCCCAATGCACGCAGAAAAAAAACCGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCg                                                                                          <  2:1476385/140‑1 (MQ=255)                                            ccccc‑GGGAATAAAAAAAAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCggg                                                                                        <  2:786449/136‑1 (MQ=255)                                            cTCAC‑GGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCggg                                                                                        <  1:703480/140‑1 (MQ=255)                                             tCAC‑GGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGc                                                                                       >  1:427917/1‑140 (MQ=255)                                             tCAC‑GGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGc                                                                                       >  1:1279134/1‑140 (MQ=255)                                               aC‑GGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTc                                                                                     >  1:820167/1‑140 (MQ=255)                                                       tAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCAcc                                                                              >  1:1106313/1‑140 (MQ=255)                                                        aaaaaaaaaaaCTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCAAATGCACCCAGAATAAAAACCGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCt                                                                             <  2:1106313/137‑1 (MQ=255)                                                        aaGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCt                                                                             <  1:1532584/140‑1 (MQ=255)                                                             aCAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTaaaaa                                                                        >  2:659403/1‑140 (MQ=255)                                                              cAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAAc                                                                       >  2:843515/1‑140 (MQ=255)                                                              cAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAAc                                                                       >  2:606368/1‑140 (MQ=255)                                                                 accTGCCACTGGAAGGGCGGTGTAGCCCCAGCTCACCCAATGCACGCAGAATAAAAACCGAAACCAAGCCCCACATTAAATAAACCAGGGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAg                                                                    <  1:383763/138‑1 (MQ=255)                                                                 aaCTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAg                                                                    <  1:440994/140‑1 (MQ=255)                                                                  aCTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGc                                                                   <  1:779912/140‑1 (MQ=255)                                                                     gCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCgg                                                                >  2:1664231/1‑140 (MQ=255)                                                                             aaGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATc                                                        >  2:755229/1‑140 (MQ=255)                                                                               gggCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATcgc                                                      <  2:427917/140‑1 (MQ=255)                                                                               gggCGGTGTAGCACCAGCCCCCCCAATGCACGCAAAATAAAAAACAAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATcgc                                                      <  2:1279134/140‑1 (MQ=255)                                                                               ggccGGGGTAGCCCCACCCCACCCATTGCACGAAAATTAAAAACCGAAAACAAGCCCCCCATGAAATAACCCATTGCGGGCCCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATcgc                                                      <  2:754766/137‑1 (MQ=255)                                                                                ggCGGTGTAGCCCCACCTCACCAAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAACCCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATcgcg                                                     <  1:1606418/140‑1 (MQ=255)                                                                                ggCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATcgcg                                                     <  2:198833/140‑1 (MQ=255)                                                                                ggCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATcgcg                                                     <  1:802304/140‑1 (MQ=255)                                                                                 gCGGTGTAGCCCCAGCCCCCCCAAGGCACGAAAAAAAAAAAACAAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCgcgc                                                    <  2:936648/140‑1 (MQ=255)                                                                                 gCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCgcgc                                                    >  2:1635348/1‑140 (MQ=255)                                                                                 gCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCgcgc                                                    >  1:1641948/1‑140 (MQ=255)                                                                                  cGGTGTAGCACCAGCTCCCCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGcgcc                                                   <  1:715040/140‑1 (MQ=255)                                                                                       tAGCACCAGCTCACCCAATGCACGCAGAAAAAAAAACAAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCaa                                              <  2:1114933/140‑1 (MQ=255)                                                                                            ccAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCg                                         <  1:491365/140‑1 (MQ=255)                                                                                            ccAGCTCACCAAATGCACGCAAAAAAAAAAACGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCg                                         <  1:368157/140‑1 (MQ=255)                                                                                               gCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGatc                                      >  1:944546/1‑140 (MQ=255)                                                                                               gCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGatc                                      >  2:347369/1‑140 (MQ=255)                                                                                               gCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGatc                                      >  1:717250/1‑140 (MQ=255)                                                                                               gCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGatc                                      >  1:456921/1‑140 (MQ=255)                                                                                               gCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGatc                                      >  1:1638961/1‑140 (MQ=255)                                                                                                   aCCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCt                                  >  1:1246012/1‑140 (MQ=255)                                                                                                    cccAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTg                                 >  1:1231269/1‑140 (MQ=255)                                                                                                    cccAATGCACGCAGAATAAAAAACGAAAACAAGCCACACATCAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTg                                 >  2:745640/1‑140 (MQ=255)                                                                                                         tGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCt                            >  2:1561974/1‑140 (MQ=255)                                                                                                         tGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCt                            <  2:681518/140‑1 (MQ=255)                                                                                                         tGCACGCAGAATAAAAAACGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCt                            <  1:606368/140‑1 (MQ=255)                                                                                                                 gAATAAAAAACGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTg                    >  2:226900/1‑140 (MQ=255)                                                                                                                    tAAAAAACGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGccc                 >  2:875490/1‑140 (MQ=255)                                                                                                                     aaaaaaCAAAAACAAGCCACACATAAAAAAACCAAGTGCCAGTGCGGGCCCCGCCATTTGTAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCa                <  2:1232324/140‑1 (MQ=255)                                                                                                                        aaaCGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCg             <  1:1631511/140‑1 (MQ=255)                                                                                                                         accGAAAACAACCCACACATAAAATAAACCTGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGc            <  2:944546/138‑1 (MQ=255)                                                                                                                          aCGAAAACAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCt           <  2:1641948/140‑1 (MQ=255)                                                                                                                           caaaaaCAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCtt          <  2:1438783/138‑1 (MQ=255)                                                                                                                             aaaaCAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTtg        <  1:1561974/140‑1 (MQ=255)                                                                                                                             aaaaCAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTtg        <  1:1539346/140‑1 (MQ=255)                                                                                                                             aaaaCAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTtg        <  1:1315117/140‑1 (MQ=255)                                                                                                                               aaCAAGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTtgtg      >  1:722810/1‑140 (MQ=255)                                                                                                                                  aacccACACATAAAATAAACCAGTGCCAGGGGGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATa   <  2:1179748/137‑1 (MQ=255)                                                                                                                                   aGCCACACATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATAc  >  2:192683/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                     AGTACATCCAGCCAGCAACATAAGCGGCTTTCTCACCCAAAAACTCAC‑GGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATAC  >  NC_000913/1525638‑1525906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑