Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,029,025	G→A	T636M (ACG→ATG)	ygfT ←	fused putative oxidoreductase, Fe‑S subunit and nucleotide‑binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,029,025	0	G	A	100.0%	247.4 / NA	69	T636M (ACG→ATG)	ygfT	fused putative oxidoreductase, Fe‑S subunit and nucleotide‑binding subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (39/30);  total (39/30)

TTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCGTATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCC  >  NC_000913/3028888‑3029158                                                                                                                                          |                                                                                                                                      ttCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGCGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATa                                                                                                                                     >  2:643780/1‑140 (MQ=255)     gCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCaa                                                                                                                                 >  1:714952/1‑140 (MQ=255)     gCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCaa                                                                                                                                 <  1:561141/140‑1 (MQ=255)     gCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCaa                                                                                                                                 >  1:156905/1‑140 (MQ=255)      ccGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCaaa                                                                                                                                <  1:593611/140‑1 (MQ=255)       cGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCAAAc                                                                                                                               <  2:708257/140‑1 (MQ=255)       cGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCAAAc                                                                                                                               <  2:747192/140‑1 (MQ=255)       cGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCAAAc                                                                                                                               <  1:652558/140‑1 (MQ=255)       cGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCAAAc                                                                                                                               <  2:895775/140‑1 (MQ=255)         gggTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCAAACag                                                                                                                             <  1:1025470/140‑1 (MQ=255)         gggTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCAAACag                                                                                                                             >  1:769014/1‑140 (MQ=255)          ggTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCAAACaga                                                                                                                            >  1:385885/1‑140 (MQ=255)                     aTCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATc                                                                                                                 >  1:569696/1‑140 (MQ=255)                        ggCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATcgcg                                                                                                              <  2:629723/140‑1 (MQ=255)                        ggCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATcgcg                                                                                                              <  1:720819/140‑1 (MQ=255)                            ttCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGcgcc                                                                                                          <  1:986998/140‑1 (MQ=255)                                    cTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCgcctgcct                                                                                                  >  1:38691/1‑140 (MQ=255)                                         cTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAACACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTg                                                                                             >  1:205547/1‑140 (MQ=255)                                            aaGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCgg                                                                                          >  2:1035218/1‑140 (MQ=255)                                              gggTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGcc                                                                                        >  2:645761/1‑140 (MQ=255)                                              gggTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGcc                                                                                        <  1:253297/140‑1 (MQ=255)                                                   aGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGc                                                                                   >  2:538851/1‑140 (MQ=255)                                                     cGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAg                                                                                 <  2:769014/140‑1 (MQ=255)                                                       aCCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTg                                                                               <  2:475680/140‑1 (MQ=255)                                                       aCCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTg                                                                               <  2:714952/140‑1 (MQ=255)                                                         cacacaGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGAc                                                                             >  1:147658/1‑140 (MQ=255)                                                         cacacaGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGAc                                                                             >  2:179266/1‑140 (MQ=255)                                                         cacacaGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATAGTGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGAc                                                                             >  1:768306/1‑140 (MQ=255)                                                              aGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCa                                                                        <  1:643780/140‑1 (MQ=255)                                                              aGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCa                                                                        >  2:157731/1‑140 (MQ=255)                                                                  tACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATc                                                                    >  2:681067/1‑140 (MQ=255)                                                                   aCTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATcc                                                                   >  1:542977/1‑140 (MQ=255)                                                                   aCTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATcc                                                                   <  1:645761/140‑1 (MQ=255)                                                                      tCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCcgcg                                                                >  2:455471/1‑140 (MQ=255)                                                                      tCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCcgcg                                                                <  2:569696/140‑1 (MQ=255)                                                                          aaGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCAt                                                            >  2:886075/1‑140 (MQ=255)                                                                           aGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATg                                                           <  2:637277/140‑1 (MQ=255)                                                                               cGGCAGCCAATACAGTCAGCCGGATTAACGATAATTACCGATTTCATCACGATGCCTTCATATCAAATAGAGTTAACATATCGCGCGCCGCCTGCCTCCCTGCGGAAATTGCAGTGACAACCAGATCCGCGCCATGaatt                                                       <  2:711543/140‑3 (MQ=255)                                                                               cGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACt                                                       >  2:1064374/1‑140 (MQ=255)                                                                                  cAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCa                                                    <  1:1051149/140‑1 (MQ=255)                                                                                     ccAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATca                                                 >  2:792685/1‑140 (MQ=255)                                                                                         tACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATcaccac                                             >  1:827945/1‑140 (MQ=255)                                                                                              tCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCa                                        >  1:573033/1‑140 (MQ=255)                                                                                                aGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCaaa                                      >  2:702205/1‑140 (MQ=255)                                                                                                 gCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAg                                     >  1:10643/1‑140 (MQ=255)                                                                                                 gCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAg                                     >  2:465463/1‑140 (MQ=255)                                                                                                  ccGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGa                                    >  1:134484/1‑140 (MQ=255)                                                                                                  ccGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGa                                    <  1:886075/140‑1 (MQ=255)                                                                                                   cGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCa                                                    >  2:356983/1‑123 (MQ=255)                                                                                                   cGGATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCa                                                    <  1:356983/123‑1 (MQ=255)                                                                                                     gATTAACGATAATTAACGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACtt                                 >  2:599689/1‑140 (MQ=255)                                                                                                        tAACGATAATTAACGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACttttt                              >  1:456049/1‑140 (MQ=255)                                                                                                            gATAATTAACGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATTAACTGCATCACCACCAGCAAAGACTTTTTTCAg                          >  1:663670/1‑140 (MQ=255)                                                                                                            gATAATTAACGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAg                          >  2:900493/1‑140 (MQ=255)                                                                                                             aTAATTAACGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGa                         <  1:1035218/140‑1 (MQ=255)                                                                                                             aTAATTAACGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCCCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGa                         <  2:227264/140‑1 (MQ=255)                                                                                                                aTTAACGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGc                      >  2:304657/1‑140 (MQ=255)                                                                                                                aTTAACGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGc                      >  1:233025/1‑140 (MQ=255)                                                                                                                aTTAACGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGc                      >  2:708995/1‑140 (MQ=255)                                                                                                                   aaCGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTc                   <  2:596395/140‑1 (MQ=255)                                                                                                                     cGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTg                 >  2:1026059/1‑140 (MQ=255)                                                                                                                     cGATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTg                 <  1:1026059/140‑1 (MQ=255)                                                                                                                      gATTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTgg                >  2:1021693/1‑140 (MQ=255)                                                                                                                       aTTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTggg               <  1:681067/140‑1 (MQ=255)                                                                                                                       aTTTCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTggg               <  1:1064374/140‑1 (MQ=255)                                                                                                                        tttCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGggt              <  2:156905/140‑1 (MQ=255)                                                                                                                        tttCATCACGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGggt              >  1:232131/1‑140 (MQ=255)                                                                                                                              caCGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTaa        >  2:73671/1‑140 (MQ=255)                                                                                                                                cGATGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAAt      >  2:861028/1‑140 (MQ=255)                                                                                                                                  aTGCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATAc    <  2:134484/140‑1 (MQ=255)                                                                                                                                    gCCTTCATATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATAccc  <  2:232131/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      TTCAGCCGGGGTAAAAAGACATCGGCATTCAACTCCTGTTCTGAAGGGTGAGCGACCACACAGGCTACTTCACAAGTACGGCAGCCAATACAGTCAGCCGGATTAACGATAATTAACGATTTCATCACGATGCCTTCGTATCAAACAGAGTTAACATATCGCGCGCCGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCC  >  NC_000913/3028888‑3029158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑