BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,074,709	T→A	T163S (ACC→TCC)	fumE ←	fumarase E

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,074,709	0	T	A	100.0%	386.7 / NA	111	T163S (ACC→TCC)	fumE	fumarase E
Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (69/42); total (69/42)

Only 100 of 113 total aligned reads displayed.

GATTTACCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTG  >  NC_000913/3074572‑3074845

gATTTACCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAaggagg                                                                                                                                        <  2:331062/140‑1 (MQ=255)

   ttACCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCg                                                                                                                                     <  2:1294579/140‑1 (MQ=255)

     aCCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGtt                                                                                                                                   >  1:1453352/1‑140 (MQ=255)

           tGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCCCCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGcc                                                                                                                             <  2:209106/140‑1 (MQ=255)

           tGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGcc                                                                                                                             >  2:1573859/1‑140 (MQ=255)

                   cgccAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAg                                                                                                                     >  1:25656/1‑140 (MQ=255)

                     ccAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAgcg                                                                                                                   >  1:583374/1‑140 (MQ=255)

                       aaCATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGcc                                                                                                                 <  2:1048342/140‑1 (MQ=255)

                           tgtgCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGttt                                                                                                             >  2:2010328/1‑140 (MQ=255)

                             tgCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTg                                                                                                           >  1:1132807/1‑140 (MQ=255)

                             tgCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTg                                                                                                           >  1:1242689/1‑140 (MQ=255)

                                agcaACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGAcc                                                                                                        >  1:537404/1‑140 (MQ=255)

                                 gcaACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCACTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCa                                                                                                       <  1:91905/140‑1 (MQ=255)

                                  caACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCAt                                                                                                      >  1:1809048/1‑140 (MQ=255)

                                       gTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGc                                                                                                 >  1:1702603/1‑140 (MQ=255)

                                       gTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGCCGTTACTGCCAGCGTCAGCCCCGTTTTGACCATTCCGc                                                                                                 >  2:1604324/1‑140 (MQ=255)

                                             gTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAAc                                                                                           >  2:1797287/1‑140 (MQ=255)

                                             gTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAAc                                                                                           >  1:852597/1‑140 (MQ=255)

                                              tACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAAcg                                                                                          <  1:736806/140‑1 (MQ=255)

                                              tACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAAcg                                                                                          <  2:1453352/140‑1 (MQ=255)

                                              tACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAAcg                                                                                          <  2:421476/140‑1 (MQ=255)

                                                cATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAAcgcg                                                                                        >  1:1803386/1‑140 (MQ=255)

                                                cATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAAcgcg                                                                                        >  1:399670/1‑140 (MQ=255)

                                                 aTTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACgcgc                                                                                       >  2:1281378/1‑140 (MQ=255)

                                                  ttttCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGcc                                                                                      >  2:1536702/1‑140 (MQ=255)

                                                  ttttCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGcc                                                                                      >  1:366267/1‑140 (MQ=255)

                                                  ttttCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGcc                                                                                      >  2:471069/1‑140 (MQ=255)

                                                  ttttCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGcc                                                                                      >  2:1868870/1‑140 (MQ=255)

                                                  ttttCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGcc                                                                                      >  1:1408248/1‑140 (MQ=255)

                                                    ttCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCctc                                                                                    >  2:487251/1‑140 (MQ=255)

                                                     tCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCctct                                                                                   >  1:931619/1‑140 (MQ=255)

                                                        aTCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGAc                                                                                <  2:1316377/140‑1 (MQ=255)

                                                        aTCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGAc                                                                                <  2:932836/140‑1 (MQ=255)

                                                          ctctTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCa                                                                              >  1:1029599/1‑140 (MQ=255)

                                                          ctctTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCa                                                                              <  1:204849/140‑1 (MQ=255)

                                                           tctTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAg                                                                             <  2:1293812/140‑1 (MQ=255)

                                                           tctTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAg                                                                             <  2:1425220/140‑1 (MQ=255)

                                                           tctTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAg                                                                             <  1:770449/140‑1 (MQ=255)

                                                           tctTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAg                                                                             <  1:1573859/140‑1 (MQ=255)

                                                           tctTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAg                                                                             <  1:1689656/140‑1 (MQ=255)

                                                             ttCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGAc                                                                           >  1:1268556/1‑140 (MQ=255)

                                                                   cTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGga                                                                        >  2:20190/1‑137 (MQ=255)

                                                                   cTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGga                                                                        <  1:20190/137‑1 (MQ=255)

                                                                     ggTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTATAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAAt                                                                   <  1:1414876/140‑1 (MQ=255)

                                                                       tACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCa                                                                 >  2:865401/1‑140 (MQ=255)

                                                                       tACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCa                                                                 >  2:1032443/1‑140 (MQ=255)

                                                                       tACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCa                                                                 >  1:1703349/1‑140 (MQ=255)

                                                                          tgtgCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCg                                                              <  2:514040/140‑1 (MQ=255)

                                                                            tgCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGaa                                                            >  2:469225/1‑140 (MQ=255)

                                                                            tgCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGaa                                                            >  1:1830398/1‑140 (MQ=255)

                                                                            tgCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGaa                                                            >  1:1018110/1‑140 (MQ=255)

                                                                            tgCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGaa                                                            >  2:1253356/1‑140 (MQ=255)

                                                                            tgCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGaa                                                            >  1:511331/1‑140 (MQ=255)

                                                                              ccTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACt                                                          >  1:1826965/1‑140 (MQ=255)

                                                                                tGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTc                                                        <  1:765958/140‑1 (MQ=255)

                                                                                      ttCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCt                                                  >  2:1277486/1‑140 (MQ=255)

                                                                                      ttCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCt                                                  >  1:897089/1‑140 (MQ=255)

                                                                                        cAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCttt                                                >  2:1115225/1‑140 (MQ=255)

                                                                                        cAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCttt                                                >  2:1703583/1‑140 (MQ=255)

                                                                                         aGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGGGAAATCAGCGAACTTCATCGCtttt                                               >  2:1544563/1‑140 (MQ=255)

                                                                                         aGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCtttt                                               >  2:235327/1‑140 (MQ=255)

                                                                                          gCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTa                                              <  1:1290504/140‑1 (MQ=255)

                                                                                          gCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTa                                              <  1:511927/140‑1 (MQ=255)

                                                                                          gCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTa                                              >  2:1804892/1‑140 (MQ=255)

                                                                                           cccATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTaa                                             <  2:1306118/140‑1 (MQ=255)

                                                                                           cccATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTaa                                             <  2:543388/140‑1 (MQ=255)

                                                                                           cccATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTaa                                             >  1:1909379/1‑140 (MQ=255)

                                                                                             cATTCACCGTTAATTTAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAAcg                                           <  1:329485/140‑1 (MQ=255)

                                                                                             cATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAAcg                                           <  2:1132807/140‑1 (MQ=255)

                                                                                             cATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAAcg                                           <  2:1242689/140‑1 (MQ=255)

                                                                                               ttCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAAcgcg                                         >  2:729806/1‑140 (MQ=255)

                                                                                               ttCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAAcgcg                                         >  1:576132/1‑140 (MQ=255)

                                                                                               ttCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAAcgcg                                         >  1:10607/1‑140 (MQ=255)

                                                                                                tCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGt                                        >  2:1251456/1‑140 (MQ=255)

                                                                                                   ccGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCAt                                     >  2:1422791/1‑140 (MQ=255)

                                                                                                   ccGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCAt                                     >  1:1080750/1‑140 (MQ=255)

                                                                                                   ccGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGAGAACTTCATCGCTTTTAACGCGTCAt                                     >  1:490140/1‑140 (MQ=255)

                                                                                                    cGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATa                                    <  2:1360650/140‑1 (MQ=255)

                                                                                                     gTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATag                                   >  2:1036581/1‑140 (MQ=255)

                                                                                                     gTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATag                                   >  2:477877/1‑140 (MQ=255)

                                                                                                        aaTTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGc                                >  2:1505201/1‑140 (MQ=255)

                                                                                                         aTTCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCa                               <  1:501032/140‑1 (MQ=255)

                                                                                                          ttCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCAt                              >  2:1687854/1‑140 (MQ=255)

                                                                                                          ttCAATTTTCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCAt                              <  2:366267/140‑1 (MQ=255)

                                                                                                                tttCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCAtt                        <  2:460605/140‑1 (MQ=255)

                                                                                                                tttCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCAtt                        <  1:833163/140‑1 (MQ=255)

                                                                                                                tttCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCAtt                        <  2:1702603/140‑1 (MQ=255)

                                                                                                                  tCACGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGc                      <  1:1286209/140‑1 (MQ=255)

                                                                                                                    aCGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCgg                    >  1:1843756/1‑140 (MQ=255)

                                                                                                                     cGGCGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCggg                   <  2:1028568/140‑1 (MQ=255)

                                                                                                                        cGTTAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTa                <  1:963846/140‑1 (MQ=255)

                                                                                                                          ttAATTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATg              >  2:27745/1‑140 (MQ=255)

                                                                                                                            aaTTCCTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCt            <  2:1830398/140‑1 (MQ=255)

                                                                                                                                 cTTTAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGa       >  2:894078/1‑140 (MQ=255)

                                                                                                                                  tttAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGAc      >  1:835511/1‑140 (MQ=255)

                                                                                                                                  tttAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGAc      >  2:1256434/1‑140 (MQ=255)

                                                                                                                                   ttAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACg     <  1:1136240/140‑1 (MQ=255)

                                                                                                                                   ttAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACg     >  1:1177325/1‑140 (MQ=255)

                                                                                                                                    tAAGGAGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGt    >  2:587095/1‑140 (MQ=255)

                                                                                                                                      aggaggTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTg  <  1:1115225/140‑1 (MQ=255)

GATTTACCGTCTGTAAGGCCGCCAACATGTGCAGCAACGGTTTATGTACATTTTCAATCTCTTCGTTCTGGTACTGTGCCTGTATCTTCAGCCCATTCACCGTTAATTCAATTTTCACGGCGTTAATTCCTTTAAGGTGGTCGTTACTGCCAGCGTCAGCGCCGTTTTGACCATTCCGCTAAAACGCGCCTCTGACCAGACGGAGAAATCAGCGAACTTCATCGCTTTTAACGCGTCATAGAGCATCGCATTGCGGGTTATGCTATTGACGTTG  >  NC_000913/3074572‑3074845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`