Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913501,5640AC25.0% 71.1 / 13.9 36*559E (TAA→GAA) ybaLputative transporter YbaL
Reads supporting (aligned to +/- strand):  ref base A (23/4);  new base C (0/9);  total (23/13)
Fisher's exact test for biased strand distribution p-value = 7.59e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGC  >  NC_000913/501426‑501698
                                                                                                                                          |                                                                                                                                      
ttAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTAc                                                                                                                                       <  2:86896/140‑1 (MQ=255)
ttAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTAc                                                                                                                                       >  1:841726/1‑140 (MQ=255)
ttAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTAc                                                                                                                                       <  2:1312582/140‑1 (MQ=255)
 tAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTAcc                                                                                                                                      <  1:852018/140‑1 (MQ=255)
   aGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTAcccc                                                                                                                                    <  2:792822/140‑1 (MQ=255)
             ggTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCt                                                                                                                          >  1:299009/1‑140 (MQ=255)
               ttCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCa                                                                                                                        >  2:1623804/1‑140 (MQ=255)
                  gTAGGCCAGATAAGGCGTTCACGGCGCATCTGGCATTTGGCTCTCGATACCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCAccc                                                                                                                     >  1:892922/1‑140 (MQ=255)
                           aTAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGc                                                                                                            >  1:1005638/1‑140 (MQ=255)
                           aTAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGc                                                                                                            >  1:903606/1‑140 (MQ=255)
                                   ttCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCcag                                                                                                    >  2:354880/1‑140 (MQ=255)
                                   tccccgccgcATCTGGTTTTTGGCTCTCGTTGCCTGATGCGCCGCGGGCGCTTCTTATCATCCCTAATTTTTTTTCATTTTTTACACCCGGCACCCCCCTTTTCCCCCGCCCCCCCCTCCCCCGCCGGTGGCGTTTCcag                                                                                                    <  2:806613/136‑1 (MQ=255)
                                    tCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCcagc                                                                                                   >  2:18325/1‑140 (MQ=255)
                                    tCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCcagc                                                                                                   >  1:1639369/1‑140 (MQ=255)
                                     cACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCcagca                                                                                                  >  1:1423898/1‑140 (MQ=255)
                                          cgcATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTcc                                                                                             >  1:559489/1‑140 (MQ=255)
                                          cccttatGGCTTTTGCCTCTCGATGCCTGATGCGCCGCGGGCGCGTTTTATCATCCCTACATTTTTTTAATATTTTACACCCGGCAACCCCCGTTTCCCCCGTCCCCACCTCCCCCGCCGGTGGCGTTTCCAGCAGTTcc                                                                                             <  2:1354056/134‑1 (MQ=255)
                                                            ctcGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGAtt                                                                           >  2:1240125/1‑140 (MQ=255)
                                                               gATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCa                                                                        >  1:1007363/1‑140 (MQ=255)
                                                                 tGCCTGATGCGACGCTGGCGCGTCTTATCATCCCTACATTTTTTTCATATTTTACCCCCGGCAACCCCCGTTCCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACg                                                                      <  2:85135/140‑1 (MQ=38)
                                                                  gCCTGATGCGCCGCTGGCGCTTTTTATCATCCCTACTTTTTTTTCATTTTTTACACCCGCCACCCCCCTTTTCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGc                                                                     <  2:1201527/140‑1 (MQ=255)
                                                                  gCCTGATGCGACGCGGGGGCGTTTTCTGGTGCCTATTTTTTTTGCATTTTTTGCACCCGGCAACCCCCGTTTCCCCCGTCCCCCCCCCGCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGc                                                                     <  2:108151/140‑1 (MQ=255)
                                                                  gCCTGAGGCGCCGCTTGCGCTTTTTATCATCCCTAATTTTTTTTCATATTTTCCATCCGGCAACCCCCTTTTACCCCGTCACCCCCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGc                                                                     <  2:508904/140‑1 (MQ=255)
                                                                  gCCTGAGGCGACGCTGGCGCGTTTTATCACGCCTACATTTTTTTCATATTTTACACCCGGCACCCCCCGTTTCCCCCGCCCCCCCCCCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGc                                                                     <  2:1423898/140‑1 (MQ=255)
                                                                       aTGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTCCCCCGTCACCACCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGcc                                                                <  1:227816/140‑1 (MQ=255)
                                                                            aCGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTCCCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCaaca                                                           <  1:1609810/140‑4 (MQ=255)
                                                                             cGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCAc                                                          >  1:251138/1‑140 (MQ=255)
                                                                                gggCGCGTCTTATCATGCCTACATTTTTTTCATTTTTTACATCCGGCAACCCCCGTTTCCCCCGTCACCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTAc                                                       <  1:18325/139‑1 (MQ=255)
                                                                                         ttACCATGCCTAAATTTTTTTCATTTTTTACACCCGGCACCCCCCTTTTCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGc                                              <  2:892922/140‑1 (MQ=255)
                                                                                            tCATGCCTATATTTTTTTCATTTTTTACACCCGGCACCCCCCTTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGcacc                                           <  2:1248418/140‑1 (MQ=255)
                                                                                             taTGCCTACATTTTTTTCATATTTTACACCCGGCAACCGCCTTTTCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGcacca                                          <  2:864057/139‑1 (MQ=255)
                                                                                                 ccTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGtt                                      >  1:564215/1‑140 (MQ=255)
                                                                                                 ccTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGtt                                      >  2:1550345/1‑140 (MQ=255)
                                                                                                  cTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTc                                     >  2:846838/1‑140 (MQ=255)
                                                                                                   tACATTTTTTTCATATTTTACATCCGGCAACCACCGTTTCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCg                                    <  1:601291/140‑1 (MQ=255)
                                                                                                    aattatTTTTCATATTTTACCCCCGCCACCCCCCTTTCCCCCCCTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCgg                                   <  2:594456/137‑1 (MQ=255)
                                                                                                    aCATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCgg                                   >  1:397832/1‑140 (MQ=255)
                                                                                                     cATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGt                                  >  1:132044/1‑140 (MQ=255)
                                                                                                     cATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGt                                  >  1:1265213/1‑140 (MQ=255)
                                                                                                      atatTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTg                                 >  1:929644/1‑140 (MQ=255)
                                                                                                           tttCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGAtata                            >  2:39704/1‑140 (MQ=255)
                                                                                                             tCATATTTTACACCGGGCAACCCCGGTTTCCCCCGTCACCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACg                          <  1:1623804/140‑1 (MQ=255)
                                                                                                                     tACACCCGGCAACCCCCGTTTCCCCCGTCCCCACCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCa                  <  2:1022097/140‑1 (MQ=255)
                                                                                                                       cATCCGGCACCCCCCTTTTCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATc                <  2:900790/140‑1 (MQ=255)
                                                                                                                         cccGGCACCCCCCTTTTCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGt              <  2:986493/139‑1 (MQ=255)
                                                                                                                             gCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATa          >  2:624811/1‑140 (MQ=255)
                                                                                                                              cacccaccGTTTCCCCCGTCACCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATaa         <  1:90855/137‑1 (MQ=255)
                                                                                                                                     cGTTTCCCCCCTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGgcgc  <  2:1265213/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                      
TTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGC  >  NC_000913/501426‑501698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑