Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,240,9850TG24.8% 209.2 / 23.1 101S348A (TCT→GCT) sstTserine/threonine:Na(+) symporter
Reads supporting (aligned to +/- strand):  ref base T (15/61);  new base G (25/0);  total (40/61)
Fisher's exact test for biased strand distribution p-value = 1.25e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 113 total aligned reads displayed.

GGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTG  >  NC_000913/3240846‑3241122
                                                                                                                                            |                                                                                                                                          
gggcgCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGt                                                                                                                                            <  1:2135118/140‑1 (MQ=255)
gggcgCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGt                                                                                                                                            <  1:1347359/140‑1 (MQ=255)
     cagcaATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTctg                                                                                                                                       <  1:1026396/140‑1 (MQ=255)
        caATCTCTATTTCCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGGTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCAACCGGCGTGGCGGGGGGGTCTctgctg                                                                                                                                    <  2:1785113/140‑1 (MQ=255)
            cACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGa                                                                                                                               <  1:1914301/140‑1 (MQ=255)
            cACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGa                                                                                                                               <  2:1733537/140‑1 (MQ=255)
                 ttACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCa                                                                                                                          <  1:1280592/140‑1 (MQ=255)
                 ttACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCa                                                                                                                          <  2:1190620/140‑1 (MQ=255)
                 ttACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCa                                                                                                                          <  2:634808/140‑1 (MQ=255)
                 ttACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCACATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCa                                                                                                                          <  1:2179422/140‑1 (MQ=255)
                 ttACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCATACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCa                                                                                                                          <  1:181969/140‑1 (MQ=255)
                 atgccGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCa                                                                                                                          <  2:1605212/137‑1 (MQ=255)
                  tACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCAc                                                                                                                         >  2:1590579/1‑140 (MQ=255)
                  tACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTTTGCTGC‑TTAAccccc                                                                                                                         >  2:2250623/1‑138 (MQ=255)
                  tACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGC‑TGATCCCAc                                                                                                                         >  1:688811/1‑140 (MQ=255)
                  tACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGC‑TGATCCCAc                                                                                                                         >  1:412863/1‑140 (MQ=255)
                   aCCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACt                                                                                                                        <  1:293585/140‑1 (MQ=255)
                   aCCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACt                                                                                                                        <  1:1898245/140‑1 (MQ=255)
                   aCCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACt                                                                                                                        <  1:967977/140‑1 (MQ=255)
                   aCCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACt                                                                                                                        <  1:2219630/140‑1 (MQ=255)
                   aCCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACt                                                                                                                        <  1:335852/140‑1 (MQ=255)
                   aCCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGAGGCGCATCCGGCGTGGCGGGGGGGTCTTTGCTGC‑TGATCCCACt                                                                                                                        <  1:1099411/140‑1 (MQ=255)
                     cGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGTATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACTgg                                                                                                                      <  1:44714/140‑1 (MQ=255)
                     cGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACTgg                                                                                                                      <  1:108526/140‑1 (MQ=255)
                     cGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGC‑TGATCCCACTgg                                                                                                                      >  1:393515/1‑140 (MQ=255)
                      gtgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACTGGc                                                                                                                     <  1:1480083/140‑1 (MQ=255)
                      gtgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACTGGc                                                                                                                     <  1:1093910/140‑1 (MQ=255)
                      gtgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACTGGc                                                                                                                     <  1:1948399/140‑1 (MQ=255)
                      gtgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGC‑TGATCCCACTGGc                                                                                                                     >  2:1850564/1‑140 (MQ=255)
                       tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACTGGcc                                                                                                                    >  2:1719951/1‑140 (MQ=255)
                       tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGC‑TGATCCCACTGGcc                                                                                                                    >  2:785604/1‑140 (MQ=255)
                       tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGC‑TGATCCCACTGGcc                                                                                                                    >  2:1249445/1‑140 (MQ=255)
                         ttGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACTGGCCTg                                                                                                                  >  2:1590247/1‑140 (MQ=255)
                         ttGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGC‑TGATCCCACTGGc                                                                                                                     >  1:1773060/1‑137 (MQ=255)
                         ttGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGC‑TGATCCCACTGGCCTg                                                                                                                  >  1:897414/1‑140 (MQ=255)
                         ttGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGC‑TGATCCCACTGGCCTg                                                                                                                  >  1:961901/1‑140 (MQ=255)
                                 ggctgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACTGGCCTGt                                                                                                                 <  1:936663/133‑1 (MQ=255)
                                 ggctgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGC‑TGATCCCACTGGCCTGt                                                                                                                 >  2:936663/1‑133 (MQ=255)
                                   ctgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTc                                                                                                        <  1:516456/140‑1 (MQ=255)
                                   ctgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGG‑GCTGCGTTTGAGCGTGGTGGGTTCTCTGAGCTGCTTG‑GGCGCCGCCGGCCGGGCGGGGGGGTTTCCTCTTCTTAATCCCCCTGACC‑GTAATATGATcc                                                                                                       >  2:2044896/1‑139 (MQ=255)
                                    tgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCg                                                                                                       <  1:1893230/140‑1 (MQ=255)
                                    tgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCg                                                                                                       >  2:589178/1‑140 (MQ=255)
                                            aTACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCg                                                                                               >  1:1605295/1‑140 (MQ=255)
                                              aCGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGaa                                                                                             <  2:1217335/140‑1 (MQ=255)
                                              aCGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGaa                                                                                             <  2:393515/140‑1 (MQ=255)
                                                   gggTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGata                                                                                        >  1:1401847/1‑140 (MQ=255)
                                                   gggTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGata                                                                                        >  1:1193224/1‑140 (MQ=255)
                                                   gggTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGata                                                                                        >  2:1531322/1‑140 (MQ=255)
                                                      tATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGC‑TGATCCCACTGGCCTGTAA‑ATGTTCGGTATTTCGAACGATATcac                                                                                    >  1:552276/1‑138 (MQ=255)
                                                         tCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCa                                                                                  <  1:2227655/140‑1 (MQ=255)
                                                         tCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCa                                                                                  >  1:54032/1‑140 (MQ=255)
                                                           cGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAATGATATCGCCATg                                                                                <  1:652107/140‑1 (MQ=255)
                                                             gTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCa                                                                              <  1:381056/140‑1 (MQ=255)
                                                              tCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAg                                                                             >  2:190712/1‑140 (MQ=255)
                                                              tCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAg                                                                             >  1:68800/1‑140 (MQ=255)
                                                              tCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAg                                                                             >  2:770103/1‑140 (MQ=255)
                                                                gATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAggt                                                                           >  1:1233760/1‑140 (MQ=255)
                                                                  tCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAggtgg                                                                         <  2:961901/140‑1 (MQ=255)
                                                                       ccACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGcc                                                                    <  1:1129822/140‑1 (MQ=255)
                                                                        cACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACTTGCCTGTAATATGTTCGGTATTTTGAACGATATCGCCATGCCGGTGGTTGCCg                                                                   >  1:710376/1‑140 (MQ=255)
                                                                        cACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCg                                                                   <  1:2250623/140‑1 (MQ=255)
                                                                        cACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCg                                                                   <  1:1590579/140‑1 (MQ=255)
                                                                         aCGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGt                                                                  <  1:1719951/140‑1 (MQ=255)
                                                                         aCGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGt                                                                  <  1:1850564/140‑1 (MQ=255)
                                                                           ggCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCCTGCAGGTGGGTGGCGTCg                                                                >  2:219754/1‑140 (MQ=255)
                                                                           ggCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCg                                                                >  1:480596/1‑140 (MQ=255)
                                                                           ggCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCg                                                                >  2:706750/1‑140 (MQ=255)
                                                                                tgctgTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGC‑TGATCCCACTCGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCttt                                                           >  1:1473727/1‑140 (MQ=255)
                                                                                      tGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTatcatc                                                     <  1:2033206/140‑1 (MQ=255)
                                                                                         gCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGc                                                  <  2:2223624/140‑1 (MQ=255)
                                                                                          cGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTTCTGC‑TGATCCCACTGGCCTGTAATATTTTCTGTATTTTGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCg                                                 >  1:1433755/1‑140 (MQ=255)
                                                                                          cGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCCCTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCg                                                 >  1:1901306/1‑140 (MQ=255)
                                                                                          cGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACTGGCCTGTGATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCg                                                 <  1:587907/140‑1 (MQ=255)
                                                                                          cGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCg                                                 <  2:68800/140‑1 (MQ=255)
                                                                                            tggtggCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTa                                               <  1:1679405/140‑1 (MQ=255)
                                                                                            tggtggCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTa                                               >  2:1776547/1‑140 (MQ=255)
                                                                                            tggtggCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGGGGTTGCCGTCGGCTTTATCATCGGCGTa                                               >  1:2164900/1‑140 (MQ=255)
                                                                                            tggtggCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGCGTCTGCTGC‑TGCTCCCCACGGCCGGTAATATGGTCGGTATTTTGAACGGTATTGCCCTGGAGGGGGGTGGCGTCGGCTTTATTATCGGGGtg                                               >  2:298837/1‑139 (MQ=255)
                                                                                                   ttCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAgg                                        <  2:268313/140‑1 (MQ=255)
                                                                                                               ccTGTGGCGCATCCGGCGTGGCGGGGGGGGCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGaa                             >  2:202003/1‑140 (MQ=255)
                                                                                                                     gcgcATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGGTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACcgcg                       >  1:1009127/1‑140 (MQ=255)
                                                                                                                     gcgcATCCGGCGTGGCGGGGGGGGCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACcgcg                       >  1:2222636/1‑140 (MQ=255)
                                                                                                                        cATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTg                    <  1:734876/140‑1 (MQ=255)
                                                                                                                        cATCCGGCGTGGCGGGGGGGGCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTg                    >  2:353225/1‑140 (MQ=255)
                                                                                                                          tCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGaa                  <  1:1531322/140‑1 (MQ=255)
                                                                                                                             ggCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAActc               <  2:54032/140‑1 (MQ=255)
                                                                                                                             ggCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAActc               <  2:1193224/140‑1 (MQ=255)
                                                                                                                               cGTGGCGGGGGGGGCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCtt             >  1:1773573/1‑140 (MQ=255)
                                                                                                                               cGTGGCGGGGGGGGCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGGGGGTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCtt             >  1:1011470/1‑140 (MQ=255)
                                                                                                                                gTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTc            <  2:1233760/140‑1 (MQ=255)
                                                                                                                                gTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTc            <  1:770103/140‑1 (MQ=255)
                                                                                                                                gTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTc            <  1:405506/140‑1 (MQ=255)
                                                                                                                                   gCGGGGGGGTCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAAc         >  1:727979/1‑140 (MQ=255)
                                                                                                                                   gCGGGGGGGTCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAAc         <  1:1776547/140‑1 (MQ=255)
                                                                                                                                   gCGGGGGGGGCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAAc         >  1:1945177/1‑140 (MQ=255)
                                                                                                                                     gggggggTCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTg       <  2:1433755/140‑1 (MQ=255)
                                                                                                                                     gggggggTCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTg       <  2:480596/140‑1 (MQ=255)
                                                                                                                                     gggggggTCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTg       <  1:589178/140‑1 (MQ=255)
                                                                                                                                       gggggTCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGAc     <  1:821816/140‑1 (MQ=255)
                                                                                                                                       gggggTCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGAc     <  1:574547/140‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                          
GGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTG‑TGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGC‑TGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTG  >  NC_000913/3240846‑3241122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑