Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,604,3440CT41.6% 144.0 / 94.9 137S62F (TCT→TTT) bglJDNA‑binding transcriptional regulator BglJ
Reads supporting (aligned to +/- strand):  ref base C (14/66);  new base T (57/0);  total (71/66)
Fisher's exact test for biased strand distribution p-value = 1.48e-25
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 161 total aligned reads displayed.

ATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGA‑CTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCA  >  NC_000913/4604207‑4604483
                                                                                                                                         |                                                                                                                                            
aTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttttt                                                                                                                                            >  1:931157/1‑140 (MQ=255)
aTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttctt                                                                                                                                            >  1:266914/1‑140 (MQ=255)
aTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttctt                                                                                                                                            >  2:2273825/1‑140 (MQ=255)
aTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCAATGTCGCGGATCTCGTTTGCGGCGGTTATtttttttt                                                                                                                                            >  2:563363/1‑140 (MQ=255)
  gCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTtttttctttt                                                                                                                                          >  1:1025728/1‑140 (MQ=255)
  gCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttttttt                                                                                                                                          >  1:1657927/1‑140 (MQ=255)
  gCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttctttt                                                                                                                                          >  2:619079/1‑140 (MQ=255)
  gCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttctttt                                                                                                                                          >  1:680135/1‑140 (MQ=255)
     tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTttttctttttct                                                                                                                                       <  2:1126412/140‑1 (MQ=255)
     tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTttttctttttct                                                                                                                                       <  2:1559034/140‑1 (MQ=255)
               taTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCACACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGaa                                                                                                                             >  1:1363452/1‑140 (MQ=255)
                    gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGGAGTGAg                                                                                                                        >  2:166913/1‑140 (MQ=255)
                    gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGGGAg                                                                                                                        >  2:1929261/1‑140 (MQ=255)
                    gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCATGAGAAGTGAg                                                                                                                        >  1:122508/1‑140 (MQ=255)
                    gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAg                                                                                                                        >  1:1586592/1‑140 (MQ=255)
                    gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGGGAg                                                                                                                        >  1:1002060/1‑140 (MQ=255)
                    gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTAAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAg                                                                                                                        >  1:298596/1‑140 (MQ=255)
                    gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCC‑‑ACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGTGAgcg                                                                                                                      >  1:2229956/1‑140 (MQ=255)
                      aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGTGAgcg                                                                                                                      >  1:617055/1‑140 (MQ=255)
                      aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAgcg                                                                                                                      >  1:91377/1‑140 (MQ=255)
                        tGAGAGTTTATTCTGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCa                                                                                                                    <  1:598611/140‑1 (MQ=255)
                        tGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCa                                                                                                                    <  2:2048463/140‑1 (MQ=255)
                        tGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCa                                                                                                                    <  1:108354/140‑1 (MQ=255)
                         gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTCCATGAGAAGTGAGCGCag                                                                                                                   >  2:1371576/1‑140 (MQ=255)
                         gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGTGAGGGCag                                                                                                                   >  2:84159/1‑140 (MQ=255)
                          agagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCaga                                                                                                                  <  2:872591/140‑1 (MQ=255)
                          agagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCaga                                                                                                                  <  1:535273/140‑1 (MQ=255)
                           gagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagag                                                                                                                 <  2:760066/140‑1 (MQ=255)
                           gagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagag                                                                                                                 <  1:619079/140‑1 (MQ=255)
                             gTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGTGAGCGCagagag                                                                                                               >  1:1607074/1‑140 (MQ=255)
                               ttATTCAGAAAGTTTGCGGGTAACCCTTTTAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGTGAGCGCAGAGAggg                                                                                                             >  2:2163852/1‑140 (MQ=255)
                               ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGTGAGGGCAGAGAggg                                                                                                             >  2:1398891/1‑140 (MQ=255)
                               ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCATGAGAAGTGAGCGCAGAGAggg                                                                                                             >  1:1233111/1‑140 (MQ=255)
                               ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAggg                                                                                                             <  2:1302217/140‑1 (MQ=255)
                               ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAggg                                                                                                             <  1:2273825/140‑1 (MQ=255)
                                 aTTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTGGCATTGGAAATGAGCGCAGAGAggggt                                                                                                           >  2:2024641/1‑138 (MQ=255)
                                   tCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTGCCCTTTGAAGTGAGCGCAGAGAgggggtt                                                                                                         >  2:390984/1‑136 (MQ=255)
                                     aGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATc                                                                                                       <  1:2024641/140‑1 (MQ=255)
                                       aaaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTTCCATGAGAAGAGAGGGCAGAGAGGGGTTATCtt                                                                                                     >  2:1170626/1‑140 (MQ=255)
                                       aaaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCtt                                                                                                     >  1:1837732/1‑140 (MQ=255)
                                        aaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTg                                                                                                    <  2:679074/140‑1 (MQ=255)
                                        aaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTg                                                                                                    <  2:2216106/140‑1 (MQ=255)
                                          gTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTTATTTTTTTTTTTTTTGCCCTGAGAAGTGAGCGCCGGGAGGG‑TTATCTTGCct                                                                                                 >  2:605744/1‑140 (MQ=255)
                                             tGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTGCCCTGAGAAGTGAGCGCAGAGAGGGGTTATCTTTCctga                                                                                               >  2:187504/1‑140 (MQ=255)
                                                   tAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGA‑CTGAAc                                                                                        <  2:266914/140‑1 (MQ=255)
                                                     aCCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGA‑CTGAACTg                                                                                      >  1:1382005/1‑140 (MQ=255)
                                                         ttATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGA‑CTGAACTGGCGa                                                                                  <  1:563363/140‑1 (MQ=255)
                                                           ataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGGGTATCTTGCCTGA‑CTGAACTGGCGGtt                                                                                >  2:2174835/1‑140 (MQ=255)
                                                               gCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGA‑CTGAACTGGCGATTAAGt                                                                            <  1:390984/140‑1 (MQ=255)
                                                                cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGGGAGGGCAGAGAGGGGTTATCTTGCCTGA‑CTGAACTGGCGATTAAGtt                                                                           >  2:1608572/1‑140 (MQ=255)
                                                                cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGA‑CTGAACTGGCGATTAAGtt                                                                           <  2:1482059/140‑1 (MQ=255)
                                                                 tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGGGAAGGGAGGGGAGGGAGGGGTTATTTTTCCTGG‑CTGAAATGGCGGTTTAGttt                                                                          >  2:299854/1‑140 (MQ=255)
                                                                     tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGGGAAGTGAGCGCAGAGAGGGGTTATCTTGGCTGA‑CTGAACTGGCGATTAAGGTTTcgc                                                                      >  2:618613/1‑140 (MQ=255)
                                                                     tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCATGAGAAGGGGGCGCAGAGAGGGGTTATCTTTTCTGA‑CTGAACTGGGGATTAAGttttccc                                                                      >  2:805008/1‑136 (MQ=255)
                                                                          ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTTATTTTTTTTTTTTTTGCCATTGGAAGGGGGCGCAGAGAGGGGTTTTCTTTCCTGA‑CTGAACTGGCGATTAAGTTTTCCCCTAcc                                                                 >  2:1271523/1‑140 (MQ=255)
                                                                          ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGGGCAGAGAGGGGTTATCTTTCCTGA‑CTGAACTGGCGGTTAAGTTTTCGCGTAcc                                                                 >  2:1096301/1‑140 (MQ=255)
                                                                          ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGA‑CTGAACTGGCGATTAAGTTTCCGCGTAcc                                                                 <  2:122508/140‑1 (MQ=255)
                                                                           taCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGA‑CTGAACTGGCGATTAAGTTTCCGCGTAccc                                                                <  1:266841/140‑1 (MQ=255)
                                                                           taCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGCCATTTTTTTTTTTTTTGCCATGTTGAGTGAGACGAGAGGGGGGTTAATTTGCCTTA‑ATCTACCGGGGATTAAAGTTTCTCGTAcac                                                                >  2:1484419/1‑138 (MQ=255)
                                                                             ccAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGTGAGGGCCGGGAGGGAGTTTCTTTCCTGA‑CTGAACTGGCGATTAAGGTTTCGCGGACCCgg                                                              >  2:666560/1‑140 (MQ=255)
                                                                               aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGA‑CTGAACTGGCGATTAAGTTTCCGCGTACCCGGCg                                                            <  2:1657927/140‑1 (MQ=255)
                                                                                gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGGTTATCTTTCCTGA‑CTGAACTGGCGATTAAGTTTCCGCGGACCCGGCgg                                                           >  2:1150441/1‑139 (MQ=255)
                                                                                gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGGGGGCGCGGAGAGGGGGTTTTTTTCCTGA‑CTGAACTGGGGATTAAGTTTTCGCGTACCCGGCgg                                                           >  2:509817/1‑139 (MQ=255)
                                                                                gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTTCCCTGAGAAGTGAGCGCAGAGAGGGGTTATCTTGCCTGA‑CTGAACTGGCGATTAAGTTTTCGCGTACCCGGCgg                                                           >  2:777194/1‑139 (MQ=255)
                                                                                gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTTGCATGAGAAGAGAGCGCAGGGGGGGGTTAATTTGCCTGC‑CTGAAATGGCGATTAAATTTTCCCGTACCCcgcgg                                                           >  2:2129174/1‑139 (MQ=255)
                                                                                gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGGTTATCTTTCCTGA‑CTGAACTGGGGGTTAAGTTTCCGCGTACCCcgcgg                                                           >  2:381227/1‑139 (MQ=255)
                                                                                 tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTCGCGGCGGTCATTTTTTTTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGA‑CTGAACTGGCGATTAAGTTTCCGCGTACCCGGCggt                                                          >  2:1042637/1‑138 (MQ=255)
                                                                                  cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCTTTTTTTTTTTTTTTGCCCTGAGAAGGGAGGGCAGAGGGGGGGTTTGTTTCCTCACCTAAATTGGGGATAAGGTTTCC‑CGTAccccggggtt                                                         >  2:1551829/1‑133 (MQ=255)
                                                                                  cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTTCCCTGAGAAGTGAGCGCAGAGGGGGGTTATCTTTCCTGA‑CTGAACTGGCGATTAAGTTTTCGCGTACCCCGCGttt                                                         >  2:1796173/1‑140 (MQ=255)
                                                                                  cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCCTGAGAAGGGAGCGCAGAGAGGGATTATCTTGCCTGA‑CTGAAATGGGGAGTTAGTTTTCGCGCACCCCGCGgtt                                                         >  2:1598116/1‑140 (MQ=255)
                                                                                     gAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGGCGAGGGGGGTTATTTTGCCTGA‑CTGAAATGGGGATTTAGTTTTCCCGTAACCGGCGTTTTGt                                                      >  2:220044/1‑140 (MQ=255)
                                                                                          aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGA‑CTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTg                                                 <  1:2254442/140‑1 (MQ=255)
                                                                                           tttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGGGGGGTTATTTTGCCTGA‑CTGAAATTGCGGTTAAGTTTTCGCGGACCCGGCGGTTAGTTTTTGc                                                >  2:1827948/1‑140 (MQ=255)
                                                                                           tttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGGTTATCTTGCCTGA‑CTTAACTTGCGATTAAGTTTTCGCGGACCCCGCGTTTTGTTAtttt                                                >  2:2254442/1‑138 (MQ=255)
                                                                                            ttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGA‑CTGAACTGGCGATTAAGTTTCCGCGCACCCGGCGTTTAGTTATTGCg                                               <  2:91377/140‑1 (MQ=255)
                                                                                              cAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGA‑CTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGga                                             <  1:147556/140‑1 (MQ=255)
                                                                                               aGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGA‑CTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgat                                            <  1:1486926/140‑1 (MQ=255)
                                                                                               aGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGA‑CTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgat                                            <  2:680135/140‑1 (MQ=255)
                                                                                               aGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGA‑CTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgat                                            <  1:74524/140‑1 (MQ=255)
                                                                                                 gATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGA‑CTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatga                                          <  2:668077/140‑1 (MQ=255)
                                                                                                  aTGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGA‑CTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgat                                         <  2:2250271/140‑1 (MQ=255)
                                                                                                  aTGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGA‑CTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgat                                         <  1:1621962/140‑1 (MQ=255)
                                                                                                   tGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGA‑CTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgatg                                        <  2:48275/140‑1 (MQ=255)
                                                                                                    gCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGA‑CTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgatga                                       <  2:2072392/140‑1 (MQ=255)
                                                                                                    gCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGA‑CTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgatga                                       <  1:2163852/140‑1 (MQ=255)
                                                                                                        tGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTTCCATGGGAAGGGAGCGCAGAGGGGGGTTTTCTTGCCTGA‑CTGGACTGGCGATTAAGTTTTCGCGTACCCGGGGGTTTGTTATTTCGGGTGATGATAtt                                   >  2:67435/1‑140 (MQ=255)
                                                                                                        tGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGA‑CTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATAtt                                   <  1:668755/140‑1 (MQ=255)
                                                                                                            gcgGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGA‑CTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAg                               <  1:1140858/140‑1 (MQ=255)
                                                                                                              ggATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGA‑CTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCt                             <  1:2181386/140‑1 (MQ=255)
                                                                                                                    cGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCCTGTGAAGTGAGAGCAGAGAGGGAATATCTTGCCTTA‑ACGAACTGGGGGATAAGATTTCGCGTACCCCGCGTTTTGTTTTTGGCGATGGTGATATTGAAGcgccggtg                       >  2:357992/1‑133 (MQ=255)
                                                                                                                     gTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGA‑CTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGa                      <  2:1363452/140‑1 (MQ=255)
                                                                                                                      tttGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGA‑CTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGAt                     <  1:362013/140‑1 (MQ=255)
                                                                                                                          cggcggTCATTTTTTTTTTTTTTGCCCTGAGAAGTGAGCGCAGAGAGGGGTTATCTTGCCTGA‑CTGAACTGGCGATTAAATTTTCGCGTACCCCGCGGTTAGTTTTTTCGGGTGATGATATTTAAGCTCGGCTGATTTGt                 >  2:1562445/1‑140 (MQ=255)
                                                                                                                            gcggTCATTTTTTTTTTTTTTGCCCTGAGAAGTGAGCGCAGAGAGGGGTTATCTTTCCTGA‑CTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTc               >  2:1779818/1‑140 (MQ=255)
                                                                                                                             cggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGA‑CTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCa              <  1:1263856/140‑1 (MQ=255)
                                                                                                                               gTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGA‑CTGAACTGGCGATTAAGTTTCCGCGTCCCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCAtt            <  2:488261/140‑1 (MQ=255)
                                                                                                                                   ttttttCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGA‑CTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCg        <  1:1154370/140‑1 (MQ=255)
                                                                                                                                       ttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGA‑CTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAt    <  1:1648530/140‑1 (MQ=255)
                                                                                                                                       ttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGA‑CTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAt    <  2:379945/140‑1 (MQ=255)
                                                                                                                                         ctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGA‑CTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCa  <  1:1796173/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                            
ATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGA‑CTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCA  >  NC_000913/4604207‑4604483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑