Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,821,727	G→T	G95G (GGC→GGA)	alaS ←	alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,821,727	0	G	T	100.0%	328.0 / NA	93	G95G (GGC→GGA)	alaS	alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (46/47);  total (46/47)

TAGGCTTCGTCGTCGCTTTCATAGACGGTAACCCACAGACGCTCTTTCGGCAGGGCAAACCATTTTTCGCTGGTCAGCAGTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTGCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACA  >  NC_000913/2821593‑2821863                                                                                                                                       |                                                                                                                                         tAGGCTTCGTCGTCGCTTTCATAGACGGTAACCCACAGACGCTCTTTCGGCAGGGCAAACCATTTTTCGCTGGTCAGCAGTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAg                                                                                                                                     >  2:1489413/1‑140 (MQ=255)     cTTCGTCGTCGCTTTCATAGACGGTAACCCACAGACGCTCTTTCGGCAGGGCAAACCATTTTTCGCTGGTCAGCAGTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCAtt                                                                                                                                 >  2:1431442/1‑140 (MQ=255)     cTTCGTCGTCGCTTTCATAGACGGTAACCCACAGACGCTCTTTCGGCAGGGCAAACCATTTTTCGCTGGTCAGCAGTCCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCAtt                                                                                                                                 <  1:1247993/140‑1 (MQ=255)         gtcgtcgCTTTCATAGACGGTAACCCACAGACGCTCTTTCGGCAGGGCAAACCATTTTTCGCTGGTCAGCAGGTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCga                                                                                                                             >  1:1039698/1‑140 (MQ=255)          tcgtcgCTTTCATAGACGGTAACCCACAGACGCTCTTTCGGCAGGGCAAACCATTTTTCGCTGGTCAGCAGTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCgaa                                                                                                                            <  2:1039698/140‑1 (MQ=255)          tcgtcgCTTTCATAGACGGTAACCCACAGACGCTCTTTCGGCAGGGCAAACCATTTTTCGCTGGTCAGCAGTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCgaa                                                                                                                            <  2:242737/140‑1 (MQ=255)          tcgtcgCTTTCATAGACGGTAACCCACAGACGCTCTTTCGGCAGGGCAAACCATTTTTCGCTGGTCAGCAGTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCgaa                                                                                                                            <  1:58196/140‑1 (MQ=255)          tcgtcgCTTTCATAGACGGTAACCCACAGACGCTCTTTCGGCAGGGCAAACCATTTTTCGCTGGTCAGCAGTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCgaa                                                                                                                            <  1:1511624/140‑1 (MQ=255)           cgtcgCTTTCATAGACGGTAACCCACAGACGCTCTTTCGGCAGGGCAAACCATTTTTCGCTGGTCAGCAGTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCgaag                                                                                                                           <  1:979435/140‑1 (MQ=255)           cgtcgCTTTCATAGACGGTAACCCACAGACGCTCTTTCGGCAGGGCAAACCATTTTTCGCTGGTCAGCAGTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCgaag                                                                                                                           >  2:979435/1‑140 (MQ=255)           cgtcgCTTTCATAGACGGTAACCCACAGACGCTCTTTCGGCAGGGCAAACCATTTTTCGCTGGTCAGCAGTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCgaag                                                                                                                           <  2:390157/140‑1 (MQ=255)            gtcgCTTTCATAGACGGTAACCCACAGACGCTCTTTCGGCAGGGCAAACCATTTTTCGCTGGTCAGCAGTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCgaaga                                                                                                                          <  1:4049/140‑1 (MQ=255)            gtcgCTTTCATAGACGGTAACCCACAGACGCTCTTTCGGCAGGGCAAACCATTTTTCGCTGGTCAGCAGTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCgaaga                                                                                                                          <  1:994513/140‑1 (MQ=255)            gtcgCTTTCATAGACGGTAACCCACAGACGCTCTTTCGGCAGGGCAAACCATTTTTCGCTGGTCAGCAGTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCgaaga                                                                                                                          <  2:245324/140‑1 (MQ=255)            gtcgCTTTCATAGACGGTAACCCACAGACGCTCTTTCGGCAGGGCAAACCATTTTTCGCTGGTCAGCAGTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCgaaga                                                                                                                          <  2:909430/140‑1 (MQ=255)            gtcgCTTTCATAGACGGTAACCCACAGACGCTCTTTCGGCAGGGCAAACCATTTTTCGCTGGTCAGCAGTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCgaaga                                                                                                                          <  1:1439250/140‑1 (MQ=255)              cgCTTTCATAGACGGTAACCCACAGACGCTCTTTCGGCAGGGCAAACCATTTTTCGCTGGTCAGCAGTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGaagaag                                                                                                                        >  1:270630/1‑140 (MQ=255)                       aGACGGTAACCCACAGACGCTCTTTCGGCAGGGCAAACCATTTTTCGCTGGTCAGCAGTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGa                                                                                                               >  1:36648/1‑140 (MQ=255)                           ggTAACCCACAGACGCTCTTTCGGCAGGGCAAACCATTTTTCGCTGGTCAGCAGTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGAcgcg                                                                                                           <  2:1338537/140‑1 (MQ=255)                           ggTAACCCACAGACGCTCTTTCGGCAGGGCAAACCATTTTTCGCTGGTCAGCAGTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGAcgcg                                                                                                           >  1:322579/1‑140 (MQ=255)                             tAACCCACAGACGCTCTTTCGGCAGGGCAAACCATTTTTCGCTGGTCAGCAGTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGAcgcgcg                                                                                                         >  1:1198419/1‑140 (MQ=255)                             tAACCCACAGACGCTCTTTCGGCAGGGCAAACCATTTTTCGCTGGTCAGCAGTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGAcgcgcg                                                                                                         >  2:262061/1‑140 (MQ=255)                             tAACCCACAGACGCTCTTTCGGCAGGGCAAACCATTTTTCGCTGGTCAGCAGTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGAcgcgcg                                                                                                         >  1:682703/1‑140 (MQ=255)                             tAACCCACAGACGCTCTTTCGGCAGGGCAAACCATTTTTCGCTGGTCAGCAGTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGAcgcgcg                                                                                                         >  2:983423/1‑140 (MQ=255)                             tAACCCACAGACGCTCTTTCGGCAGGGCAAACCATTTTTCACCGGTCAGCTGTTCCCATGCAAACTGAATGGCATCGTGTTTGCAATAATAGCCGACGCTGAAGTTTCCCAGCATTTCGAAGCAGGAATGGAAAcgcgcg                                                                                                         >  2:1022375/1‑140 (MQ=255)                               aCCCACAGACGCTCTTTCGGCAGGGCAAACCATTTTTCGCTGGTCAGCAGTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGAcg                                                                                                             <  1:40724/134‑1 (MQ=255)                               aCCCACAGACGCTCTTTCGGCAGGGCAAACCATTTTTCGCTGGTCAGCAGTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGAcg                                                                                                             >  2:40724/1‑134 (MQ=255)                               aCCCACAGACGCTCTTTCGGCAGGGCAAACCATTTTTCGCTGGTCAGCAGTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGgt                                                                                                       >  2:896103/1‑140 (MQ=255)                                     aGACGCTCTTTCGGCAGGGCAAACCATTTTTCGCTGGTCAGCAGTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAAcc                                                                                                 >  2:1511565/1‑140 (MQ=255)                                     aGACGCTCTTTCGGCAGGGCAAACCATTTTTCGCTGGTCAGCAGTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAAcc                                                                                                 >  2:303772/1‑140 (MQ=255)                                      gACGCTCTTTCGGCAGGGCAAACCATTTTTCGCTGGTCAGCAGTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCg                                                                                                >  2:770455/1‑140 (MQ=255)                                        cGCTCTTTCGGCAGGGCAAACCATTTTTCGCTGGTCAGCAGTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGAc                                                                                              <  1:476130/140‑1 (MQ=255)                                        cGCTCTTTCGGCAGGGCAAACCATTTTTCGCTGGTCAGCAGTTCCCATCCAGACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGAc                                                                                              <  1:1060260/140‑1 (MQ=255)                                         gCTCTTTCGGCAGGGCAAACCATTTTTCGCTGGTCAGCAGTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACg                                                                                             <  1:1511565/140‑1 (MQ=255)                                         gCTCTTTCGGCAGGGCAAACCATTTTTCGCTGGTCAGCAGTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACg                                                                                             <  2:935629/140‑1 (MQ=255)                                            ctTTCGGCAGGGCAAACCATTTTTCGCTGGTCAGCAGTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGttt                                                                                          >  2:1123282/1‑140 (MQ=255)                                             tttCGGCAGGGCAAACCATTTTTCGCTGGTCAGCAGTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGAATGGTGACGCGCGGCGTAACCGACGtttt                                                                                         >  1:1484175/1‑140 (MQ=255)                                              ttCGGCAGGGCAAACCATTTTTCGCTGGTCAGCAGTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTc                                                                                        >  1:1136277/1‑140 (MQ=255)                                              ttCGGCAGGGCAAACCATTTTTCGCTGGTCAGCAGTTCCCATGCAAACTGAATGACATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTc                                                                                        >  2:777407/1‑140 (MQ=255)                                                 ggCAGGGCAAACCATTTTTCGCTGGTCAGCAGTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAg                                                                                     >  1:1220517/1‑140 (MQ=255)                                                     gggCAAACCATTTTTCGCTGGTCAGCAGTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCg                                                                                 >  2:1543307/1‑140 (MQ=255)                                                     gggCAAACCATTTTTCGCTGGTCAGCAGTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCg                                                                                 <  2:1333995/140‑1 (MQ=255)                                                        cAAACCATTTTTCGCTGGTCAGCAGTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTtg                                                                              <  2:1198419/140‑1 (MQ=255)                                                                ttttCGCTGGTCAGCAGTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTacc                                                                      >  2:1366463/1‑140 (MQ=255)                                                                 tttCGCTGGTCAGCAGTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTacca                                                                     >  1:498996/1‑140 (MQ=255)                                                                   tCGCTGGTCAGCAGTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTaccacc                                                                   <  2:1521268/140‑1 (MQ=255)                                                                   tCGCTGGTCAGCAGTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGACGTTGTGTTTaccacc                                                                   <  2:192231/140‑1 (MQ=255)                                                                       tGGTCAGCAGTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCcgca                                                               >  2:1371102/1‑140 (MQ=255)                                                                        ggTCAGCAGTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCcgcac                                                              <  2:370460/140‑1 (MQ=255)                                                                         gTCAGCAGTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCcgcacg                                                             >  2:993384/1‑140 (MQ=255)                                                                             gcagTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCcgcacgcacg                                                         <  1:896103/140‑1 (MQ=255)                                                                             gcagTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCcgcacgcacg                                                         <  1:221095/140‑1 (MQ=255)                                                                               agTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCcgcacgcacgca                                                       <  2:322579/140‑1 (MQ=255)                                                                                   cccATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAGGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGt                                                   >  1:1042802/1‑140 (MQ=255)                                                                                   cccATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGt                                                   <  1:1431442/140‑1 (MQ=255)                                                                                   cccATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGt                                                   >  2:400002/1‑140 (MQ=255)                                                                                     cATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTg                                                 <  2:727211/140‑1 (MQ=255)                                                                                      aTGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTgg                                                >  1:12054/1‑140 (MQ=255)                                                                                             cTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTgg                                         <  1:983423/140‑1 (MQ=255)                                                                                              tGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGt                                        >  1:305619/1‑140 (MQ=255)                                                                                              tGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGt                                        <  1:393420/140‑1 (MQ=255)                                                                                                aaTGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAg                                      >  1:1443516/1‑140 (MQ=255)                                                                                                  tGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAgcg                                    <  2:1136277/140‑1 (MQ=255)                                                                                                  tGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAgcg                                    >  2:479034/1‑140 (MQ=255)                                                                                                  tGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAgcg                                    <  1:303772/140‑1 (MQ=255)                                                                                                  tGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAgcg                                    >  2:399370/1‑140 (MQ=255)                                                                                                   ggCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAgcgc                                   <  1:1489413/140‑1 (MQ=255)                                                                                                     cATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCgg                                 >  2:763140/1‑140 (MQ=255)                                                                                                       tCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGa                               <  2:1220517/140‑1 (MQ=255)                                                                                                       tCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGa                               <  1:744488/140‑1 (MQ=255)                                                                                                        cGTGTTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaa                              >  2:324615/1‑140 (MQ=255)                                                                                                          tgtTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAGGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaata                            <  2:1042802/140‑1 (MQ=255)                                                                                                          tgtTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaata                            >  2:1143655/1‑140 (MQ=255)                                                                                                          tgtTTGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaata                            >  2:651554/1‑140 (MQ=255)                                                                                                            tttGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaataat                          <  1:770455/140‑1 (MQ=255)                                                                                                              tGAAATAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTa                        >  2:60696/1‑140 (MQ=255)                                                                                                                aaaTAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACg                      <  1:324615/140‑1 (MQ=255)                                                                                                                aaaTAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACg                      <  2:850766/140‑1 (MQ=255)                                                                                                                 aaTAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGc                     <  2:498996/140‑1 (MQ=255)                                                                                                                  aTAGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCt                    >  1:702760/1‑140 (MQ=255)                                                                                                                    aGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACGGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTg                  >  1:1286029/1‑140 (MQ=255)                                                                                                                    aGTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTg                  >  2:1504881/1‑140 (MQ=255)                                                                                                                     gTCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGt                 >  2:6033/1‑140 (MQ=255)                                                                                                                      tCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACg                      >  2:913478/1‑134 (MQ=255)                                                                                                                      tCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACg                      <  1:913478/134‑1 (MQ=255)                                                                                                                      tCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTc                >  2:565244/1‑140 (MQ=255)                                                                                                                      tCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTc                <  1:1366463/140‑1 (MQ=255)                                                                                                                      tCGCCGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACAACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTc                >  2:1308209/1‑140 (MQ=255)                                                                                                                          cGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGc            <  2:1484175/140‑1 (MQ=255)                                                                                                                          cGAAGCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGc            <  1:1123282/140‑1 (MQ=255)                                                                                                                              gCTGAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCaa        >  1:1119712/1‑140 (MQ=255)                                                                                                                                 gAAGTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGa     <  1:777407/140‑1 (MQ=255)                                                                                                                                    gTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAaca  <  2:12054/140‑1 (MQ=255)                                                                                                                                    gTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAaca  <  1:60696/140‑1 (MQ=255)                                                                                                                                    gTTTCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAaca  <  2:305619/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         TAGGCTTCGTCGTCGCTTTCATAGACGGTAACCCACAGACGCTCTTTCGGCAGGGCAAACCATTTTTCGCTGGTCAGCAGTTCCCATGCAAACTGAATGGCATCGTGTTTGAAATAGTCGCCGAAGCTGAAGTTGCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAACA  >  NC_000913/2821593‑2821863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑