Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,904,072	G→A	A60T (GCT→ACT)	manZ →	mannose‑specific PTS enzyme IID component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,904,072	0	G	A	100.0%	67.5 / NA	21	A60T (GCT→ACT)	manZ	mannose‑specific PTS enzyme IID component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/8);  total (13/8)

CTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGGAAGAACAGCGTGCTAATGGCGCAGAGATCGACGACGGTGCTATCAA  >  NC_000913/1903935‑1904208                                                                                                                                          |                                                                                                                                         ctcaAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAACt                                                                                                                                        >  2:424617/1‑140 (MQ=255)           tatTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAACTCGTAAACAAg                                                                                                                              >  2:123440/1‑140 (MQ=255)                  ggCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAACTCGTAAACAAGCTATTCg                                                                                                                       >  1:251737/1‑140 (MQ=255)                  ggCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAACTCGTAAACAAGCTATTCg                                                                                                                       <  2:199661/140‑1 (MQ=255)                                      ccTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAACTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTa                                                                                                   <  2:390016/140‑1 (MQ=255)                                      ccTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAACTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTa                                                                                                   <  2:418790/140‑1 (MQ=255)                                               gggTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAACTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGc                                                                                          >  2:240112/1‑140 (MQ=255)                                                    cATGGAACTTCGAACGTATGCAGGCACTGGGCTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAACTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTc                                                                                     >  1:56605/1‑140 (MQ=255)                                                         aaCTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAACTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGc                                                                                <  1:424617/140‑1 (MQ=255)                                                              cGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAACTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTgcgc                                                                           >  1:178789/1‑140 (MQ=255)                                                                               tGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAACTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAAcc                                                          >  2:382713/1‑140 (MQ=255)                                                                               tGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAACTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAAcc                                                          >  1:185406/1‑140 (MQ=255)                                                                                                 tGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAACTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGGAAGAACAg                                        <  1:240112/140‑1 (MQ=255)                                                                                                         gCAATTCGTCGCCTCTACCCTGAGAACAACGAAACTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGGAAGAACAGCGTGCTaa                                >  1:353882/1‑140 (MQ=255)                                                                                                         gCAATTCGTCGCCTCTACCCTGAGAACAACGAAACTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGGAAGAACAGCGTGCTaa                                >  2:335591/1‑140 (MQ=255)                                                                                                              tcgtcgCCTCTACCCTGAGAACAACGAAACTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGGAAGAACAGCGTGCTAATGgcg                           >  2:323911/1‑140 (MQ=255)                                                                                                                               agaACAACGAAACTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGGAAGAACAGCGTGCTAATGGCGCAGAGATCGACGACGGt          <  2:56164/140‑1 (MQ=255)                                                                                                                               agaACAACGAAACTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGGAAGAACAGCGTGCTAATGGCGCAGAGATCGACGACGGt          >  1:364398/1‑140 (MQ=255)                                                                                                                               agaACAACGAAACTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGGAAGAACAGCGTGCTAATGGCGCAGAGATCGACGACGGt          <  1:327553/140‑1 (MQ=255)                                                                                                                                    aacGAAACTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGGAAGAACAGCGTGCTAATGGCGCAGAGATCGACGACGGTGCTAt     >  2:208120/1‑140 (MQ=255)                                                                                                                                       gAAACTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGGAAGAACAGCGTGCTAATGGCGCAGAGATCGACGACGGTGCTATCaa  <  2:178789/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         CTCAAAGTGATATTCGTGGCGTCTTCCTGCGTTCTAACCTCTTCCAGGGTTCATGGAACTTCGAACGTATGCAGGCACTGGGTTTCTGCTTCTCTATGGTACCGGCAATTCGTCGCCTCTACCCTGAGAACAACGAAGCTCGTAAACAAGCTATTCGCCGTCACCTGGAGTTCTTTAACACCCAGCCGTTCGTGGCTGCGCCGATTCTCGGCGTAACCCTGGCGCTGGAAGAACAGCGTGCTAATGGCGCAGAGATCGACGACGGTGCTATCAA  >  NC_000913/1903935‑1904208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑