BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,143,862	T→C	V80A (GTG→GCG)	frwB →	putative PTS enzyme IIB component FrwB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,143,862	0	T	C	100.0%	474.4 / NA	125	V80A (GTG→GCG)	frwB	putative PTS enzyme IIB component FrwB
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (62/63); total (62/63)

Only 100 of 130 total aligned reads displayed.

TGAAAGTAGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGTGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCAAACCGCTTAAGTTCTTCTCCCGCTCGCAAGGGCGGGTTCGCTTTCCCACAGGAGTTCCTCATGA  >  NC_000913/4143727‑4143998

tGAAAGTAGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCgcgcgc                                                                                                                                      >  2:1206123/1‑140 (MQ=255)

tGAAAGTAGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCgcgcgc                                                                                                                                      <  1:1098783/140‑1 (MQ=255)

 gAAAGTAGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATcgcgcgcg                                                                                                                                     <  1:559050/140‑1 (MQ=255)

   aaGTAGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTc                                                                                                                                   <  1:1172074/140‑1 (MQ=255)

        gAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACAt                                                                                                                              <  1:1132747/140‑1 (MQ=255)

        gAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACAt                                                                                                                              <  2:2077444/140‑1 (MQ=255)

          aaCCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCa                                                                                                                            >  2:116755/1‑140 (MQ=255)

           aCCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAg                                                                                                                           <  2:1233693/140‑1 (MQ=255)

           aCCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAg                                                                                                                           <  1:1317045/140‑1 (MQ=255)

             ccAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCg                                                                                                                         <  1:830988/140‑1 (MQ=255)

              cAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGa                                                                                                                        <  2:1402370/140‑1 (MQ=255)

               aGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGAt                                                                                                                       <  1:1008922/140‑1 (MQ=255)

               aGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGAt                                                                                                                       <  2:281620/140‑1 (MQ=255)

                ggggTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATg                                                                                                                      <  2:1490227/140‑1 (MQ=255)

                ggggTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATg                                                                                                                      >  2:1006933/1‑140 (MQ=255)

                ggggTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATg                                                                                                                      >  1:1599742/1‑140 (MQ=255)

                  ggTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCa                                                                                                                    <  2:546699/140‑1 (MQ=255)

                   gTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAg                                                                                                                   >  1:883207/1‑140 (MQ=255)

                       aTTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAg                                                                                                                           >  2:2014135/1‑128 (MQ=255)

                       aTTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTaaa                                                                                                               >  1:224607/1‑140 (MQ=255)

                       aTTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTaaa                                                                                                               >  2:1239789/1‑140 (MQ=255)

                           gTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTAGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGt                                                                                                           >  2:733647/1‑140 (MQ=255)

                             ttGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGCCC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGc                                                                                                         <  2:2176990/139‑1 (MQ=255)

                             cTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACCAAAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTg                                                                                                          >  1:1141678/1‑140 (MQ=255)

                             cTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGc                                                                                                         <  1:1206123/140‑1 (MQ=255)

                             cTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGc                                                                                                         >  1:2023430/1‑140 (MQ=255)

                             cTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGc                                                                                                         >  1:529506/1‑140 (MQ=255)

                               ggAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTg                                                                                                       <  2:157782/140‑1 (MQ=255)

                               ggAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTg                                                                                                       <  2:284035/140‑1 (MQ=255)

                               ggAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTg                                                                                                       <  2:402859/140‑1 (MQ=255)

                                gAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGa                                                                                                      >  1:1331338/1‑140 (MQ=255)

                                     tGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCa                                                                                                 >  1:1958322/1‑140 (MQ=255)

                                     tGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCa                                                                                                 >  2:1187240/1‑140 (MQ=255)

                                     tGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCa                                                                                                 >  1:1366613/1‑140 (MQ=255)

                                      gAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCAt                                                                                                >  1:1407922/1‑140 (MQ=255)

                                       aaCTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCAtt                                                                                               <  1:1298648/140‑1 (MQ=255)

                                         ctgactgCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTAt                                                                                             <  2:1852223/140‑1 (MQ=255)

                                          tgactgCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATg                                                                                            >  1:42910/1‑140 (MQ=255)

                                          tgactgCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATg                                                                                            >  1:793401/1‑140 (MQ=255)

                                           gactgCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGa                                                                                           >  1:1876357/1‑140 (MQ=255)

                                            acagCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAg                                                                                          >  2:766283/4‑140 (MQ=255)

                                             ctgCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGc                                                                                         <  2:1407922/140‑1 (MQ=255)

                                              tgCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCa                                                                                        >  1:437541/1‑140 (MQ=255)

                                              tgCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCa                                                                                        <  2:2719/140‑1 (MQ=255)

                                               gCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGTTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCaa                                                                                       >  1:1702659/1‑140 (MQ=255)

                                                  gAAGATGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAAt                                                                                    <  2:1293447/140‑1 (MQ=255)

                                                      aTGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGaa                                                                                >  2:1964181/1‑140 (MQ=255)

                                                      aTGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGaa                                                                                >  2:878129/1‑140 (MQ=255)

                                                      aTGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGaa                                                                                >  1:1550292/1‑140 (MQ=255)

                                                        gtgGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGc                                                                              <  1:1239789/140‑1 (MQ=255)

                                                          ggCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTc                                                                            >  2:1053443/1‑140 (MQ=255)

                                                            cGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCAc                                                                          <  2:1447247/140‑1 (MQ=255)

                                                            cGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCAc                                                                          <  1:1014344/140‑1 (MQ=255)

                                                            cGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCAc                                                                          <  2:1366613/140‑1 (MQ=255)

                                                             gAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCAcc                                                                         >  1:2095322/1‑140 (MQ=255)

                                                             gAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCAcc                                                                         <  1:366240/140‑1 (MQ=255)

                                                               gcgcTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCACCTg                                                                       >  2:771268/1‑140 (MQ=255)

                                                                  cTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCACCTGgcg                                                                    >  2:957795/1‑140 (MQ=255)

                                                                    gATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCa                                                                  <  2:1884577/140‑1 (MQ=255)

                                                                     atatGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCaa                                                                 <  1:351691/140‑1 (MQ=255)

                                                                      tatGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCaaa                                                                <  1:771268/140‑1 (MQ=255)

                                                                      tatGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCaaa                                                                <  1:2128215/140‑1 (MQ=255)

                                                                       atGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCAAAc                                                               <  1:733647/140‑1 (MQ=255)

                                                                          gTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCAAACCGc                                                            <  2:1627897/140‑1 (MQ=255)

                                                                          gTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCAAACCGc                                                            >  1:1627897/1‑140 (MQ=255)

                                                                               tCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCAAACCGCTTAAg                                                       >  1:2285207/1‑140 (MQ=255)

                                                                               tCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCAAACCGCTTAAg                                                       >  2:1585986/1‑140 (MQ=255)

                                                                               tCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCAAACCGCTTAAg                                                       <  2:1358735/140‑1 (MQ=255)

                                                                               tCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCAAACCGCTTAAg                                                       <  2:529506/140‑1 (MQ=255)

                                                                                   aCC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCAAACCGCTTAAGttct                                                   >  2:1962297/1‑140 (MQ=255)

                                                                                   aCC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCAAACCGCTTAAGttct                                                   >  2:371224/1‑140 (MQ=255)

                                                                                          gATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCAAACCGCTTAAGTTCTTCTccc                                             <  1:1187240/140‑1 (MQ=255)

                                                                                              tCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGGGTCAACATCAGCGATGCAGTACAACGTGCTGCCGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCAAACCGCTTAAGTTCTTCTCCCGCTc                                         >  2:845603/1‑140 (MQ=255)

                                                                                                  cATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCAAACCGCTTAAGTTCTTCTCCCGCTCGCaa                                     >  1:345671/1‑140 (MQ=255)

                                                                                                   aTCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAATCGTGCTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCAAACCGCTTAAGTTCTTCTCCCGCTCGCAAg                                    >  2:575533/1‑140 (MQ=255)

                                                                                                   aTCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCAAACCGCTTAAGTTCTTCTCCCGCTCGCAAg                                    >  1:390669/1‑140 (MQ=255)

                                                                                                     cAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCAAACCGCTTAAGTTCTTCTCCCGCTCGCAAggg                                  >  2:210764/1‑140 (MQ=255)

                                                                                                      aaGTTTGAAGAGCGTTTTGCTGGCAAGACCATGGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCAAACCGCTTAAGTTCTTCTCCCGCTCGCAAGGGc                                 <  2:390669/140‑1 (MQ=255)

                                                                                                        gTTTGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCAAACCGCTTAAGTTCTTCTCCCGCTCGCAAGGGCgg                               >  1:1948781/1‑140 (MQ=255)

                                                                                                           tGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCAAACCGCTTAAGTTCTTCTCCCGCTCGCAAGGGCGGGtt                            <  2:224607/140‑1 (MQ=255)

                                                                                                           tGAAGAGCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCAAACCGCTTAAGTTCTTCTCCCGCTCGCAAGGGCGGGtt                            <  2:2181974/140‑1 (MQ=255)

                                                                                                              agagCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCAAACCGCTTAAGTTCTTCTCCCGCTCGCAAGGGCGGGTTCGc                         <  1:1006933/140‑1 (MQ=255)

                                                                                                              agagCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCAAACCGCTTAAGTTCTTCTCCCGCTCGCAAGGGCGGGTTCGc                         <  1:878129/140‑1 (MQ=255)

                                                                                                                agCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCAAACCGCTTAAGTTCTTCTCCCGCTCGCAAGGGCGGGTTCGCtt                       >  1:18225/1‑140 (MQ=255)

                                                                                                                agCGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCAAACCGCTTAAGTTCTTCTCCCGCTCGCAAGGGCGGGTTCGCtt                       <  2:1876357/140‑1 (MQ=255)

                                                                                                                  cGTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCAAACCGCTTAAGTTCTTCTCCCGCTCGCAAGGGCGGGTTCGCTTTc                     >  1:1531728/1‑140 (MQ=255)

                                                                                                                   gTTTTGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCAAACCGCTTAAGTTCTTCTCCCGCTCGCAAGGGCGGGTTCGCTTTcc                    >  1:1678744/1‑140 (MQ=255)

                                                                                                                    ttttGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCAAACCGCTTAAGTTCTTCTCCCGCTCGCAAGGGCGGGTTCGCTTTccc                   >  1:1020243/1‑140 (MQ=255)

                                                                                                                    ttttGCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCAAACCGCTTAAGTTCTTCTCCCGCTCGCAAGGGCGGGTTCGCTTTccc                   <  1:163644/140‑1 (MQ=255)

                                                                                                                        gCTGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCAAACCGCTTAAGTTCTTCTCCCGCTCGCAAGGGCGGGTTCGCTTTCCCACAg               <  1:2257636/140‑1 (MQ=255)

                                                                                                                          tGGCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCAAACCGCTTAAGTTCTTCTCCCGCTCGCAAGGGCGGGTTCGCTTTCCCACAGGa             >  1:451918/1‑140 (MQ=255)

                                                                                                                           ggCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCAAACCGCTTAAGTTCTTCTCCCGCTCGCAAGGGCGGGTTCGCTTTCCCACAGGAg            <  2:2095322/140‑1 (MQ=255)

                                                                                                                           ggCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCAAACCGCTTAAGTTCTTCTCCCGCTCGCAAGGGCGGGTTCGCTTTCCCACAGGAg            <  1:180840/140‑1 (MQ=255)

                                                                                                                           ggCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCAAACCGCTTAAGTTCTTCTCCCGCTCGCAAGGGCGGGTTCGCTTTCCCACAGGAg            <  2:437541/140‑1 (MQ=255)

                                                                                                                           ggCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCAAACCGCTTAAGTTCTTCTCCCGCTCGCAAGGGCGGGTTCGCTTTCCCACAGGAg            <  1:1426578/140‑1 (MQ=255)

                                                                                                                            gCAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCAAACCGCTTAAGTTCTTCTCCCGCTCGCAAGGGCGGGCTCGCTTTCCCACAGGAGt           >  2:1940943/1‑140 (MQ=255)

                                                                                                                             cAAGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCAAACCGCTTAAGTTCTTCTCCCGCTCGCAAGGGCGGGTTCGCTTTCCCACAGGAGtt          <  1:1835730/140‑1 (MQ=255)

                                                                                                                               aGACCATCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCAAACCGCTTAAGTTCTTCTCCCGCTCGCAAGGGCGGGTTCGCTTTCCCACAGGAGTTcc        >  1:2167601/1‑140 (MQ=255)

                                                                                                                                    aTCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCAAACCGCTTAAGTTCTTCTCCCGCTCGCAAGGGCGGGTTCGCTTTCCCACAGGAGTTCTTCATg   <  2:1550292/140‑1 (MQ=255)

                                                                                                                                     tCGCGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCAAACCGCTTAAGTTCTTCTCCCGCTCGCAAGGGCGGGTTCGCTTTCCCACAGGAGTTCCTCATGa  <  2:2167601/140‑1 (MQ=255)

TGAAAGTAGAAACCCAGGGGTCAATTGGTCTGGAAAATGAACTGACTGCGGAAGATGTGGCGAGCGCTGATATGGTGATTCTGACC‑AAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGTGCGCGTCAACATCAGCGATGCAGTAAAACGTGCTGACGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCAAACCGCTTAAGTTCTTCTCCCGCTCGCAAGGGCGGGTTCGCTTTCCCACAGGAGTTCCTCATGA  >  NC_000913/4143727‑4143998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`