Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,401,459	0	T	C	25.8%	184.0 / 21.9	97	D625G (GAT→GGT)	csrD	regulator of CsrB and CsrC decay
Reads supporting (aligned to +/- strand):  ref base T (60/12);  new base C (0/25);  total (60/37)
Fisher's exact test for biased strand distribution p-value = 1.83e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCAGACAAGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAAC‑GGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTT  >  NC_000913/3401325‑3401589                                                                                                                                        |                                                                                                                                   gCAGACAAGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAAC‑GGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCt                                                                                                                               >  1:1368389/1‑140 (MQ=255)  cAGACAAGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAAC‑GGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCtt                                                                                                                              >  1:990635/1‑140 (MQ=255)      cAAGCACCCTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATAAAAC‑GGCAGGTTAACCCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAACCCCCTTGccc                                                                                                                          <  2:1209567/140‑1 (MQ=255)      cAAGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAAC‑GGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGccc                                                                                                                          <  2:573349/140‑1 (MQ=255)      cAAGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAAC‑GGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGccc                                                                                                                          <  1:1223638/140‑1 (MQ=255)      cAAGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAAC‑GGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGccc                                                                                                                          <  2:1368389/140‑1 (MQ=255)      cAAGCACACTCACATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAAC‑GGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAACCCCCTTGccc                                                                                                                          <  2:283150/140‑1 (MQ=255)      aaaGCACCCTCCCATGAGACGCAGCGCGCATTATTCTACGTAAAAACGGATTAAAC‑GGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGccc                                                                                                                          <  2:1231106/139‑1 (MQ=255)      aaaGCACCCCCCCAGGAGACGCAGCGCGCATTATTCTACGTAAAAACGGATTAAAC‑GGCAGGTTAAACCGAGTATCTTTGTAAATATTTTTTCACGTTATTATAAAGTGGCTGGGAGGACGCGAAAAAACCCCTTTGccc                                                                                                                          <  2:1065730/139‑1 (MQ=255)      aaaGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAAC‑GGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTAAGGACGCGAAAAAATCCCCTTGccc                                                                                                                          <  1:398680/139‑1 (MQ=255)       aaGCACCCTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAAC‑GGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATAAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCg                                                                                                                         <  2:586986/140‑1 (MQ=255)             acTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAAC‑GGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTa                                                                                                                   >  1:1348589/1‑140 (MQ=255)               tCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAAC‑GGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAac                                                                                                                 >  1:964580/1‑140 (MQ=255)               tCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAAC‑GGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAac                                                                                                                 >  1:1031692/1‑140 (MQ=255)                   aTGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAAC‑GGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACcg                                                                                                             >  2:902519/1‑140 (MQ=255)                   aTGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAAC‑GGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACcg                                                                                                             >  1:1018587/1‑140 (MQ=255)                   aTGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAAC‑GGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACcg                                                                                                             >  1:666044/1‑140 (MQ=255)                     gggaggCAGGGCGAATTATTTTAGGTGAAAACGGATAAACC‑GGCCGTTTAAACGGATTTTCTTTGTAAATATTTTTTCAGGTTAGTATCAAGTGGTTGGGGGGCCGCGAAAAAACCCCCTTGCCCGCCTGTAACACcgcg                                                                                                           <  2:964580/135‑1 (MQ=255)                     gagaCGCAGCGCGCATTATTCTACGTGAAAACGGATTAAAC‑GGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATAAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACcgcg                                                                                                           <  2:1165113/140‑1 (MQ=255)                         cGCAGCGCGCATTATTCTACGTGAAAACGGATTAAAC‑GGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGa                                                                                                       >  1:32441/1‑140 (MQ=255)                         cGCAGCGCGCATTATTCTACGTGAAAACGGATTAAAC‑GGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGa                                                                                                       >  1:383576/1‑140 (MQ=255)                         cGCAGCGCGCATTATTCTACGTGAAAACGGATTAAAC‑GGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGa                                                                                                       >  2:46937/1‑140 (MQ=255)                           cAGCGCGCATTATTCTACGTGAAAACGGATTAAAC‑GGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAAt                                                                                                     >  1:1306180/1‑140 (MQ=255)                           cAGCGCGCATTATTCTACGTGAAAACGGATTAAAC‑GGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAAt                                                                                                     >  1:60918/1‑140 (MQ=255)                           cAGCGCGCATTATTCTACGTGAAAACGGATTAAAC‑GGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAAt                                                                                                     >  1:561352/1‑140 (MQ=255)                           cAGCGCGCATTATTCTACGTGAAAACGGATTAAAC‑GGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAAt                                                                                                     >  1:538102/1‑140 (MQ=255)                           cAGCGCGCATTATTCTACGTGAAAACGGATTAAAC‑GGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAAt                                                                                                     >  2:1194418/1‑140 (MQ=255)                           cAGCGCGCATTATTCTACGTGAAAACGGATTAAAC‑GGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAAt                                                                                                     >  1:258883/1‑140 (MQ=255)                           cAGCGCGCATTATTCTACGTGAAAACGGATTAAAC‑GGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTCCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAAt                                                                                                     >  1:950017/1‑140 (MQ=255)                            aGCGCGCATTATTCTACGTGAAAACGGATTAAAC‑GGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATc                                                                                                    >  1:497112/1‑140 (MQ=255)                                   attattCTACGTGAAAACGGATTAAAC‑GGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCt                                                                                             >  2:1281204/1‑140 (MQ=255)                                         ttACGTAAAAACGGATTAAAC‑GGCAGTTTAAACCGATTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACt                                                                                       <  2:1031692/139‑1 (MQ=255)                                           aCGTGAAAACGGATTAAAC‑GGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCg                                                                                     >  1:966648/1‑140 (MQ=255)                                             gTGAAAACGGATTAAAC‑GGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCt                                                                                   >  1:717596/1‑140 (MQ=255)                                              tGAAAACGGATTAAAC‑GGCAGTTAAACCCGAGTATCTTTGTGAATATTTTTTCCCTTTAGTATCAAGTGGCTGTGAGGACGCAAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCtt                                                                                  <  2:1223741/140‑1 (MQ=255)                                               gAAAACGGATTAAAC‑GGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTc                                                                                 >  1:412576/1‑140 (MQ=255)                                               gAAAACGGATTAAAC‑GGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTc                                                                                 >  1:304259/1‑140 (MQ=255)                                               gAAAACGGATTAAAC‑GGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTAGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTc                                                                                 >  2:955087/1‑140 (MQ=255)                                                 aaaCGGATTAAAC‑GGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTcgc                                                                               >  1:680964/1‑140 (MQ=255)                                                 aaaCGGATTAAAC‑GGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTcgc                                                                               >  2:919698/1‑140 (MQ=255)                                                     gggatAAACGGGCAGTTAAACCCGAGTTTTTTTGTAAATTTTTTTTCACGTTATTATAAAGTGGCTGTGAGGCCGCAAAAAAACCCCCTTCCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGaa                                                                            <  2:674765/136‑1 (MQ=255)                                                      gATTAAAC‑GGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAaca                                                                          >  1:692422/1‑139 (MQ=255)                                                       aTTAAAC‑GGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAacgc                                                                         >  2:254167/1‑140 (MQ=255)                                                               ggtaGGTAAACCCGATTATCTTGGTGAATTTTTTTTCTCGTTATTATAAAGTGGCTGTGGGGCCGCAAAAAAACCCCCTTCCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCgg                                                                  <  2:966648/137‑1 (MQ=255)                                                               ggCAGGTTAACCCGATTTTTTTTGTAAATTTTTTTTCCCGTTATTATAAAGTGGTTGGGAGGACGAAAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCgg                                                                  <  2:284171/140‑1 (MQ=255)                                                               ggCAGGTTAAACCGAGTATCTTTGTGAATTTTTTTTCACGTTAGTATCAAGTGGCTGTGAGGCCGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCgg                                                                  <  1:37652/140‑1 (MQ=255)                                                               ggCAGGTAAAGCGGATTATTTTTGGAAATTTTTTTTCACGTTATTATCAGGTGGTTGGGAGGACGCAAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCgg                                                                  <  2:666044/140‑1 (MQ=255)                                                               gccAGGTTAAACGGAGTATCTTTGTGAATTTTTTTTCCCGTTAGTATCAAGTGGCTGTGGGGACGCGAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCgg                                                                  <  2:1306180/138‑1 (MQ=255)                                                                 aaGGTTAAACCGATTATCTTTGTGAATTTTTTTTCCCTTTAGTATCAAGTGGCTGTGAGGACGCAAAAAAACCCCCTGGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGTGAACGCACGCCGGTg                                                                <  2:1121123/139‑1 (MQ=255)                                                                    gtaaacccGAGTATCTTTGTAAATATTTTTTCACGTTAGTATCAGGTGGCTGTGAGGACGCGAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCg                                                             <  2:717596/134‑1 (MQ=255)                                                                    gTTAACCCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCg                                                             <  1:787922/140‑1 (MQ=255)                                                                    gTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCg                                                             <  1:1107260/140‑1 (MQ=255)                                                                              gTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTggg                                                   >  1:683606/1‑140 (MQ=255)                                                                              gTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTggg                                                   >  2:717186/1‑140 (MQ=255)                                                                              gTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTggg                                                   >  1:470536/1‑140 (MQ=255)                                                                                 tttttGTAAATATTTTTTCAGTTTATTATAAAGTGGTGGTGAGGAGGCAAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGc                                                <  2:497112/138‑1 (MQ=255)                                                                                     tgtgAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGt                                            >  1:450512/1‑140 (MQ=255)                                                                                      gtgAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTc                                           <  2:32441/140‑1 (MQ=255)                                                                                       tgAATTTTTTTCCACGTTAGTATCAAGTGGCTGTGAGGCCGCGAAAAACCCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTcc                                          <  2:683606/140‑1 (MQ=255)                                                                                       taaaTTTTTTTTCACGTTAGTATCAAGTGGCGGTGAGGACGCAAAAAAACCCCCTTCCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTcc                                          <  2:813744/138‑1 (MQ=255)                                                                                         aaTATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCg                                        >  1:235280/1‑140 (MQ=255)                                                                                           tatTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGa                                      >  2:1204861/1‑140 (MQ=255)                                                                                           tatTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGa                                      >  2:102698/1‑140 (MQ=255)                                                                                            tttttttCCCGTTAGTATCAAGGGGTTGTGAGGACGCAAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAg                                     <  2:383576/139‑1 (MQ=255)                                                                                             ttttttCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGc                                    <  1:902519/140‑1 (MQ=255)                                                                                             ttttttCACGTTAGTATCAAGGGGCTGGGGGGCCGCAAAAAACCCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGc                                    <  2:60918/140‑1 (MQ=255)                                                                                                 tccccGTTATTATCAAGTGGTTGTGAGGCCGCGAAAAAACCCCCTTCCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGc                                <  2:235280/136‑1 (MQ=255)                                                                                                        tAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTTCTGGTCCCGGAGCAGGCTTccacc                         <  1:1194418/140‑1 (MQ=255)                                                                                                         aGTAGCAGGGGGCGGTGAGGACGAAAAAAACCCCCCTGGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTccacca                        <  2:1018587/140‑1 (MQ=255)                                                                                                                aGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTg                 >  1:584249/1‑140 (MQ=255)                                                                                                                aGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTg                 >  1:628693/1‑140 (MQ=255)                                                                                                                  tGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGaa               >  2:90380/1‑140 (MQ=255)                                                                                                                  tGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGaa               >  2:1257357/1‑140 (MQ=255)                                                                                                                  tGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGaa               <  1:955087/140‑1 (MQ=255)                                                                                                                  tGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGaa               >  2:489191/1‑140 (MQ=255)                                                                                                                    gCTGTGAGGCCGCAAAAAACTCCCCTCCCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAAcc             <  2:810646/140‑1 (MQ=255)                                                                                                                    gCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAAcc             >  2:629069/1‑140 (MQ=255)                                                                                                                    gCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAAcc             >  1:1028607/1‑140 (MQ=255)                                                                                                                    gCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAAcc             >  2:734479/1‑140 (MQ=255)                                                                                                                    gCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAAcc             >  2:173508/1‑140 (MQ=255)                                                                                                                    gCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAAcc             >  1:1219024/1‑140 (MQ=255)                                                                                                                    gCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAAcc             >  1:1260030/1‑140 (MQ=255)                                                                                                                    gCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAAcc             >  1:884800/1‑140 (MQ=255)                                                                                                                    gCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAAcc             >  1:1398788/1‑140 (MQ=255)                                                                                                                    gCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAAcc             >  1:572747/1‑140 (MQ=255)                                                                                                                    gCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAAcc             >  1:330648/1‑140 (MQ=255)                                                                                                                    gCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAAcc             >  1:1418351/1‑140 (MQ=255)                                                                                                                     cTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACTCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACca            >  2:90576/1‑140 (MQ=255)                                                                                                                     cTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACca            >  2:167208/1‑140 (MQ=255)                                                                                                                     cTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACca            >  1:333271/1‑140 (MQ=255)                                                                                                                     cTGTGAGGACGCGAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACca            <  1:1281204/140‑1 (MQ=255)                                                                                                                       gtgAGGCCGAAAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACcagc          <  2:561352/140‑1 (MQ=255)                                                                                                                       gtgAGGACGCAAAAAAACCCCCTTCCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACcagc          <  2:538102/140‑1 (MQ=255)                                                                                                                            gACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTg     >  1:1192903/1‑140 (MQ=255)                                                                                                                            gACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGCGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTg     >  1:655831/1‑140 (MQ=255)                                                                                                                             gggcgAAAAATCCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTgg    <  2:426132/138‑1 (MQ=255)                                                                                                                              cgcgAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGt   >  1:592912/1‑140 (MQ=255)                                                                                                                              cgcgAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGt   >  1:995674/1‑140 (MQ=255)                                                                                                                               gcgAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGtt  >  1:990666/1‑140 (MQ=255)                                                                                                                               gcgAAAAAACCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGtt  <  1:919698/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                   GCAGACAAGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAAC‑GGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCCGCCTGTAACACCGCGCTGAATCAGGGTCTGCCACTCGCTTCGCGAACGCACGCCGGTGGCGTAAACCTGGGTGCTGGTCCCGGAGCAGGCTTCCACCAGGCTTTGAACCAGCAGCTGGTT  >  NC_000913/3401325‑3401589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑