Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,604,3440CT51.7% 33.0 / 120.7 89S62F (TCT→TTT) bglJDNA‑binding transcriptional regulator BglJ
Reads supporting (aligned to +/- strand):  ref base C (8/35);  new base T (46/0);  total (54/35)
Fisher's exact test for biased strand distribution p-value = 2.10e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.85e-12
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 106 total aligned reads displayed.

ATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAA‑CT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAT  >  NC_000913/4604207‑4604481
                                                                                                                                          |                                                                                                                                           
aTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑Ttttttttt                                                                                                                                           >  2:915569/1‑140 (MQ=255)
aTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑Ttttttctt                                                                                                                                           >  1:232928/1‑140 (MQ=255)
   cGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑Ttttttcttttt                                                                                                                                        <  1:299469/140‑1 (MQ=255)
   cGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑Ttttttcttttt                                                                                                                                        <  1:1191578/140‑1 (MQ=255)
   cGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑Ttttttcttttt                                                                                                                                        <  2:64494/140‑1 (MQ=255)
     tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTttttctttttct                                                                                                                                      <  1:737026/140‑1 (MQ=255)
     tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTttttctttttct                                                                                                                                      <  1:204824/140‑1 (MQ=255)
     tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTttttctttttct                                                                                                                                      <  2:160496/140‑1 (MQ=255)
      cATGAGTAGTATCGGGATTGAGAGCTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACATATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑Atttttttttttgtt                                                                                                                                     >  2:268649/1‑137 (MQ=255)
       aggAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGc                                                                                                                                    <  1:1569859/138‑1 (MQ=255)
       aTGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGc                                                                                                                                    <  2:431078/140‑1 (MQ=255)
              gtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATgaga                                                                                                                             <  1:71036/140‑1 (MQ=255)
                   ggTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTTCCATGAGAAGTGa                                                                                                                        >  2:1137967/1‑140 (MQ=255)
                    gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCGGGGGTCATTTCAGGATGCAATGTCGCGGATCTCGTTTGCGGGGGTCC‑TTTTTTTTTTTTTTTCCCTGGGAAggtgg                                                                                                                       >  2:31207/1‑136 (MQ=255)
                    gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCC‑TTTTTTCTTTTTTTTCCCTGAGaaaggag                                                                                                                       >  1:1540687/1‑135 (MQ=255)
                    gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTTCCCTGGGAAGGGAg                                                                                                                       >  2:1109939/1‑140 (MQ=255)
                    gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTGCCCTGAGaaaagag                                                                                                                       >  2:478708/1‑135 (MQ=255)
                    gTATTGAGAGTTTATTCAGAAAGTTTGCGGGGAACCATTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAg                                                                                                                       >  1:1363803/1‑140 (MQ=255)
                      aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAgcg                                                                                                                     <  2:1484494/140‑1 (MQ=255)
                      aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAgcg                                                                                                                     <  1:1137967/140‑1 (MQ=255)
                        tGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTTCCCTGAGAAGTGAGGGCa                                                                                                                   >  2:1401603/1‑140 (MQ=255)
                        tGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTCTTCCCTGAGGAGTGAgcgcg                                                                                                                   >  2:1248417/1‑139 (MQ=255)
                        tGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTTTGCCCTGAGAAGTGAGGGCa                                                                                                                   >  1:11249/1‑140 (MQ=255)
                         gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTGCCATGAGAAAGGAgcgcgg                                                                                                                  >  2:966429/1‑138 (MQ=255)
                         gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCag                                                                                                                  >  1:651793/1‑140 (MQ=255)
                         gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGAATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCag                                                                                                                  <  1:305189/140‑1 (MQ=255)
                          agagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCaga                                                                                                                 <  1:402635/140‑1 (MQ=255)
                          agagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCaga                                                                                                                 <  1:1438042/140‑1 (MQ=255)
                          agagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCaga                                                                                                                 <  1:332883/140‑1 (MQ=255)
                           gagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCACagag                                                                                                                <  1:1436019/140‑1 (MQ=255)
                            agTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTGCCCTGAGAAGTGAGCGCagagg                                                                                                               >  1:1510441/1‑139 (MQ=255)
                              tttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTTCCATTAGAAGAGGGCGCCGAGAgg                                                                                                             >  1:379222/1‑140 (MQ=255)
                               ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAggg                                                                                                            <  2:232928/140‑1 (MQ=255)
                               ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAggg                                                                                                            <  1:72477/140‑1 (MQ=255)
                               ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAggg                                                                                                            <  1:38600/140‑1 (MQ=255)
                                 aTTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTTCCCTGAGAAGGGGGCGCagggaggggt                                                                                                          >  2:915484/1‑138 (MQ=255)
                                       aaaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCtt                                                                                                    >  1:818170/1‑140 (MQ=255)
                                        aaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCTGCGGTCA‑TTTTTTTTTTTTTTTTCCTGAGAAGTGGGCGCAGAGAGGGGTTATCttt                                                                                                   >  2:1055989/1‑139 (MQ=255)
                                             tGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTTCCCTGAGAAGTGAGCGCAGGGAGGGGTTATCTTTCctga                                                                                              >  2:1457591/1‑140 (MQ=255)
                                                   tAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGGTTATCTTGTCTGACTGAA‑c                                                                                       >  2:225908/1‑140 (MQ=255)
                                                     aCCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTCGCATGCGGAGGGTGAGCTGCGGGGGGTTTTGTTGCCTTACTGGA‑cag                                                                                     >  2:1031299/1‑138 (MQ=255)
                                                          tataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAA‑CT‑GGCGAt                                                                               <  2:1355493/140‑1 (MQ=255)
                                                                 tCCATACCTATACTAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCC‑TTTTTTTTTTTTTTTCCCTTTGAAGTGGGCGCGGAGAGGGGTTATTTTGCCTTGCTTTA‑CT‑GGCGGCTTAGttt                                                                        >  2:1483785/1‑140 (MQ=255)
                                                                 tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCC‑TTTTTTTTTTTTTTTCCATTAGAAGGGAGGGCAGGGAGGGGTTATCTTTCCTGACTGAA‑CT‑GGCGGTTAAGttt                                                                        >  2:1459118/1‑140 (MQ=255)
                                                                 tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTGCCCTGAGAAGTGAGCGCAGAGAGGGGTTATCTTGCCTGACTTAA‑CT‑GGCGATTAAGttt                                                                        >  2:1593171/1‑140 (MQ=255)
                                                                 tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCA‑TTTTTTTTTTTTTTTCCCTGAGAAGTGAGCGCAGAGAGGGATTATTTTGCCTGACTTAA‑CT‑GGCGATTTAGttt                                                                        >  2:145592/1‑140 (MQ=255)
                                                                  ccATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTTCCCTG‑GAAAGGAGCGCCGAGAGGGGGTATTTTTCCTGACTGAACCT‑GGGGTTTAAGttt                                                                        >  2:804043/1‑140 (MQ=255)
                                                                  ccATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAA‑CT‑GGCGATTAAGTTTc                                                                       <  1:300174/140‑1 (MQ=255)
                                                                          ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCC‑TTTTTTTTTTTTTTGCCCTGAGAAGTGAGGGCAGAGAGGGGTTATCTTTCCTGACTGAA‑CT‑GGGGATTAAAGTTCCCCGTAcc                                                               >  2:197480/1‑140 (MQ=255)
                                                                          ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAA‑CT‑GGCGATTAAGTTTCCGCGTAcc                                                               <  1:1031299/140‑1 (MQ=255)
                                                                          ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAA‑CT‑GGCGATTAAGTTTCCGCGTAcc                                                               <  1:789725/140‑1 (MQ=255)
                                                                           taCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTTCCCTGAGAAGTGAGCGCAGAGAGGGGGTATCTTTCCTGACTGAA‑CT‑GGCGGTTAAGTTTTCGCGGAccc                                                              >  2:749801/1‑140 (MQ=255)
                                                                           taCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAA‑CT‑GGCGATTAAGTTTCCGCGTAccc                                                              <  2:340122/140‑1 (MQ=255)
                                                                           taCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAA‑CT‑GGCGATTAAGTTTCCGCGTAccc                                                              <  1:225908/140‑1 (MQ=255)
                                                                            aCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAA‑CT‑GGCGATTAAGTTTCCGCGTACCCg                                                             <  1:867033/140‑1 (MQ=255)
                                                                             ccAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTCTTCCATGAGAAGTGAGGGCAGAGAGGGGATATCTTTCCTCACTGAA‑CT‑GGCGGTTAAATTTTCGCGTAccccg                                                            >  2:295994/1‑138 (MQ=255)
                                                                                gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTTCCCTTAGAAGAGAGGGCAGAGAGGGGTTTTCTTTCCTGTCTGAA‑CT‑GGCGATTAAGATTTCGCGTTCCCGGCGt                                                         >  2:1365507/1‑140 (MQ=255)
                                                                                gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGGTTATCTTTCCTGACTGAA‑CT‑GGCGGTTAAGTTTTCGCGGACCCcgcgg                                                         >  2:1302222/1‑139 (MQ=255)
                                                                                gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTCTGCCATGAGAAGGGAGCGCAGAGAGGGATTATCTTGCCTGACTGAA‑CT‑GGCGATTAAGTTTCCGCGTACCCGGCGt                                                         >  1:507702/1‑140 (MQ=255)
                                                                                gTCAGGAGACATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTCTTCCCTGAGAAGGGAGAGCAGAGAGGGGTTATCTTTCCTGACTGTA‑CT‑GGCGTGTAAGTTTTCGCGTGCCCGGccg                                                         >  2:973306/1‑138 (MQ=255)
                                                                                 tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTTCCCTGGGAAGGGAGGGCAGAGAGGGGTTATCTTGCCTGACTGAA‑CT‑GGGGATTAAGTTTCCGCGGACCCGGCGtt                                                        >  2:423919/1‑140 (MQ=255)
                                                                                 tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTGCCCTGAGAAGTGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAA‑CT‑GGCGATTAAAGTTTCGCGGACCCCGCGtt                                                        >  2:659485/1‑140 (MQ=255)
                                                                                 tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTGCCCTGAGAAGGGAGCGCAGAGGGGGGTTATCTTGCCTTACTGAA‑CT‑GGCGGTTAAGGTTTCCCGTACCCCGCGtt                                                        >  2:474815/1‑140 (MQ=255)
                                                                                 tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTGCCCTGAGAAATGAGCGCAGAGAGGGGGTATCTTTCCTGACTGAA‑CT‑GGGGATTAAGTTTTCGCGTACCCCGCGtt                                                        >  2:789725/1‑140 (MQ=255)
                                                                                 tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTGCCATGAGAAGTGAGCGGAGAGAGGGGTTATCTTTCCTGACTGAA‑CT‑GGCGATTAAGTTTTCGCGTACCCcgcggt                                                        >  2:1444280/1‑138 (MQ=255)
                                                                                 tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGGGGGATTATCTTGCCTGACTGAA‑CT‑GGCGGTTAAGTTTTCTCGTGCCCGGCggt                                                        >  2:1357777/1‑138 (MQ=255)
                                                                                 tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTCTGCCCTGAGAAGTGAGCGCAGAGGGGGATTATCTTTCCTGACTGAA‑CT‑GGCGATTAAGTTTTCGCGTACCCGGCggt                                                        >  2:1099550/1‑138 (MQ=255)
                                                                                 tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAA‑CT‑GGCGATTAAGTTTCCGCGTACCCGGCGtt                                                        >  2:621231/1‑140 (MQ=255)
                                                                                  cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTTCCATGAGAAGGGAGCGCAGAGAGGGGTTATCTTTCCTTGCTGAA‑CT‑TGCGGTTAAATTTTCGCGTACCCGGCGgtt                                                       >  2:1121801/1‑140 (MQ=255)
                                                                                  cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTGCCCTGAGAAGTGAGGGCAGGGAGGGATTATCTTTCCTTACTGGA‑CT‑GGCGGTTTAGTTTTCGCGTTCCCCGCGttt                                                       >  2:337197/1‑140 (MQ=255)
                                                                                  cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTGTCTTGCCTGACTGAA‑CT‑GGCGATTAAGTTTCCGCGTACCCGGCGttt                                                       <  1:478708/140‑1 (MQ=255)
                                                                                  cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGACA‑TTTTTTTTTTTTTTGCCACGGGAAAAGGGGGCGAAGGGGGGTTTTCTTGTCTTATTTAA‑CTCGGCGGTAAGGTTTCC‑CGTGCCCgggggtt                                                       >  2:255522/1‑135 (MQ=255)
                                                                                   aGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTTTGCCATGAGAAGGGAGGGCAGAGAGGGATTATCTTGCCTGACTGAA‑CT‑GGCGATTAAGTTTCCGCGTACCCGGCGtttt                                                      >  1:192963/1‑139 (MQ=255)
                                                                                     gAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTCTGCCATTAGAAGTGAGCGCAGAGGGGGGTTATCTTTCCTGACTGAA‑CT‑GGCGGTTTAGTTTTCGCGTACCCCGCGtttttt                                                    >  2:566470/1‑137 (MQ=255)
                                                                                        tCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTTCCCTGAGAAGTGAGGGCAGAGAGGGGTTATTTTTCCTGACTGAA‑CT‑GGCGGTTAAGTTTCCCCGTACCCGGCGGTTTGTTAt                                                 >  2:1353950/1‑140 (MQ=255)
                                                                                          aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAA‑CT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTg                                               <  1:1357777/140‑1 (MQ=255)
                                                                                          aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAA‑CT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTg                                               <  2:1299842/140‑1 (MQ=255)
                                                                                           tttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTTCCCTGAGAAGTGAGGGCAGAGAGGGGGTATCTTTCCTGACTGAA‑CT‑GGCGGTTAAGTTTTCCCGTACCCCGCGGTTTGTtttttt                                              >  2:404766/1‑138 (MQ=255)
                                                                                           tttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTGCCATGAGAAGTGAGGGCAGAGAGGGGTTATCTTGCCTGACTGGA‑CT‑GGCGGTTAAGTTTCCGCGTACCCGGCGGTTTGTTATTgg                                              >  2:1255139/1‑139 (MQ=255)
                                                                                            ttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAA‑CT‑GGCGATTAAGTTTCCGCGTGCCCGGCGTTTAGTTATTGCg                                             <  1:973306/140‑1 (MQ=255)
                                                                                            ttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAA‑CT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCg                                             <  2:1445355/140‑1 (MQ=255)
                                                                                              cAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAA‑CT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGga                                           <  1:915569/140‑1 (MQ=255)
                                                                                              cAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAA‑CT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGga                                           <  1:966429/140‑1 (MQ=255)
                                                                                                  aTGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAA‑CT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgat                                       <  1:1159252/140‑1 (MQ=255)
                                                                                                   tgacATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGCATTATCTTGCCTGACTGAA‑CT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgatg                                      <  1:1005976/137‑1 (MQ=255)
                                                                                                    gCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCCTGAGAAGGGAGCGCAGAGAGGGATTTTCTTTGCTGGCTGAA‑CT‑GGCGATTAAGTTTTCGCGTACCCCGCGGTTTGTTATTGCGgatgatgg                                     >  2:95043/1‑139 (MQ=255)
                                                                                                      cATGTCGCGGATCTCGTTTGCGGCGGTCC‑TTTTTTTTTTTTTTTCCATGAGAAGTGAGCGCAGAGAGGGGTTATCTTTCCTGACTGAA‑CT‑GGCGGTTAAGTTTCCGCGTACCCGGCGTTTTGTTATTGCGGGTGATGata                                   >  2:318409/1‑140 (MQ=255)
                                                                                                               gATCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTTCCATGAGAAGGGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAA‑CT‑GGCGATTAAGTTTTCGCGGACCCCGCGGTTTGTTATTGCGGGTGATGATATTTAAACTc                          >  2:545038/1‑140 (MQ=255)
                                                                                                                aTCTCGTTTGCGGCGGTCC‑TTTTTTTTTTTTTTGCCCTTAGAAGTGAGCGCAGAGAGGGGTTATCTTTCCTGACTGGA‑CT‑GGCGGTTAAATTTCCCCGGACCCGGCGTTTTGTTATTTCGGGTGATGGTATTTAAGCTCg                         >  2:1085020/1‑140 (MQ=255)
                                                                                                                aTCTCGTTTGCGGCGGTCA‑TTTTTTTTTTTTTTGCCCTGAGAAGTGAGCGCAGAGGGGGGTTATCTTTCCTGACTGAA‑CT‑GGCGGTTAAGTTTTCGCGTACCCGGCGTTTTGGTATTGCGGGTGATGAAATTTAAGCTCg                         >  2:844866/1‑140 (MQ=255)
                                                                                                                     gTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAA‑CT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGa                    <  1:659485/140‑1 (MQ=255)
                                                                                                                      tttGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAA‑CT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGAt                   <  1:145592/140‑1 (MQ=255)
                                                                                                                             cggTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAA‑CT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCa            <  2:1363803/140‑1 (MQ=255)
                                                                                                                               gTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAA‑CT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCAtt          <  1:474815/140‑1 (MQ=255)
                                                                                                                                tCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAA‑CT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTg         <  1:804043/140‑1 (MQ=255)
                                                                                                                                 cA‑TTTTTTTTTTTTTTGCCATGAGAAGGGAGCGCAGAGGGGGGTTATCTTTCCCTACTGAA‑CT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTTGTTTTTTCGGATGGTGATATTTAAGCTCGGCTGGTTTGTTTAtttt        >  2:1115176/1‑138 (MQ=255)
                                                                                                                                      ttttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAA‑CT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTTGTTCATTGTCGcc    >  2:678535/1‑140 (MQ=255)
                                                                                                                                      ttttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAA‑CT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGcc    <  2:877372/140‑1 (MQ=255)
                                                                                                                                        ttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAA‑CT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAt  <  1:1109939/140‑1 (MQ=255)
                                                                                                                                        ttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAA‑CT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAt  <  1:95043/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                           
ATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCA‑TTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAA‑CT‑GGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAT  >  NC_000913/4604207‑4604481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑