Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,442,740	(T)5→4	coding (977/2007 nt)	mnmC →	fused 5‑methylaminomethyl‑2‑thiouridine‑forming methyltransferase and FAD‑dependent demodification enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,442,736	0	T	.	100.0%	104.0 / NA	25	coding (973/2007 nt)	mnmC	fused 5‑methylaminomethyl‑2‑thiouridine‑forming methyltransferase and FAD‑dependent demodification enzyme
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (14/11);  total (14/11)

CTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAATTTGATCATGACTGGTGCGGCGTCACGCAGTTAGGCTGGGATGAGAAAAGCCAGCATAAAATCGCACAGATGTTGTCAA  >  NC_000913/2442610‑2442871                                                                                                                               |                                                                                                                                        cTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGC‑TTTTCTCTAATGCg                                                                                                                           <  1:309242/140‑1 (MQ=255)              gCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGC‑TTTTCTCTAATGCGTTTACTTTTGCtc                                                                                                              >  1:312024/1‑140 (MQ=255)               cTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGC‑TTTTCTCTAATGCGTTTACTTTTGCtcg                                                                                                             <  1:117981/140‑1 (MQ=255)                                aTTGTGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGC‑TTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAAt                                                                                            >  2:229656/1‑140 (MQ=255)                                aTTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGC‑TTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAAt                                                                                            >  2:331404/1‑140 (MQ=255)                                        gATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGC‑TTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGtt                                                                                    <  2:424093/140‑1 (MQ=255)                                            aGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGC‑TTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAAt                                                                                <  1:19617/140‑1 (MQ=255)                                            aGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGC‑TTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAAt                                                                                <  1:222176/140‑1 (MQ=255)                                                          ggTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGC‑TTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAATTTGATCATGACTgg                                                                  >  1:191299/1‑140 (MQ=255)                                                            tGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGC‑TTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAATTTGATCATGACTGGTg                                                                >  1:375761/1‑140 (MQ=255)                                                                tCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGC‑TTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAATTTGATCATGACTGGTgcggc                                                            >  2:225982/1‑140 (MQ=255)                                                                  cGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGC‑TTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAATTTGATCATGACTGGTGCGGCGt                                                          >  2:70713/1‑140 (MQ=255)                                                                    gCAATCGCCAGGGGGCGCTGTATCCGTTATTTAGCAAACACGATGAGGCGCTAAACCGC‑TTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAATTTGATCATGACTGGTGCGGCGTCa                                                        >  2:374056/1‑140 (MQ=255)                                                                    gCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGC‑TTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAATTTGATCATGACTGGTGCGGCGTCa                                                        <  1:331404/140‑1 (MQ=255)                                                                           ccAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGC‑TTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAATTTGATCATGACTGGTGCGGCGTCACGCAGtt                                                 <  2:312024/140‑1 (MQ=255)                                                                                             gTTATTAAGCAAACACGATGAGGCGCTAAACCGC‑TTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAATTTGATCATGACTGGTGCGGCGTCACGCAGTTAGGCTGGGATGAGAAAAg                               >  1:279892/1‑140 (MQ=255)                                                                                                    aGCAAACACGATGAGGCGCTAAACCGC‑TTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAATTTGATCATGACTGGTGCGGCGTCACGCAGTTAGGCTGGGATGAGAAAAGCCAGCAt                        >  1:403774/1‑140 (MQ=255)                                                                                                      cAAACACGATGAGGCGCTAAACCGC‑TTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAATTTGATCATGACTGGTGCGGCGTCACGCAGTTAGGCTGGGATGAGAAAAGCCAGCATaa                      >  1:386603/1‑140 (MQ=255)                                                                                                             gATGAGGCGCTAAACCGC‑TTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAATTTGATCATGACTGGTGCGGCGTCACGCAGTTAGGCTGGGATGAGAAAAGCCAGCATAAAATCGca               <  1:424833/140‑1 (MQ=255)                                                                                                                gAGGCGCTAAACCGC‑TTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAATTTGATCATGACTGGTGCGGCGTCACGCAGTTAGGCTGGGATGAGAAAAGCCAGCATAAAATCGCACAg            >  1:112856/1‑140 (MQ=255)                                                                                                                  ggCGCTAAACCGC‑TTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAATTTGATCATGACTGGTGCGGCGTCACGCAGTTAGGCTGGGATGAGAAAAGCCAGCATAAAATCGCACAGAt          >  1:264965/1‑140 (MQ=255)                                                                                                                   gcgcTAAACCGC‑TTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAATTTGATCATGACTGGTGCGGCGTCACGCAGTTAGGCTGGGATGAGAAAAGCCAGCATAAAATCGCACAGAtg         >  1:71808/1‑140 (MQ=255)                                                                                                                       tAAACCGC‑TTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAATTTGATCATGACTGGTGCGGCGTCACGCAGTTAGGCTGGGATGAGAAAAGCCAGCATAAAATCGCACAGAtgttgt     >  1:55996/1‑140 (MQ=255)                                                                                                                         aaCCGC‑TTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAATTTGATCATGACTGGTGCGGCGTCACGCAGTTAGGCTGGGATGAGAAAAGCCAGCATAAAATCGCACAGATGTTGTCa   <  2:18890/140‑1 (MQ=255)                                                                                                                         aaCCGC‑TTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAATTTGATCATGACTGGTGCGGCGTCACGCAGTTAGGCTGGGATGAGAAAAGCCAGCATAAAATCGCACAGATGTTGTCa   <  2:375761/140‑1 (MQ=255)                                                                                                                          aCCGC‑TTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAATTTGATCATGACTGGTGCGGCGTCACGCAGTTAGGCTGGGATGAGAAAAGCCAGCATAAAATCGCACAGATGTTGTCaa  <  1:225982/140‑1 (MQ=255)                                                                                                                               |                                                                                                                                        CTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAATTTGATCATGACTGGTGCGGCGTCACGCAGTTAGGCTGGGATGAGAAAAGCCAGCATAAAATCGCACAGATGTTGTCAA  >  NC_000913/2442610‑2442871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑