Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,404,2180TG30.8% 67.5 / 11.5 39G245G (GGT→GGG) yhdHacrylyl‑CoA reductase
Reads supporting (aligned to +/- strand):  ref base T (3/24);  new base G (12/0);  total (15/24)
Fisher's exact test for biased strand distribution p-value = 1.16e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCATT  >  NC_000913/3404082‑3404352
                                                                                                                                        |                                                                                                                                      
aGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCCGGGGGCTGGCGGGTGGgttt                                                                                                                                     >  1:1230869/1‑140 (MQ=255)
 gTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTa                                                                                                                                    <  2:352425/140‑1 (MQ=255)
  tttGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTAc                                                                                                                                   <  1:647450/140‑1 (MQ=255)
   ttGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTAct                                                                                                                                  <  2:1172078/140‑1 (MQ=255)
      ccGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTg                                                                                                                               <  2:746273/140‑1 (MQ=255)
      ccGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTg                                                                                                                               <  2:188642/140‑1 (MQ=255)
           tCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAAc                                                                                                                          <  1:383090/140‑1 (MQ=255)
                 ccTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGt                                                                                                                    <  2:418805/140‑1 (MQ=255)
                    ctGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAGGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCAt                                                                                                                 <  2:1225711/140‑1 (MQ=255)
                    ctGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCAt                                                                                                                 <  2:753378/140‑1 (MQ=255)
                      ggAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGTTTTACTCTGCCAACCACGGTCATGc                                                                                                               >  1:1067487/1‑140 (MQ=255)
                      ggAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTTCCCACCCCCGGCATGc                                                                                                               >  2:651940/1‑140 (MQ=255)
                         aaaaCAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCAt                                                                                                            <  2:990656/140‑1 (MQ=255)
                            aCAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCCGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTACTCTGCCAACCACGGGCATGCCAtttt                                                                                                         >  2:991084/1‑139 (MQ=255)
                               aGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGTTTTATTCTGCCAACCACGGGCATGCCATTTAttt                                                                                                      >  1:444754/1‑139 (MQ=255)
                               aGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTGCCAACCACGGGCATGCCATTTAttt                                                                                                      >  2:497212/1‑139 (MQ=255)
                                        tgggGCAATTGGCACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATg                                                                                             <  1:834704/140‑1 (MQ=255)
                                        tgggGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATg                                                                                             <  2:1134321/140‑1 (MQ=255)
                                          gggCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTTCTCTGCCAACCACGGGCATGCCATTTTTTCTGCGTAATGTc                                                                                           >  1:1240158/1‑140 (MQ=255)
                                          gggCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTATCTCCCAACCCCGGGCCTGCCCTTTTATCTGCGGAATGTc                                                                                           >  1:906166/1‑140 (MQ=255)
                                           ggCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTGCCAACCACGGGCATGGCATTTTTTCTGCGGAATGTcc                                                                                          >  2:662739/1‑140 (MQ=255)
                                               aTTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTACTCTGCCCACCACGGTCATGCCCTTTTTTCTTCGTAATGTTCCttt                                                                                      >  2:8140/1‑140 (MQ=255)
                                                ttGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTGCCAACCACGGGCATGCCCTTTTTTTTGCGTAATGTCCGtttt                                                                                     >  2:181496/1‑139 (MQ=255)
                                                 tGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGc                                                                                    <  2:745619/140‑1 (MQ=255)
                                                  gACACCGTTGGTGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCa                                                                                   <  2:1067487/140‑1 (MQ=255)
                                                  gACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCa                                                                                   <  1:497212/140‑1 (MQ=255)
                                                  gACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCa                                                                                   <  1:181496/140‑1 (MQ=255)
                                                   acacCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTGCTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCaa                                                                                  <  1:723887/140‑1 (MQ=255)
                                                   acacCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCaa                                                                                  <  2:515502/140‑1 (MQ=255)
                                                   acacCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCaa                                                                                  <  2:175352/140‑1 (MQ=255)
                                                   acacCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCaa                                                                                  <  2:911585/140‑1 (MQ=255)
                                                    cacCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTGCCAACCACGGGCATGCCATTTATTCTGCGGAATGTCCCTTTGCAAg                                                                                 >  2:840671/1‑140 (MQ=255)
                                                        gTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGt                                                                             <  2:34026/140‑1 (MQ=255)
                                                        gTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGt                                                                             <  1:651940/140‑1 (MQ=255)
                                                         ttGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTg                                                                            >  1:155317/1‑140 (MQ=255)
                                                          tGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTACTCTGCCAACCCCGGTCATGCCATTTTTTTTGCGTAATGTCCGTTTGCAAGgggggg                                                                           >  2:707596/1‑140 (MQ=255)
                                                          tGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGGTTTCAAGGGGTgg                                                                           >  1:124803/1‑140 (MQ=255)
                                                            gCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGAt                                                                         <  1:991084/140‑1 (MQ=255)
                                                                        tGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGGCTGGCGGGGGGGTTTTCTCTGCCAACCACCGGCATGCCATTTTTTTTTCGGGAAGTCCGGTTTTCAGGGGGGGGTTTAGGAATGAcc                                                             >  2:36461/1‑139 (MQ=255)
                                                                                           aaTGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGGTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGGGGATTCAGTAATGACGCCACCAGAACGCCGCGcac                                          >  1:639400/1‑140 (MQ=255)
                                                                                                      gcggcTGCGTGGCGGCCTGTGGTCTGGCGGGTGGGTTTACTCTTCCAACCACCGTTAAGCCATTTTTTCTTCGGAATGTCCGTTTGCAAGGGGGGGATTTAGTAATCAAGCCCACAGAAAGCCGCGCCCAAAGCTGGCAg                               >  2:798689/1‑140 (MQ=255)
                                                                                                      gcggcTGCGTGGCGGCCTGTGGTCTGGCGGGTGGGTTTACTCTGCCAACCCCGGTCATGCCCTTTATTCTGCGGAATGTCCCTTTGCAAGGGGGGGATTCAGTAATGACGCCACCCGAAAGCCGCGCACAAGCCCGGCAg                               >  2:442989/1‑140 (MQ=255)
                                                                                                                       tgtgGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGAttt              >  2:1098635/1‑140 (MQ=255)
                                                                                                                       tgtgGTCTGGCGGGGGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGAttt              >  2:148778/1‑140 (MQ=255)
                                                                                                                              tGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGaa       <  1:36461/140‑1 (MQ=255)
                                                                                                                                   ggtggtTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCAtt  >  1:159018/1‑140 (MQ=255)
                                                                                                                                   ggtggtTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCAtt  <  1:840671/140‑1 (MQ=255)
                                                                                                                                   ggtggtTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAACCTGGCAGCGACTGGTCGCCGATTTACCGGAATCAtt  <  2:906166/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                      
AGTTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCATT  >  NC_000913/3404082‑3404352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑