Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	72,956	A→T	F522L (TTT→TTA)	thiP ←	thiamine ABC transporter membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	72,956	0	A	T	100.0%	237.6 / NA	68	F522L (TTT→TTA)	thiP	thiamine ABC transporter membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (35/33);  total (35/33)

CCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCA  >  NC_000913/72819‑73091                                                                                                                                          |                                                                                                                                        ccGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTtaa                                                                                                                                       >  2:186541/1‑140 (MQ=255) ccGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTtaa                                                                                                                                       <  2:47431/140‑1 (MQ=255)  cgcgCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAAc                                                                                                                                      >  1:639774/1‑140 (MQ=255)    cgccgcTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAAcag                                                                                                                                    >  2:125129/1‑140 (MQ=255)    cgccgcTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAAcag                                                                                                                                    >  1:1126687/1‑140 (MQ=255)    cgccgcTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAAcag                                                                                                                                    >  1:1202066/1‑140 (MQ=255)    cgccgcTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAAcag                                                                                                                                    >  1:187077/1‑140 (MQ=255)     gccgcTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAAcagc                                                                                                                                   <  1:284066/140‑1 (MQ=255)     gccgcTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTCTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAAcagc                                                                                                                                   <  1:516114/140‑1 (MQ=255)      ccgcTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAAcagca                                                                                                                                  <  1:2215/140‑1 (MQ=255)                cGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACagag                                                                                                                        <  2:695734/140‑1 (MQ=255)                  aGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGagca                                                                                                                      <  1:1083380/140‑1 (MQ=255)                  aGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGagca                                                                                                                      >  2:1215708/1‑140 (MQ=255)                      tCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGagcagcag                                                                                                                  <  2:554373/140‑1 (MQ=255)                      tCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGagcagcag                                                                                                                  >  2:489377/1‑140 (MQ=255)                             cTGCTCGCCGCGTTCCACCGTTAAGCTAAAACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAAtt                                                                                                           >  2:592984/1‑140 (MQ=255)                              tGCTCGCCGCGTTCCACCGTTAAGCTAAAACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGagcagcag                                                                                                                  >  2:104869/1‑132 (MQ=255)                              tGCTCGCCGCGTTCCACCGTTAAGCTAAAACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGagcagcag                                                                                                                  <  1:104869/132‑1 (MQ=255)                                    ccgcGTTCCACCGTTAAGCTAAAACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCgg                                                                                                    >  2:1000137/1‑140 (MQ=255)                                    ccgcGTTCCACCGTTAAGCTAAAACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCgg                                                                                                    >  2:1245946/1‑140 (MQ=255)                                     cgcgTTCCACCGTTAAGCTAAAACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGt                                                                                                   >  1:57756/1‑140 (MQ=255)                                     cgcgTTCCACCGTTAAGCTAAAACGCATCGGCAAATGGTGGTAAAGCCAGGCGATATCAGTCAGTTTTAACATTTCGCCCCGGAAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGt                                                                                                   >  1:494475/1‑140 (MQ=255)                                       cgTTCCACCGTTAAGCTAAAACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGa                                                                                                 <  2:187077/140‑1 (MQ=255)                                        gTTCCACCGTTAAGCTAAAACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAAATAACGCGGTGAc                                                                                                <  1:238898/140‑1 (MQ=255)                                          tCCACCGTTAAGCTAAAACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCg                                                                                              >  1:195134/1‑140 (MQ=255)                                                 ttAAGCTAAAACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTc                                                                                       >  1:318143/1‑140 (MQ=255)                                                 ttAAGCTAAAACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTc                                                                                       >  1:283895/1‑140 (MQ=255)                                                  tAAGCTAAAACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTcc                                                                                      <  1:186541/140‑1 (MQ=255)                                                   aaGCTAAAACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCt                                                                                     <  2:438853/140‑1 (MQ=255)                                                    aGCTAAAACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTg                                                                                    <  2:1202066/140‑1 (MQ=255)                                                              cATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAg                                                                          <  1:148746/140‑1 (MQ=255)                                                               aTCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAgg                                                                         <  1:1246759/140‑1 (MQ=255)                                                                tCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGa                                                                        >  1:627782/1‑140 (MQ=255)                                                                tCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGa                                                                        <  1:1236347/140‑1 (MQ=255)                                                                   gCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGcc                                                                     >  1:1199464/1‑140 (MQ=255)                                                                     aaaTGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCaa                                                                   <  2:380785/140‑1 (MQ=255)                                                                     aaaTGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCaa                                                                   <  2:318143/140‑1 (MQ=255)                                                                     aaaTGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCaa                                                                   <  2:283895/140‑1 (MQ=255)                                                                       aTGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAAtt                                                                 >  2:483360/1‑140 (MQ=255)                                                                         ggtggtAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTg                                                               <  2:709853/140‑1 (MQ=255)                                                                            ggtAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTg                                                            >  2:93659/1‑140 (MQ=255)                                                                             gtAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTgg                                                           >  1:948575/1‑140 (MQ=255)                                                                             gtAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTgg                                                           >  1:1153314/1‑140 (MQ=255)                                                                              tAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGt                                                          <  2:514689/140‑1 (MQ=255)                                                                                aaGCGAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTGTTCAATCAGGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCAACGTCCTGGCTGCGATAGGAGCCAAGTTGCTGGTag                                                        >  2:980203/1‑140 (MQ=255)                                                                                aaGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTag                                                        <  2:1126687/140‑1 (MQ=255)                                                                                 aGCCAGGTGATATCAGTCGGTTTTAACATTTCGCCCCGGTAGTTTTTCGATCTCGGTTAACAGCAGATAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTaga                                                       <  2:494475/140‑1 (MQ=255)                                                                                 aGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTaga                                                       <  2:57756/140‑1 (MQ=255)                                                                                  gCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTagag                                                      >  2:908294/1‑140 (MQ=255)                                                                                     aGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATa                                                   >  1:524808/1‑140 (MQ=255)                                                                                      ggTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATaa                                                  >  1:67208/1‑140 (MQ=255)                                                                                             tcagtcagTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCa                                           <  2:1169996/140‑1 (MQ=255)                                                                                             tcagtcagTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCa                                           <  1:640544/140‑1 (MQ=255)                                                                                             tcagtcagTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGACAGGAGCCAATTTGCTGGTAGAGATAAAACGGCa                                           <  1:851641/140‑1 (MQ=255)                                                                                                 tcagtTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTaga                                                       >  2:776789/1‑124 (MQ=255)                                                                                                 tcagtTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTaga                                                       <  1:776789/124‑1 (MQ=255)                                                                                                    gtTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGTGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCg                                    <  1:1419592/140‑1 (MQ=255)                                                                                                    gtTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCg                                    <  1:125129/140‑1 (MQ=255)                                                                                                     ttttAACATTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGCGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCgg                                   >  1:389004/1‑140 (MQ=255)                                                                                                         aaCATTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTAGTAGAGATAAAACGGCAGGGTGCGGAAat                               >  2:461610/1‑140 (MQ=255)                                                                                                            aTTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAatcat                            <  1:93659/140‑1 (MQ=255)                                                                                                            aTTTCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAatcat                            <  1:1026836/140‑1 (MQ=255)                                                                                                               tCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGt                         >  2:839482/1‑140 (MQ=255)                                                                                                               tCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGt                         >  2:1391888/1‑140 (MQ=255)                                                                                                               tCGCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGt                         >  2:178050/1‑140 (MQ=255)                                                                                                                 gCCCCGGTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTa                       <  1:1246929/140‑1 (MQ=255)                                                                                                                       gTAGTTTTTCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGaac                 >  1:1016389/1‑140 (MQ=255)                                                                                                                              ttCAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGcca          <  2:639774/140‑1 (MQ=255)                                                                                                                                cAATCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGccacc        >  2:345124/1‑140 (MQ=255)                                                                                                                                  aTCACGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCcaccac      >  2:269059/1‑140 (MQ=255)                                                                                                                                     aCGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGcc   >  2:952183/1‑140 (MQ=255)                                                                                                                                      cGGTTAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCa  <  2:948575/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        CCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCA  >  NC_000913/72819‑73091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑