BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,687,983	G→A	A132V (GCG→GTG)	fumA ←	fumarase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,687,983	0	G	A	98.9%	318.0 / ‑4.9	92	A132V (GCG→GTG)	fumA	fumarase A
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (52/39); minor base T (1/0); total (53/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 105 total aligned reads displayed.

ATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGC  >  NC_000913/1687846‑1688119

aTTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCAcc                                                                                                                                        >  1:594715/1‑140 (MQ=255)

    gtcgCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAgcg                                                                                                                                    >  2:1176467/1‑140 (MQ=255)

     tcgCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAgcgc                                                                                                                                   <  2:632166/140‑1 (MQ=255)

     tcgCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAgcgc                                                                                                                                   >  2:681863/1‑140 (MQ=255)

         cATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCt                                                                                                                               >  2:2227843/1‑140 (MQ=255)

         cATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCt                                                                                                                               >  1:2292657/1‑140 (MQ=255)

                ggCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATcac                                                                                                                        <  1:2279030/140‑1 (MQ=255)

                ggCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATcac                                                                                                                        <  2:1630218/140‑1 (MQ=255)

                ggCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATcac                                                                                                                        <  1:2374641/140‑1 (MQ=255)

                 gCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATcacc                                                                                                                       <  2:297199/140‑1 (MQ=255)

                 gCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATcacc                                                                                                                       <  1:532619/140‑1 (MQ=255)

                     aaaGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATcaccacca                                                                                                                   <  2:594715/140‑1 (MQ=255)

                     aaaGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACA‑CGCGCACCAGCGCCGCTTCATcaccaccac                                                                                                                  <  2:1327173/140‑1 (MQ=255)

                      aaGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATcaccaccac                                                                                                                  <  1:1783201/140‑1 (MQ=255)

                      aaGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATcaccaccac                                                                                                                  <  1:1893741/140‑1 (MQ=255)

                      aaGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATcaccaccac                                                                                                                  <  2:2168743/140‑1 (MQ=255)

                      aaGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATcaccaccac                                                                                                                  <  1:1176467/140‑1 (MQ=255)

                       aGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCaccaccacc                                                                                                                 <  2:2192417/140‑1 (MQ=255)

                       aGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCaccaccacc                                                                                                                 <  1:1569280/140‑1 (MQ=255)

                           cgatcTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTc                                                                                                             >  2:2384632/1‑140 (MQ=255)

                                   gcTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACg                                                                                                     <  2:769266/140‑1 (MQ=255)

                                      ggCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTg                                                                                                  <  1:2172514/140‑1 (MQ=255)

                                       gCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGc                                                                                                 >  1:1206434/1‑140 (MQ=255)

                                          gATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCt                                                                                              <  1:681863/140‑1 (MQ=255)

                                            ttGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCttt                                                                                            >  1:2375334/1‑140 (MQ=255)

                                              ggTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCttttt                                                                                          <  2:1489958/140‑1 (MQ=255)

                                                tGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTa                                                                                        <  1:2227843/140‑1 (MQ=255)

                                                tGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTa                                                                                        <  2:2304384/140‑1 (MQ=255)

                                                 gCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTAc                                                                                       >  1:1430042/1‑140 (MQ=255)

                                                  ccGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTAcc                                                                                      >  1:686028/1‑140 (MQ=255)

                                                     gTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACcaac                                                                                   >  2:471687/1‑140 (MQ=255)

                                                         tCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACaata                                                                               >  2:844067/1‑140 (MQ=255)

                                                          cACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACaataa                                                                              >  1:252346/1‑140 (MQ=255)

                                                                tttATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCgg                                                                        <  2:2292657/140‑1 (MQ=255)

                                                                    taCATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGcc                                                                    <  2:1430042/140‑1 (MQ=255)

                                                                    taCATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGcc                                                                    <  2:252346/140‑1 (MQ=255)

                                                                      cATATCCAGCGGCGCGTCTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCgg                                                                  >  1:129898/1‑140 (MQ=255)

                                                                       atatCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGt                                                                 >  2:773104/1‑140 (MQ=255)

                                                                         atCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCTCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTAt                                                               >  1:710612/1‑140 (MQ=255)

                                                                         atCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCATCACCGGTCCATACACGCTGCCCTTCTTTACCAACAATAATCGCgg                                                                        <  1:1494885/131‑1 (MQ=255)

                                                                         atCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGt                                                                 <  1:2311892/138‑1 (MQ=255)

                                                                         atCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTAt                                                               >  1:748704/1‑140 (MQ=255)

                                                                         atCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTAt                                                               >  2:2114849/1‑140 (MQ=255)

                                                                         atCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTAt                                                               >  2:1510717/1‑140 (MQ=255)

                                                                         atCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTAt                                                               >  1:1276976/1‑140 (MQ=255)

                                                                         atCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTAt                                                               >  1:2384518/1‑140 (MQ=255)

                                                                         atCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTAt                                                               >  1:2319822/1‑140 (MQ=255)

                                                                         atCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTAt                                                               >  1:2304276/1‑140 (MQ=255)

                                                                         atCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTAt                                                               >  1:2007584/1‑140 (MQ=255)

                                                                         atCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTCTTTACCAACAATAATCGCgg                                                                        >  2:1494885/1‑131 (MQ=255)

                                                                          tCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATc                                                              >  1:684659/1‑140 (MQ=255)

                                                                          tCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATc                                                              >  2:473697/1‑140 (MQ=255)

                                                                           ccAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATcc                                                             >  2:282884/1‑140 (MQ=255)

                                                                            cAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCt                                                            >  1:2208429/1‑140 (MQ=255)

                                                                              gcggcgCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGa                                                          >  1:436982/1‑140 (MQ=255)

                                                                              gcggcgCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGa                                                          >  1:296048/1‑140 (MQ=255)

                                                                              gcggcgCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGa                                                          <  2:2375334/140‑1 (MQ=255)

                                                                               cggcgCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTa                                                                >  2:1687720/1‑133 (MQ=255)

                                                                               cggcgCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTa                                                                <  1:1687720/133‑1 (MQ=255)

                                                                               cggcgCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGAc                                                         >  1:1472992/1‑140 (MQ=255)

                                                                               cggcgCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGAc                                                         >  1:357997/1‑140 (MQ=255)

                                                                                 gcgcGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACGGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAg                                                       >  2:876753/1‑140 (MQ=255)

                                                                                  cgcgTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAgg                                                      >  1:1278999/1‑140 (MQ=255)

                                                                                  cgcgTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAgg                                                      >  1:1611598/1‑140 (MQ=255)

                                                                                   gcgTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGt                                                     <  2:2169135/140‑1 (MQ=255)

                                                                                    cgTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGtt                                                    >  1:1596170/1‑140 (MQ=255)

                                                                                                       aTTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTcgccg                                 >  2:191370/1‑140 (MQ=255)

                                                                                                       aTTATCTTCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTcgccg                                 <  2:1276976/140‑1 (MQ=255)

                                                                                                             ttCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCGCCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGt                           <  2:296048/140‑1 (MQ=255)

                                                                                                             ttCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGt                           <  1:2203324/140‑1 (MQ=255)

                                                                                                             ttCGATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGt                           <  2:2208429/140‑1 (MQ=255)

                                                                                                                gATATAAGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCgg                        <  1:844067/140‑1 (MQ=255)

                                                                                                                  atataGTGGTATAGACACCGCGCAACAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGAAAGGATGGCAGAACGCCTTTCGCCGCGATGTCGGAg                      >  2:1536864/5‑140 (MQ=25)

                                                                                                                     aaGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTa                   >  2:854159/1‑140 (MQ=255)

                                                                                                                      aGTGTTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTAc                  <  1:854159/140‑1 (MQ=255)

                                                                                                                        tgtTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGc                <  2:2007584/140‑1 (MQ=255)

                                                                                                                        tgtTATAGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGc                <  1:76596/140‑1 (MQ=255)

                                                                                                                            ataGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGa            >  2:1713572/1‑140 (MQ=255)

                                                                                                                            ataGACACCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGa            >  1:1952895/1‑140 (MQ=255)

                                                                                                                                acacCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTg        >  1:2050624/1‑140 (MQ=255)

                                                                                                                                acacCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTg        <  1:191370/140‑1 (MQ=255)

                                                                                                                                  acCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCa      >  1:1314464/1‑140 (MQ=255)

                                                                                                                                  acCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCa      >  2:228073/1‑140 (MQ=255)

                                                                                                                                  acCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCa      >  2:1087749/1‑140 (MQ=255)

                                                                                                                                  acCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCa      >  2:1137925/1‑140 (MQ=255)

                                                                                                                                  acCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCa      >  2:371884/1‑140 (MQ=255)

                                                                                                                                  acCGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCa      >  2:1502934/1‑140 (MQ=255)

                                                                                                                                   ccGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAg     >  1:304642/1‑140 (MQ=255)

                                                                                                                                   ccGCGCACCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAg     <  2:874703/140‑1 (MQ=255)

                                                                                                                                    cgcgcaCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAgc    >  1:1270156/1‑140 (MQ=255)

                                                                                                                                    cgcgcaCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAgc    >  1:1014455/1‑140 (MQ=255)

                                                                                                                                    cgcgcaCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAgc    >  2:463231/1‑140 (MQ=255)

                                                                                                                                    cgcgcaCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAgc    >  2:279667/1‑140 (MQ=255)

                                                                                                                                    cgcgcaCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAgc    >  1:2245970/1‑140 (MQ=255)

                                                                                                                                    cgcgcaCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAgc    >  1:729832/1‑140 (MQ=255)

                                                                                                                                    cgcgcaCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAgc    >  2:1934173/1‑140 (MQ=255)

                                                                                                                                    cgcgcaCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCC‑TTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCacgc   >  2:2228714/1‑137 (MQ=255)

                                                                                                                                     gcgcacCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAgcg   <  2:1278999/140‑1 (MQ=255)

                                                                                                                                      cgcacCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAgcgc  >  1:1522922/1‑140 (MQ=255)

                                                                                                                                      cgcacCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAgcgc  <  2:1611598/140‑1 (MQ=255)

ATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGC  >  NC_000913/1687846‑1688119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`