Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,435	0	C	T	28.8%	121.2 / 27.5	66	intergenic (‑179/‑28)	ttdR/ttdA	DNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (3/44);  new base T (19/0);  total (22/44)
Fisher's exact test for biased strand distribution p-value = 8.90e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGT‑AATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAA  >  NC_000913/3206298‑3206574                                                                                                                                           |                                                                                                                                              aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCa                                                                                                                                             >  2:2074999/1‑140 (MQ=255) aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCa                                                                                                                                             >  1:553757/1‑140 (MQ=255)   aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGa                                                                                                                                           >  2:2105642/1‑140 (MQ=255)   aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAAATTTTTCata                                                                                                                                           >  2:603140/1‑138 (MQ=255)     cATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACa                                                                                                                                         <  2:1600251/140‑1 (MQ=255)        tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAaaaaaaa                                                                                                                                      >  2:647119/1‑139 (MQ=255)          ccATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATAc                                                                                                                                    <  1:1818058/140‑1 (MQ=255)            aTTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGC‑AAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACaaa                                                                                                                                 <  2:1512435/140‑1 (MQ=255)                  tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGAcaaaaacaaaaaata                                                                                                                            >  2:756354/1‑137 (MQ=255)                  tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGAc                                                                                                                                          >  1:1433667/1‑126 (MQ=255)                  tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGAc                                                                                                                                          <  2:1433667/126‑1 (MQ=255)                   gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTgg                                                                                                                           <  2:1963880/140‑1 (MQ=255)                     tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                         <  1:603140/140‑1 (MQ=255)                     tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                         <  1:1168008/140‑1 (MQ=255)                     tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                         <  2:1776858/140‑1 (MQ=255)                     gagacagcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                         <  1:2176947/134‑1 (MQ=255)                          cgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGGATTTTTTCACAAAAAAACAAAAAAAGGAG‑GTGcc                                                                                                                   >  1:1007955/1‑140 (MQ=255)                              atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCatga                                                                                                               <  2:160547/140‑1 (MQ=255)                              atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAAATTTTTTAAACAAAAAAAAAAAAAAGAG‑GTGCCatta                                                                                                               >  2:330434/1‑138 (MQ=255)                                  tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAg                                                                                                           <  1:547010/140‑1 (MQ=255)                                  tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAg                                                                                                           <  1:1082035/140‑1 (MQ=255)                                   cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGACAAAAAAAAAAAAATGAG‑TTGCCCATATGAGc                                                                                                          >  2:1011148/1‑140 (MQ=255)                                     ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGa                                                                                                        <  2:17581/140‑1 (MQ=255)                                     ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGa                                                                                                        <  1:980220/140‑1 (MQ=255)                                       cTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGACAAAAAAAAAAAAATGAG‑TTGCCATGATGAGCGgaa                                                                                                      >  2:251104/1‑140 (MQ=255)                                       cTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGaaa                                                                                                      <  1:370942/140‑1 (MQ=255)                                       cTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGaaa                                                                                                      <  1:756354/140‑1 (MQ=255)                                         tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAGAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGACAAAAAAAAAAAATGGGG‑TTGCCATGATGAGCGAAAga                                                                                                    >  1:766629/1‑139 (MQ=255)                                         tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGACAAAAAAAAAAAATGGAG‑GTGGCATGATGAGGGAAAga                                                                                                    >  1:1501234/1‑139 (MQ=255)                                         tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAAATTTTTCAGACAAAAACAAAAAATGGAG‑GTGGCATGATGAGCGAAAga                                                                                                    >  2:1368466/1‑139 (MQ=255)                                          aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGACAAAAAAAAAAAAAGGAG‑TTGCCATTATGAGGGAAAGt‑a                                                                                                  >  2:770910/1‑140 (MQ=255)                                          aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGt‑a                                                                                                  <  1:1248086/140‑1 (MQ=255)                                          aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑GGAATTTTTTCAGAAAAAAAAAAAAAACAGAG‑TTGGCATGGGAAGCGAAAgaa                                                                                                   >  2:1844833/1‑138 (MQ=255)                                           ttttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑GGAATTTTTTCCGACAAAAACAAAAAATTGAG‑GTTCCATGATTAGCGGAAGa‑aa                                                                                                 >  1:1727183/1‑140 (MQ=255)                                            tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGt‑aat                                                                                                <  1:2140096/140‑1 (MQ=255)                                                gcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGT‑AATAAGc                                                                                            <  2:732447/140‑1 (MQ=255)                                                 cgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGT‑AATAAGCa                                                                                           <  2:1514921/140‑1 (MQ=255)                                                  gTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGACAAAAAAAAAAAATTGAG‑GTGCCATGATGAGCGAAAGa‑aataaaaaa                                                                                          >  1:1879974/1‑136 (MQ=255)                                                  gTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGT‑AATAAGCaa                                                                                          <  2:1112790/140‑1 (MQ=255)                                                   ttGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAAACAAATAAAAAAAAATGGG‑TTGGCATGATTAGTGAAAGT‑AAAAAGGaaa                                                                                         >  2:1099228/1‑139 (MQ=255)                                                   ttGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGT‑AATAAGCAAc                                                                                         <  2:977558/140‑1 (MQ=255)                                                   ttGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGT‑AATAAGCAAc                                                                                         <  2:1971988/140‑1 (MQ=255)                                                      aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGT‑AATAAGCAACAgg                                                                                      <  2:412011/140‑1 (MQ=255)                                                       ttCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGT‑AATAAGCAACAGGc                                                                                     <  1:1122190/140‑1 (MQ=255)                                                        tCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGT‑AATAAGCAACAGGCa                                                                                    <  2:553757/140‑1 (MQ=255)                                                        tCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGT‑AATAAGCAACAGGCa                                                                                    <  1:2039994/140‑1 (MQ=255)                                                             aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGT‑AATAAGCAACAGGCAGTGaa                                                                               <  1:647119/140‑1 (MQ=255)                                                              aCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGT‑AATAAGCAACAGGCAGTGAAt                                                                              <  1:1919368/140‑1 (MQ=255)                                                              aCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGT‑AATAAGCAACAGGCAGTGAAt                                                                              <  2:708951/140‑1 (MQ=255)                                                                tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGACAAAAACAAAAAATGGAG‑GTGGCATGATGGGCGAAAGG‑AATAAAAAACAAGCAGTGAaaaa                                                                            >  2:1138063/1‑140 (MQ=255)                                                                tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGACAAAAAAAAAAAATGGAG‑GTGCCATGATGGGGGAAAGG‑AATAAACAAAAAGCAGAGAATaa                                                                            >  2:1420442/1‑140 (MQ=255)                                                                  ttAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTTAGACAAAAACAAAAAATGGAG‑GTGGCATGATGAGGGAAAGT‑AAAAAACAAAAAGCAGAGAATaaat                                                                          >  2:419650/1‑138 (MQ=255)                                                                  ttAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGACAAATACAAAAAATGGAG‑TTGCCATGATGAGCGAAAGT‑AATAAACAACAGGAAGTGAATAAGt                                                                          >  2:1082874/1‑140 (MQ=255)                                                                  ttAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAAACAAAAAAAAAAACTGGAG‑TTGGCCTGATGAGCGAAAGT‑AATAAACAACAAACAGAGaagaaga                                                                          >  2:1707082/1‑139 (MQ=255)                                                                  ttAATTACATATTCACGGTGGCAAAAAATAT‑AAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGACAAAAACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGG‑AATAAACAAAAGGCAGTGAATAAGtt                                                                         >  1:1400766/1‑140 (MQ=255)                                                                   tAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGT‑AATAAGCAACAGGCAGTGAATAAGtt                                                                         <  1:441553/140‑1 (MQ=255)                                                                    aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGT‑AATAAGCAACAGGCAGTGAATAAGTTg                                                                        <  2:848813/140‑1 (MQ=255)                                                                      ttACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGT‑AATAAGCAACAGGCAGTGAATAAGTTGAc                                                                      <  1:1309249/140‑1 (MQ=255)                                                                            atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGGAATTTTTCAAAAAAAAACAAAAAAATGAGATTGCC‑TGATGAGGGAAAGT‑AATAAAAAAAAAGGAGTGAATAAATTGACAgagaa                                                                >  2:1551849/1‑139 (MQ=255)                                                                            atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGT‑AATAAGCAACAGGCAGTGAATAAGTTGACAGAGAt                                                                <  1:890509/140‑1 (MQ=255)                                                                              tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAAATTTTTCAAACAAAAAAAAAAAATGGAG‑GTGCCATGGTGAGGGAAAGG‑AAAAAAAAAAAAGCAGAGAAAAAAATGACAaagaatt                                                              >  2:1490480/1‑137 (MQ=255)                                                                                  ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAAATTTTTCAGACAAATAAAAAAAAAGGGG‑TTGCCATTATGAGCGAAAGA‑AATAAACAAAAGGCAGTGAATAAATTGACAGAGATTGTCGc                                                          >  2:661622/1‑140 (MQ=255)                                                                                   gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGT‑AATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCt                                                         <  1:2074999/140‑1 (MQ=255)                                                                                    tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGGATTTTTTCAGACAAAAAAAAAAAATGGAG‑TTGTCATTATGAGCGGAAGT‑AATAAACAAAAAGCAGTGAAAAAGTTGACAGAGAGTGTCGCTa                                                        >  2:111210/1‑140 (MQ=255)                                                                                    tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTTAATAAAAAAAAAAAACTGGAG‑GTGCCACGATGAGGGAAAGT‑AAAAAACAAAAAGCAGTGAATTAGATGACAAAGATTGTTGCTa                                                        >  2:1035263/1‑140 (MQ=255)                                                                                     ggCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGGATTTTTTTAGACAAAAACAAAAAATGGGG‑GTGGCATGATGGGGGGAAAT‑AAAAAACAAAAAGCAGGGAATAAATTTGAAAAGAATTTCGCTaa                                                       >  2:573863/1‑140 (MQ=255)                                                                                      gCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAAACAAAAAAAAAAAATAGAG‑GTGCCCTGATGAGCGAAAAA‑AATAAACAAAAAGCAGTGAAAAAATTGAAAGAGATTGTCGCTaaa                                                      >  2:911796/1‑139 (MQ=255)                                                                                         aaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGT‑AATAAGCAACAGGCAGTGAATAAGTTGAGAGAGATTGTCGCTAACttt                                                   <  2:366327/140‑1 (MQ=255)                                                                                           aaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGT‑AATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAc                                                 <  1:1876683/140‑1 (MQ=255)                                                                                                      cacaTTTTTAGTGGTAGTTTGTGGCGG‑TAAATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGT‑AATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGAttt                                      <  1:1471697/140‑1 (MQ=255)                                                                                                        caTTTTTAGTGGTAGTTTGTGGCGGCGGAATTTTTCC‑CACAAAAAAAAAAAAAAGAG‑TTGTCATGATGAGGGGAAAG‑AATAAAAAAAAAACAGCGAAAAAAATGACCAAGATTGTCGCCCACATTAACGCCCTGAgtttt                                    >  2:1109692/1‑138 (MQ=255)                                                                                                        caTTTTTAGTGGTAGTTTGTGGCGG‑TGGATTTTTTCACACAAAAACAAAAAATGGGG‑GTGCCATGTTGAGCGAAAATAAAAAAGAAAAAGGAAG‑GAAAAAATTTACAGAGAATGTCGCTAACTTTATCCCCATGATTTCt                                    >  2:1705396/1‑140 (MQ=255)                                                                                                        caTTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGACAAAAAAAAAAAATGGAG‑TGGCCATGATTAGGGAAAGA‑AAAAAAAAAAAAGCAGTGAAAAAATTGACAGAAATTGTTGCTAACTTTTCCGCCATGATTTCt                                    >  2:1893001/1‑140 (MQ=255)                                                                                                        caTTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGT‑AATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCt                                    <  1:2105642/140‑1 (MQ=255)                                                                                                               ggTGGTAGTTTGGGGGGG‑TGAATTTTTCAAGAAAAATAAAAAAACTGGAG‑TTGCCATGATGAGCAAAAGT‑AATAAACAACAGGCAGTGAATAAGTTAACAGAGATTGTCGCTACCTTTACCGCCATGATTTCAACCCGaa                             <  2:1727183/139‑1 (MQ=255)                                                                                                               aGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGT‑AATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGaa                             <  1:1420442/140‑1 (MQ=255)                                                                                                                   gTAGTTTGTGGCGG‑TGAAATTTTTCAGACAAAAAAAAAAAAAGGGG‑GTTTCATGATGAGGGAAAAG‑AAAAAAAAACAAGCAGAGAAAAAATTGACAGAGAATGTTGCTAAATTTTTCCCCCTGATTTTTATCCCAAAGcc                         >  2:540775/1‑140 (MQ=255)                                                                                                                             gCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGT‑AATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtg               <  1:1368466/140‑1 (MQ=255)                                                                                                                              cGG‑TGAATTTTTTCAGACAAAAAAAAAAAATGGAG‑TTGGCATGATGAGGGAAAGG‑AATAAACAAAAAGCAGTGAAAAAATTGACAGAGATTGTCGCTAACTTTTCCCCCATGATTTTTACCCCAATGCCTGATGACgtgg              >  2:1723273/1‑140 (MQ=255)                                                                                                                                g‑TGAATTTTTTCAGACAAATAAAAAAAATGGAG‑GTGGCATGATGAGGGGAAGT‑AATAAACAACAGGCAGGGAATAAGTTGACAGAGAATGTCGCTAACTTTACCGCCATGATTTCTACCCCAATGCCCGATGACgtgggg            >  2:1345715/1‑138 (MQ=255)                                                                                                                                   gAATTTTTCCAGAAAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGT‑AATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa          <  1:911796/140‑1 (MQ=255)                                                                                                                                   aaaTTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCAAAAGT‑AATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa          <  1:573863/139‑1 (MQ=255)                                                                                                                                      tttttCCAGACAAATAAAAAAACTGGAG‑TTGCCATGATGAGCAAAAGT‑AATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaa       <  1:1705396/140‑1 (MQ=255)                                                                                                                                           ccAGACAAATAAAAAAACTGGAT‑TTGCCATGATGAGCAAAAGT‑AATAAGCAACAGGCGGTGAATAAGTTGACAGAGATTGTCCTTATTTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa  <  2:1007955/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                              AAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGT‑AATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAA  >  NC_000913/3206298‑3206574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑