Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,001,918	0	A	C	23.0%	201.3 / 23.9	87	intergenic (‑129/+192)	fliA/fliC	RNA polymerase, sigma 28 (sigma F) factor/flagellar filament structural protein
Reads supporting (aligned to +/- strand):  ref base A (63/4);  new base C (0/20);  total (63/24)
Fisher's exact test for biased strand distribution p-value = 4.47e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCA  >  NC_000913/2001779‑2002055                                                                                                                                             |                                                                                                                                           agTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑aaaaaaa                                                                                                                                            >  1:1177727/1‑140 (MQ=255)   tGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑cc                                                                                                                                         >  2:396005/1‑140 (MQ=255)   tGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑cc                                                                                                                                         >  2:1671322/1‑140 (MQ=255)     aaTTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑cccc                                                                                                                                       >  1:637240/1‑140 (MQ=255)      aTTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCccg                                                                                                                                      >  1:1179727/1‑140 (MQ=255)      aTTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCccg                                                                                                                                      >  2:2047719/1‑140 (MQ=255)      aTTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCccg                                                                                                                                      >  2:1686770/1‑140 (MQ=255)                          cGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGt                                                                                                                  >  1:177045/1‑140 (MQ=255)                               tatGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTg                                                                                                             >  1:1357938/1‑140 (MQ=255)                               tatGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTg                                                                                                             >  1:1422183/1‑140 (MQ=255)                                    gTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACaaa                                                                                                        >  2:673910/1‑140 (MQ=255)                                    gTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACaaa                                                                                                        >  1:973939/1‑140 (MQ=255)                                    gTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACaaa                                                                                                        >  2:1256961/1‑140 (MQ=255)                                    gTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACaaa                                                                                                        >  2:1267756/1‑140 (MQ=255)                                    gTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACaaa                                                                                                        >  1:1799743/1‑140 (MQ=255)                                    gTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACaaa                                                                                                        >  2:1782727/1‑140 (MQ=255)                                    gTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACaaa                                                                                                        >  2:1835701/1‑140 (MQ=255)                                    gTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACaaa                                                                                                        >  1:533065/1‑140 (MQ=255)                                    gTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACaaa                                                                                                        >  2:2151367/1‑140 (MQ=255)                                    gTTATCGGCATGATTATCCGTTTCTGCAGGGCTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACaaa                                                                                                        >  1:634735/1‑140 (MQ=255)                                      tATCGGCATGATTATCCGTTTCTGCAGGTTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAA‑AAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAAtt                                                                                                      <  2:2144139/140‑1 (MQ=255)                                      tATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGTGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCGGACAAAtt                                                                                                      >  2:551616/1‑140 (MQ=255)                                      tATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAAtt                                                                                                      >  1:678396/1‑140 (MQ=255)                                         cGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTgcg                                                                                                   >  1:456467/1‑140 (MQ=255)                                         cGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTgcg                                                                                                   >  2:1625570/1‑140 (MQ=255)                                         cGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTgcg                                                                                                   >  2:325700/1‑140 (MQ=255)                                               gATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTAt                                                                                             >  1:609060/1‑140 (MQ=255)                                                         ttCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGAt                                                                                   >  1:684685/1‑140 (MQ=255)                                                         ttCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGAt                                                                                   >  2:1853037/1‑140 (MQ=255)                                                         ttCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGAt                                                                                   >  1:1084777/1‑140 (MQ=255)                                                         ttCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCACCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGAt                                                                                   >  2:972132/1‑140 (MQ=255)                                                          tCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATg                                                                                  >  1:988054/1‑140 (MQ=255)                                                          tCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATg                                                                                  >  1:1019978/1‑140 (MQ=255)                                                          tCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATg                                                                                  >  2:1690628/1‑140 (MQ=255)                                                          tCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATg                                                                                  >  1:2085718/1‑140 (MQ=255)                                                           cTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgc                                                                                 >  1:1587798/1‑140 (MQ=255)                                                             gCAGGTTTTTTAATCGGACGATTAGTGGGTAAAATGAGGGGTTATTGGGGGGTTACAGGTAAATTCCAGGCAA‑AAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgcg                                                                                <  2:1871597/140‑1 (MQ=255)                                                              cAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgcggc                                                                              >  2:64224/1‑140 (MQ=255)                                                                 ggTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAGAAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGt                                                                            >  1:1871597/1‑140 (MQ=255)                                                                  gTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTaa                                                                          >  2:1650386/1‑140 (MQ=255)                                                                  gTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTaa                                                                          >  1:1482774/1‑140 (MQ=255)                                                                     tttAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACg                                                                       >  1:743277/1‑140 (MQ=255)                                                                      ttAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGc                                                                      >  1:1861568/1‑140 (MQ=255)                                                                           cGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTAt                                                                 >  2:322155/1‑140 (MQ=255)                                                                            ggACGATTAGTGGGTAAAATGGGGGTTTATTGGGGGGTTACAGGTAAATCCCAGGCAA‑AAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATc                                                                <  2:1601302/140‑1 (MQ=39)                                                                            ggACGATTAGGGGGTGAAATGAGGGGTTATTGGGGGGTTACAGGTAAATCCCAGGAAA‑AAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATc                                                                <  2:1179727/140‑1 (MQ=39)                                                                             gACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATcc                                                               >  2:28310/1‑140 (MQ=255)                                                                             gACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATcc                                                               >  2:209783/1‑140 (MQ=255)                                                                             gACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATcc                                                               >  1:2064511/1‑140 (MQ=255)                                                                             gACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTAATcc                                                               >  2:59693/1‑140 (MQ=255)                                                                              aagATTGGTGGGTAAAGGGGGGGGTTGTTTGGGGGTTACAGAAAAATCCCGGGAAG‑AAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCg                                                              <  2:1019978/138‑1 (MQ=37)                                                                              aCGATTAGTGGGTAAAATGAGGGTTTTTTGGGGGTTTACAGGTAAATCCCAGAAAA‑AAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCg                                                              <  2:988054/140‑1 (MQ=37)                                                                                gATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                            <  1:1889829/140‑1 (MQ=255)                                                                                atttAGTGGGTGAAATGGGGGTTTATTGGGGGTTTACAGAAAAATCCCAGGAAA‑AAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                            <  2:533065/138‑1 (MQ=37)                                                                                  ttAGTGGGTGAAAGGGGGGGTTTTTGGGGGGTTACAGGTAAATTCCAGGAAA‑AAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCt                                                          <  2:1084777/140‑1 (MQ=38)                                                                                      ggggTAAAATGGGGGTTTTTTGGGGGGTTACGGGTAAATTCCAGGAAA‑AAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaa                                                      <  2:385690/139‑1 (MQ=37)                                                                                            aaaTGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAtgt                                                >  2:1066162/1‑140 (MQ=255)                                                                                            aaaTGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAtgt                                                >  1:1064188/1‑140 (MQ=255)                                                                                              aggAGGGTTTTTTGGGGGGTTACAGGTAAATTCCAGGAAA‑AAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGc                                              <  2:2085718/138‑1 (MQ=37)                                                                                                   gggTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAAtt                                         >  2:282633/1‑140 (MQ=255)                                                                                                      ttATTGGGGGGTTACAGGTAAATTCCTGGCAA‑AAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCaa                                      <  1:96187/140‑1 (MQ=255)                                                                                                        aTTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATa                                    >  1:461408/1‑140 (MQ=255)                                                                                                        aTTGGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACACCTTATCCGGCCTACAAAAATGTGCAAATTCAATa                                    <  1:1853037/140‑1 (MQ=255)                                                                                                         tttGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATaa                                   >  1:998185/1‑140 (MQ=255)                                                                                                           ggggggTTAAAGGTAAATCCCAGGAAA‑AAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAAt                                 <  2:1861568/139‑1 (MQ=37)                                                                                                            gggggTTACAGGCAAATCCGGGGAAA‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAAtt                                <  2:2064511/140‑1 (MQ=37)                                                                                                             ggggTTACATGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTg                               >  2:911809/1‑140 (MQ=255)                                                                                                              gggTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGc                              >  1:334026/1‑140 (MQ=255)                                                                                                               ggTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCa                             >  2:214938/1‑140 (MQ=255)                                                                                                               ggTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCa                             >  1:467713/1‑140 (MQ=255)                                                                                                               ggTTACAGGTAAATTCCAGGAAA‑AAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCa                             <  1:1835701/140‑1 (MQ=255)                                                                                                                gTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCaa                            >  1:1705311/1‑140 (MQ=255)                                                                                                                gTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCaa                            >  1:2255340/1‑140 (MQ=255)                                                                                                                   aCAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTc                         >  2:412273/1‑140 (MQ=255)                                                                                                                   aCAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTc                         >  1:1022117/1‑140 (MQ=255)                                                                                                                   aCAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTc                         >  1:792442/1‑140 (MQ=255)                                                                                                                   aCAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTc                         >  1:458360/1‑140 (MQ=255)                                                                                                                    cAGGTAAATTCCAGGCAG‑AAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCa                        <  1:1267756/140‑1 (MQ=255)                                                                                                                    cAGGAAAATTCCAGGAAA‑AAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCa                        <  1:469225/140‑1 (MQ=255)                                                                                                                    aaGGTAAATCCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCa                        <  2:386662/139‑1 (MQ=255)                                                                                                                         aaaTTCCAGGCAG‑AAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTg                   <  2:1422183/140‑1 (MQ=255)                                                                                                                         aaaTTCCAGGCAG‑AAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTg                   <  1:282633/140‑1 (MQ=255)                                                                                                                         aaaTTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTg                   >  1:919213/1‑140 (MQ=255)                                                                                                                           aTTCCAGGCAA‑AAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTa                 <  1:2211757/140‑1 (MQ=255)                                                                                                                              ccAGGAAA‑AAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGc              <  2:1177727/140‑1 (MQ=255)                                                                                                                              ccAGGAAA‑AAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGc              <  1:551616/140‑1 (MQ=255)                                                                                                                               cAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTg                   <  1:704593/134‑1 (MQ=255)                                                                                                                               cAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTg                   >  2:704593/1‑134 (MQ=255)                                                                                                                                 ggCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTg           >  2:1253413/1‑140 (MQ=255)                                                                                                                                    aaaaaaaaac‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGAt         <  2:998185/137‑1 (MQ=255)                                                                                                                                       aaaaaaa‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAag      >  1:252872/1‑140 (MQ=255)                                                                                                                                       aaaaaaa‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAag      >  2:315643/1‑140 (MQ=255)                                                                                                                                       aaaaaaa‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAag      >  2:280564/1‑140 (MQ=255)                                                                                                                                         aaaac‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcg    <  2:1705311/140‑1 (MQ=255)                                                                                                                                           aaa‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcgca  <  2:743277/140‑1 (MQ=255)                                                                                                                                             |                                                                                                                                           AGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGGTTATTTGGGGGTTACAGGTAAATTCCAGGCAG‑AAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCA  >  NC_000913/2001779‑2002055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑