New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 2518224	79 (0.900)	3 (0.040)	3/242	7.5	5.0%	noncoding (8/76 nt)	alaW	tRNA‑Ala
	?	NC_000913	= 2521057	46 (0.600)					noncoding (7/76 nt)	valX	tRNA‑Val
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CTCCACACAGCGGGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCA‑CCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTTCTCGCCAGCTAAATTTCTTGTAAAAATGTGAAGTACCGAAGTGGGTGATTAGCTCAGCTGGG  >  NC_000913/2520918‑2521192                                                                                                                                             |                                                                                                                                        cTCCACACAGCGGGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCA‑CCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGAt                                                                                                                                         <  2:12162/140‑1 (MQ=255)      cacaGCGGGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTGGATCCCCCGACCA‑ACCACCAATTACTTTTTTTGGTCTCCGCCGTGTAAAAAGAAAATTAGAAGGGGGGGGGTAGCt                                                                                                                                    >  2:174463/1‑140 (MQ=12)      cacaGCGGGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCA‑CCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCt                                                                                                                                    >  2:854563/1‑140 (MQ=255)      cacaGCGGGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCA‑CCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCt                                                                                                                                    <  2:486234/140‑1 (MQ=255)       acaGCGGGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCA‑CCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTc                                                                                                                                   <  2:1383498/140‑1 (MQ=255)              gggTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCCTCCTCA‑CCCACCAACTACTTTATGTCGTCTCCGCCGGGTAGCAAGAAAATGAGAAGAGGGGGATTATCTCAGCTggg                                                                                                                            >  2:338506/1‑140 (MQ=21)                     tAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCA‑CCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCAc                                                                                                                     <  1:630474/140‑1 (MQ=255)                      aGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCAc                                                                                                                     <  2:245698/140‑1 (MQ=255)                       gCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCA‑CCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCt                                                                                                                   <  2:205030/140‑1 (MQ=255)                       gCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCA‑CCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCt                                                                                                                   <  1:213797/140‑1 (MQ=255)                       gCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCA‑CCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCt                                                                                                                   <  1:366761/140‑1 (MQ=255)                       gCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCCGTTCGATCCCGTCATCA‑CCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCt                                                                                                                   <  2:281313/140‑1 (MQ=255)                         tCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCA‑CCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTcc                                                                                                                 <  1:854563/140‑1 (MQ=255)                             cTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCA‑CCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTa                                                                                                             <  2:724048/140‑1 (MQ=255)                                  agagCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCA‑CCCACCAACTTCTTTATGGAGTATCCTCCGGGTAGCAAGAAAATGAGCAGAGGGGGATTATCTCCGCTTGGGGAGCACCCCCCTTACAagg                                                                                                        >  2:293813/1‑140 (MQ=18)                                      cACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCA‑CCCACCAACTACTTTATGTATTCTCCGCCGTGTAGCAAGAAAATGAGAAGTGGGGGATTAGCTCAGCTGGGGGAGCACCCCCCTTACAaagagtg                                                                                                    >  2:1115154/1‑138 (MQ=37)                                       aCCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCA‑CCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAgggg                                                                                                   >  1:1198592/1‑140 (MQ=255)                                            ccTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCA‑CCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTcgg                                                                                              <  2:365554/140‑1 (MQ=255)                                                 cAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCA‑CCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGtt                                                                                         <  2:1230723/140‑1 (MQ=255)                                                 cAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCA‑CCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGtt                                                                                         <  2:1198592/140‑1 (MQ=255)                                                      aggGGGTCGGCGGTTCGATCCCGTCATCA‑CCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATc                                                                                    >  1:1178323/1‑140 (MQ=255)                                                      aggGGGTCGGCGGTTCGATCCCGTCATCA‑CCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATc                                                                                    >  1:421934/1‑140 (MQ=255)                                                        ggggTCGGCGGTTCGATCCCGTCATCA‑CCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATccc                                                                                  >  2:746412/1‑140 (MQ=255)                                                                cggTTCGATCCCGTCATCA‑CCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATcac                                                                          <  2:1178323/140‑1 (MQ=255)                                                                   ttCGATCCCGTCATCA‑CCCACCATCTACTTTATGTAGGCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATcaccca                                                                       <  1:455092/140‑1 (MQ=255)                                                                   ttCGATCCCGTCATCA‑CCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATcaccca                                                                       >  1:42707/1‑140 (MQ=255)                                                                          ccGTCATCA‑CCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACttc                                                                <  1:999030/140‑2 (MQ=255)                                                                                 ca‑cccaccAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGTGGTTCGATCCCGTCATCACCCACCACTTTCTCGCCa                                                         >  2:696114/1‑140 (MQ=255)                                                                                             tactTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTTCTCGCCAGCTAAATTTCt                                              >  2:524239/1‑140 (MQ=255)                                                                                                 ttATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGTGGTTCGATCCCGTCATCACCCACCACTTTCTCGCCAGCTAAATTTCTTGTa                                          <  1:696114/140‑1 (MQ=255)                                                                                                 ttATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTTCTCGCCAGCTAAATTTCTTGTa                                          <  1:1466038/140‑1 (MQ=255)                                                                                                 ttATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTTCTCGCCAGCTAAATTTCTTGTa                                          <  1:463559/140‑1 (MQ=255)                                                                                                   aTGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTTCTCGCCAGCTAAATTTCTTGTaaa                                        >  2:445494/1‑140 (MQ=255)                                                                                                   aTGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTTCTCGCCAGCGAAATTTCTTGTaaa                                        >  2:1328825/1‑140 (MQ=255)                                                                                                    tGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTTCTCGCCAGCTAAATTTCTTGTaaaa                                       <  2:421934/140‑1 (MQ=255)                                                                                                    tGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTTCTCGCCAGCTAAATTTCTTGTaaaa                                       >  2:1087822/1‑140 (MQ=255)                                                                                                       aGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCCCCACTTTCTCGCCAGCTAAATTTCTTGTTAAAAtg                                    >  1:1094697/1‑140 (MQ=255)                                                                                                       aGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTTCTCGCCAGCTAAATTTCTTGTAAAAAtg                                    <  1:746412/140‑1 (MQ=255)                                                                                                       aGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTTCTCGCCAGCTAAATTTCTTGTAAAAAtg                                    >  1:1263099/1‑140 (MQ=255)                                                                                                         tctcCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTTCTCGCCAGCTAAATTTCTTGTAAAAAtgtg                                  <  2:116225/140‑1 (MQ=255)                                                                                                         tctcCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTTCTCGCCAGCTAAATTTCTTGTAAAAAtgtg                                  >  1:459198/1‑140 (MQ=255)                                                                                                         tctcCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTTCTCGCCAGCTAAATTTCTTGTAAAAAtgtg                                  <  1:445494/140‑1 (MQ=255)                                                                                                         tctcCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTTCTCGCCAGCTAAATTTCTTGTAAAAAtgtg                                  <  1:293813/140‑1 (MQ=255)                                                                                                          ctcCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTTCTCGCCAGCTAAATTTCTTGTAAAAATGTGa                                 <  1:1087822/140‑1 (MQ=255)                                                                                                           tcCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTTCTCGCCAGCTAAATTTCTTGTAAAAATGTGaa                                >  1:1407492/1‑140 (MQ=255)                                                                                                                cgTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTTCTCGCCAGCTAAATTTCTTGTAAAAATGTGAAGTAcc                           <  2:1242244/140‑1 (MQ=255)                                                                                                                  tgtAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTTCTCGCCAGCTAAAATTCTTGTAAAAATTTGAAGTACCGa                         >  2:1204582/1‑140 (MQ=255)                                                                                                                     aGCAAGAAATTGAGGAGGGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTTCTCGCCAGCTAAATTTCTTGTAAAAATGTGAAGTACCGAAGt                      <  2:265168/140‑1 (MQ=255)                                                                                                                       cAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTTCTCGCCAGCTAAATTTCTTGTAAAAATGTGAAGTACCGAAGTgg                    <  2:42707/140‑1 (MQ=255)                                                                                                                            aaTTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTTCTCGCCAGCTAAATTTCTTGTAAAAATGTGAAGTACCGAAGTGGGTGAt               >  2:440029/1‑140 (MQ=255)                                                                                                                                gagaAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTTCTCGCCAGCTAAATTTCTTGTAAAAATGTGAAGTACCGAAGTGGGTGATTAGc           <  2:788018/140‑1 (MQ=255)                                                                                                                                         gTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTTCTCGCCAGCTAAATTTCTTGTAAAAATGTGAAGTACCGAAGTGGGTGATTAGCTCAGCTggg  <  2:728801/140‑1 (MQ=255)                                                                                                                                             |                                                                                                                                        CTCCACACAGCGGGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCA‑CCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTTCTCGCCAGCTAAATTTCTTGTAAAAATGTGAAGTACCGAAGTGGGTGATTAGCTCAGCTGGG  >  NC_000913/2520918‑2521192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.