Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,491,0980AC22.3% 267.0 / 15.9 121Y66S (TAC→TCC) cybBcytochrome b561
Reads supporting (aligned to +/- strand):  ref base A (59/35);  new base C (0/27);  total (59/62)
Fisher's exact test for biased strand distribution p-value = 4.80e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 128 total aligned reads displayed.

ATCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGATTGGTTTGGTGATGATGTATAACCGGGGCAAC  >  NC_000913/1490962‑1491231
                                                                                                                                        |                                                                                                                                     
aTCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCGCTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATAccc                                                                                                                                    >  1:1014930/1‑140 (MQ=255)
aTCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATAccc                                                                                                                                    >  1:343102/1‑140 (MQ=255)
aTCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATAccc                                                                                                                                    <  2:1067757/140‑1 (MQ=255)
       tgTATTGCGCAATGGAGTTTCGTTTTTTCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTTCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCccg                                                                                                                             <  2:343102/139‑1 (MQ=255)
       cGTATTGCGCATTGGATTTTCTTGGTTTCTTCCCTCGTAGGGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTTCTGATGGTCTTTCGTTTTTTGTTAAGGCTGAAATCCCCACCCccg                                                                                                                             <  2:418264/140‑1 (MQ=255)
       cGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCccg                                                                                                                             >  1:2136509/1‑140 (MQ=255)
       cGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCccg                                                                                                                             >  2:1714321/1‑140 (MQ=255)
       cGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCccg                                                                                                                             >  1:1144474/1‑140 (MQ=255)
       cGTATTGCGAAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCccg                                                                                                                             <  1:112726/140‑1 (MQ=255)
           ttGCGCAATGGAGTTTCGTGGTTTCTCCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATCCCCAACCCCGCCGa                                                                                                                         <  2:1343164/140‑1 (MQ=255)
            tGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGAt                                                                                                                        >  2:2071264/1‑140 (MQ=255)
            tGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGAt                                                                                                                        >  2:1523021/1‑140 (MQ=255)
            tGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGAt                                                                                                                        >  1:240904/1‑140 (MQ=255)
             gcgcAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATCCCCAACCCCGCCGAtt                                                                                                                       <  1:367414/140‑1 (MQ=255)
              cgcAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATta                                                                                                                      >  1:195416/1‑140 (MQ=255)
                 aaTGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATAc                                                                                                                   >  2:913382/1‑140 (MQ=255)
                 aaTGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATAc                                                                                                                   >  2:389475/1‑140 (MQ=255)
                  aTGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATAcc                                                                                                                  <  2:997416/140‑1 (MQ=255)
                  aTGGAGTTTCGTGGTTTCTCCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATAcc                                                                                                                  <  2:2289788/140‑1 (MQ=255)
                   tGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCt                                                                                                                 >  2:1991222/1‑140 (MQ=255)
                   tGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCt                                                                                                                 >  1:415135/1‑140 (MQ=255)
                       gTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGc                                                                                                             >  2:684115/1‑140 (MQ=255)
                         ttCGTGGTTTCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCa                                                                                                           <  1:709252/140‑1 (MQ=255)
                           cGTGGTTTCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCACCCCCGCCGATTATACCTAAGCCaaa                                                                                                         <  1:1714321/140‑1 (MQ=255)
                             tGGTTTCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAAc                                                                                                       >  1:666/1‑140 (MQ=255)
                              ggTTTCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCACCCCCGCCGATTATACCTAAGCCAAAAcc                                                                                                      <  2:1365413/140‑1 (MQ=255)
                                tttCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCga                                                                                                    >  2:1598783/1‑140 (MQ=255)
                                 ttcttcCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATCCCCACCCCCGCCGATTATACCTAAGCCAAAACCgat                                                                                                   <  2:2280544/140‑1 (MQ=255)
                                 ttcttcCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATCCCCACCCCCGCCGATTATACCTAAGCCAAAACCgat                                                                                                   <  2:2170057/140‑1 (MQ=255)
                                              gCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTg                                                                                      >  1:924031/1‑140 (MQ=255)
                                                gATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATCCCCACCCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGgc                                                                                    <  2:1625084/140‑1 (MQ=255)
                                                 aTCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGgcg                                                                                   >  1:2099007/1‑140 (MQ=255)
                                                       cACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTg                                                                             <  2:210001/140‑1 (MQ=255)
                                                         cTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAGAACCGATGATGACGGGACTGGCGCATTTggg                                                                           >  2:2346184/1‑140 (MQ=255)
                                                           catcaACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGAc                                                                         >  2:2157967/1‑140 (MQ=255)
                                                            atcaACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACa                                                                        >  2:2291159/1‑140 (MQ=255)
                                                            atcaACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACa                                                                        >  1:906763/1‑140 (MQ=255)
                                                             tcaACAGGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGCCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACAt                                                                       <  1:1242117/140‑1 (MQ=255)
                                                               aaCATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACAttt                                                                     <  1:1819121/140‑1 (MQ=255)
                                                                aCATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTg                                                                    >  1:2211196/1‑140 (MQ=255)
                                                                aCATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTg                                                                    <  1:75385/140‑1 (MQ=255)
                                                                  ttaaTTAATGTTTCCTGTGGCATCTCAATTCTCTTGCTGTTGTTTGTCGGTTTTTTGTTAAGGCTGAAATCCCCACCCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGt                                                                  <  2:543235/137‑1 (MQ=255)
                                                                  aTGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATCCCCACCCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGt                                                                  <  2:549390/140‑1 (MQ=255)
                                                                  aTGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCACCCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGt                                                                  <  2:1446871/140‑1 (MQ=255)
                                                                  aTGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGt                                                                  <  1:2071264/140‑1 (MQ=255)
                                                                   tGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTg                                                                 >  2:104947/1‑140 (MQ=255)
                                                                    gATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGa                                                                >  1:1759136/1‑140 (MQ=255)
                                                                      ttCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTATGGCTGAAATACCCAACCCTGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGAtt                                                              >  1:61888/1‑140 (MQ=255)
                                                                       tCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATCCCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGAttt                                                             <  1:482826/140‑1 (MQ=255)
                                                                       tCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATCCCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGAttt                                                             <  2:195416/140‑1 (MQ=255)
                                                                         aTGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTAt                                                           <  2:666/140‑1 (MQ=255)
                                                                           gTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATCCCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACa                                                                        <  1:2116677/125‑1 (MQ=255)
                                                                           gTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACa                                                                        >  2:2116677/1‑125 (MQ=255)
                                                                           gTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTAtct                                                         >  1:1302293/1‑140 (MQ=255)
                                                                            tttCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCACCCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTAtctc                                                        <  1:735774/140‑1 (MQ=255)
                                                                            tttCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTAtctc                                                        <  2:2136509/140‑1 (MQ=255)
                                                                            tttCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTAtctc                                                        >  1:1558548/1‑140 (MQ=255)
                                                                               ccTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTg                                                     >  1:2039540/1‑140 (MQ=255)
                                                                                cTGTGGCATCTCAATTCTCGTGCCGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGt                                                    >  2:1618749/1‑140 (MQ=255)
                                                                                   tgGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTa                                                 >  1:847030/1‑140 (MQ=255)
                                                                                     gCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTAtt                                               >  1:1832387/1‑140 (MQ=255)
                                                                                     gCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTAtt                                               >  2:1485104/1‑140 (MQ=255)
                                                                                        tctcAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATCCCCACCCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTgcg                                            <  2:2328605/140‑1 (MQ=255)
                                                                                        tctcAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATCCCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTgcg                                            <  1:33955/140‑1 (MQ=255)
                                                                                        tctcAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCACCCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTgcg                                            <  2:2211196/140‑1 (MQ=255)
                                                                                        tctcAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTgcg                                            >  1:1616409/1‑140 (MQ=255)
                                                                                        tctcAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTgcg                                            >  1:2298669/1‑140 (MQ=255)
                                                                                        tctcAATTCCCGTGCTGATGGTCGTTCGTTTTTTGTTAAGGCTGAAATCCCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTAATgcg                                            <  1:578408/140‑1 (MQ=255)
                                                                                         ctcAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTgcgc                                           >  1:2274444/1‑140 (MQ=255)
                                                                                         ctcAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACC‑‑‑GATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGc                                        <  1:708422/140‑1 (MQ=255)
                                                                                             aTTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGcc                                       >  1:52942/1‑140 (MQ=255)
                                                                                                ctcGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGt                                    >  1:815242/1‑140 (MQ=255)
                                                                                                ctcGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGt                                    >  2:514234/1‑140 (MQ=255)
                                                                                                  cGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGa                                  <  1:1523021/140‑1 (MQ=255)
                                                                                                   ttGCTGTTGGCGGTTCGTCTTTTGTTAAGGCTGAAATCCCCACCCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGAt                                 <  2:847030/139‑1 (MQ=255)
                                                                                                   gTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGAt                                 >  2:820800/1‑140 (MQ=255)
                                                                                                   gTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGAt                                 <  1:628384/140‑1 (MQ=255)
                                                                                                   gTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGAt                                 >  1:1748791/1‑140 (MQ=255)
                                                                                                   gTGCTGAGGGTCGTTCGTCTTTTGTTAGGGCTGAAATCCCCACCCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGAt                                 <  2:488063/140‑1 (MQ=255)
                                                                                                       tGATGGTCTTTCGTTTTTTGTAAAGGCTGAAATCCCCACCCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGAttggt                             <  2:2298669/140‑1 (MQ=255)
                                                                                                        gATGGTCGTTCGTTTTTTGTTAAGGCTGAAATCCCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGAttggtt                            <  1:229259/140‑1 (MQ=255)
                                                                                                         aTGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCACCCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGAttggttt                           <  1:104947/140‑1 (MQ=255)
                                                                                                         aTGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGAttggttt                           <  1:2292074/140‑1 (MQ=255)
                                                                                                            gTCGTTCGTCTTTTGTTAAGGCTGAAATACCCACCCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGAttggtttggt                        <  1:1267953/140‑1 (MQ=255)
                                                                                                              cgttcgttTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGATTGGTTTGGtga                      <  1:8517/140‑1 (MQ=255)
                                                                                                              cgttcgtCTTTTGTTAAGGCTGAAATCCCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGATTGGTTTGGtga                      <  2:1558548/140‑1 (MQ=255)
                                                                                                              cgttcgtCTTTTGTTAAGGCTGAAATCCCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGATTGGTTTGGtga                      <  2:924031/140‑1 (MQ=255)
                                                                                                              cgttcgtCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGATTGGTTTGGtga                      <  1:820800/140‑1 (MQ=255)
                                                                                                              cgttcgtCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGATTGGTTTGGtga                      <  2:750964/140‑1 (MQ=255)
                                                                                                                   gtCTTTTGTTAAGGCTGAAATCCCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCC‑GTTTATTGCGCTGCCAGTGAt                                 <  2:677904/123‑1 (MQ=255)
                                                                                                                   gtCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGa                                  <  2:966278/123‑1 (MQ=255)
                                                                                                                   gtCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGa                                  >  1:966278/1‑123 (MQ=255)
                                                                                                                   gtCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGATTGGTTTGGTGatgatg                 >  2:1451694/1‑140 (MQ=255)
                                                                                                                    ttttttGTAAAGGCTGAAATCCCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGATTGGTTTGGTGATGATGt                <  2:240904/138‑1 (MQ=255)
                                                                                                                    tCTTTTGTTAAGGCTAAAATCCCCACCCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGATTGGTTTGGTGATGATGt                <  2:1248907/140‑1 (MQ=255)
                                                                                                                      ttttGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGATTGGTTTGGTGATGATGtat              >  1:1248907/1‑140 (MQ=255)
                                                                                                                         tGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGATTGGTTTGGTGATGATGTATAAc           >  1:1435697/1‑140 (MQ=255)
                                                                                                                             aaGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGATTGGTTTGGTGATGATGTATAACCggg       <  1:2058691/140‑1 (MQ=255)
                                                                                                                                 cTGAAATACCCACCCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGATTGGTTTGGTGATGATGTATAACCGGGGCaa   <  2:1865115/140‑1 (MQ=255)
                                                                                                                                  tGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGATTGGTTTGGTGATGATGTATAACCgggttgcc  <  2:1832387/140‑6 (MQ=255)
                                                                                                                                        |                                                                                                                                     
ATCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTCATCAACATGATTCATGTTTCCTGTGGCATCTCAATTCTCGTGCTGATGGTCGTTCGTCTTTTGTTAAGGCTGAAATACCCAACCCCGCCGATTATACCTAAGCCAAAACCGATGATGACGGGACTGGCGCATTTGGGACATTTGGTGATTTATCTCCTGTTTATTGCGCTGCCAGTGATTGGTTTGGTGATGATGTATAACCGGGGCAAC  >  NC_000913/1490962‑1491231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑