Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	501,575	0	A	C	22.7%	91.9 / 11.9	44	V555G (GTG→GGG)	ybaL	putative transporter YbaL
Reads supporting (aligned to +/- strand):  ref base A (33/1);  new base C (0/10);  total (33/11)
Fisher's exact test for biased strand distribution p-value = 4.43e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAAT  >  NC_000913/501436‑501708                                                                                                                                            |                                                                                                                                      gtcgGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTcacca                                                                                                                                       <  2:1714816/140‑1 (MQ=255) gtcgGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTcacca                                                                                                                                       >  2:1046531/1‑140 (MQ=255)   cgGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCaccacc                                                                                                                                     >  1:672937/1‑140 (MQ=255)         gTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCAccc                                                                                                                               >  1:86856/1‑140 (MQ=255)                  aTAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGc                                                                                                                      >  1:383084/1‑140 (MQ=255)                  aTAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGc                                                                                                                      >  1:4276/1‑140 (MQ=255)                  aTAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGc                                                                                                                      >  1:1717689/1‑140 (MQ=255)                  aTAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGc                                                                                                                      >  2:358243/1‑140 (MQ=255)                       gCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTc                                                                                                                 >  1:1007801/1‑140 (MQ=255)                        cGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTcc                                                                                                                >  1:1387075/1‑140 (MQ=255)                           tCACGCCGCATCTGGCTTTTGGCTCTCGATGCCTGATGCGACGCTAGCGCGTCTTATCATGCCTACATATTTTTCATATTTTCCATCCGGCAACCCCCGTTTCCCCCGTCCCCACCTCCCCCGCCGGTGGCGTTTCcagc                                                                                                             <  1:740527/140‑1 (MQ=38)                            cACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCcagca                                                                                                            >  1:545103/1‑140 (MQ=255)                             ccgccgcATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATTTTTTTCATATTTTACATCCGGCAACCCCCGTTTCCCCCGTCACCCCCTCACCCGCCGGTGGCGTTTCcagcag                                                                                                           <  1:1532257/139‑1 (MQ=255)                                ccgcATCTGGCTTTTGCCTCTCGATGCCTGGTGCGCCGCGGGCGCGTTTTATCATGCCTCCATTTTTTTCATTTTTTACACCCGGCACCCCCCTTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTc                                                                                                        <  2:762909/140‑1 (MQ=255)                                ccgcATCTGCTATTTGGCTCTCGATGCCTGATGCGCCGCTGGCGCTTCTTATCATCCCTACATTTTTTTCATTTTTTACACCCGGCAACCCCCTTTTCCCCCGCCACCCCCTCACCCGCCGGTGGCGTTTCCAGCAGTTc                                                                                                        <  2:809879/140‑1 (MQ=255)                                 cgcATCTGGCTTTTGGCTCTCGATGCCTGATGCGCCGCTGGCGCTTTTTATCATGCCTAAATTTTTTTCATTTTTTACACCCGGCAACCACCGTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTcc                                                                                                       <  2:133516/140‑1 (MQ=255)                                 cgcATCTGGCTTTTGGCTCTCGATGCCTGATGCGCCGCTGGCGCGTCTTATCATGCCTACATTTTTTTCATTTTTTACACCCGGCAACCACCGTTTCCCCCGCCCCCCCCTCACCCGCCGGTGGCGTTTCCAGCAGTTcc                                                                                                       <  2:877108/140‑1 (MQ=38)                                 cgcATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTcc                                                                                                       >  1:185406/1‑140 (MQ=255)                                 cgcATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTcc                                                                                                       >  2:1268457/1‑140 (MQ=255)                                     ttagtattttGCCTTTAGATCCCTGATCCGGCGAAAGCGCTTCTTTCCATCCTTACATTTTTTAAATCTTTTCACCCCGGCACCCACCTTATCCCCCGCCCCCCCCCCCCCCGCCGGTAGCGTTTCCAGCAGTTCCAGCa                                                                                                   <  2:1661745/133‑1 (MQ=255)                                                  tctcGTTGCCTGATGCGACGCGGGGGCGTCTTATCATGCCTACATTTTTTTCATATTTTACCCCCGGCACCCCCCGTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGAt                                                                                      <  2:625564/140‑1 (MQ=255)                                                   ctcGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGAtt                                                                                     >  1:155014/1‑140 (MQ=255)                                                   ctcGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGAtt                                                                                     >  2:278389/1‑140 (MQ=255)                                                    tcGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGAttt                                                                                    >  2:1454035/1‑140 (MQ=255)                                                            ggaggcgcCGCGGGCGCTTTTTATCATCCCTAAATTTTTTTCATATTTTCCCTCCGGCACCCCCCTTTTCCCCCCCCCCCCCCCCTCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCg                                                                            <  2:939089/136‑1 (MQ=255)                                                             gatggtaCGCGGGCGCGTTTTATCTTGCTTATTTTTTTTTCATTTTTTACACCCGGCACCCCCCTTTTCCCCCCCCCCCACCCCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGc                                                                           <  2:888555/134‑1 (MQ=255)                                                             gATGCGCCGCGGGCGGGTTTTATCATCCCTACATTTTTTTCATTTTTTACACCCGGCACCCACCTTTTCCCCCGCCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGc                                                                           <  2:1007801/140‑1 (MQ=255)                                                                  gACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATc                                                                      >  2:1068736/1‑140 (MQ=255)                                                                   cggCTGGCGCTTTTTATCATGCCTATATTTTTTTCATTTTTTACCCCCGGCAACCCCCTTTTCCCCCCTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCa                                                                     <  2:1394674/138‑1 (MQ=255)                                                                    cGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCAc                                                                    >  1:1687456/1‑140 (MQ=255)                                                                        ggCGCGTTTTATCATCCCTACATTTTTTTCATATTTTCCACCCGCCAACCCCCGTTTCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTAcc                                                                <  2:1742332/140‑1 (MQ=255)                                                                        ggCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTAcc                                                                >  1:1570854/1‑140 (MQ=255)                                                                            ctttttATCATGCCTACATTTTTTTCATATTTTCCACCCGGCACCCCCCGTTTCCCCTGTCACCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGAt                                                            <  2:1703558/136‑1 (MQ=255)                                                                              tCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTc                                                          >  1:1585953/1‑140 (MQ=255)                                                                              tCTTATCATCCCTACATATTTTTCATATTTTACACCCGCCAACCCCCGTTTCCCCCGTCACCCCCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTc                                                          <  1:1083319/140‑1 (MQ=255)                                                                                  ttCATGCCTACATTTTTTTCATTTTTTCCATCCGGCAACCCCCTTTTCCCCCGTCCCCCCCCCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGcac                                                      <  2:672937/139‑1 (MQ=255)                                                                                   tCATGCCTACATATTTTTCATATTTTTCATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGcacc                                                     >  1:1420960/1‑140 (MQ=255)                                                                                   tCATACCTAAATATTTTTAATATTTTACATCCGGCAACCCCCTTTCCCCCCGCCCCCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGcacc                                                     <  2:1718211/140‑1 (MQ=255)                                                                                      tGCCTACATTTTTTTCATTTTTTACACCCGCCACCCCCCGTTTCCCCCGTCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACg                                                  <  2:1767018/140‑1 (MQ=255)                                                                                       gCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACACTCGCCCATCACTACCTGATTCGCACCACGt                                                 >  1:1145008/1‑140 (MQ=255)                                                                                        ccgaCATTTTTTACATTTTTCTCAGCGGCCAACCGCTGTTTCCCCCCTCCCCACAGCTCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGtt                                                <  2:1037748/137‑1 (MQ=255)                                                                                        ccTACATATTTTTCATTTTTTACATCCGGCACCCCCCTTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGtt                                                <  1:358243/140‑1 (MQ=255)                                                                                        ccTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGtt                                                >  1:1724612/1‑140 (MQ=255)                                                                                           aCATTTTTTTCATTTTTTACCCCCGGCACCCCCCTTTTACCCCGCCCCCCCTCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCgg                                             <  2:1570854/140‑1 (MQ=255)                                                                                           aCATTTTTTTCATATTTTACACCCGCCACCCCCCTTTTCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCgg                                             <  2:1387075/140‑1 (MQ=255)                                                                                               ttttttCATATTTTACACCCGGCAACCCCCGTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGat                                         <  1:1105067/139‑1 (MQ=255)                                                                                                tttttCATATTTTCCACCCGGCACCCCCCGTTTCCCCCGCCCCCCCCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGata                                        <  1:1046531/140‑1 (MQ=255)                                                                                                    tCATATTTTACACCGGGCACCCACCGTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACg                                    <  1:1271212/140‑1 (MQ=255)                                                                                                      atatTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGcc                                  >  2:1557051/1‑140 (MQ=255)                                                                                                      atatTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGcc                                  >  1:1589235/1‑140 (MQ=255)                                                                                                      atatTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGcc                                  >  2:1596745/1‑140 (MQ=255)                                                                                                          tttACACCCGGCAACCCCCGTTTCCCCCGTCCCCCCCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACtt                              <  1:1638369/140‑1 (MQ=255)                                                                                                            ttcacccGGCACCCACCTTTTCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCa                            <  2:1420960/135‑1 (MQ=255)                                                                                                            tccATCCGGCAACCCCCGTTTCCCCCGTCCCCCCCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCa                            <  1:1606056/138‑1 (MQ=255)                                                                                                            tACATCCGTCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCa                            >  2:531602/1‑140 (MQ=255)                                                                                                             acacCCGCCACCCCCCTTTTCCCCCGCCCCCACCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCAt                           <  2:681857/140‑1 (MQ=255)                                                                                                                   ggCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCAt                     >  2:725258/1‑140 (MQ=255)                                                                                                                   ggCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCAt                     >  2:758045/1‑140 (MQ=255)                                                                                                                   ggCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCAt                     >  1:1474048/1‑140 (MQ=255)                                                                                                                    gCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATa                    >  2:260989/1‑140 (MQ=255)                                                                                                                             gTTTCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGcgcg           <  1:490320/140‑1 (MQ=255)                                                                                                                              tttCCCCCGTCCCCCCCTCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCgg          <  2:482202/140‑1 (MQ=255)                                                                                                                              tttACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCgg          >  1:873960/1‑140 (MQ=255)                                                                                                                                  ccccGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCaa      >  1:1686916/1‑140 (MQ=255)                                                                                                                                   cccGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCaat     >  2:826735/1‑140 (MQ=255)                                                                                                                                      gTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCaataat  >  1:320708/1‑140 (MQ=255)                                                                                                                                      gTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCaataat  >  1:541275/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                      GTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAAT  >  NC_000913/501436‑501708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑