Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,001,918	0	A	C	37.8%	106.2 / 44.5	83	intergenic (‑129/+192)	fliA/fliC	RNA polymerase, sigma 28 (sigma F) factor/flagellar filament structural protein
Reads supporting (aligned to +/- strand):  ref base A (44/7);  new base C (0/31);  total (44/39)
Fisher's exact test for biased strand distribution p-value = 3.39e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCG  >  NC_000913/2001779‑2002053                                                                                                                                              |                                                                                                                                        agTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGaaaaaaa                                                                                                                                         >  2:630394/1‑140 (MQ=255)     aaTTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAAcccc                                                                                                                                     >  2:2280051/1‑140 (MQ=255)     aaTTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAAcccc                                                                                                                                     >  1:2178589/1‑140 (MQ=255)     aaTTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAAcccc                                                                                                                                     >  1:1915486/1‑140 (MQ=255)      aTTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAACCccg                                                                                                                                    >  2:940285/1‑140 (MQ=255)      aTTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAACCccg                                                                                                                                    >  1:663817/1‑140 (MQ=255)      aTTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAACCccg                                                                                                                                    >  1:788310/1‑140 (MQ=255)             aTAAACAGCCGTGGTTTATATGAGTTATGGGCATGATTACCCGTTTCTCAAGGTTTTTT‑ACTCGGACAATGAGTGGGTGAAATGAGGGGTTATTTGGGGT‑TAAAAGGAAATTCCCAGGCAAAAAAAACCCCCGCCGGTgg                                                                                                                             <  2:206440/140‑1 (MQ=255)             aTAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTgg                                                                                                                             >  2:1926822/1‑140 (MQ=255)                    gCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGaa                                                                                                                      >  1:2109918/1‑140 (MQ=255)                      ccTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGc                                                                                                                    >  1:1007628/1‑140 (MQ=255)                      ccTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGc                                                                                                                    >  1:661662/1‑140 (MQ=255)                      ccTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGc                                                                                                                    >  1:831840/1‑140 (MQ=255)                         gctttATATGAGTTATCGGCATGATTTTCCTTTTCTGCAGGTTTTTT‑AATCGGACGATTAGGGGGTGAAAGGAGGGGTTTTTGGGGGT‑TTACAGGTAAATCCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACg                                                                                                                 <  2:934270/137‑1 (MQ=255)                          tgTTATATGATTTATGGGAATTATTATCCGTTTCTGCAGGGTTTTA‑AACCGGAAGATTAGTGGGTAAAAGGGGGGGTTATTGGGGGG‑TTACAGATAAATCCCGGCCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGt                                                                                                                <  2:152618/139‑1 (MQ=255)                          cGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGt                                                                                                                >  2:1504135/1‑140 (MQ=255)                          cGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGAGAAATGAGGGGGTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGGGGAGGGGAAGCAAGt                                                                                                                >  1:374865/1‑140 (MQ=255)                               tatGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTg                                                                                                           >  1:2253843/1‑140 (MQ=255)                               tatGAGTTATCGGCATGATTATCCGATTCTGGAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAGTGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGGAGCACGttcccg                                                                                                           >  2:1506324/1‑136 (MQ=255)                                    gTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACaaa                                                                                                      >  1:920579/1‑140 (MQ=255)                                    gTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACaaa                                                                                                      >  1:1468906/1‑140 (MQ=255)                                    gTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACaaa                                                                                                      >  2:2032958/1‑140 (MQ=255)                                    gTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACaaa                                                                                                      >  1:235110/1‑140 (MQ=255)                                     ttATCGGAATGATTATCCGTTTCTGCAGGGTTTTT‑AATGGGACGATTAGGGGGTAAAAGGGGGGGTTATTTGGGGG‑TTACAGGTAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAAt                                                                                                     <  2:1602109/140‑1 (MQ=255)                                       aTCGGCATGTTTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTAAAATGAGGGTTTATTGGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTg                                                                                                   <  1:2191323/140‑1 (MQ=255)                                       aTCGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTg                                                                                                   >  1:191263/1‑140 (MQ=255)                                       aTCGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTg                                                                                                   >  1:1312291/1‑140 (MQ=255)                                       aTCGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTg                                                                                                   >  1:684870/1‑140 (MQ=255)                                       aTCGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTg                                                                                                   >  1:744178/1‑140 (MQ=255)                                         cGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTgcg                                                                                                 >  2:1359320/1‑140 (MQ=255)                                         cGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTgcg                                                                                                 >  1:2145179/1‑140 (MQ=255)                                                    cccttttCGGCAGGTTTTTA‑AATCGGACGATTAGGGGGTGAAATGGGGGGTTTTTTGGGGA‑TAACAGGAAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGc                                                                                      <  2:2253843/136‑1 (MQ=255)                                                          tCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATg                                                                                <  2:191263/140‑1 (MQ=255)                                                          tCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATg                                                                                >  2:610483/1‑140 (MQ=255)                                                           tgtgaGGGTTTTTAAATGGGACGATTGGTGGGTAAAAGGGGGGTTTATTGGGGGG‑TTCAAGTTAATTTCAAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATg                                                                                <  2:1312291/136‑1 (MQ=255)                                                           cTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgc                                                                               >  1:1282771/1‑140 (MQ=255)                                                           cTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgc                                                                               >  2:182505/1‑140 (MQ=255)                                                            tGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgcg                                                                              >  1:517935/1‑140 (MQ=255)                                                            ggcgggtttttt‑AATGGGACGATTGGGGGGTGAAAGGGGGGTTTATTGGGGGG‑TTACAGGTAAATCCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgcg                                                                              <  2:1468906/133‑1 (MQ=255)                                                                  gTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGTTTATTGGGGGG‑TTACAGGTAAATCCCAGGCAGAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTaa                                                                        <  1:383464/140‑1 (MQ=255)                                                                   ttttt‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTaaa                                                                       >  2:1277423/1‑140 (MQ=255)                                                                    tttt‑AATCGGACGATTAGTGGGTGAAATGAGGGTTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAAc                                                                      <  1:630394/140‑1 (MQ=255)                                                                    tttt‑AACCGGACGATTAGTGGGTAAAATGAGGGTTTATTGGGGGG‑TTACAGGAAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAAc                                                                      <  2:2178589/140‑1 (MQ=255)                                                                      tt‑AATCGGACGATTAGGGGGTAAAATGGGGGGTTATTGGGGGG‑TTACAGGTAAATCCCAGGCAGAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGc                                                                    <  1:1463444/140‑1 (MQ=255)                                                                            cGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTAt                                                               >  2:2060347/1‑140 (MQ=255)                                                                             ggACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATc                                                              <  1:610483/140‑1 (MQ=255)                                                                             ggACGATTAGTGGGTAAAAGGAGGGTTTATTGGGGGT‑TTAAAGGAAAATCCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATc                                                              <  2:235110/140‑1 (MQ=38)                                                                             ggACGATTAGGGGGTGAAAGGAGGGTTTATTGGGGGG‑TTACAGGTAAATTCCAGGCAAAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATc                                                              <  1:700288/140‑1 (MQ=39)                                                                              gACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATcc                                                             >  1:902206/1‑140 (MQ=255)                                                                              gACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATcc                                                             >  2:2194044/1‑140 (MQ=255)                                                                              gACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATcc                                                             >  2:499966/1‑140 (MQ=255)                                                                               gtggttAGTGGGTAAAATGGGGGGTTGTTTGGGGG‑TTACAGTTAAATTCCAGGCAGAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCg                                                            <  2:40043/136‑1 (MQ=39)                                                                               acaataaGTGGGAGAAAGGAGGGGTTATTGGGGAG‑TTACAGGTAAATTCCAGACAGAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCg                                                            <  2:744178/134‑1 (MQ=38)                                                                               aCGTTTAGTGGGTGAAATGAGGGGTTATTGGGGGG‑TTAAAGGTAAATTCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCg                                                            <  1:1178615/140‑1 (MQ=39)                                                                               aCGTTTAGGGGGTAAAATGAGGGGTTTTTGGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCg                                                            <  1:940285/140‑1 (MQ=39)                                                                                    tAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTa                                                       >  1:1339629/1‑140 (MQ=255)                                                                                      gggggTGAAAGGGGGGGTTATTGGGGGG‑TTACAGGTAAATTCCAGGAAGAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACa                                                     <  1:1066307/138‑1 (MQ=38)                                                                                        gggTGAAATG‑GGGGTTTTTTGGGGGTTTAAAGGTAAATCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaa                                                   <  2:2145179/140‑1 (MQ=37)                                                                                         ggTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaaa                                                  <  1:2060347/140‑1 (MQ=255)                                                                                         ggTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaaa                                                  >  2:1407290/1‑140 (MQ=255)                                                                                         ggTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaaa                                                  >  1:76614/1‑140 (MQ=255)                                                                                             aaaTGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAtgt                                              >  1:452453/1‑140 (MQ=255)                                                                                             aaaTGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAtgt                                              >  2:913155/1‑140 (MQ=255)                                                                                               agggggggTTATTGGGGGG‑TTACAGGTAAATCCCAGGAAGAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGc                                            <  2:661662/136‑1 (MQ=38)                                                                                               aaggggggTTATTTGGGGG‑TTACAGATAAGTTCAAGAAAAAAAAAATCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGc                                            <  2:831840/136‑1 (MQ=37)                                                                                               aTGAGGGGTTATTGGGGGG‑TTACAGGTAAATCCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGc                                            <  2:1282771/140‑1 (MQ=255)                                                                                                tGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCa                                           >  1:789354/1‑140 (MQ=255)                                                                                                 gAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTTGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCaa                                          >  1:1982104/1‑140 (MQ=255)                                                                                                 gAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCaa                                          >  2:2235093/1‑140 (MQ=255)                                                                                                       ttATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCaa                                    >  2:1575457/1‑140 (MQ=255)                                                                                                         aTTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATa                                  >  1:485447/1‑140 (MQ=255)                                                                                                             ggggg‑TTACAGGTAAATTCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAAtt                              <  2:611323/140‑1 (MQ=255)                                                                                                               ggg‑TTAAAGGAAAATTCAAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGc                            <  2:1101541/140‑1 (MQ=37)                                                                                                                     aCAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTc                       >  2:1304476/1‑140 (MQ=255)                                                                                                                           aattacgaGGCAAAAAAACCCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTg                 <  2:2109918/133‑1 (MQ=37)                                                                                                                           aaaTTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTg                 <  2:452453/140‑1 (MQ=255)                                                                                                                           aaaTTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTg                 <  1:1407290/140‑1 (MQ=255)                                                                                                                           aaaTTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTg                 <  1:1926822/140‑1 (MQ=255)                                                                                                                           aaaTTCAGGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTg                 <  2:920579/140‑1 (MQ=255)                                                                                                                           aaaTCCCAGGCAGAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTg                 <  2:384466/140‑1 (MQ=255)                                                                                                                           aaaTCCCAGGCAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTg                 <  2:902206/140‑1 (MQ=255)                                                                                                                           aaaTCCCAGGAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTg                 <  1:2280051/140‑1 (MQ=255)                                                                                                                             aTTCCAGGAAAAACAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTa               <  2:1339629/140‑1 (MQ=255)                                                                                                                                ccAGGCAGAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGc            <  1:1359320/140‑1 (MQ=255)                                                                                                                                   ggCAGAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAg              <  1:1257091/135‑1 (MQ=255)                                                                                                                                   ggCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAg              >  2:1257091/1‑135 (MQ=255)                                                                                                                                          aaaacCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcg  <  2:789354/140‑1 (MQ=255)                                                                                                                                              |                                                                                                                                        AGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTT‑AATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGG‑TTACAGGTAAATTCCAGGCAGAAAAAAACCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCG  >  NC_000913/2001779‑2002053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑