Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,432,016	T→C	intergenic (‑33/+75)	ytfE ← / ← ytfF	iron‑sulfur cluster repair protein YtfE/inner membrane protein YtfF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,432,016	0	T	C	100.0%	95.9 / NA	26	intergenic (‑33/+75)	ytfE/ytfF	iron‑sulfur cluster repair protein YtfE/inner membrane protein YtfF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/13);  total (13/13)

ACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGCATCTTTAAGGCAAAAGCTATAACCCTTACGTAGCAGAGGCTTATTTAACTCACTGCCAGCGTGTCAACTTTCTGAGACAGATTCAGTTAAAGGTTTTTCTGGTTTTGCTCTGACCGCG  >  NC_000913/4431885‑4432145                                                                                                                                    |                                                                                                                                  aCCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAAGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGCATTTTaaa                                                                                                                           <  2:397634/140‑1 (MQ=255)                 tCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGCATTTTAAATGCATCTTTAAGGCaa                                                                                                           <  2:95034/140‑1 (MQ=255)                  ccATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGCATTTTAAATGCATCTTTAAGGCaaa                                                                                                          <  2:348911/140‑1 (MQ=255)                     tatcatatTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGCATTTTAAATGCATCTTTAAGGCAAAAGc                                                                                                       >  1:252558/1‑140 (MQ=255)                     tatcatatTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGCATTTTAAATGCATCTTTAAGGCAAAAGc                                                                                                       >  2:157022/1‑140 (MQ=255)                        catatTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGCATTTTAAATGCATCTTTAAGGCAAAAGCtat                                                                                                    <  1:281804/140‑1 (MQ=255)                        catatTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGCATTTTAAATGCATCTTTAAGGCAAAAGCtat                                                                                                    <  2:205587/140‑1 (MQ=255)                                cGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGCATTTTAAATGCATCTTTAAGGCAAAAGCTATAACCCTTa                                                                                            >  2:407105/1‑140 (MQ=255)                                        agCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGCATTTTAAATGCATCTTTAAGGCAAAAGCTATAACCCTTACGTAGCag                                                                                    >  1:194215/1‑140 (MQ=255)                                             aaGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGCATTTTAAATGCATCTTTAAGGCAAAAGCTATAACCCTTACGTAGCAGAGGCt                                                                               >  2:382712/1‑140 (MQ=255)                                                  cgAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGCATTTTAAATGCATCTTTAAGGCAAAAGCTATAACCCTTACGTAGCAGAGGCTTAttt                                                                          >  2:310426/1‑140 (MQ=255)                                                                cgcCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGCATTTTAAATGCATCTTTAAGGCAAAAGCTATAACCCTTACGTAGCAGAGGCTTATTTAACTCACTGCCAGc                                                            >  1:405908/1‑140 (MQ=255)                                                                                 gTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGCATTTTAAATGCATCTTTAAGGCAAAAGCTATAACCCTTACGTAGCAGAGGCTTATTTAACTCACTGCCAGCGTGTCAACTTTCTgaga                                           <  1:157022/140‑1 (MQ=255)                                                                                  ttGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGCATTTTAAATGCATCTTTAAGGCAAAAGCTATAACCCTTACGTAGCAGAGGCTTATTTAACTCACTGCCAGCGTGTCAACTTTCTGAGAc                                          >  1:212377/1‑140 (MQ=255)                                                                                    ggTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGCATTTTAAATGCATCTTTAAGGCAAAAGCTATAACCCTTACGTAGCAGAGGCTTATTTAACTCACTGCCAGCGTGTCAACTTTCTGAGACAg                                        >  2:330611/1‑140 (MQ=255)                                                                                     gTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGCATTTTAAATGCATCTTTAAGGCAAAAGCTATAACCCTTACGTAGCAGAGGCTTATTTAACTCACTGCCAGCGTGTCAACTTTCTGAGACAGa                                       >  1:38083/1‑140 (MQ=255)                                                                                     gTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGCATTTTAAATGCATCTTTAAGGCAAAAGCTATAACCCTTACGTAGCAGAGGCTTATTTAACTCACTGCCAGCGTGTCAACTTTCTGAGACAGa                                       >  1:308989/1‑140 (MQ=255)                                                                                       cgcgATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGCATTTTAAATGCATCTTTAAGGCAAAAGCTATAACCCTTACGTAGCAGAGGCTTATTTAACTCACTGCCAGCGTGTCAACTTTCTGAGACAGAtt                                     <  1:407105/140‑1 (MQ=255)                                                                                       cgcgATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGCATTTTAAATGCATCTTTAAGGCAAAAGCTATAACCCTTACGTAGCAGAGGCTTATTTAACTCACTGCCAGCGTGTCAACTTTCTGAGACAGAtt                                     <  1:310426/140‑1 (MQ=255)                                                                                            tAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGCATTTTAAATGCATCTTTAAGGCAAAAGCTATAACCCTTACGTAGCAGAGGCTTATTTAACTCACTGCCAGCGTGTCAACTTTCTGAGACAGATTCAGtt                                <  1:382712/140‑1 (MQ=255)                                                                                            tAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGCATTTTAAATGCATCTTTAAGGCAAAAGCTATAACCCTTACGTAGCAGAGGCTTATTTAACTCACTGCCAGCGTGTCAACTTTCTGAGACAGATTCAGtt                                <  2:308989/140‑1 (MQ=255)                                                                                                       tGATACCTCATTCTTAATAAGATAAGATGCATTTTAAATGCATCTTTAAGGCAAAAGCTATAACCCTTACGTAGCAGAGGCTTATTTAACTCACTGCCAGCGTGTCAACTTTCTGAGACAGATTCAGTTAAAGGTTTTTc                     <  2:405908/140‑1 (MQ=255)                                                                                                        gATACCTCATTCTTAATAAGATAAGATGCATTTTAAATGCATCTTTAAGGCAAAAGCTATAACCCTTACGTAGCAGAGGCTTATTTAACTCACTGCCAGCGTGTCAACTTTCTGAGACAGATTCAGTTAAAGGTTTTTCt                    >  1:245546/1‑140 (MQ=255)                                                                                                          tACCTCATTCTTAATAAGATAAGATGCATTTTAAATGCATCTTTAAGGCAAAAGCTATAACCCTTACGTAGCAGAGGCTTATTTAACTCACTGCCAGCGTGTCAACTTTCTGAGACAGATTCAGTTAAAGGTTTTTCTgg                  >  2:126168/1‑140 (MQ=255)                                                                                                                          agataagatGCATTTTAAATGCATCTTTAAGGCAAAAGCTATAACCCTTACGTAGCAGAGGCTTATTTAACTCACTGCCAGCGTGTCAACTTTCTGAGACAGATTCAGTTAAAGGTTTTTCTGGTTTTGCTCTGACcgcg  <  2:194215/140‑1 (MQ=255)                                                                                                                          agataagatGCATTTTAAATGCATCTTTAAGGCAAAAGCTATAACCCTTACGTAGCAGAGGCTTATTTAACTCACTGCCAGCGTGTCAACTTTCTGAGACAGATTCAGTTAAAGGTTTTTCTGGTTTTGCTCTGACcgcg  <  1:126168/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                  ACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATGTATTTTAAATGCATCTTTAAGGCAAAAGCTATAACCCTTACGTAGCAGAGGCTTATTTAACTCACTGCCAGCGTGTCAACTTTCTGAGACAGATTCAGTTAAAGGTTTTTCTGGTTTTGCTCTGACCGCG  >  NC_000913/4431885‑4432145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑