BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,293,106	(G)_7→6	coding (1595/1659 nt)	nrfE →	putative cytochrome c‑type biogenesis protein NrfE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,293,100	0	G	.	100.0%	483.0 / NA	111	coding (1589/1659 nt)	nrfE	putative cytochrome c‑type biogenesis protein NrfE
Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (46/65); total (46/65)

Only 100 of 124 total aligned reads displayed.

AAGCACGCCGTCAACAAATGATGGAACCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTGCGCACGCTCAGGTCGTAGACACCTGGC  >  NC_000913/4292962‑4293235

aaGCACGCCGTCAACAAATGATGGAACCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTgg                                                                                                                                        >  1:75739/1‑140 (MQ=255)

aaGCACGCCGTCAACAAATGATGGAACCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTgg                                                                                                                                        <  1:816086/140‑1 (MQ=255)

 aGCACGCCGTCAACAAATGATGGAACCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTggg                                                                                                                                       <  1:1297452/140‑1 (MQ=255)

     cGCCGTCAACAAATGATGGAACCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTggggggg                                                                                                                                   >  2:470371/1‑140 (MQ=255)

      gCCGTCAACAAATGATGGAACCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTggggggag                                                                                                                                  <  1:1704188/140‑3 (MQ=255)

      gCCGTCAACAAATGATGGAACCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTggggggag                                                                                                                                  <  1:341273/140‑3 (MQ=255)

       ccGTCAACAAATGATGGAACCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTggggggagg                                                                                                                                 >  1:1487915/1‑137 (MQ=255)

             acaaATGATGGAACCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGAttgtt                                                                                                                          >  1:1978162/1‑140 (MQ=255)

                    atgGAACCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGAtt                                                                                                                   >  1:809492/1‑140 (MQ=255)

                    atgGAACCGTCAATTCGCTGGAACGGCATCCATGACAGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGAtt                                                                                                                   >  2:1193483/1‑140 (MQ=255)

                     tgGAACCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTg                                                                                                                  <  2:1612125/140‑1 (MQ=255)

                      ggAACCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGc                                                                                                                 >  1:992355/1‑140 (MQ=255)

                        aaCCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCgg                                                                                                               >  1:327203/1‑140 (MQ=255)

                         aCCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑‑GGGGGAGGATTGTTGATGATTGCGGgc                                                                                                             >  2:1149437/1‑140 (MQ=255)

                           cGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGgcg                                                                                                            <  2:1684440/140‑1 (MQ=255)

                           cGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGgcg                                                                                                            <  1:637971/140‑1 (MQ=255)

                             tCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCa                                                                                                          <  2:310699/140‑1 (MQ=255)

                             tCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCa                                                                                                          >  2:371281/1‑140 (MQ=255)

                             tCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCa                                                                                                          >  1:1970248/1‑140 (MQ=255)

                                         aaCGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGa                                                                                              >  1:51565/1‑140 (MQ=255)

                                           cGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATg                                                                                            <  1:1002473/140‑1 (MQ=255)

                                           cGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATg                                                                                            <  2:374237/140‑1 (MQ=255)

                                               aTCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCgg                                                                                        >  1:1952079/1‑140 (MQ=255)

                                               aTCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCgg                                                                                        <  1:1022685/140‑1 (MQ=255)

                                                  cATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCggggg                                                                                     <  2:1582948/140‑1 (MQ=255)

                                                    tGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGgaa                                                                                   <  1:1116087/140‑1 (MQ=255)

                                                     gACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGgaag                                                                                  <  1:1472174/140‑1 (MQ=255)

                                                       cTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGgaagaa                                                                                <  2:1952079/140‑1 (MQ=255)

                                                        tGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGaagaag                                                                               >  1:1719537/1‑140 (MQ=255)

                                                          gTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGTGGGGGAAGAAgcg                                                                             >  2:1313819/1‑140 (MQ=255)

                                                          gTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAgcg                                                                             >  2:1624390/1‑140 (MQ=255)

                                                          gTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAgcg                                                                             >  2:393247/1‑140 (MQ=255)

                                                          gTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAgcg                                                                             >  1:605124/1‑140 (MQ=255)

                                                            aTGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGcgcg                                                                           <  1:1500514/140‑1 (MQ=255)

                                                            aTGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGcgcg                                                                           <  1:1193483/140‑1 (MQ=255)

                                                            aTGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGcgcg                                                                           <  1:393247/140‑1 (MQ=255)

                                                             tGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGa                                                                          <  2:226599/140‑1 (MQ=255)

                                                             tGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGa                                                                          <  2:230135/140‑1 (MQ=255)

                                                             tGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGa                                                                          <  1:870194/140‑1 (MQ=255)

                                                             tGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGa                                                                          <  1:1746169/140‑1 (MQ=255)

                                                              gCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGAt                                                                         <  2:775730/140‑1 (MQ=255)

                                                                ggTCATGGGGCAGAAAAATGGGCCGGATCCTTACGCTTTTCGTTTGTACGTATAAACCTGTGTGCCATGAATCT‑GGGGGGCGGATTGTTGATGATTGCGGGCGAAATGTTAAGCCGACGGCGGGGGAAGAAGCGCGATGa                                                                       <  2:1751778/140‑1 (MQ=255)

                                                                 gTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGaa                                                                      >  1:911590/1‑140 (MQ=255)

                                                                  tCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAAt                                                                     >  1:310456/1‑140 (MQ=255)

                                                                     tGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATaaa                                                                  >  1:1359396/1‑140 (MQ=255)

                                                                       ggggAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAgg                                                                <  2:327203/140‑1 (MQ=255)

                                                                       ggggAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAgg                                                                <  1:1592240/140‑1 (MQ=255)

                                                                        gggAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAggg                                                               <  2:1451972/140‑1 (MQ=255)

                                                                        gggAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAggg                                                               <  2:598711/140‑1 (MQ=255)

                                                                         ggAGAAAACTGGGTCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGGTCT‑GGGGGGAGGGTTGTTGATGGTTGCGGGCGCATTGTTAAGCGGGTGGCGGGGGAAGAAGCGCGATGAATAAAGGGc                                                              >  1:1030712/1‑140 (MQ=255)

                                                                         ggAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGc                                                              >  2:934510/1‑140 (MQ=255)

                                                                          gagaAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCt                                                             <  2:946791/140‑1 (MQ=255)

                                                                          gagaAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCt                                                             <  1:919139/140‑1 (MQ=255)

                                                                          gagaAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCt                                                             <  2:1970248/140‑1 (MQ=255)

                                                                             aaaaCTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTtct                                                          <  2:1064644/140‑1 (MQ=255)

                                                                             aaaaCTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTtct                                                          <  2:133365/140‑1 (MQ=255)

                                                                               aaCTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCa                                                        <  1:1422068/140‑1 (MQ=255)

                                                                                 cTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACg                                                      >  2:883500/1‑140 (MQ=255)

                                                                                   gggCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGG‑TGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTa                                                   <  1:247088/140‑1 (MQ=255)

                                                                                        ggATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTActgc                                               <  2:1526911/140‑1 (MQ=255)

                                                                                        ggATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTActgc                                               <  1:445116/140‑1 (MQ=255)

                                                                                        ggATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTActgc                                               <  1:823011/140‑1 (MQ=255)

                                                                                        ggATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTActgc                                               <  2:751009/140‑1 (MQ=255)

                                                                                        ggATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTActgc                                               <  2:75739/140‑1 (MQ=255)

                                                                                        cggtCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTActgc                                               <  2:63/137‑1 (MQ=255)

                                                                                         gATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTActgct                                              >  1:987609/1‑140 (MQ=255)

                                                                                          aTCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTActgctg                                             <  1:1132927/140‑1 (MQ=255)

                                                                                          aTCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTActgctg                                             >  1:819698/1‑140 (MQ=255)

                                                                                          aTCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTActgctg                                             <  1:517312/140‑1 (MQ=255)

                                                                                           tCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGt                                            >  2:1235855/1‑140 (MQ=255)

                                                                                             gTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTa                                          <  2:104248/140‑1 (MQ=255)

                                                                                              ttACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTAt                                         >  2:1552126/1‑140 (MQ=255)

                                                                                               tACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTAtt                                        >  1:1776257/1‑140 (MQ=255)

                                                                                               tACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTAtt                                        >  2:252211/1‑140 (MQ=255)

                                                                                                  gCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTAc                                     >  2:949124/1‑140 (MQ=255)

                                                                                                    ttttCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCt                                   >  1:1087145/1‑140 (MQ=255)

                                                                                                      ttCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGt                                 >  1:163621/1‑140 (MQ=255)

                                                                                                      ttCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGt                                 >  1:1361771/1‑140 (MQ=255)

                                                                                                        cGTTTGTATGTACAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGttt                               <  2:1914663/140‑1 (MQ=255)

                                                                                                            tgtatgtaCAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTgcg                           <  1:705824/140‑1 (MQ=255)

                                                                                                            tgtatgtaCAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTgcg                           <  2:809492/140‑1 (MQ=255)

                                                                                                            tgtatgtaCAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTgcg                           <  1:925769/140‑1 (MQ=255)

                                                                                                              tatgtaCAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTGCGCa                         >  1:1567615/1‑140 (MQ=255)

                                                                                                              tatgtaCAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTGCGCa                         <  1:2031302/140‑1 (MQ=255)

                                                                                                                  taCAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTGCGCACGCt                     <  1:1323680/140‑1 (MQ=255)

                                                                                                                    cAAAGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGTGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTGCGCACGCTCa                   >  2:714917/1‑140 (MQ=255)

                                                                                                                     aaaGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTGCGCACGCTCAg                  <  1:778943/140‑1 (MQ=255)

                                                                                                                     aaaGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTGCGCACGCTCAg                  <  1:883500/140‑1 (MQ=255)

                                                                                                                      aaGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTGCGCACGCTCAgg                 <  1:769810/140‑1 (MQ=255)

                                                                                                                       aGCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTGCGCACGCTCAGGt                >  2:568868/1‑140 (MQ=255)

                                                                                                                        gCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTGCGCACGCTCAGGTc               >  2:378200/1‑140 (MQ=255)

                                                                                                                        gCGGTGTGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTGCGCACGCTCAGGTc               >  1:1100809/1‑140 (MQ=255)

                                                                                                                           gtgtGCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTGCGCACGCTCAGGTCGTa            >  1:1585105/1‑140 (MQ=255)

                                                                                                                            tgtgCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTGCGCACGCTCAGGTCGTAg           >  2:1149771/1‑140 (MQ=255)

                                                                                                                            tgtgCGCTGAATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTGCGCACGCTCAGGTCGTAg           >  1:41067/1‑140 (MQ=255)

                                                                                                                             gtgCGCTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTGCGCACGCTCAGGTCGTAGa          <  1:357550/140‑1 (MQ=255)

                                                                                                                             gtgCGCTGGATCT‑‑GGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTGCGCACGCTCAGGTCGTAGac         <  1:1149437/140‑1 (MQ=255)

                                                                                                                                cgcTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTGCGCACGCTCAGGTCGTAGacac       >  2:1507552/1‑140 (MQ=255)

                                                                                                                                 gcTGGATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTGCGCACGCTCAGGTCGTAGACAcc      >  1:318911/1‑140 (MQ=255)

                                                                                                                                     gATCT‑GGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTGCGCACGCTCAGGTCGTAGACACCTGGc  >  2:1311461/1‑140 (MQ=255)

AAGCACGCCGTCAACAAATGATGGAACCGTCAATTCGCTGGAACGGCATCCATGACTGGTATGCGGTCATGGGGGAGAAAACTGGGCCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGGGGGGGAGGATTGTTGATGATTGCGGGCGCATTGTTAAGCGGATGGCGGGGGAAGAAGCGCGATGAATAAAGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTGCGCACGCTCAGGTCGTAGACACCTGGC  >  NC_000913/4292962‑4293235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`