Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4350CT32.0% 30.2 / 12.0 25intergenic (‑179/‑28)ttdR/ttdADNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (0/17);  new base T (8/0);  total (8/17)
Fisher's exact test for biased strand distribution p-value = 9.25e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01
Rejected as consensus: Frequency below/above cutoff threshold.

AAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTG  >  NC_000913/3206298‑3206561
                                                                                                                                          |                                                                                                                               
aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCa                                                                                                                               >  1:91130/1‑140 (MQ=255)
  aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑GATTTTTTCAGa                                                                                                                             >  1:818047/1‑140 (MQ=255)
       tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑GATTTTTTCAGACaaaa                                                                                                                        >  1:484343/1‑139 (MQ=255)
       tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAaaaaaaa                                                                                                                        >  1:1247819/1‑139 (MQ=255)
       tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGG‑AAATTTTTCAGACaaaa                                                                                                                        >  2:855336/1‑139 (MQ=255)
          cATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACa                                                                                                                     <  1:1147355/140‑1 (MQ=255)
              cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCG‑GATTTTTTCAGACACAaaaaaaaa                                                                                                                 >  1:49451/1‑140 (MQ=255)
                 tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGTAATTTTTCCAGCCAATTAaaaaaatg                                                                                                               >  2:500892/1‑138 (MQ=255)
                   ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCACACAaaaaaaaaaaattga                                                                                                            >  2:1150436/1‑135 (MQ=255)
                   ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAGACAaaaaaaaaaaaaggg                                                                                                            >  2:819077/1‑135 (MQ=255)
                        gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAGACAAAAACAAAAAATGGAGATgg                                                                                                       >  2:1203669/1‑139 (MQ=255)
                           cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑GAATTTTTCAAACAAAAAAAAAAAATGGAGTTGGCat                                                                                                    >  2:386087/1‑140 (MQ=255)
                           cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTTAGCCAAAAACAAAAACTGGAGTTGCCat                                                                                                    >  2:430307/1‑140 (MQ=255)
                             atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCat‑ga                                                                                                 <  1:430307/140‑1 (MQ=255)
                                 tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAg                                                                                             <  1:531204/140‑1 (MQ=255)
                                  cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCATACAAATAAAAAAAATGGAGTTGCCAT‑GATGAgg                                                                                            >  2:179769/1‑139 (MQ=255)
                                   attcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCg                                                                                           <  2:951268/140‑1 (MQ=255)
                                      cTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAACAAAAAAAAAAAAATGAGTTGGCAT‑GATGAggggaa                                                                                        >  2:598462/1‑135 (MQ=255)
                                        tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAACAAAAAATGGAGGTGCCCT‑GATGGGTGAAAGt                                                                                      >  2:697892/1‑140 (MQ=255)
                                        tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCCGACAAAAAAAAAAAAATGAGGTGCCAT‑TATGAGCGAAAga                                                                                      >  1:121333/1‑139 (MQ=255)
                                           tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGtaat                                                                                   <  2:363968/140‑1 (MQ=255)
                                             tCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAAAAAAAAAATGAGTTGCCAA‑AATGAGGGaaaagaaaaa                                                                                 >  1:811679/1‑133 (MQ=255)
                                                cgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAAAAAAAAAAGGAGGTGGCCT‑GATGAGCGGAAGTAATAAACa                                                                              >  1:521363/1‑140 (MQ=255)
                                                     aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATTAGCGAAAGTAATAAGCAACAgg                                                                         <  2:1081701/140‑1 (MQ=255)
                                                     aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAgg                                                                         <  1:89486/140‑1 (MQ=255)
                                                            aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGaa                                                                  <  1:910185/140‑1 (MQ=255)
                                                            aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGaa                                                                  <  1:179769/140‑1 (MQ=255)
                                                               tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCCGACAAAAAAAAAAAAAAGAATTGGCATAGAT‑AGCGAAAATAATAAACAACAAGCCGGGAATaa                                                               >  2:1068061/1‑140 (MQ=255)
                                                                      tACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACa                                                        <  2:1248601/140‑1 (MQ=255)
                                                                         atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAACAAAAAAAAAAAATGGGGGTTGCAT‑TATGAGCGAAAGTAAAAAACAAAAAGGAGGGAAAAAAGTGACagag                                                     >  2:709639/1‑140 (MQ=255)
                                                                         atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAG‑GATTTTTTCTGACAAATAAAAAAAAAGGGGTTGGCAA‑GATGAGCGAAAAAAAAAAACAACAAGCAGCGGATAAAtttgaaaag                                                     >  2:282332/1‑133 (MQ=255)
                                                                           atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑GATTTTTTCAAAAAAAAAAAAAAAATAGAGTTGCCAT‑GATGATGGAAAATAATAAAAAAAAAGCAAAGAAAAAAGTGACAgagaa                                                   >  2:614577/1‑139 (MQ=255)
                                                                           atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAAAAAAAAATGGAGGTTCCAT‑GGTGAGAGGAAATAATAAGCAACAAGGAGGGAAAAAGTTGGAAGAGAt                                                   >  2:672659/1‑140 (MQ=255)
                                                                           atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGAt                                                   <  1:379403/140‑1 (MQ=255)
                                                                                 ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGc                                             <  1:855336/140‑1 (MQ=255)
                                                                                   tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAAACAAAAAAAAAAAATGGAGGTGGCAT‑GATGGGGGAAAGGAATAAACAAAAAGCCAGGAATAAATTGACAAAGAATTTTGCTa                                           >  2:1065736/1‑140 (MQ=255)
                                                                                     gCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAAAAAAAAAAGGAGGTGGCCT‑GATTGGGGAAAGAAAAAAACAACAAGCAAGGAAAAAAATGAAAAAAAATGTTGCTAAc                                         >  2:132315/1‑140 (MQ=255)
                                                                                               aaaacCCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGTTTGTCGCTAACTTTACCGCCa                               <  2:121333/139‑1 (MQ=255)
                                                                                                       caTTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAAAAAAAAATGGAGTTTTCAT‑GATGAGGGGAAGGAAAAAAAAAAAAGCAGTGAAAAAATTGGCAGAGATTTTCGCTAACTTTATCGCCCTGAttttt                       >  2:1052665/1‑138 (MQ=255)
                                                                                                         tttttAGTGGTAGTTTGTGGCGGTG‑AATTTTTCAAGAAAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTAc                     <  2:678199/140‑1 (MQ=255)
                                                                                                             tAGTGGTATTTTGTGGCGGTG‑AATTTTTCCAGAAAAATAAAAAAACTGGAGTTGCCAT‑GATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGa                 <  1:1203669/140‑1 (MQ=255)
                                                                                                                  gTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGcc            <  1:672659/140‑1 (MQ=255)
                                                                                                                       ttGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtgatg       <  1:598462/140‑1 (MQ=255)
                                                                                                                            gCGGTG‑AATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtg  <  2:411383/140‑1 (MQ=255)
                                                                                                                            gCGGTG‑AATTTTCCCAAACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtg  <  1:806297/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                               
AAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTG  >  NC_000913/3206298‑3206561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑