Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,098,1550AC21.3% 332.1 / 16.2 151L140F (TTA→TTC) rhaSDNA‑binding transcriptional activator RhaS
Reads supporting (aligned to +/- strand):  ref base A (69/49);  new base C (0/32);  total (69/82)
Fisher's exact test for biased strand distribution p-value = 8.52e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 159 total aligned reads displayed.

TTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATG  >  NC_000913/4098016‑4098294
                                                                                                                                            |                                                                                                                                           
ttCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTa                                                                                                                                             <  1:482807/140‑1 (MQ=255)
   tcGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTAccc                                                                                                                                          >  1:2043292/1‑140 (MQ=255)
   tcGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTAccc                                                                                                                                          >  1:1857816/1‑140 (MQ=255)
          gCTGAATCGGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCg                                                                                                                                   <  2:318157/140‑1 (MQ=255)
          gCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGA‑AAAATGATTTACCCTCGACCg                                                                                                                                   <  2:396191/140‑1 (MQ=255)
          gCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAG‑AGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTCCCCTCGACCGc                                                                                                                                  <  2:395/140‑1 (MQ=255)
           cTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCTTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTCCCCTCGACCGc                                                                                                                                  <  2:1698315/140‑1 (MQ=255)
            tGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTACACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGcc                                                                                                                                 <  2:1321403/140‑1 (MQ=255)
              aaTCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTCCCCTCGACCGCCAg                                                                                                                               <  2:662405/140‑1 (MQ=255)
                tCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTCCCCTCGACCGCCAGTc                                                                                                                             <  1:351565/140‑1 (MQ=255)
                 gatttcctgcCCCAAGACCTGGGTGGGCAGTAACCCTCTATATGGCCCTTTATCCCCTTCGTATTGCAGCAGGGGCGATAATTCGTCACACAGATGAAACGGCAGAAAGGGG‑AAAATGATTTACCCTCGTCCGCCAGTcg                                                                                                                            <  2:1297175/134‑1 (MQ=255)
                 cAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTCCCCTCGACCGCCAGTcg                                                                                                                            <  2:737999/140‑1 (MQ=255)
                  gtttGCTGCCACAATAGCTGGTTGGGGATTTTCCGTCTCCCGGGGGCGTTAACCACAGCTTTTTGCAGCAGGTGCGACAGGTGGTTGCAAGGAGGGAACAGCAGGAGGGGA‑AAAATGATTTCCCCTCGACCGCCAGTcgc                                                                                                                           <  2:2227677/138‑1 (MQ=255)
                  aGTTGCTGCCACAAGAGCTGTATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTCCCCTCGACCGCCAGTcgc                                                                                                                           <  2:1777839/140‑1 (MQ=255)
                  aGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTCCCCTCGACCGCCAGTcgc                                                                                                                           <  1:323956/140‑1 (MQ=255)
                  aGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTcgc                                                                                                                           <  1:1205781/140‑1 (MQ=255)
                   gTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTcgcg                                                                                                                          <  1:1638259/140‑1 (MQ=255)
                   gTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTcgcg                                                                                                                          <  1:20583/140‑1 (MQ=255)
                   gTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTcgcg                                                                                                                          <  1:2012195/140‑1 (MQ=255)
                    ttGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCga                                                                                                                         >  1:623287/1‑140 (MQ=255)
                    ttGCTGCCAAAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACGGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCga                                                                                                                         <  1:1968910/140‑1 (MQ=255)
                      gctgcCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCgaga                                                                                                                       >  1:1942277/1‑140 (MQ=255)
                       ctgcCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGAt                                                                                                                      <  2:1233925/140‑1 (MQ=255)
                        tgcCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATc                                                                                                                     >  2:1632778/1‑140 (MQ=255)
                         gcCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCa                                                                                                                                <  1:1311060/128‑1 (MQ=255)
                         gcCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGGTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGGGATCt                                                                                                                    >  1:176854/1‑140 (MQ=255)
                             caAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGtt                                                                                                                <  1:533168/140‑1 (MQ=255)
                                gagCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTAt                                                                                                             <  1:850758/140‑1 (MQ=255)
                                 agCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATg                                                                                                            <  1:191599/140‑1 (MQ=255)
                                 agCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATg                                                                                                            >  1:266276/1‑140 (MQ=255)
                                  gCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGc                                                                                                           <  2:411621/140‑1 (MQ=255)
                                  gCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTcg                                                                                                                            >  2:1274125/1‑123 (MQ=255)
                                  gCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGc                                                                                                           <  2:1347000/140‑1 (MQ=255)
                                    tGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCaa                                                                                                         >  1:2178550/1‑140 (MQ=255)
                                    tGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCaa                                                                                                         >  2:1493874/1‑140 (MQ=255)
                                    tGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCaa                                                                                                         >  1:530384/1‑140 (MQ=255)
                                     ggATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAAt                                                                                                        <  2:1857816/140‑1 (MQ=255)
                                      gATGGGCATTACCCGTCTCCCTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAAtt                                                                                                       <  1:1598007/140‑1 (MQ=255)
                                      gATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAA‑GGG‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTa                                                                                                      <  1:2215428/140‑1 (MQ=255)
                                                aTCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTtg                                                                                             >  2:1512518/1‑140 (MQ=255)
                                                aTCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTtg                                                                                             >  2:1729442/1‑140 (MQ=255)
                                                 tCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTtgc                                                                                            >  2:1953547/1‑140 (MQ=255)
                                                   cGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTtgctg                                                                                          <  2:1707042/140‑1 (MQ=255)
                                                     tctcACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCg                                                                                        >  2:323607/1‑140 (MQ=255)
                                                     tctcACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCg                                                                                        >  2:435978/1‑140 (MQ=255)
                                                     tctcACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCg                                                                                        >  1:232942/1‑140 (MQ=255)
                                                     tctcACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCg                                                                                        >  1:2082860/1‑140 (MQ=255)
                                                      ctcACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCCCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGt                                                                                       <  2:680235/140‑1 (MQ=255)
                                                         aCTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTaaa                                                                                    >  2:906882/1‑140 (MQ=255)
                                                         aCTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTaaa                                                                                    >  1:2228741/1‑140 (MQ=255)
                                                           tGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAg                                                                                  <  1:236152/140‑1 (MQ=255)
                                                           tGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAg                                                                                  <  1:2147803/140‑1 (MQ=255)
                                                               gcgTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGt                                                                              <  2:266086/140‑1 (MQ=255)
                                                                  ttAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAAAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGttt                                                                            >  2:270910/1‑140 (MQ=255)
                                                                  ttAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTg                                                                           >  1:1290006/1‑140 (MQ=255)
                                                                       cacgGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGga                                                                      <  1:145583/140‑1 (MQ=255)
                                                                       cacaGCTTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGA‑AAAATGATTTCCCCTCAACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGga                                                                      <  1:1974403/140‑1 (MQ=255)
                                                                         caGCGTATTGCAGCGGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAGGGGA‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGgaga                                                                    <  2:2271174/140‑1 (MQ=255)
                                                                         caGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGgaga                                                                    <  2:1084749/140‑1 (MQ=255)
                                                                           gCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAAc                                                                  <  2:78412/140‑1 (MQ=255)
                                                                           gCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAAc                                                                  >  2:1718866/1‑140 (MQ=255)
                                                                              tATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAACTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTg                                                               <  2:2043292/140‑1 (MQ=255)
                                                                               aTTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGgaga                                                                    >  2:322149/1‑134 (MQ=255)
                                                                               aTTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTgg                                                              >  2:513534/1‑140 (MQ=255)
                                                                               aTTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTgg                                                              >  2:1872206/1‑140 (MQ=255)
                                                                               aTTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTgg                                                              >  2:608716/1‑140 (MQ=255)
                                                                               aTTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTgg                                                              >  2:1496973/1‑140 (MQ=255)
                                                                               aTTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCCCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTgg                                                              >  2:736984/1‑140 (MQ=255)
                                                                                 tGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGaa                                                            >  1:993126/1‑140 (MQ=255)
                                                                                           gCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCAt                                                  <  2:1737501/140‑1 (MQ=255)
                                                                                           gCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCAt                                                  >  1:1110765/1‑140 (MQ=255)
                                                                                           gCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCAt                                                  >  1:1313780/1‑140 (MQ=255)
                                                                                           gCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCAt                                                  >  1:1356814/1‑140 (MQ=255)
                                                                                            cGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATc                                                 <  2:1593339/140‑1 (MQ=255)
                                                                                            cGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATc                                                 <  2:2165476/140‑1 (MQ=255)
                                                                                                   tGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCCGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGtctc                                          >  1:692191/1‑140 (MQ=255)
                                                                                                       tGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACt                                      <  2:2255787/140‑1 (MQ=255)
                                                                                                            aGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCtt                                 >  2:86549/1‑140 (MQ=255)
                                                                                                            aGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCtt                                 <  2:764768/140‑1 (MQ=255)
                                                                                                                 gAACAGCAGGAAGGGG‑AAAATGATTTCCCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctggc                            <  2:510493/140‑1 (MQ=255)
                                                                                                                 gAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctggc                            >  2:94770/1‑140 (MQ=255)
                                                                                                                 gAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTctggc                            <  1:1755950/140‑1 (MQ=255)
                                                                                                                        agGAAGGGA‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCctggctg                     <  1:94770/140‑1 (MQ=255)
                                                                                                                           aaGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTggag                  <  1:435978/140‑1 (MQ=255)
                                                                                                                            aGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTggagg                 >  2:1023841/1‑140 (MQ=255)
                                                                                                                              ggg‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGAc               >  1:2023728/1‑140 (MQ=255)
                                                                                                                               gg‑AAAATGATTCACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGAcc              >  2:810564/1‑140 (MQ=255)
                                                                                                                                g‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCa             <  2:2178550/140‑1 (MQ=255)
                                                                                                                                  aaaaTGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCAt            >  1:2119982/1‑140 (MQ=255)
                                                                                                                                  aaaaTGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCAt            >  1:1670213/1‑140 (MQ=255)
                                                                                                                                  aaaaTGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCAt            >  1:1496043/1‑140 (MQ=255)
                                                                                                                                  aaaaTGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCAt            >  2:16179/1‑140 (MQ=255)
                                                                                                                                   aaaTGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCAtt           <  1:1289550/140‑1 (MQ=255)
                                                                                                                                     aTGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCAtttt         >  1:1123600/1‑140 (MQ=255)
                                                                                                                                         tttACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCg     >  2:1637868/1‑140 (MQ=255)
                                                                                                                                          ttACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGa    >  2:2031738/1‑140 (MQ=255)
                                                                                                                                          ttACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGa    >  2:47291/1‑140 (MQ=255)
                                                                                                                                           tACCCTCGCCCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGAt   <  2:176854/140‑1 (MQ=255)
                                                                                                                                           tACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGAt   <  1:367558/140‑1 (MQ=255)
                                                                                                                                            aCCCTCGACCGCTAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATg  >  1:101698/1‑140 (MQ=255)
                                                                                                                                            |                                                                                                                                           
TTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATCCGTCTCACTGGCGCGTTAACCACAGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGG‑AAAATGATTTACCCTCGACCGCCAGTCGCGAGATCTTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGAGAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATG  >  NC_000913/4098016‑4098294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑