Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,969,654	A→G	intergenic (‑178/‑8)	ygdT ← / → mutH	hypothetical protein/DNA mismatch repair protein MutH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,969,654	0	A	G	100.0%	319.7 / NA	86	intergenic (‑178/‑8)	ygdT/mutH	hypothetical protein/DNA mismatch repair protein MutH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/55);  total (31/55)

CCCGTTGCAGTGTTGCGCAACTCGATTACCGGCAACCTAAAAAGCAATATAAATCAGAGAATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTATCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTGAAGAACAGTTGTTAGCGCAAGCACAGCAACTTTCTGGTTATACATTGGGAGAACTGGCGGCACTTGTCGGGCTGGTTACGCCA  >  NC_000913/2969519‑2969784                                                                                                                                        |                                                                                                                                   cccGTTGCAGTGTTGCGCAACTCGATTACCGGCAACCTAAAAAGCAATATAAATCAGAGAATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCAt                                                                                                                                >  2:638362/1‑140 (MQ=255) cccGTTGCAGTGTTGCGCAACTCGATTACCGGCAACCTAAAAAGCAATATAAATCAGAGAATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCAt                                                                                                                                >  1:37200/1‑140 (MQ=255) cccGTTGCAGTGTTGCGCAACTCGATTACCGGCAACCTAAAAAGCAATATAAATCAGAGAATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCAt                                                                                                                                >  1:1240727/1‑140 (MQ=255) cccGTTGCAGTGTTGCGCAACTCGATTACCGGCAACCTAAAAAGCAATATAAATCAGAGAATTGAACAACGCATGTGGAAAAAGATACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCAt                                                                                                                                >  1:751465/1‑140 (MQ=255)   cGTTGCAGTGTTGCGCAACTCGATTACCGGCAACCTAAAAAGCAATATAAATCAGAGAATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGa                                                                                                                              <  1:1388084/140‑1 (MQ=255)    gTTGCAGTGTTGCGCAACTCGATTACCGGCAACCTAAAAAGCAATATAAATCAGAGAATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGAc                                                                                                                             >  1:1747930/1‑140 (MQ=255)    gTTGCAGTGTTGCGCAACTCGATTACCGGCAACCTAAAAAGCAATATAAATCAGAGAATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGAc                                                                                                                             <  1:922502/140‑1 (MQ=255)     ttGCAGTGTTGCGCAACTCGATTACCGGCAACCTAAAAAGCAATATAAATCAGAGAATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACa                                                                                                                            >  1:1715748/1‑140 (MQ=255)     ttGCAGTGTTGCGCAACTCGATTACCGGCAACCTAAAAAGCAATATAAATCAGAGAATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACa                                                                                                                            <  1:1031655/140‑1 (MQ=255)      tGCAGTGTTGCGCAACTCGATTACCGGCAACCTAAAAAGCAATATAAATCAGAGAATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACAt                                                                                                                           <  2:1240727/140‑1 (MQ=255)       gCAGTGTTGCGCAACTCGATTACCGGCAACCTAAAAAGCAATATAAATCAGAGAATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATg                                                                                                                          >  2:290895/1‑140 (MQ=255)          gtgtTGCGCAACTCGATTACCGGCAACCTAAAAAGCAATATAAATCAGAGAATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTcc                                                                                                                       <  2:1747930/140‑1 (MQ=255)          gtgtTGCGCAACTCGATTACCGGCAACCTAAAAAGCAATATAAATCAGAGAATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTcc                                                                                                                       <  2:576483/140‑1 (MQ=255)           tgtTGCGCAACTCGATTACCGGCAACCTAAAAAGCAATATAAATCAGAGAATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTccc                                                                                                                      >  2:65927/1‑140 (MQ=255)            gtTGCGCAACTCGATTACCGGCAACCTAAAAAGCAATATAAATCAGAGAATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCa                                                                                                                     <  2:248966/140‑1 (MQ=255)            gtTGCGCAACTCGATTACCGGCAACCTAAAAAGCAATATAAATCAGAGAATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCa                                                                                                                     >  1:1360593/1‑140 (MQ=255)               gcgcAACTCGATTACCGGCAACCTAAAAAGCAATATAAATCAGAGAATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAAcc                                                                                                                  <  1:1733662/140‑1 (MQ=255)                 gcAACTCGATTACCGGCAACCTAAAAAGCAATATAAATCAGAGAATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTc                                                                                                                <  1:121059/140‑1 (MQ=255)                 gcAACTCGATTACCGGCAACCTAAAAAGCAATATAAATCAGAGAATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTc                                                                                                                <  1:966278/140‑1 (MQ=255)                   aaCTCGATTACCGGCAACCTAAAAAGCAATATAAATCAGAGAATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGc                                                                                                              <  1:290895/140‑1 (MQ=255)                   aaCTCGATTACCGGCAACCTAAAAAGCAATATAAATCAGAGAATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGc                                                                                                              <  1:1279248/140‑1 (MQ=255)                     cTCGATTACCGGCAACCTAAAAAGCAATATAAATCAGAGAATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGccc                                                                                                            <  2:1632978/140‑1 (MQ=255)                      tCGATTACCGGCAACCTAAAAAGCAATATAAATCAGAGAATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCa                                                                                                           <  2:1162141/140‑1 (MQ=255)                      tCGATTACCGGCAACCTAAAAAGCAATATAAATCAGAGAATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCa                                                                                                           <  1:1214358/140‑1 (MQ=255)                      tCGATTACCGGCAACCTAAAAAGCAATATAAATCAGAGAATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCa                                                                                                           <  1:330638/140‑1 (MQ=255)                         aTTACCGGCAACCTAAAAAGCAATATAAATCAGAGAATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTg                                                                                                        <  2:1360593/140‑1 (MQ=255)                           tACCGGCAACCTAAAAAGCAATATAAATCAGAGAATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGct                                                                                                      <  1:637301/140‑1 (MQ=255)                             ccGGCAACCTAAAAAGCAATATAAATCAGAGAATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGctct                                                                                                    <  2:212658/140‑1 (MQ=255)                               ggCAACCTAAAAAGCAATATAAATCAGAGAATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGctctct                                                                                                  >  1:1188456/1‑140 (MQ=255)                               ggCAACCTAAAAAGCAATATAAATCAGAGAATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGctctct                                                                                                  <  2:1387026/140‑1 (MQ=255)                               ggCAACCTAAAAAGCAATATAAATCAGAGAATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGctctct                                                                                                  >  1:1714639/1‑140 (MQ=255)                                  aaCCTAAAAAGCAATATAAATCAGAGAATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTctcct                                                                                               >  2:173278/1‑140 (MQ=255)                                  aaCCTAAAAAGCAATATAAATCAGAGAATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGAAATGTCCCAACCTCGCCCACAGCTCTctcct                                                                                               >  1:1199648/1‑140 (MQ=255)                                   aCCTAAAAAGCAATATAAATCAGAGAATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTctcctc                                                                                              >  1:1251476/1‑140 (MQ=255)                                    ccTAAAAAGCAATATAAATCAGAGAATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCtcctcc                                                                                             <  1:836011/140‑1 (MQ=255)                                       aaaaaGCAATATAAATCAGAGAATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGa                                                                                          <  2:1735292/140‑1 (MQ=255)                                          aaGCAATATAAATCAGAGAATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAAc                                                                                       <  2:1446287/140‑1 (MQ=255)                                            gCAATATAAATCAGAGAATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTg                                                                                     <  2:897933/140‑1 (MQ=255)                                              aaTATAAATCAGAGAATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTgaa                                                                                   <  1:345974/140‑1 (MQ=255)                                              aaTATAAATCAGAGAATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTgaa                                                                                   <  1:5208/140‑1 (MQ=255)                                              aaTATAAATCAGAGAATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTgaa                                                                                   <  1:949162/140‑1 (MQ=255)                                               atatAAATCAGAGAATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTgaag                                                                                  >  1:1677102/1‑140 (MQ=255)                                               atatAAATCAGAGAATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTgaag                                                                                  >  2:704745/1‑140 (MQ=255)                                               atatAAATCAGAGAATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTgaag                                                                                  >  1:667278/1‑140 (MQ=255)                                               atatAAATCAGAGAATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTACTCTCTCCTCCCGAAACTgaag                                                                                  >  1:1779303/1‑140 (MQ=255)                                                     aTCAGAGAATTGAACAACGCATGTGGAAAAAGATACACTGAGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTGAAGAACAgt                                                                            >  1:1215576/1‑140 (MQ=255)                                                        agagAATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTGAAGAACAgttgt                                                                         >  1:1230821/1‑140 (MQ=255)                                                         gagaATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTGAAGAACAgttgtt                                                                        <  2:280867/140‑1 (MQ=255)                                                          agaATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTGAAGAACAGTTGTTa                                                                       >  1:90450/1‑140 (MQ=255)                                                          agaATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTGAAGAACAGTTGTTa                                                                       >  2:236824/1‑140 (MQ=255)                                                            aaTTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTGAAGAACAGTTGTTAgc                                                                     >  1:1306226/1‑140 (MQ=255)                                                             aTTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTGAAGAACAGTTGTTAgcg                                                                    <  2:667278/140‑1 (MQ=255)                                                              ttGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTGAAGAACAGTTGTTAgcgc                                                                   <  1:1105685/140‑1 (MQ=255)                                                               tGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTGAAGAACAGTTGTTAGCGCa                                                                  <  2:548994/140‑1 (MQ=255)                                                                   caacGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTGAAGAACAGTTGTTAGCGCAAGca                                                              >  2:858618/1‑140 (MQ=255)                                                                      cGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTGAAGAACAGTTGTTAGCGCAAGCACAg                                                           <  1:1677642/140‑1 (MQ=255)                                                                      cGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTGAAGAACAGTTGTTAGCGCAAGCACAg                                                           >  1:252169/1‑140 (MQ=255)                                                                            tgGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTGAAGAACAGTTGTTAGCGCAAGCACAGCAACtt                                                     <  2:660133/140‑1 (MQ=255)                                                                            tgGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTGAAGAACAGTTGTTAGCGCAAGCACAGCAACtt                                                     <  2:1188456/140‑1 (MQ=255)                                                                             ggAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTGAAGAACAGTTGTTAGCGCAAGCACAGCAACttt                                                    <  2:177138/140‑1 (MQ=255)                                                                             ggAAAAAGTTACACTGCGAATATTCGACACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTGAAGAACAGTTGTTAGCGCAAGCACAGCAACttt                                                    <  1:321674/140‑1 (MQ=255)                                                                              gAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTGAAGAACAGTTGTTAGCGCAAGCACAGCAACTTTc                                                   <  2:954469/140‑1 (MQ=255)                                                                              gAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTGAAGAACAGTTGTTAGCGCAAGCACAGCAACTTTc                                                   <  2:490837/140‑1 (MQ=255)                                                                              gAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTGAAGAACAGTTGTTAGCGCAAGCACAGCAACTTTc                                                   <  2:37200/140‑1 (MQ=255)                                                                              gAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTGAAGAACAGTTGTTAGCGCAAGCACAGCAACTTTc                                                   <  1:858618/140‑1 (MQ=255)                                                                                aaaaGTTACACTGCTAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTGAAGAACAGTTGTTAGCGCAAGCACAGCAACTTTCTg                                                 <  1:1321103/140‑1 (MQ=255)                                                                                aaaaGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTGAAGAACAGTTGTTAGCGCAAGCACAGCAACTTTCTg                                                 <  1:651969/140‑1 (MQ=255)                                                                                  aaGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTGAAGAACAGTTGTTAGCGCAAGCACAGCAACTTTCTGGt                                               <  2:1230821/140‑1 (MQ=255)                                                                                       acacTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTGAAGAACAGTTGTTAGCGCAAGCACAGCAACTTTCTGGTTATAc                                          >  2:183547/1‑140 (MQ=255)                                                                                       acacTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTGAAGAACAGTTGTTAGCGCAAGCACAGCAACTTTCTGGTTATAc                                          >  2:658094/1‑140 (MQ=255)                                                                                        cacTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTGAAGAACAGTTGTTAGCGCAAGCACAGCAACTTTCTGGTTATACa                                         <  1:516914/140‑1 (MQ=255)                                                                                            gCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTGAAGAACAGTTGTTAGCGCAAGCACAGCAACTTTCTGGTTATACATTgg                                     <  2:1215576/140‑1 (MQ=255)                                                                                              gAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTGAAGAACAGTTGTTAGCGCAAGCACAGCAACTTTCTGGTTATACATTGGga                                   <  2:279138/140‑1 (MQ=255)                                                                                              gAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTGAAGAACAGTTGTTAGCGCAAGCACAGCAACTTTCTGGTTATACATTGGga                                   <  2:946133/140‑1 (MQ=255)                                                                                                       gCACATAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTGAAGAACAGTTGTTAGCGCAAGCACAGCAACTTTCTGGTTATACATTGGGAGAACTggcg                          >  1:504201/1‑140 (MQ=255)                                                                                                            tAATTGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTGAAGAACAGTTGTTAGCGCAAGCACAGCAACTTTCTGGTTATACATTGGGAGAACTGGCGGCACt                     <  2:1715748/140‑1 (MQ=255)                                                                                                               ttGCTGTTTGTTTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTGAAGAACAGTTGTTAGCGCAAGCACAGCAACTTTCTGGTTATACATTGGGAGAACTGGCGGCACTTGt                  <  1:1460100/140‑1 (MQ=255)                                                                                                                   tgtttgttTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTGAAGAACAGTTGTTAGCGCAAGCACAGCAACTTTCTGGTTATACATTGGGAGAACTGGCGGCACTTGTCggg              <  1:638362/140‑1 (MQ=255)                                                                                                                   tgtttgttTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTGAAGAACAGTTGTTAGCGCAAGCACAGCAACTTTCTGGTTATACATTGGGAGAACTGGCGGCACTTGTCggg              <  2:1677102/140‑1 (MQ=255)                                                                                                                   tgtttgttTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTGAAGAACAGTTGTTAGCGCAAGCACAGCAACTTTCTGGTTATACATTGGGAGAACTGGCGGCACTTGTCggg              <  1:183547/140‑1 (MQ=255)                                                                                                                   tgtttgttTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTGAAGAACAGTTGTTAGCGCAAGCACAGCAACTTTCTGGTTATACATTGGGAGAACTGGCGGCACTTGTCggg              <  2:504201/140‑1 (MQ=255)                                                                                                                   tgtttgttTTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTACTCTCTCCTCCCGAAACTGAAGAACAGTTGTTAGCGCAAGCACAGCAACTTTCTGGTTATACATTGGGAGAACTGGCGGCACTTGTCggg              <  2:1779303/140‑1 (MQ=255)                                                                                                                     tttgtttTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTGAAGAACAGTTGTTAGCGCAAGCACAGCAACTTTCTGGTTATACATTGGGAGAACTGGCGGCACTTGTCGGGCt            >  2:784380/1‑140 (MQ=255)                                                                                                                       tgtttTTTAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTGAAGAACAGTTGTTAGCGCAAGCACAGCAACTTTCTGGTTATACATTGGGAGAACTGGCGGCACTTGTCGGGCTgg          >  2:1335149/1‑140 (MQ=255)                                                                                                                         ttttttAATCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTGAAGAACAGTTGTTAGCGCAAGCACAGCAACTTTCTGGTTATACATTGGGAGAACTGGCGGCACTTGTCGGGCTGGtt        >  1:1576782/1‑140 (MQ=255)                                                                                                                               aaTCAAGGTGTCATGACGTGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTGAAGAACAGTTGTTAGCGCAAGCACAGCAACTTTCTGGTTATACATTGGGAGAACTGGCGGCACTTGTCGGGCTGGTTACGc    <  2:335724/138‑1 (MQ=255)                                                                                                                               aaTCAAGGTGTCATGACGTGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTGAAGAACAGTTGTTAGCGCAAGCACAGCAACTTTCTGGTTATACATTGGGAGAACTGGCGGCACTTGTCGGGCTGGTTACGc    >  1:335724/1‑138 (MQ=255)                                                                                                                               aaTCAAGGTGTCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTGAAGAACAGTTGTTAGCGCAAGCACAGCAACTTTCTGGTTATACATTGGGAGAACTGGCGGCACTTGTCGGGCTGGTTACGCCa  <  2:1030809/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                   CCCGTTGCAGTGTTGCGCAACTCGATTACCGGCAACCTAAAAAGCAATATAAATCAGAGAATTGAACAACGCATGTGGAAAAAGTTACACTGCGAATATTCGGCACATAATTGCTGTTTGTTTTTTAATCAAGGTATCATGACATGTCCCAACCTCGCCCACTGCTCTCTCCTCCCGAAACTGAAGAACAGTTGTTAGCGCAAGCACAGCAACTTTCTGGTTATACATTGGGAGAACTGGCGGCACTTGTCGGGCTGGTTACGCCA  >  NC_000913/2969519‑2969784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑