BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,969,316	G→T	intergenic (‑339/+14)	rppH ← / ← ygdT	RNA pyrophosphohydrolase/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,969,316	0	G	T	99.3%	491.5 / ‑5.4	137	intergenic (‑339/+14)	rppH/ygdT	RNA pyrophosphohydrolase/hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (80/56); total (81/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 137 total aligned reads displayed.

ACTACGGGAAAACCCGGAGCATTCTGAATAAGCCCGATATAGACATTTTAAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACGGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACTGGAGTTTCGTCGCACGTCAAGGCTGTAAATGGAAACAGTAGTGGAGGTTTTTCACAGT  >  NC_000913/2969180‑2969451

actacGGGAAAACCCGGAGCATTCTGAATAAGCCCGATATAGACATTTTAAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGt                                                                                                                                      <  1:1482366/140‑1 (MQ=255)

 ctacGGGAAAACCCGGAGCATTCTGAATAAGCCCGATATAGACATTTTAAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGtt                                                                                                                                     <  1:1494393/140‑1 (MQ=255)

     gggAAAACCCGGAGCATTCTGAATAAGCCCGATATAGACATTTTAAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCg                                                                                                                                 >  1:516828/1‑140 (MQ=255)

     gggAAAACCCGGAGCATTCTGAATAAGCCCGATATAGACATTTTAAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCg                                                                                                                                 <  2:1182379/140‑1 (MQ=255)

      ggAAAACCCGGAGCATTCTGAATAAGCCCGATATAGACATTTTAAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGa                                                                                                                                >  1:322230/1‑140 (MQ=255)

      ggAAAACCCGGAGCATTCTGAATAAGCCCGATATAGACATTTTAAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGa                                                                                                                                <  1:1817443/140‑1 (MQ=255)

      ggAAAACCCGGAGCATTCTGAATAAGCCCGATATAGACATTTTAAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGa                                                                                                                                >  2:1946095/1‑140 (MQ=255)

      ggAAAACCCGGAGCATTCTGAATAAGCCCGATATAGACATTTTAAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACATCTCATTTACTGGTTGGCGa                                                                                                                                >  2:850165/1‑140 (MQ=255)

      ggAAAACCCGGAGCATTCTGAATAAGCCCGATATAGACATTTTAAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCATGACCCAGGTCACACCTCTCATTTACTGGTTGGCGa                                                                                                                                >  2:1362069/1‑140 (MQ=255)

                     ttCTGAATAAGCCCGATATAGACATTTTAAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGc                                                                                                                 >  1:1508659/1‑140 (MQ=255)

                      tCTGAATAAGCCCGATATAGACATTTTAAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGcc                                                                                                                >  2:210087/1‑140 (MQ=255)

                       cTGAATAAGCCCGATATAGACATTTTAAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCg                                                                                                               <  1:417152/140‑1 (MQ=255)

                       cTGAATAAGCCCGATATAGACATTTTAAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCg                                                                                                               <  1:601510/140‑1 (MQ=255)

                       cTGAATAAGCCCGATATAGACATTTTAAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCg                                                                                                               <  1:894069/140‑1 (MQ=255)

                       cTGAATAAGCCCGATATAGACATTTTAAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCg                                                                                                               >  1:1799876/1‑140 (MQ=255)

                             aaGCCCGATATAGACATTTTAAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTa                                                                                                         <  2:1131164/140‑1 (MQ=255)

                               gCCCGATATAGACATTTTAAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATc                                                                                                       >  2:1389971/1‑140 (MQ=255)

                                cccGATATAGACATTTTAAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATcc                                                                                                      >  2:1472371/1‑140 (MQ=255)

                                 ccGATATAGACATTTTAAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCca                                                                                                     <  2:1576237/140‑1 (MQ=255)

                                    atatAGACATTTTAAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAg                                                                                                  >  2:1805953/1‑140 (MQ=255)

                                    atatAGACATTTTAAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAg                                                                                                  >  2:340537/1‑140 (MQ=255)

                                       taGACATTTTAAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAAt                                                                                               <  2:1383283/140‑1 (MQ=255)

                                        aGACATTTTAAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAAtg                                                                                              <  2:1289658/140‑1 (MQ=255)

                                        aGACATTTTAAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAAtg                                                                                              <  1:1570818/140‑1 (MQ=255)

                                          aCATTTTAAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGACCAACCAATGCCGTATTTATCCACAGAAtgtg                                                                                            <  2:672482/140‑1 (MQ=255)

                                             ttttAAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCa                                                                                         <  2:1027360/140‑1 (MQ=255)

                                              tttAAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCAc                                                                                        >  2:613970/1‑140 (MQ=255)

                                              tttAAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCAc                                                                                        >  1:953889/1‑140 (MQ=255)

                                               ttAAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACt                                                                                       >  2:586991/1‑140 (MQ=255)

                                               ttAAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACt                                                                                       <  2:268300/140‑1 (MQ=255)

                                               ttAAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACt                                                                                       <  1:578094/140‑1 (MQ=255)

                                               ttAAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACt                                                                                       >  2:1728521/1‑140 (MQ=255)

                                               ttAAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACt                                                                                       <  2:979846/140‑1 (MQ=255)

                                               ttAAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACt                                                                                       <  1:1262639/140‑1 (MQ=255)

                                               ttAAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACt                                                                                       >  1:1259710/1‑140 (MQ=255)

                                               ttAAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACt                                                                                       >  1:1044511/1‑140 (MQ=255)

                                               ttAAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGGGCCACt                                                                                       >  1:831465/1‑140 (MQ=255)

                                               ttAAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGCCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACt                                                                                       >  2:356561/1‑140 (MQ=255)

                                                      atTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTaa                                                                                >  1:1678673/1‑140 (MQ=255)

                                                        tCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGc                                                                              >  1:99079/1‑140 (MQ=255)

                                                        tCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGc                                                                              >  2:1948511/1‑140 (MQ=255)

                                                         cATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCa                                                                             <  2:380795/140‑1 (MQ=255)

                                                          aTTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCAc                                                                            >  2:185067/1‑140 (MQ=255)

                                                            tATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTg                                                                          <  1:210087/140‑1 (MQ=255)

                                                              tCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGTACTGaa                                                                        <  2:60494/140‑1 (MQ=255)

                                                              tCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGaa                                                                        <  1:50825/140‑1 (MQ=255)

                                                              tCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGaa                                                                        <  2:1179266/140‑1 (MQ=255)

                                                               cAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGGATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAAc                                                                       >  1:539465/1‑140 (MQ=255)

                                                                 gTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCa                                                                     >  2:369988/1‑140 (MQ=255)

                                                                    aaTGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTa                                                                  <  1:1362069/140‑1 (MQ=255)

                                                                      tGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTaaa                                                                >  2:865108/1‑140 (MQ=255)

                                                                      tGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTaaa                                                                >  2:864326/1‑140 (MQ=255)

                                                                      tGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTaaa                                                                >  2:1046449/1‑140 (MQ=255)

                                                                      tGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTaaa                                                                >  1:166847/1‑140 (MQ=255)

                                                                          ttACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACt                                                            >  1:1627937/1‑140 (MQ=255)

                                                                           tACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACTg                                                           >  1:1385046/1‑140 (MQ=255)

                                                                            aCATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACTgg                                                          >  1:1008436/1‑140 (MQ=255)

                                                                              aTGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACTGGAg                                                        <  1:1046449/140‑1 (MQ=255)

                                                                                gTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACTGGAGtt                                                      >  2:1143695/1‑140 (MQ=255)

                                                                                gTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACTGGAGtt                                                      >  1:1123904/1‑140 (MQ=255)

                                                                                gTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACTGGAGtt                                                      >  1:94029/1‑140 (MQ=255)

                                                                                  aaTTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACTGGAGTTtc                                                    >  2:354396/1‑140 (MQ=255)

                                                                                  aaTTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACTGGAGTTtc                                                    >  2:730930/1‑140 (MQ=255)

                                                                                     tttAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACTGGAGTTtcgtc                                                 >  2:732796/1‑140 (MQ=255)

                                                                                      ttAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACTGGAGTTtcgtcg                                                <  2:562817/140‑1 (MQ=255)

                                                                                      ttAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACTGGAGTTtcgtcg                                                <  1:1227371/140‑1 (MQ=255)

                                                                                      ttAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACTGGAGTTtcgtcg                                                <  1:692786/140‑1 (MQ=255)

                                                                                        aGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACTGGAGTTTCGTCGCa                                              >  1:724762/1‑140 (MQ=255)

                                                                                        aGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACTGGAGTTTCGTCGCa                                              >  1:718657/1‑140 (MQ=255)

                                                                                         gTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACTGGAGTTTCGTCGCAc                                             >  2:12983/1‑140 (MQ=255)

                                                                                            aTGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACTGGAGTTTCGTCGCACGTc                                          >  2:674108/1‑140 (MQ=255)

                                                                                             tGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACTGGAGTTTCGTCGCACGTCa                                         <  1:193778/140‑1 (MQ=255)

                                                                                               aaaaaGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACTGGAGTTTCGTCGCACGTCAAg                                       >  1:1147272/1‑140 (MQ=255)

                                                                                                   agagTAATTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACTGGAGTTTCGTCGCACGTCAAGGCTg                                   <  1:1805953/140‑1 (MQ=255)

                                                                                                        aaTTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACTGGAGTTTCGTCGCACGTCAAGGCTGTAAAt                              <  2:1008436/140‑1 (MQ=255)

                                                                                                        aaTTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACTGGAGTTTCGTCGCACGTCAAGGCTGTAAAt                              >  2:638645/1‑140 (MQ=255)

                                                                                                        aaTTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACTGGAGTTTCGTCGCACGTCAAGGCTGTAAAt                              <  1:732796/140‑1 (MQ=255)

                                                                                                         aTTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACTGGAGTTTCGTCGCACGTCa                                         >  2:1728159/1‑128 (MQ=255)

                                                                                                         aTTCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACTGGAGTTTCGTCGCACGTCAAGGCTGTAAATg                             >  2:251824/1‑140 (MQ=255)

                                                                                                           tCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACTGGAGTTTCGTCGCACGTCAAGGCTGTAAATGGa                           <  1:185067/140‑1 (MQ=255)

                                                                                                           tCGTGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACTGGAGTTTCGTCGCACGTCAAGGCTGTAAATGGa                           <  2:1144283/140‑1 (MQ=255)

                                                                                                              tGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACTGGAGTTTCGTCGCACGTCAAGGCTGTAAATGGAAAc                        <  1:1161136/140‑1 (MQ=255)

                                                                                                              tGACCCAGGTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACTGGAGTTTCGTCGCACGTCAAGGCTGTAAATGGAAAc                        <  2:715558/140‑1 (MQ=255)

                                                                                                                     ggTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACTGGAGTTTCGTCGCACGTCAAGGCTGTAAATGGAAACAGTAGTg                 <  2:1259710/140‑1 (MQ=255)

                                                                                                                     ggTCACACCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACTGGAGTTTCGTCGCACGTCAAGGCTGTAAATGGAAACAGTAGTg                 >  1:1424364/1‑140 (MQ=255)

                                                                                                                        cacaCCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACTGGAGTTTCGTCGCACGTCAAGGCTGTAAATGGAAACAGTAGTGGAg              <  2:1799876/140‑1 (MQ=255)

                                                                                                                        cacaCCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACTGGAGTTTCGTCGCACGTCAAGGCTGTAAATGGAAACAGTAGTGGAg              <  1:1406972/140‑1 (MQ=255)

                                                                                                                         acacCTCTCATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACTGGAGTTTCGTCGCACGTCAAGGCTGTAAATGGAAACAGTAGTGGAgg             <  1:2040447/140‑1 (MQ=255)

                                                                                                                              tctcATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACTGGAGTTTCGTCGCACGTCAAGGCTGTAAATGGAAACAGTAGTGGAGGttttt        >  1:750702/1‑140 (MQ=255)

                                                                                                                              tctcATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACTGGAGTTTCGTCGCACGTCAAGGCTGTAAATGGAAACAGTAGTGGAGGttttt        <  1:1989372/140‑1 (MQ=255)

                                                                                                                               ctcATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACTGGAGTTTCGTCGCACGTCAAGGCTGTAAATGGAAACAGTAGTGGAGGTTTTTc       >  1:547403/1‑140 (MQ=255)

                                                                                                                               ctcATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACTGGAGTTTCGTCGCACGTCAAGGCTGTAAATGGAAACAGTAGTGGAGGTTTTTc       >  1:132732/1‑140 (MQ=255)

                                                                                                                                tcATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACTGGAGTTTCGTCGCACGTCAAGGCTGTAAATGGAAACAGTAGTgg                <  2:626743/130‑1 (MQ=255)

                                                                                                                                tcATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACTGGAGTTTCGTCGCACGTCAAGGCTGTAAATGGAAACAGTAGTgg                >  1:626743/1‑130 (MQ=255)

                                                                                                                                tcATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACTGGAGTTTCGTCGCACGTCAAGGCTGTAAATGGAAACAGTAGTGGAGGTTTTTca      >  2:353679/1‑140 (MQ=255)

                                                                                                                                tcATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACTGGAGTTTCGTCGCACGTCAAGGCTGTAAATGGAAACAGTAGTGGAGGTTTTTca      >  2:2001530/1‑140 (MQ=255)

                                                                                                                                tcATTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACTGGAGTTTCGTCGCACGTCAAGGCTGTAAATGGAAACAGTAGTGGAGGTTTTTca      >  1:170361/1‑140 (MQ=255)

                                                                                                                                  aTTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACTGGAGTTTCGTCGCACGTCAAGGCTGTAAATGGAAACAGTAGTGGAGGTTTTTcaca    >  2:274807/1‑140 (MQ=255)

                                                                                                                                  aTTTACTGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACTGGAGTTTCGTCGCACGTCAAGGCTGTAAATGGAAACAGTAGTGGAGGTTTTTcaca    <  2:1461747/140‑1 (MQ=255)

                                                                                                                                  aTTTACGGGTTGGCGAAAGATCAACCAATGCCTTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACTGGCGTTTCGTCGCACGTCAAGGCTGTAAATGGAAACAGTAGTGGAGGTTTTTcaca    >  2:946683/1‑140 (MQ=255)

ACTACGGGAAAACCCGGAGCATTCTGAATAAGCCCGATATAGACATTTTAAAATATTCATTATCAGTCAATGCATTACATGTAATTTTAGTAATGAAAAAGAGTAATTCGTGACCCAGGTCACACCTCTCATTTACGGGTTGGCGAAAGATCAACCAATGCCGTATTTATCCACAGAATGTGCCACTAAGTTAAGCACTGAACCACTAAAAACTGGAGTTTCGTCGCACGTCAAGGCTGTAAATGGAAACAGTAGTGGAGGTTTTTCACAGT  >  NC_000913/2969180‑2969451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`