Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,429,9840TG21.9% 222.4 / 22.4 96L41V (TTG→GTG) cycAserine/alanine/glycine/cycloserine:H(+)symporter
Reads supporting (aligned to +/- strand):  ref base T (13/62);  new base G (21/0);  total (34/62)
Fisher's exact test for biased strand distribution p-value = 1.19e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 128 total aligned reads displayed.

GGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTT  >  NC_000913/4429845‑4430122
                                                                                                                                           |                                                                                                                                            
ggTGCAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGgt                                                                                                                                              <  2:368073/140‑1 (MQ=255)
ggTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGgt                                                                                                                                              <  1:1831798/140‑1 (MQ=255)
ggTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGgt                                                                                                                                              <  1:1055617/140‑1 (MQ=255)
ggTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGgt                                                                                                                                              <  1:1128865/140‑1 (MQ=255)
ggTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGgt                                                                                                                                              <  2:1237941/140‑1 (MQ=255)
 gTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGgtt                                                                                                                                             >  2:1298640/1‑140 (MQ=255)
 gTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGGACGGGgtt                                                                                                                                             >  2:386097/1‑140 (MQ=255)
   aCAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGgttg‑t                                                                                                                                          <  1:195722/140‑1 (MQ=255)
   aCAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGgttg‑t                                                                                                                                          <  1:1302649/140‑1 (MQ=255)
       gaagaaAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTG‑TTTAt                                                                                                                                      <  2:1305022/140‑1 (MQ=255)
         agaaAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGGCATTTGGTCGGGGGTG‑ttttttg                                                                                                                                    >  2:1831798/1‑136 (MQ=255)
         agaaAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTG‑TTTATgg                                                                                                                                    >  2:1260472/1‑140 (MQ=255)
           aaaaaaaCATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCCTTGGGACGGGGGTT‑TTTATgggg                                                                                                                                  >  1:566946/1‑140 (MQ=255)
           aaaaaaaCATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTG‑TTTATgggg                                                                                                                                  >  2:1255687/1‑140 (MQ=255)
           aaaaaaaCATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGCGGCCTTGGTACGGGGCTG‑TTTTAgggg                                                                                                                                  >  2:1769375/1‑140 (MQ=255)
                   aTGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTG‑TTTATGGGGTCTGGCaa                                                                                                                          <  2:1278121/140‑1 (MQ=255)
                   aTGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTG‑TTTATGGGGTCTGGCaa                                                                                                                          <  2:1984639/140‑1 (MQ=255)
                     ggTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGACTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGGACGGGGGTG‑TTTTTGGGGTCTGGCaaaa                                                                                                                        >  2:660248/1‑140 (MQ=255)
                       tAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTACCATTGGTACGGGGTTG‑TTTATGGGGTCTGGCAAAACg                                                                                                                      <  1:1047343/140‑1 (MQ=255)
                            cAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCCATGGGACGGGGGTG‑TTTTTTGGGGCTGGCAAAAAGATTaa                                                                                                                 >  2:1274728/1‑139 (MQ=255)
                             aGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTG‑TTTATGGGGTCTGGCAAAACGATTAGc                                                                                                                <  2:945135/140‑1 (MQ=255)
                               gTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTG‑TTTATGGGGTCTGGCAAAACGATTAGCCt                                                                                                              >  2:1015873/1‑140 (MQ=255)
                               gTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTG‑TTTATGGGGGCTGGCAAAACGATTAGCCt                                                                                                              >  1:1156981/1‑140 (MQ=255)
                                 aaaaGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTG‑TTTTTGGGGGCTGGCAAAAAGATTAGCCttt                                                                                                            >  2:1420750/1‑139 (MQ=255)
                                   aaGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTGCGGGGGTG‑TTTTTGGGGGCTGGCAAAACGGTTAGCCTTG‑cc                                                                                                         >  1:1062885/1‑140 (MQ=255)
                                    aGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCg                                                                                                        >  1:1366857/1‑140 (MQ=255)
                                    aGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTG‑TTTATGGGGTCTAGCAAAACGATTAGCCTTG‑CCg                                                                                                        <  1:1991919/140‑1 (MQ=255)
                                    aGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTG‑TTTATGGGGGCTGGCAAAACGATTAGCCTTT‑CCg                                                                                                        >  2:640581/1‑140 (MQ=255)
                                    aGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGGACGGGGGTG‑TTTATTGGGGCTGGCAAAAAGATTAGCCTTG‑CCg                                                                                                        >  2:594435/1‑140 (MQ=255)
                                     gTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCgg                                                                                                       <  2:646127/140‑1 (MQ=255)
                                      tCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGGACGGGGGTG‑TTTATGGGGGCTGGCAAAACGATTAGCCTTT‑CCggg                                                                                                      >  2:64015/1‑140 (MQ=255)
                                         ttcccGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGGGCCCTTTGTACGGGGGTG‑GTTTTGGGGGCTGGGAAAACGATTAGCCTTGCCCGGGcc                                                                                                    >  2:513252/4‑140 (MQ=255)
                                         ttGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCCTTGGTACGGGGGTG‑TTTTTTGGGGCTGGCAAAACGATTAGCCTTT‑CCGGGGCg                                                                                                   >  2:1056653/1‑140 (MQ=255)
                                         ttGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTG‑TTTATGGGGTCTGGCAAAACGAt                                                                                                                    <  2:1842353/124‑1 (MQ=255)
                                         ttGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTG‑TTTATGGGGTCTGGCAAAACGAt                                                                                                                    >  1:1842353/1‑124 (MQ=255)
                                         ttGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCg                                                                                                   <  2:2236417/140‑1 (MQ=255)
                                         ttGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCg                                                                                                   >  1:200248/1‑140 (MQ=255)
                                         ttGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTG‑TTTTTTGGGGCTGGCAAAACGATTAGCCTTT‑GCGGGCCg                                                                                                   >  2:255602/1‑140 (MQ=255)
                                         ttGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTG‑TTTTTGGGGGCCGGCCAAACGATTAGCCTTGCCCGGGcc                                                                                                    >  2:677775/1‑140 (MQ=255)
                                         ttGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCg                                                                                                   >  1:49549/1‑140 (MQ=255)
                                              gatgatCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGAt                                                                                              <  1:241212/140‑1 (MQ=255)
                                               atgatCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTG‑TTTTTTGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGGTc                                                                                             >  2:614115/1‑140 (MQ=255)
                                                    cAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGATCAtttt                                                                                        <  2:1091091/140‑1 (MQ=255)
                                                    cAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGATCAtttt                                                                                        <  1:1298640/140‑1 (MQ=255)
                                                    cAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGATCAtttt                                                                                        <  1:677775/140‑1 (MQ=255)
                                                    cAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGATCAtttt                                                                                        <  2:549390/140‑1 (MQ=255)
                                                      ggCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGATCATTTTCg                                                                                      <  1:1856519/140‑1 (MQ=255)
                                                      ggCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGATCATTTTCg                                                                                      <  1:1109175/140‑1 (MQ=255)
                                                      ggCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTG‑TTTTTTGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGGCGTCGATCAtttttg                                                                                      >  1:247898/1‑138 (MQ=255)
                                                       gCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGATCATTTTCGt                                                                                     <  1:640581/140‑1 (MQ=255)
                                                        cTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGTTCATTTTCGtt                                                                                    <  2:2000414/140‑1 (MQ=255)
                                                         tCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCCTTTGTACGGGGGTG‑TTTTTGGGGGCTGGGAAAACGATTTGCCTTG‑CCGGGCCGGCGGTTATTTTTGttt                                                                                   >  1:1612022/1‑140 (MQ=255)
                                                         tCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGATCATTTTCGttt                                                                                   <  1:255602/140‑1 (MQ=255)
                                                           cGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTG‑TTTATGGGGGCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGATCATTTTCGTTtat                                                                                 >  1:1993749/1‑140 (MQ=255)
                                                            ggCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGATCATTTTCGTTtata                                                                                <  2:2229177/140‑1 (MQ=255)
                                                            ggCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGATCATTTTCGTTtata                                                                                <  1:2258480/140‑1 (MQ=255)
                                                             gCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGATCATTTTCGTTTatat                                                                               <  2:260694/140‑1 (MQ=255)
                                                              cTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGATCATTTTCGTTTATATg                                                                              <  1:355388/140‑1 (MQ=255)
                                                                   cAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGATCATTTTCGTTTATATGATCAt                                                                         <  1:1519672/140‑1 (MQ=255)
                                                                   cAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTACCATTGGTACGGGGTTG‑TTTATGGGGTCCGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGATCATTTTCGTTTATATGATCAt                                                                         <  1:1260472/140‑1 (MQ=255)
                                                                     gTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGATCATTTTCGTTTATATGATCATTg                                                                       <  1:1806817/140‑1 (MQ=255)
                                                                     gTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGATCATTTTCGTTTATATGATCATTg                                                                       <  1:1255687/140‑1 (MQ=255)
                                                                      tCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGTCCTTTGGACGGGGGTGTTTTTTGGGGTTTGG‑AAAACGAGTAACCCTT‑TCGGGGCGTCGGGCATTTTTGTTTTTATGTGCAtttg                                                                      >  2:535727/1‑138 (MQ=255)
                                                                      tCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCCTTTGTACGGGGGTG‑TTTTTGGGGTCTGGCAAAACGATTAGCCTTT‑CCGGGCCGTCGATCATTTTTGTTTTTATGATCAtttg                                                                      >  2:104772/1‑138 (MQ=255)
                                                                      tCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGATCATTTTCGTTTATATGATCATTgg                                                                      <  1:660248/140‑1 (MQ=255)
                                                                            cGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTAt                                                                <  1:1420750/140‑1 (MQ=255)
                                                                             ggCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATg                                                               <  2:668232/140‑1 (MQ=255)
                                                                             ggCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATg                                                               <  1:513252/140‑1 (MQ=255)
                                                                                gcAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTTGGACGGGGGTG‑TTTATGGGGGCTGGCAAAACGATTAGCCTTT‑CCGGGGCGTCGATCATTTTCGTTTTTATGATCATTGGGTTTTTtctc                                                            >  2:1808346/1‑140 (MQ=255)
                                                                                 cAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGctct                                                           <  1:1015873/140‑1 (MQ=255)
                                                                                          aaaCCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGa                                                  <  1:64015/140‑1 (MQ=255)
                                                                                            aCCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATg                                                <  1:703069/140‑1 (MQ=255)
                                                                                              cGACATATTCAGCTTATTGCCATTGGCGGTGCCATTTGTACGGGGGTG‑TTTATGGGGGCTGGCAAAAAGATTAGCCTTG‑CCGGGCCGGCGATCATTTTCGTTTATATGATCATTTGTTTTATGCTCTTTTTTGTGATGCg                                              >  2:1719572/1‑140 (MQ=255)
                                                                                              cGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCg                                              >  1:329912/1‑140 (MQ=255)
                                                                                              cGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGGTG‑TTTATGGGGGCTGGCAAAACGATTAGCCTTG‑CCGGGCCGGCGATCATTTTCGTTTATATGATCATTTGTTTTTTTCTCTTTTTCGTGATGCg                                              >  2:734110/1‑140 (MQ=255)
                                                                                                 cATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGc                                           <  2:1416307/140‑1 (MQ=255)
                                                                                                    atTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAAt                                        <  1:507501/140‑1 (MQ=255)
                                                                                                             aTTGCCATTGGCGGTGCCATTGGTACGGGGGTG‑TTTATGGGGGCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGATCATTTTTGTTTATATGATCATTGGGTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGGAtt                               >  1:281931/1‑140 (MQ=255)
                                                                                                             aTTGCCATTGGCGGTGCCATTGGGACGGGGGTG‑TTTTTGGGGGCTGGCAAAACGATTAGCCTTG‑CCGGGGCGTCGATCATTTTTGTTTATATGATCATTGGTTTTTTGCTCTTTTTTGTGATGCGGGCAATGGGGGGAtt                               >  2:1337607/1‑140 (MQ=255)
                                                                                                              ttGCCATTGGCGGTGCCATTGGTACGGGGTTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATtg                              <  1:1274728/140‑1 (MQ=255)
                                                                                                               tGCCATTGGCGGTGCCATTGGTACGGGGTTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATtgc                             >  1:1990398/1‑140 (MQ=255)
                                                                                                               tGCCATTGGCGGTGCCATTGGTACGGGGGTG‑TTTTTGGGGGCTGGCAAAACGATTAGCCTTG‑CCGGGGCGTCGATCATTTTTGTTTATATGATTATTTGTTTTTTGCTCTTTTTCGTGATGCGGGCAATGGGGGGATtgt                             >  2:127783/1‑139 (MQ=255)
                                                                                                                  cATTGGCGGTGCCATTGGTACGGGGGTG‑TTTATTGGGGCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGATCATTTTTGTTTATATGATCATTGGTTTTTTGCTCTTTTTTGTGATGCGGGCAATGGGGGGATtgctgc                          >  2:131489/1‑140 (MQ=255)
                                                                                                                    ttGGCGGTGCCATTGGTACGGGGGTG‑TTTTTGGGGGCTGGCAAAACGATTAGCCTTT‑CCGGGGCGTCGGTCATTTTTGTTTTTATGATCATTGGTTTTTTTCTCTTTTTCGTGATGCGTGGAATGGGGGGATTTCTGCtt                        >  2:908806/1‑140 (MQ=255)
                                                                                                                     tGGCGGTGCCATTGGTACGGGGTTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCttt                       <  1:772165/140‑1 (MQ=255)
                                                                                                                      ggCGGTGGCCTTTGTACGGGGGTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCGGCGGTCATTTTCGTTTATATGATCATTGGTTTTTTGCTCTTTTTTGTGATGCGGGGAATGGGGGAATTGCTGCtttt                      >  2:393889/1‑139 (MQ=255)
                                                                                                                        cGGTGCCATTGGTACGGGGTTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGa                    <  2:1366857/140‑1 (MQ=255)
                                                                                                                           tGCCATTGGTACGGGGTTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATc                 <  2:1612022/140‑1 (MQ=255)
                                                                                                                            gCCATTGGTACGGGGGTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGGATTGCTGCTTTCGAATCt                >  2:1793939/1‑140 (MQ=255)
                                                                                                                             ccATTGGTACGGGGTTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTg               <  1:104772/140‑1 (MQ=255)
                                                                                                                              cATTGGTACGGGGTTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTgg              <  2:1062885/140‑1 (MQ=255)
                                                                                                                                tttggaCGGGGGTG‑TTTTTGGGGGCTGGCAAAACGATTAGCCTTT‑CCGGGCCGTCGGTCATTTTCGTTTATATGATCATTGGTTTTTTTCTCTTTTTTGTGATGCGGGCAATGGGGGGATTTCTGCTTTCGAATCTGGaa            >  2:543364/6‑140 (MQ=255)
                                                                                                                                 tGGTACGGGGTTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAAt           >  1:1591808/1‑140 (MQ=255)
                                                                                                                                   gTACGGGGTTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATAc         <  2:518437/140‑1 (MQ=255)
                                                                                                                                   gTACGGGGTTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATAc         <  1:191243/140‑1 (MQ=255)
                                                                                                                                      cGGGGTTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACaaa      >  2:414342/1‑140 (MQ=255)
                                                                                                                                       ggggTTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAAt     >  1:619115/1‑140 (MQ=255)
                                                                                                                                       ggggTTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAAt     <  2:696780/140‑1 (MQ=255)
                                                                                                                                       ggggTTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAAt     <  2:1990398/140‑1 (MQ=255)
                                                                                                                                          gttg‑ttTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCtt  >  1:1364884/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                            
GGTACAGGAAGAAAAAAACATGGTAGATCAGGTAAAAGTCGTTGCCGATGATCAGGCTCCGGCTGAACAGTCGCTACGGCGCAATCTCACAAACCGACATATTCAGCTTATTGCCATTGGCGGTGCCATTGGTACGGGGTTG‑TTTATGGGGTCTGGCAAAACGATTAGCCTTG‑CCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTT  >  NC_000913/4429845‑4430122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑