Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	286,026	A→T	Q211L (CAG→CTG)	yagG →	putative D‑xylonate transporter YagG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	286,026	0	A	T	100.0%	217.4 / NA	64	Q211L (CAG→CTG)	yagG	putative D‑xylonate transporter YagG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (37/27);  total (37/27)

AGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCAGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGGCGACCTGCTCCAACGTGGTGCGCGGCGGGGCGACGCTCTACTTCGTGAAATA  >  NC_000913/285899‑286161                                                                                                                                |                                                                                                                                        aGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCTGCCGGGCtcgtc                                                                                                                             <  2:1287832/140‑1 (MQ=255)        tcGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCg                                                                                                                      >  2:910758/1‑140 (MQ=255)        tcGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCg                                                                                                                      >  2:738023/1‑140 (MQ=255)        tcGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCg                                                                                                                      >  1:368463/1‑140 (MQ=255)        tcGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCg                                                                                                                      >  1:302013/1‑140 (MQ=255)        tcGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCg                                                                                                                      >  1:791873/1‑140 (MQ=255)        tcGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCg                                                                                                                      >  1:860423/1‑140 (MQ=255)          ggCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCGaa                                                                                                                    <  2:769892/140‑1 (MQ=255)              aaGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCGAAAGAc                                                                                                                >  1:929450/1‑140 (MQ=255)                        gCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAgctgc                                                                                                      >  1:865411/1‑140 (MQ=255)                           ggTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAgctgctgc                                                                                                   <  1:576116/140‑1 (MQ=255)                                      tCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGc                                                                                        >  1:918208/1‑140 (MQ=255)                                      tCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGc                                                                                        >  1:1855810/1‑140 (MQ=255)                                          cgcCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGt                                                                                    >  2:1183186/1‑140 (MQ=255)                                               tgtgCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGgcgc                                                                               >  1:822473/1‑140 (MQ=255)                                               tgtgCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGgcgc                                                                               >  1:681786/1‑140 (MQ=255)                                                 tgcgtgcTGAGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGTTGGGCAACAGCCAGTGGCGcat                                                                             >  2:1670812/1‑140 (MQ=255)                                                           ggCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGcgcg                                                                   <  1:1379767/140‑1 (MQ=255)                                                           ggCTGAGCGGAGTGGTGCTGCTCTACGTCTGCTTCTTCGCGGCCAAAGAGCGCTACCCCTTTGAGGTGGTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGGTGGGCAACAGCCAGTGGCGCATCATGTGcgcg                                                                   <  2:241315/140‑1 (MQ=255)                                                             cTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGtt                                                                 <  1:1038182/140‑1 (MQ=255)                                                             cTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGtt                                                                 <  1:1399634/140‑1 (MQ=255)                                                             cTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCCTCACGACCAAAGAGCGCTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGtt                                                                 >  2:937350/1‑140 (MQ=255)                                                               gAGCGGTGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCa                                                               >  1:1026941/1‑140 (MQ=255)                                                                 gcggagTGGTGCTGCTCTACGTCTGCTTCTACACGGCCAAAGAGCGCTACACTTTGGAGTTGCTGGTGGGCTCGTCGGTGGCGAAAAACCTTAAGCTGCTGCAGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAg                                                             <  2:320318/140‑1 (MQ=255)                                                                  cggcgTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAga                                                            <  1:109810/140‑1 (MQ=255)                                                                  cggcgTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAga                                                            <  1:1255915/140‑1 (MQ=255)                                                                       tggtgCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGatgatg                                                       >  2:609363/1‑140 (MQ=255)                                                                          tgctgcTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGGCg                                                    <  2:302013/140‑1 (MQ=255)                                                                          tgctgcTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGGCg                                                    >  2:869044/1‑140 (MQ=255)                                                                            ctgctCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCTGCCGGGCTGGTCGGTGGGGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGGCGAc                                                  <  2:1894491/140‑1 (MQ=255)                                                                            ctgctCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGGCGAc                                                  <  2:822473/140‑1 (MQ=255)                                                                            ctgctCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGGCGAc                                                  <  2:918208/140‑1 (MQ=255)                                                                             tgctCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGGCGAcc                                                 <  2:1174766/140‑1 (MQ=255)                                                                               ctctACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGGCGACCTg                                               <  2:929450/140‑1 (MQ=255)                                                                               ctctACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGGCGACCTg                                               >  1:120742/1‑140 (MQ=255)                                                                               ctctACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGGCGACCTg                                               <  2:306230/140‑1 (MQ=255)                                                                               ctctACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGGCGACCTg                                               <  1:1744701/140‑1 (MQ=255)                                                                               ctctACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATCTGCGCGTTCAAGATGATGGCGACCTg                                               >  1:1832131/1‑140 (MQ=255)                                                                                    cGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGGCGACCTGCTCCa                                          >  2:903465/1‑140 (MQ=255)                                                                                    cGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGGCGACCTGCTCCa                                          >  2:1826765/1‑140 (MQ=255)                                                                                    cGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCTCCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGGCGACCTGCTCCa                                          >  2:1348178/1‑140 (MQ=255)                                                                                       cTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGGCGACCTGCTCCAACg                                       >  1:114895/1‑140 (MQ=255)                                                                                         gCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGGCGACCTGCTCCAACgtg                                     >  2:1365312/1‑140 (MQ=255)                                                                                            tcttcACGACCAAAGAGCGCTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGGCGACCTGCTCCAACgtggtg                                  <  2:860423/140‑1 (MQ=255)                                                                                              ttcACGACCAAAGAGCGCTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGGCGACCTGCTCCAACGTGGTgcg                                >  1:1189525/1‑140 (MQ=255)                                                                                               tcACGACCAAAGAGCGCTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGGCGACCTGCTCCAACGTGGTgcgc                               >  1:1745812/1‑140 (MQ=255)                                                                                                  cGACCAAAGAGCGCTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGGCGACCTGCTCCAACGTGGTGCgcggc                            >  2:1034716/1‑140 (MQ=255)                                                                                                      cAAAGAGCGCTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGGCGACCTGCTCCAACGTGGTGCGCGGCgggg                        <  1:903465/140‑1 (MQ=255)                                                                                                        aaGAGCGCTACCCCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGGCGACCTGCTCCAACGTGGTGCGCGGCGGGGCg                      <  2:1769103/140‑1 (MQ=255)                                                                                                         agagCGCTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGGCGACCTGCTCCAACGTGGTGCGCGGCGGGGCGa                     <  1:869044/140‑1 (MQ=255)                                                                                                            gcgcTACACCTTTGAGGTGCTGCCGGGCTGGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGGCGACCTGCTCCAACGTGGTGCGCGGCGGGGCGACGc                  <  2:1337655/140‑1 (MQ=255)                                                                                                               cTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGGCGACCTGCTCCAACGTGGTGCGCGGCGGGGCGACGctct               >  2:267014/1‑140 (MQ=255)                                                                                                               cTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGGCGACCTGCTCCAACGTGGTGCGCGGCGGGGCGACGctct               >  1:893168/1‑140 (MQ=255)                                                                                                               cTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGGCGACCTGCTCCAACGTGGTGCGCGGCGGGGCGACGctct               >  1:1112701/1‑140 (MQ=255)                                                                                                               cTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGGCGACCTGCTCCAACGTGGTGCGCGGCGGGGCGACGctct               >  1:671873/1‑140 (MQ=255)                                                                                                               cTACACCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGGCGACCTGCTCCAACGTGGTGCGCGGCGGGGCGACGctct               <  2:791873/140‑1 (MQ=255)                                                                                                                 acacCTTTGAGGTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGGCGACCTGCTCCAACGTGGTGCGCGGCGGGGCGACGCTCTAc             >  1:1377121/1‑140 (MQ=255)                                                                                                                       ttGAGGTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGTGCATCATGTGCGCGTTCAAGATGATGGCGACCTGCTCCAACGTGGTGCGCGGCGGGGCGACGCTCTACTTCGTg       >  2:1682733/1‑140 (MQ=255)                                                                                                                       ttGAGGTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGGCGACCTGCTCCAACGTGGTGCGCGGCGGGGCGACGCTCTACTTCGTg       <  1:479973/140‑1 (MQ=255)                                                                                                                          aGGTGCTGCCGGGCTCGTCGGTGGCGACAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGGCGACCTGCTCCAACGTGCTGCGCGGCGGGGCGACGCTCTACTTCGTGaaa    >  2:1017194/1‑140 (MQ=255)                                                                                                                          aGGTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGGCGACCTGCTCCAACGTGGTGCGCGGCGGGGCGACGCTCTACTTCGTGaaa    >  2:1824716/1‑140 (MQ=255)                                                                                                                           ggTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGGCGACCTGCTCCAACGTGGTGCGCGGCGGGGCGACGCTCTACTTCGTGAAAt   <  1:1166321/140‑1 (MQ=255)                                                                                                                            gTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGGCGACCTGCTCCAACGTGGTGCGCGGCGGGGCGACGCTCTACTTCGTGAAATa  <  2:1745812/140‑1 (MQ=255)                                                                                                                            gTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGGCGACCTGCTCCAACGTGGTGCGCGGCGGGGCGACGCTCTACTTCGTGAAATa  <  1:404885/140‑1 (MQ=255)                                                                                                                            gTGCTGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGGCGACCTGCTCCAACGTGGTGCGCGGCGGGGCGACGCTCTACTTCGTGAAATa  >  1:407601/1‑140 (MQ=255)                                                                                                                                |                                                                                                                                        AGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCTCTACGTCTGCTTCTTCACGACCAAAGAGCGCTACACCTTTGAGGTGCAGCCGGGCTCGTCGGTGGCGAAAGACCTTAAGCTGCTGCTGGGCAACAGCCAGTGGCGCATCATGTGCGCGTTCAAGATGATGGCGACCTGCTCCAACGTGGTGCGCGGCGGGGCGACGCTCTACTTCGTGAAATA  >  NC_000913/285899‑286161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑