Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,968,302	A→G	F67S (TTC→TCC)	cheB ←	protein‑glutamate methylesterase/protein glutamine deamidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,968,302	0	A	G	100.0%	186.9 / NA	51	F67S (TTC→TCC)	cheB	protein‑glutamate methylesterase/protein glutamine deamidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/31);  total (20/31)

CCAGTTGCGGTTTGGTGACAAAATCTATCGCCCCCAGCTCCAGCGCGCGCAGCGTGACTTCTGACCCTTTGCCGGTCAGGGAAGAAACCATCACAACGGGCATTGGACGCAAACGCATTAATTTTTCGAGGAAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCACCATTTCCATGTCGCTATGGCTGTTGAT  >  NC_000913/1968171‑1968435                                                                                                                                    |                                                                                                                                      ccAGTTGCGGTTTGGTGACAAAATCTATCGCCCCCAGCTCCAGCGCGCGCAGCGTGACTTCTGACCCTTTGCCGGTCAGGGAAGAAACCATCACAACGGGCATTGGACGCAAACGCATTAATTTTTCGAGGGAATCCAgt                                                                                                                               <  1:292763/140‑1 (MQ=255) ccAGTTGCGGTTTGGTGACAAAATCTATCGCCCCCAGCTCCAGCGCGCGCAGCGTGACTTCTGACCCTTTGCCGGTCAGGGAAGAAACCATCACAACGGGCATTGGACGCAAACGCATTAATTTTTCGAGGGAATCCAgt                                                                                                                               <  2:370783/140‑1 (MQ=255)   aGTTGCGGTTTGGTGACAAAATCTATCGCCCCCAGCTCCAGCGCGCGCAGCGTGACTTCTGACCCTTTGCCGGTCAGGGAAGAAACCATCACAACGGGCATTGGACGCAAACGCATTAATTTTTCGAGGGAATCCAgtcc                                                                                                                             >  2:638772/1‑140 (MQ=255)     ttGCGGTTTGGTGACAAAATCTATCGCCCCCAGCTCCAGCGCGCGCAGCGTGACTTCTGACCCTTTGCCGGTCAGGGAAGAAACCATCACAACGGGCATTGGACGCAAACGCATTAATTTTTCGAGGGAATCCAgtccgt                                                                                                                           >  2:964079/1‑140 (MQ=255)      tGCGGTTTGGTGACAAAATCTATCGCCCCCAGCTCCAGCGCGCGCAGCGTGACTTCTGACCCTTTGCCGGTCAGGGAAGAAACCATCACAACGGGCATTGGACGCAAACGCATTAATTTTTCGAGGGAATCCAgtccgtc                                                                                                                          <  2:684082/140‑1 (MQ=255)            ttGGTGACAAAATCTATCGCCCCCAGCTCCAGCGCGCGCAGCGTGACTTCTGACCCTTTGCCGGTCAGGGAAGAAACCATCACAACGGGCATTGGACGCAAACGCATTAATTTTTCGAGGGAATCCAGTCCGTCCATCcg                                                                                                                    <  1:570942/140‑1 (MQ=255)                     aaaTCTATCGCCCCCAGCTCCAGCGCGCGCAGCGTGACTTCTGACCCTTTGCCGGTCAGGGAAGAAACCATCACAACGGGCATTGGACGCAAACGCATTAATTTTTCGAGGGAATCCAGTCCGTCCATCCGCGGCATTTc                                                                                                           <  2:237086/140‑1 (MQ=255)                       aTCTATCGCCCCCAGCTCCAGCGCGCGCAGCGTGACTTCTGACCCTTTGCCGGTCAGGGAAGAAACCATCACAACGGGCATTGGACGCAAACGCATTAATTTTTCGAGGGAATCCAGTCCGTCCATCCGCGGCATTTCaa                                                                                                         <  2:113142/140‑1 (MQ=255)                         cTATCGCCCCCAGCTCCAGCGCGCGCAGCGTGACTTCTGACCCTTTGCCGGTCAGGGAAGAAACCATCACAACGGGCATTGGACGCAAACGCATTAATTTTTCGAGGGAATCCAGTCCGTCCATCCGCGGCATTTCAACa                                                                                                       >  1:167627/1‑140 (MQ=255)                         cTATCGCCCCCAGCTCCAGCGCGCGCAGCGTGACTTCTGACCCTTTGCCGGTCAGGGAAGAAACCATCACAACGGGCATTGGACGCAAACGCATTAATTTTTCGAGGGAATCCAGTCCGTCCATCCGCGGCATTTCAACa                                                                                                       >  2:19512/1‑140 (MQ=255)                            tCGCCCCCAGCTCCAGCGCGCGCAGCGTGACTTCTGACCCTTTGCCGGTCAGGGAAGAAACCATCACAACGGGCATTGGACGCAAACGCATTAATTTTTCGAGGGAATCCAGTCCGTCCATCCGCGGCATTTCAACATcc                                                                                                    <  2:97004/140‑1 (MQ=255)                            tCGCCCCCAGCTCCAGCGCGCGCAGCGTGACTTCTGACCCTTTGCCGGTCAGGGAAGAAACCATCACAACGGGCATTGGACGCAAACGCATTAATTTTTCGAGGGAATCCAGTCCGTCCATCCGCGGCATTTCAACATcc                                                                                                    >  1:617829/1‑140 (MQ=255)                                  ccAGCTCCAGCGCGCGCAGCGTGACTTCTGACCCTTTGCCGGTCAGGGAAGAAACCATCACAACGGGCATTGGACGCAAACGCATTAATTTTTCGAGGGAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTc                                                                                              >  1:659404/1‑140 (MQ=255)                                    aGCTCCAGCGCGCGCAGCGTGACTTCTGACCCTTTGCCGGTCAGGGAAGAAACCATCACAACGGGCATTGGACGCAAACGCATTAATTTTTCGAGGGAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAg                                                                                            <  1:893839/140‑1 (MQ=255)                                    aGCTCCAGCGCGCGCAGCGTGACTTCTGACCCTTTGCCGGTCAGGGAAGAAACCATCACAACGGGCATTGGACGCAAACGCATTAATTTTTCGAGGGAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAg                                                                                            <  1:553039/140‑1 (MQ=255)                                      cTCCAGCGCGCGCAGCGTGACTTCTGACCCTTTGCCGGTCAGGGAAGAAACCATCACAACGGGCATTGGACGCAAACGCATTAATTTTTCGAGGGAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGca                                                                                          <  2:30461/140‑1 (MQ=255)                                         cAGCGCGCGCAGCGTGACTTCTGACCCTTTGCCGGTCAGGGAAGAAACCATCACAACGGGCATTGGACGCAAACGCATTAATTTTTCGAGGGAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACAt                                                                                       <  2:356926/140‑1 (MQ=255)                                              cgcgcAGCGTGACTTCTGACCCTTTGCCGGTCAGGGAAGAAACCATCACAACGGGCATTGGACGCAAACGCATTAATTTTTCGAGGGAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGa                                                                                  >  2:696602/1‑140 (MQ=255)                                                cgcAGCGTGACTTCTGACCCTTTGCCGGTCAGGGAAGAAACCATCACAACGGGCATTGGACGCAAACGCATTAATTTTTCGAGGGAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGAtt                                                                                >  2:307314/1‑140 (MQ=255)                                                            tCTGACCCTTTGCCGGTCAGGGAAGAAACCATCACAACGGGCATTGGACGCAAACGCATTAATTTTTCGAGGGAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAAt                                                                    <  2:168121/140‑1 (MQ=255)                                                            ccTGACCCTTTGCCGGTCAGGGAAGAAACCATCACAACGGGCATTGGACGCAAACGCATTAATTTTTCGAGGGAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAAt                                                                    <  1:842037/139‑1 (MQ=255)                                                               gACCCTTTGCCGGTCAGGGAAGAAACCATCACAACGGGCATTGGACGCAAACGCATTAATTTTTCGAGGGAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCaa                                                                 >  1:750408/1‑140 (MQ=255)                                                                    tttGCCGGTCAGGGAAGAAACCATCACAACGGGCATTGGACGCAAACGCATTAATTTTTCGAGGGAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCAc                                                            >  1:601169/1‑140 (MQ=255)                                                                      tGCCGGTCAGGGAAGAAACCATCACAACGGGCATTGGACGCAAACGCATTAATTTTTCGAGGGAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCAcgc                                                          <  2:896609/140‑1 (MQ=255)                                                                      tGCCGGTCAGGGAAGAAACCATCACAACGGGCATTGGACGCAAACGCATTAATTTTTCGAGGGAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCAcgc                                                          <  1:964079/140‑1 (MQ=255)                                                                       gCCGGTCAGGGAAGAAACCATCACAACGGGCATTGGACGCAAACGCATTAATTTTTCGAGGGAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCAcgcg                                                         >  2:1021034/1‑140 (MQ=255)                                                                              aGGGAAGAAACCATCACAACGGGCATTGGACGCAAACGCATTAATTTTTCGAGGGAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAg                                                  >  1:400753/1‑140 (MQ=255)                                                                              aGGGAAGAAACCATCACAACGGGCATTGGACGCAAACGCATTAATTTTTCGAGGGAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAg                                                  <  1:971586/140‑1 (MQ=255)                                                                                ggAAGAAACCATCACAACGGGCATTGGACGCAAACGCATTAATTTTTCGAGGGAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCg                                                >  2:308874/1‑140 (MQ=255)                                                                                ggAAGAAACCATCACAACGGGCATTGGACGCAAACGCATTAATTTTTCGAGGGAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCg                                                >  2:574351/1‑140 (MQ=255)                                                                                     aaaCCATCACAACGGGCATTGGACGCAAACGCATTAATTTTTCGAGGGAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCa                                           >  1:904765/1‑140 (MQ=255)                                                                                          aTCACAACGGGCATTGGACGCAAACGCATTAATTTTTCGAGGGAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGgcgc                                      >  2:147561/1‑140 (MQ=255)                                                                                          aTCACAACGGGCATTGGACGCAAACGCATTAATTTTTCGAGGGAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGgcgc                                      >  2:429536/1‑140 (MQ=255)                                                                                          aTCACAACGGGCATTGGACGCAAACGCATTAATTTTTCGAGGGAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGgcgc                                      >  2:436565/1‑140 (MQ=255)                                                                                             acaACGGGCATTGGACGCAAACGCATTAATTTTTCGAGGGAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGt                                   <  2:750408/140‑1 (MQ=255)                                                                                              caACGGGCATTGGACGCAAACGCATTAATTTTTCGAGGGAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTc                                  <  2:850589/140‑1 (MQ=255)                                                                                                 cGGGCATTGGACGCAAACGCATTAATTTTTCGAGGGAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGcc                               <  2:443094/140‑1 (MQ=255)                                                                                                        tGGACGCAAACGCATTAATTTTTCGAGGGAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCACCAttt                        >  2:87942/1‑140 (MQ=255)                                                                                                            cGCAAACGCATTAATTTTTCGAGGGAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCACCATTTCCAt                    <  1:91824/140‑1 (MQ=255)                                                                                                            cGCAAACGCATTAATTTTTCGAGGGAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCACCATTTCCAt                    <  1:696602/140‑1 (MQ=255)                                                                                                                  cGCATTAATTTTTCGAGGGAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCACCATTTCCATGTCGCt              <  2:246817/140‑1 (MQ=255)                                                                                                                  cGCATTAATTTTTCGAGGGAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCACCATTTCCATGTCGCt              <  1:621307/140‑1 (MQ=255)                                                                                                                    cATTAATTTTTCGAGGGAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCACCATTTCCATGTCGCTAt            <  1:19512/140‑1 (MQ=255)                                                                                                                    cATTAATTTTTCGAGGGAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCACCATTTCCATGTCGCTAt            <  1:308874/140‑1 (MQ=255)                                                                                                                    cATTAATTTTTCGAGGGAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCACCATTTCCATGTCGCTAt            <  1:781529/140‑1 (MQ=255)                                                                                                                      ttAATTTTTCGAGGGAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCACCATTTCCATGTCGCTATgg          <  1:1021034/140‑1 (MQ=255)                                                                                                                         aTTTTTCGAGGGAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCACCATTTCCATGTCGCTATGGCTg       <  2:617829/140‑1 (MQ=255)                                                                                                                         aTTTTTCGAGGGAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCACCATTTCCATGTCGCTATGGCTg       <  1:355820/140‑1 (MQ=255)                                                                                                                         aTTTTTCGAGGGAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCACCATTTCCATGTCGCTATGGCTg       >  2:782811/1‑140 (MQ=255)                                                                                                                         aTTTTTCGAGGGAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCACCATTTCCATGTCGCTATGGCTg       <  1:380558/140‑1 (MQ=255)                                                                                                                         aTTTTTCGAGGGAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCACCATTTCCATGTCGCTATGGCTg       <  1:429536/140‑1 (MQ=255)                                                                                                                              tCGAGGGAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCACCATTTCCATGTCGCTATGGCTGTtgat  <  1:307314/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                      CCAGTTGCGGTTTGGTGACAAAATCTATCGCCCCCAGCTCCAGCGCGCGCAGCGTGACTTCTGACCCTTTGCCGGTCAGGGAAGAAACCATCACAACGGGCATTGGACGCAAACGCATTAATTTTTCGAGGAAATCCAGTCCGTCCATCCGCGGCATTTCAACATCCAGCGTCAGCACATCGGGATTGAATTTCTTAATCAAGTCACGCGCGACCAGCGGATCAGGCGCGGTCGCCACCATTTCCATGTCGCTATGGCTGTTGAT  >  NC_000913/1968171‑1968435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑