Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913646,7470TG24.1% 119.6 / 21.2 58T255P (ACC→CCC) citGtriphosphoribosyl‑dephospho‑CoA synthase
Reads supporting (aligned to +/- strand):  ref base T (1/43);  new base G (14/0);  total (15/43)
Fisher's exact test for biased strand distribution p-value = 1.48e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGT‑TC‑G‑AAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGC‑G‑CAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCA  >  NC_000913/646616‑646886
                                                                                                                                   |                                                                                                                                                 
cAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGT‑TC‑G‑AAT‑Gc                                                                                                                                       <  1:979365/140‑1 (MQ=255)
     gCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGT‑TC‑G‑AAT‑GCCCCCt                                                                                                                                  <  1:225378/140‑1 (MQ=255)
     gCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGT‑TC‑G‑AAT‑GCCCCCt                                                                                                                                  <  2:1861634/140‑1 (MQ=255)
       ttAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGT‑TC‑G‑AAT‑GCCCCCttt                                                                                                                                <  2:1415359/140‑1 (MQ=255)
       ttAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGT‑TC‑G‑AAT‑GCCCCCttt                                                                                                                                <  2:823509/140‑1 (MQ=255)
         aaaTAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGG‑TG‑G‑AAA‑GCCCCCctttt                                                                                                                              >  2:482957/1‑140 (MQ=255)
              atTAAATCTGTGCTAAAAACCAGGTAATGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGG‑TC‑GCATT‑GCCCCCTTTTTTCaa                                                                                                                          >  2:292522/1‑140 (MQ=255)
              atTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGG‑TC‑G‑AATGGCCCCCtttttctaa                                                                                                                          >  2:1451449/1‑136 (MQ=255)
              atTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGG‑TC‑G‑AAT‑GCCCCCCTTTTTCaaa                                                                                                                         >  1:1388856/1‑139 (MQ=255)
               ttAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGT‑TC‑G‑AAT‑GCCCCCTTTTTGCaata                                                                                                                        >  1:1831068/1‑140 (MQ=255)
               ttAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGT‑TC‑G‑AAT‑GCCCCCTTTTTGCaata                                                                                                                        <  2:2149782/140‑1 (MQ=255)
               ttAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGG‑TT‑G‑AAT‑GGCCCCCTTTTTCaata                                                                                                                        >  2:1864374/1‑140 (MQ=255)
                tAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGG‑TT‑G‑AAT‑GCCCCCCTTTTGCaataa                                                                                                                       >  1:56144/1‑140 (MQ=255)
                tAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGG‑TC‑G‑GAT‑GCCCCCctttttttataa                                                                                                                       >  2:659262/1‑133 (MQ=255)
                        tgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGG‑TCGG‑GTT‑GCCCCCTTTTTTCAATAATtttttg                                                                                                                >  2:1101695/1‑138 (MQ=255)
                            aaaaaCCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGT‑TC‑G‑AAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTc                                                                                                           <  2:420166/140‑1 (MQ=255)
                               aaCCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGT‑TC‑G‑AAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTcgcg                                                                                                        <  1:94967/140‑1 (MQ=255)
                                 ccAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGT‑TC‑G‑AAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCt                                                                                                      <  2:1956125/140‑1 (MQ=255)
                                 ccAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGT‑TC‑G‑AAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCt                                                                                                      <  2:1400001/140‑1 (MQ=255)
                                      tAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGT‑TC‑G‑AAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGc                                                                                                 <  2:773803/140‑1 (MQ=255)
                                           aTCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGT‑TC‑G‑AAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAgc‑g                                                                                           <  2:96502/140‑1 (MQ=255)
                                           aTCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGG‑TC‑G‑AAA‑GCCCCCCTTTTTCGATAATATTTTCGCCCCGCCCTGTAGCCAgc‑g                                                                                           >  2:941774/1‑140 (MQ=255)
                                              aGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGG‑TC‑G‑AAT‑GCCCCCTTTTTTCAATAAAGTTTTCGCCTCGCGCTGTAGCCCGC‑G‑CAg                                                                                       >  2:870037/1‑140 (MQ=255)
                                               gTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGT‑TC‑G‑CAT‑GCCCCCCTTTTTTCATAATGTTTTTGCCCCCCGCTCTAGCCCGC‑G‑CCgg                                                                                      >  2:1383709/1‑140 (MQ=255)
                                               gTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGT‑TC‑G‑AAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGC‑G‑CAgg                                                                                      <  2:1059653/140‑1 (MQ=255)
                                               gTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGG‑TT‑G‑GAT‑GCCCCCCTTTTTTAATAAAGTTTTCGCCTCGCGCTGTAGCCCGC‑G‑CAgg                                                                                      >  2:1860395/1‑140 (MQ=255)
                                               gTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGG‑TC‑G‑GAT‑GCCCCCCTTTTTCCATAATATTTTCGCCCCCCGCTCTGGCCAcc‑g‑cccg                                                                                      >  2:2207227/1‑137 (MQ=255)
                                                tAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGG‑TC‑G‑GAT‑GCCCCCCTTTTTCAATAAATTTTTCGCCTCCCGCCGTAGCCCGC‑G‑CAGGc                                                                                     >  2:1405579/1‑140 (MQ=255)
                                                 aGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGT‑TC‑G‑AAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGC‑G‑CAGGcc                                                                                    <  2:1831068/140‑1 (MQ=255)
                                                 aGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGT‑TC‑G‑AAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGC‑G‑CAGGcc                                                                                    <  2:1194700/140‑1 (MQ=255)
                                                   gTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGG‑TC‑G‑CAT‑GACCCCCTTTTGTATTTATATTTTTCCCTCCCGCCGTGGCCCCC‑G‑CCGGcccc                                                                                  >  2:1179869/1‑140 (MQ=255)
                                                    tCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGG‑TC‑G‑GAT‑TCCCCCCTTTTTCAATAATATTTTTGCCCCCCCCCGGTGCCAGC‑G‑CCGGccccc                                                                                 >  2:1093081/1‑140 (MQ=255)
                                                    tCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGG‑TC‑G‑AAT‑GCCCCCCTTTTTTTATAATATTTTTGCCTCGCGCTGTAGCCCGC‑G‑CCGGccccc                                                                                 >  2:1064845/1‑140 (MQ=255)
                                                     cAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGG‑TT‑G‑AAA‑GCGCCCCTTTTTCAATTAATTTTGTGGCTCGCGCCGTTGGCAGCGG‑CTGGccccc                                                                                 >  2:212169/1‑140 (MQ=255)
                                                     cAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGG‑TT‑G‑AAA‑GCCCCCCTTTTTCCATAATATTTTTGCCTCGCGCCGTAGCCAGC‑G‑CGCGgccccc                                                                                >  2:1431896/1‑140 (MQ=255)
                                                        cACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGG‑TC‑G‑AAT‑GCCCCCCTTTTTCAATAATGTTTTCGCCTCGCGCTGTAGCCAGC‑G‑CAGGccccccccc                                                                             >  1:1486348/1‑137 (MQ=255)
                                                        cACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGG‑TC‑G‑AAT‑GCCCCCCTTTTTCAATAATGTTTTCGCCTCCCGCTGTAGCCAGC‑G‑CAGGccccccccc                                                                             >  1:2027429/1‑137 (MQ=255)
                                                             ccgccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGG‑TC‑G‑GAT‑GCCCCCCTTTTTGAATAAATTTTTCGCCCCGCCCCGTAGCCAGC‑GCCAGGccccccccccccc                                                                         >  1:776408/1‑133 (MQ=255)
                                                             ccgccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGG‑TC‑G‑AAT‑GCCCCCCTTTTTCAATAATGTTTTCGCCTCGCGCCGTAGCCAGC‑G‑CAGGCCCCCCCCGCcccc                                                                        >  2:1507711/1‑140 (MQ=255)
                                                                ccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGT‑TC‑G‑AAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGC‑G‑CAGGCCCCCCTCGCCACcgcg                                                                     <  1:1405579/140‑1 (MQ=255)
                                                                   ggACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGT‑TC‑G‑AAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGC‑G‑CAGGCCCCCCTCGCCACCGCGCGa                                                                  <  1:1204698/140‑1 (MQ=255)
                                                                     aCTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGT‑TC‑G‑AAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGC‑G‑CAGGCCCCCCTCGCCACCGCGCGATg                                                                <  2:1644407/140‑1 (MQ=255)
                                                                                   cgATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGG‑TT‑G‑AAT‑GCCCCCCTTTTTTAATAAAGTTTTTGCCCCCCGCTGTAGCCAGC‑G‑CCGGCCCCCCCCCCCCCCCCCCGGGGCAACGGTTGGATCg                                                  >  2:187289/1‑140 (MQ=255)
                                                                                         acTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGT‑TC‑G‑AAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGC‑G‑CAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTg                                            <  1:1451449/140‑1 (MQ=255)
                                                                                           tCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGT‑TC‑G‑AAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGC‑G‑CAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGAt                                          <  2:721054/140‑1 (MQ=255)
                                                                                               tGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGT‑TC‑G‑AAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGC‑G‑CAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGcc                                      <  1:1101695/140‑1 (MQ=255)
                                                                                               tGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGT‑TC‑G‑AAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGC‑G‑CAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGcc                                      <  1:766675/140‑1 (MQ=255)
                                                                                                gTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGT‑TG‑G‑GAT‑GTCCCCCTTTTTCCATAAAGTTTTCGCCTCCCGCCGTTGGCAGC‑G‑CGGGGCCCCCCCCCCCCCGCCCCATGCGAAGCTGGGATTGCCGCTGATCGccc                                     >  2:2239605/1‑139 (MQ=255)
                                                                                                gTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGG‑TT‑G‑AAT‑GCCCCCCTTTTTCAATAAATTTTTCTCCCCGCGCTGTAGCCAGC‑C‑CAGGCCCCCCCCCCCCCCCCCCGCTGCCACGTTTGGATCGCCCTTTGTCGccc                                     >  2:341626/1‑139 (MQ=255)
                                                                                                gTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGG‑TC‑G‑GAT‑GCCCCCCTTTTTCAATAAAGTTTTCGCCCCCCCCCGTAGCCAGC‑G‑CAGGCCCCCCCCCCCCCCCCGCGATGCGACGATGGTATTCCCCGTGATCGccc                                     >  2:2077277/1‑139 (MQ=255)
                                                                                                gTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGG‑TC‑G‑AAA‑GCCCCCCTTTTTCAATAATTTTTTCGCCTCCCGCCGTAGCCAGC‑G‑CCGGCCCCCCCCCCCCCCCCCCGATGCAACGTTTGGATCGCCGGTGGTCGccc                                     >  2:1186636/1‑139 (MQ=255)
                                                                                                 tCGAACTGCCGGAGATAATCGAGATCGGCGGGGGT‑TC‑G‑AAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGC‑G‑CAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCAt                                    <  1:870037/140‑1 (MQ=255)
                                                                                                  cGAACTGCCGGAGATAATCGAGATCGGCGGAGGTGTG‑G‑ACG‑CCCCCCCTTTTGCCA‑AATTTTTTCGCCTCCCGCTCTAGTCAAC‑G‑CAAGACCCCCCCCCCCCCCCCCGGGGCGAAGTGGGGATTGCCGTTGTTCGCCATc                                   >  2:1483089/1‑140 (MQ=255)
                                                                                                    aaCTGCCGGAGATAATCGAGATCGGCGGGGGG‑TC‑GTAAT‑CCCCCCTTTTT‑TTATAAATTTTTCGCCCCGCGCTGTAGCCCGC‑G‑CAGGCCCCCCCCCCCCCCCCGCCGAGCAACCCTGGTGTCGCCGGTGGTCGCCCTcca                                 >  2:288517/1‑138 (MQ=255)
                                                                                                     aCTGCCGGAGATAATCGAGATCGGCGGGGGT‑TC‑G‑AAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGC‑G‑CAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGt                                <  1:1429082/140‑1 (MQ=255)
                                                                                                     aCTGCCGGAGATAATCGAGATCGGCGGGGGT‑TC‑G‑AAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGC‑G‑CAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGt                                <  2:776408/140‑1 (MQ=255)
                                                                                                     aCTGCCGGAGATAATCGAGATCGGCGGGGGT‑TC‑G‑AAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGC‑G‑CAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGt                                <  2:2027429/140‑1 (MQ=255)
                                                                                                     aCTGCCGGAGATAATCGAGATCGGCGGGGGG‑TC‑G‑GAT‑TCCCCCCTTTTTCAATAATATTTTCGCCTCCCGCCGTTGCCAGC‑G‑CAGGGCCCCCCCCCCCCCCCCCGGTGCAACCTTGGTATCTCCGGTGATTGCCCTCAGt                                >  2:1262890/1‑140 (MQ=255)
                                                                                                     aCTGCCGGAGATAATCGAGATCGGCGGGGGG‑TC‑G‑GAT‑GCCCCCCTTTTTCAATAATATTTTCGCCCCGCGCTGTAGCCCGC‑G‑CCGGCCCCCCCCCCCCCCCCCCGCTGCAACGGTGGGATTGCCGCTGATCGCCCTCAgg                                >  2:1775792/1‑139 (MQ=255)
                                                                                                      cTGCCGGAGATAATCGAGATCGGCGGGGGT‑TC‑G‑AAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGC‑G‑CAGG‑CCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAg                              <  1:659262/140‑1 (MQ=255)
                                                                                                       tGCCGGAGATAATCGAGATCGGCGGGGGT‑TC‑G‑AAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGC‑G‑CAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAg                              <  2:760870/140‑1 (MQ=255)
                                                                                                        gCCGGAGATAATCGAGATCGGCGGGGGT‑TC‑G‑AAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGC‑G‑CAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAgg                             <  1:1093081/140‑1 (MQ=255)
                                                                                                            gagaTAATCGAGATCGGCGGGGGT‑TC‑G‑AAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGC‑G‑CAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCa                         <  1:187289/140‑1 (MQ=255)
                                                                                                                 aaTCGAGATCGGCGGGGGT‑TC‑G‑AAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGC‑G‑CAGGCCCCCCTCGCCACCGCGCGATGCAACGTTAGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTa                    <  1:1415782/140‑1 (MQ=255)
                                                                                                                 aaTCGAGATCGGCGGGGGT‑TC‑G‑AAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGC‑G‑CAGG‑CCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTAt                   <  1:329070/140‑1 (MQ=255)
                                                                                                                   tCGAGATCGGCGGGGGG‑TT‑G‑AAT‑GCCCCCCTTTTTTAATAATTTTTTCGCCCCCCCCTGTAGCCAGC‑G‑CAGGCCCCCCCCCCCCCCCCCCGGGGCAACGGTGGTATCGCCGTTGGTCGCCCCCAGTAGGGGGAAGGGATc                  >  2:456637/1‑140 (MQ=255)
                                                                                                                     gagaTCGGCGGGGGT‑TC‑G‑AAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGC‑G‑CAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGa                <  1:1775792/140‑1 (MQ=255)
                                                                                                                       gaTCGGCGGGGGT‑TC‑G‑AAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGC‑G‑CAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGc              <  1:1490394/140‑1 (MQ=255)
                                                                                                                            gcggGGGT‑TC‑G‑AAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGC‑G‑CAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGc         <  1:1064845/140‑1 (MQ=255)
                                                                                                                            gcggGGGT‑TC‑G‑AAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGC‑G‑CAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGc         <  1:2207227/140‑1 (MQ=255)
                                                                                                                            gcggGGGT‑TC‑G‑AAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGC‑G‑CAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGc         <  1:1708508/140‑1 (MQ=255)
                                                                                                                            gcggGGGT‑TC‑G‑AAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGC‑G‑CAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGc         <  2:1630430/140‑1 (MQ=255)
                                                                                                                             cggGGGT‑TC‑G‑AAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGC‑G‑CAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGcc        <  1:1004624/140‑1 (MQ=255)
                                                                                                                                 ggT‑TC‑G‑AAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGC‑G‑CAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGtt    <  1:941774/140‑1 (MQ=255)
                                                                                                                                 ggT‑TC‑G‑AAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGC‑G‑CAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGtt    <  2:1486348/140‑1 (MQ=255)
                                                                                                                                  gT‑TC‑G‑AAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGC‑G‑CAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTc   <  1:1383709/140‑1 (MQ=255)
                                                                                                                                  gT‑TC‑G‑AAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGC‑G‑CAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTc   <  1:1179869/140‑1 (MQ=255)
                                                                                                                                   t‑tC‑G‑AAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGC‑G‑CAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCa  <  1:2077277/140‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                                 
CAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGT‑TC‑G‑AAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGC‑G‑CAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCA  >  NC_000913/646616‑646886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑