BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,526,997	C→G	A181P (GCA→CCA)	sgcE ←	KpLE2 phage‑like element; putative epimerase SgcE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,526,997	0	C	G	100.0%	477.1 / NA	126	A181P (GCA→CCA)	sgcE	KpLE2 phage‑like element; putative epimerase SgcE
Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (70/56); total (70/56)

Only 100 of 129 total aligned reads displayed.

TTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTACTTTTTGGATTTTCTCGCACATGGAGGGAATAAACCGCTGTCCCTGCCCATCGGGCTCACTGGTCAT  >  NC_000913/4526860‑4527135

ttGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAATAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCa                                                                                                                                          >  1:1391820/1‑140 (MQ=255)

  gATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGt                                                                                                                                        <  2:1785890/140‑1 (MQ=255)

   aTACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGtt                                                                                                                                       >  1:1543974/1‑140 (MQ=255)

      cGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCt                                                                                                                                    <  2:268976/140‑1 (MQ=255)

       ggTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCtg                                                                                                                                   >  2:427232/1‑140 (MQ=255)

       ggTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCtg                                                                                                                                   >  2:1264725/1‑140 (MQ=255)

          cAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGc                                                                                                                                <  2:98324/140‑1 (MQ=255)

                tctGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGc                                                                                                                          >  1:1022803/1‑140 (MQ=255)

                  tGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCa                                                                                                                        >  2:542798/1‑140 (MQ=255)

                  tGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCa                                                                                                                        >  2:1571163/1‑140 (MQ=255)

                  tGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCa                                                                                                                        >  2:1207545/1‑140 (MQ=255)

                  tGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGAGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCa                                                                                                                        >  1:1458940/1‑140 (MQ=255)

                   gTTGGCTCATCAGAAACAATCTCCTCTTACGGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAg                                                                                                                       <  1:271059/140‑1 (MQ=255)

                   gTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAg                                                                                                                       <  2:1378722/140‑1 (MQ=255)

                       gCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGcatc                                                                                                                   >  2:459202/1‑137 (MQ=255)

                       gCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTa                                                                                                                   <  2:200830/140‑1 (MQ=255)

                       gCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTa                                                                                                                   >  1:177749/1‑140 (MQ=255)

                       gCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTa                                                                                                                   >  1:1734927/1‑140 (MQ=255)

                       gCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTa                                                                                                                   >  1:771897/1‑140 (MQ=255)

                       gCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTa                                                                                                                   >  2:272526/1‑140 (MQ=255)

                       gCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTa                                                                                                                   >  1:727800/1‑140 (MQ=255)

                       gCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAATCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTa                                                                                                                   >  1:812503/1‑140 (MQ=255)

                            tcaGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTcc                                                                                                              >  1:348675/1‑140 (MQ=255)

                            tcaGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTcc                                                                                                              >  1:1510340/1‑140 (MQ=255)

                                aaaCAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCg                                                                                                          >  1:1809039/1‑140 (MQ=255)

                                 aaCAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGt                                                                                                         <  1:1083001/140‑1 (MQ=255)

                                 aaCAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGt                                                                                                         <  1:275277/140‑1 (MQ=255)

                                 aaCAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGt                                                                                                         >  1:292346/1‑140 (MQ=255)

                                 aaCAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGt                                                                                                         <  1:427232/140‑1 (MQ=255)

                                 aaCAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGt                                                                                                         <  2:2005442/140‑1 (MQ=255)

                                 aaCAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGt                                                                                                         >  1:982952/1‑140 (MQ=255)

                                   cAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCg                                                                                                       <  1:1175970/140‑1 (MQ=255)

                                     aTCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGc                                                                                                     >  1:1163657/1‑140 (MQ=255)

                                       ctcctcTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGccc                                                                                                   <  1:1271571/140‑1 (MQ=255)

                                             ttACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACt                                                                                             <  1:806584/140‑1 (MQ=255)

                                             ttACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACt                                                                                             <  1:296685/140‑1 (MQ=255)

                                             ttACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACt                                                                                             <  2:960240/140‑1 (MQ=255)

                                                cAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCAGCCCAGCACTCgg                                                                                          >  2:1198518/1‑140 (MQ=255)

                                                  gggTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTc                                                                                        <  1:434257/140‑1 (MQ=255)

                                                  gggTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTc                                                                                        >  1:1135355/1‑140 (MQ=255)

                                                  gggTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTc                                                                                        <  1:722997/140‑1 (MQ=255)

                                                  gggTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTc                                                                                        >  2:1844629/1‑140 (MQ=255)

                                                   ggTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCt                                                                                       >  1:1580589/1‑140 (MQ=255)

                                                    gTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTg                                                                                      >  2:931541/1‑140 (MQ=255)

                                                    gTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTg                                                                                      >  2:1669038/1‑140 (MQ=255)

                                                    gTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTg                                                                                      >  2:2196/1‑140 (MQ=255)

                                                    gTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTg                                                                                      >  1:1042704/1‑140 (MQ=255)

                                                     tGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGa                                                                                     >  2:1742173/1‑140 (MQ=255)

                                                       gCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAgg                                                                                   >  1:323192/1‑140 (MQ=255)

                                                       gCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAgg                                                                                   >  2:1071299/1‑140 (MQ=255)

                                                        cGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGa                                                                                  >  1:1319431/1‑140 (MQ=255)

                                                                  tAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGc                                                                        <  1:465041/140‑1 (MQ=255)

                                                                   aaGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCg                                                                       <  1:511764/140‑1 (MQ=255)

                                                                     ggTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGATGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTa                                                                     <  1:349451/140‑1 (MQ=255)

                                                                         gcgcgATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTACttt                                                                 >  1:1255447/1‑140 (MQ=255)

                                                                           gcgATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTACttttt                                                               >  1:74428/1‑140 (MQ=255)

                                                                                 gTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTACTTTTTGGAttt                                                         >  1:190265/1‑140 (MQ=255)

                                                                                  tCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTACTTTTTGGAtttt                                                        <  1:995584/140‑1 (MQ=255)

                                                                                  tCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTACTTTTTGGAtttt                                                        <  2:1809039/140‑1 (MQ=255)

                                                                                    aGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTACTTTTTGGATTTtct                                                      <  2:1734927/140‑1 (MQ=255)

                                                                                         aaGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTACTTTTTGGATTTTCTCGcac                                                 <  1:542798/140‑1 (MQ=255)

                                                                                            agaACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTACTTTTTGGATTTTCTCGCACATg                                              >  1:1458977/1‑140 (MQ=255)

                                                                                             gaACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTACTTTTTGGATTTTCTCGCACATgg                                             <  1:1231513/140‑1 (MQ=255)

                                                                                              aaCAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTACTTTTTGGATTTTCTCGCACATGGa                                            <  2:1238462/140‑1 (MQ=255)

                                                                                              aaCAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTACTTTTTGGATTTTCTCGCACATGGa                                            <  2:792743/140‑1 (MQ=255)

                                                                                              aaCAAGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTACTTTTTGGATTTTCTCGCACATGGa                                            <  1:866172/140‑1 (MQ=255)

                                                                                                cAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTACTTTTTGGATTTTCTCGCACATGGAgg                                          >  2:552535/1‑140 (MQ=255)

                                                                                                  gggCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAACACTCGGTCTGAGGAAAAGCCGTGCGTACTTTTTGGATTTTCTCGCACATGGAGGGa                                        >  1:1126833/1‑140 (MQ=255)

                                                                                                   ggCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTACTTTTTGGATTTTCTCGCACATGGAGGGaa                                       >  1:916843/1‑140 (MQ=255)

                                                                                                   ggCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTACTTTTTGGATTTTCTCGCACATGGAGGGaa                                       >  2:705657/1‑140 (MQ=255)

                                                                                                    gcgcgcCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTACTTTTTGGATTTTCTCGCACATGGAGGGAAt                                      <  2:1274934/140‑1 (MQ=255)

                                                                                                     cgcgcCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTACTTTTTGGATTTTCTCGCACATGGAGGGAATa                                     >  1:1915386/1‑140 (MQ=255)

                                                                                                     cgcgcCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTACTTTTTGGATTTTCTCGCACATGGAGGGAATa                                     >  1:1574064/1‑140 (MQ=255)

                                                                                                      gcgcCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTACTTTTTGGATTTTCTCGCACATGGAGGGAATaa                                    <  2:771897/140‑1 (MQ=255)

                                                                                                      gcgcCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTACTTTTTGGATTTTCTCGCACATGGAGGGAATaa                                    <  1:550290/140‑1 (MQ=255)

                                                                                                      gcgcCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTACTTTTTGGATTTTCTCGCACATGGAGGGAATaa                                    <  1:1829350/140‑1 (MQ=255)

                                                                                                      gcgcCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTACTTTTTGGATTTTCTCGCACATGGAGGGAATaa                                    <  2:362753/140‑1 (MQ=255)

                                                                                                        gcCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTACTTTTTGGATTTTCTCGCACATGGAGGGAATAAAc                                  >  1:776002/1‑140 (MQ=255)

                                                                                                        gcCCAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTACTTTTTGGATTTTCTCGCACATGGAGGGAATAAAc                                  >  2:534078/1‑140 (MQ=255)

                                                                                                          ccAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTACTTTTTGGATTTTCTCGCACATGGAGGGAATAAACCg                                <  2:436030/140‑1 (MQ=255)

                                                                                                          ccAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTACTTTTTGGATTTTCTCGCACATGGAGGGAATAAACCg                                <  1:1384979/140‑1 (MQ=255)

                                                                                                           cAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTACTTTTTGGATTTTCTCGCACATGGAGGGAATAAACCGc                               <  2:374945/140‑1 (MQ=255)

                                                                                                           cAATCACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTACTTTTTGGATTTTCTCGCACATGGAGGGAATAAACCGc                               <  2:1579589/140‑1 (MQ=255)

                                                                                                               cACCATATGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTACTTTTTGGATTTTCTCGCACATGGAGGGAATAAACCGCTGTc                           <  2:1970644/140‑1 (MQ=255)

                                                                                                                    tatGTTGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTACTTTTTGGATTTTCTCGCACATGGAGGGAATAAACCGCTGTCCCTGc                      >  2:647305/1‑140 (MQ=255)

                                                                                                                        ttGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTACTTTTTGGATTTTCTCGCACATGGAGGGAATAAACCGCTGTCCCTGCCCAt                  <  2:1580589/140‑1 (MQ=255)

                                                                                                                        ttGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTACTTTTTGGATTTTCTCGCACATGGAGGGAATAAACCGCTGTCCCTGCCCAt                  >  1:580729/1‑140 (MQ=255)

                                                                                                                        ttGCGCCCCAGCTACTGGCATTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTACTTTTTGGATTTTCTCGCACATGGAGGGAATAAACCGCTGTCCCTGCCCAt                  >  2:1009811/1‑140 (MQ=255)

                                                                                                                         tGCGCCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTACTTTTTGGATTTTCTCGCACATGGAGGGAATAAACCGCTGTCCCTGCCCATc                 <  1:1219193/140‑1 (MQ=255)

                                                                                                                           cgcCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTACTTTTTGGGTTTTCTCGCACATGGAGGGAATAAACCGCTGTCCCTGCCCATCgg               >  2:582394/1‑140 (MQ=255)

                                                                                                                           cgcCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTACTTTTTGGATTTTCTCGCACATGGAGGGAATAAACCGCTGTCCCTGCCCATCgg               >  2:1895292/1‑140 (MQ=255)

                                                                                                                           cgcCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTACTTTTTGGATTTTCTCGCACATGGAGGGAATAAACCGCTGTCCCTGCCCATCgg               >  1:1928946/1‑140 (MQ=255)

                                                                                                                            gcCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTACTTTTTGGATTTTCTCGCACATGGAGGGAATAAACCGCTGTCCCTGCCCATCggg              <  2:1284028/140‑1 (MQ=255)

                                                                                                                            gcCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTACTTTTTGGATTTTCTCGCACATGGAGGGAATAAACCGCTGTCCCTGCCCATCggg              >  2:135443/1‑140 (MQ=255)

                                                                                                                            gcCCCAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTACTTTTTGGATTTTCTCGCACATGGAGGGAATAAACCGCTGTCCCTGCCCATCggg              >  2:1219754/1‑140 (MQ=255)

                                                                                                                              cccAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTACTTTTTGGATTTTCTCGCACATGGAGGGAATAAACCGCTGTCCCTGCCCATCGGGCt            <  2:820486/140‑1 (MQ=255)

                                                                                                                                cAGCTGCTGGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTACTTTTTGGATTTTCTCGCACATGGAGGGAATAAACCGCTGTCCCTGCCCATCGGGCTCa          >  2:1069804/1‑140 (MQ=255)

                                                                                                                                   ctgctgGCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTACTTTTTGGATTTTCTCGCACATGGAGGGAATAAACCGCTGTCCCTGCCCATCGGGCTCACTg       <  2:190265/140‑1 (MQ=255)

                                                                                                                                     gctggCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTACTTTTTGGATTTTCTCGCACATGGAGGGAATAAACCGCTGTCCCTGCCCATCGGGCTCACTGGt     <  1:1611624/140‑1 (MQ=255)

                                                                                                                                      ctggCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTACTTTTTGGATTTTCTCGCACATGGAGGGAATAAACCGCTGTCCCTGCCCATCGGGCTCACTGGtc    <  2:292346/140‑1 (MQ=255)

TTGATACGGTCAGGTCTCTGTTGGCTCATCAGAAACAATCTCCTCTTACAGGGTGGCGAATTGAGCTAAGGTCGCGCGATAGTCAGATGAAGAGAACAGGGCGCGCCCAATCACCATATGTTGCGCCCCAGCTGCTGCCAGTTGCTGTGCAGCTGCCAGCGTAATTCCACCGTCGGCCCAGCACTCGGTCTGAGGAAAAGCCGTGCGTACTTTTTGGATTTTCTCGCACATGGAGGGAATAAACCGCTGTCCCTGCCCATCGGGCTCACTGGTCAT  >  NC_000913/4526860‑4527135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`