Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,071,379	C→T	T126T (ACG→ACA)	yihS ←	sulfoquinovose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,071,379	0	C	T	98.7%	258.3 / ‑4.2	77	T126T (ACG→ACA)	yihS	sulfoquinovose isomerase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (37/39);  total (38/39)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.94e-01

TGCCGCCGCGGTACTCTTCGGTTTTGCTGAAGGCTTCGTCCCAGGATTCCAGGCACATCTGCTCTTCTTCGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCCGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCTGTTTGGAGGCATCCACCACGCCCTCGTCATTCACGCAGGCATACCAGCCGCCATATTTTTTATCACGCAGTGCGCCGTTCA  >  NC_000913/4071243‑4071512                                                                                                                                         |                                                                                                                                      tGCCGCCGCGGTACTCTTCGGTTTTGCTGAAGGCTTCGTCCCAGGATTCCAGGCACATCTGCTCTTCTTCGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGtt                                                                                                                                    >  2:1036277/1‑140 (MQ=255) tGCCGCCGCGGTACTCTTCGGTTTTGCTGAAGGCTTCGTCCCAGGATTCCAGGCACATCTGCTCTTCTTCGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGtt                                                                                                                                    >  1:663534/1‑140 (MQ=255)              ctctTCGGTTTTGCTGAAGGCTTCGTCCCAGGATTCCAGGCACATCTGCTCTTCTTCGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTgg                                                                                                                       <  1:787813/140‑1 (MQ=255)              ctctTCGGTTTTGCTGAAGGCTTCGTCCCAGGATTCCAGGCACATCTGCTCTTCTTCGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTgg                                                                                                                       >  2:95291/1‑140 (MQ=255)              ctctTCGGTTTTGCTGAAGGCTTCGTCCCAGGATTCCAGGCACATCTGCTCTTCTTCGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTgg                                                                                                                       <  2:773073/140‑1 (MQ=255)                ctTCGGTTTTGCTGAAGGCTTCGTCCCAGGATTCCAGGCACATCTGCTCTTCTTCGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTggcg                                                                                                                     <  1:90749/140‑1 (MQ=255)                ctTCGGTTTTGCTGAAGGCTTCGTCCCAGGATTCCAGGCACATCTGCTCTTCTTCGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTggcg                                                                                                                     <  1:282185/140‑1 (MQ=255)                   cGGTTTTGCTGAAGGCTTCGTCCCAGGATTCCAGGCACATCTGCTCTTCTTCGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCa                                                                                                                  <  2:167119/140‑1 (MQ=255)                      ttttGCTGAAGGCTTCGTCCCAGGATTCCAGGCACATCTGCTCTTCTTCGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCAccc                                                                                                               >  1:937700/1‑140 (MQ=255)                      ttttGCTGAAGGCTTCGTCCCAGGATTCCAGGCACATCTGCTCTTCTTCGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCAccc                                                                                                               >  2:323412/1‑140 (MQ=255)                      ttttGCTGAAGGCTTCGTCCCAGGATTCCAGGCACATCTGCTCTTCTTCGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCAccc                                                                                                               >  1:947202/1‑140 (MQ=255)                        ttGCTGAAGGCTTCGTCCCAGGATTCCAGGCACATCTGCTCTTCTTCGCTCCAGAAATATTTCGCGATAATTTCAATTGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCcag                                                                                                             <  2:635781/140‑1 (MQ=255)                           cTGAAGGCTTCGTCCCAGGATTCCAGGCACATCTGCTCTTCTTCGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCcagcag                                                                                                          >  2:829815/1‑140 (MQ=255)                               aGGCTTCGTCCCAGGATTCCAGGCACATCTGCTCTTCTTCGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCa                                                                                                      >  1:641825/1‑140 (MQ=255)                                 gCTTCGTCCCAGGATTCCAGGCACATCTGCTCTTCTTCGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGTGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCaaa                                                                                                    <  2:755967/140‑1 (MQ=255)                                 gCTTCGTCCCAGGATTCCAGGCACATCTGCTCTTCTTCGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCaaa                                                                                                    <  2:732593/140‑1 (MQ=255)                                   ttCGTCCCAGGATTCCAGGCACATCTGCTCTTCTTCGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAaaga                                                                                                  <  1:1036277/140‑1 (MQ=255)                                   ttCGTCCCAGGATTCCAGGCACATCTGCTCTTCTTCGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAaaga                                                                                                  <  2:159430/140‑1 (MQ=255)                                    tCGTCCCAGGATTCCAGGCACATCTGCTCTTCTTCGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAaagaa                                                                                                 <  2:987743/140‑1 (MQ=255)                                    tCGTCCCAGGATTCCAGGCACATCTGCTCTTCTTCGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAaagaa                                                                                                 <  1:416902/140‑1 (MQ=255)                                     cGTCCCAGGATTCCAGGCACATCTGCTCTTCTTCGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAaagaag                                                                                                >  2:419655/1‑140 (MQ=255)                                     cGTCCCAGGATTCCAGGCACATCTGCTCTTCTTCGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAaagaag                                                                                                >  1:265002/1‑140 (MQ=255)                                     cGTCCCAGGATTCCAGGCACATCTGCTCTTCTTCGCTCCAGAAATATTCCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAaagaag                                                                                                >  2:458211/1‑140 (MQ=255)                                             gATTCCAGGCACATCTGCTCTTCTTCGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATa                                                                                        <  1:138761/140‑1 (MQ=255)                                                tCCAGGCACATCTGCTCTTCTTCGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGcc                                                                                     <  2:129472/140‑1 (MQ=255)                                                tCCAGGCACATCTGCTCTTCTTCGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGcc                                                                                     <  2:435048/140‑1 (MQ=255)                                                tCCAGGCACATCTGCTCTTCTTCGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGcc                                                                                     <  1:95291/140‑1 (MQ=255)                                                tCCAGGCACATCTGCTCTTCTTCGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGcc                                                                                     >  1:687681/1‑140 (MQ=255)                                                  cAGGCACATCTGCTCTTCTTCGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCt                                                                                   >  1:366423/1‑140 (MQ=255)                                                  cAGGCACATCTGCTCTTCTTCGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCt                                                                                   >  2:878474/1‑140 (MQ=255)                                                  cAGGCACATCTGCTCTTCTTCGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCt                                                                                   <  1:698215/140‑1 (MQ=255)                                                  cAGGCACATCTGCTCTTCTTCGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCt                                                                                   >  1:938043/1‑140 (MQ=255)                                                       acaTCTGCTCTTCTTCGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCTGTTTg                                                                              >  1:43602/1‑140 (MQ=255)                                                       acaTCTGCTCTTCTTCGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCTGTTTg                                                                              >  2:326783/1‑140 (MQ=255)                                                       acaTCTGCTCTTCTTCGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCTGTTTg                                                                              >  1:663670/1‑140 (MQ=255)                                                       acaTCTGCTCTTCTTCGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCTGTTTg                                                                              <  1:11328/140‑1 (MQ=255)                                                         aTCTGCTCTTCTTCGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCTGTTTGGa                                                                            >  2:748924/1‑140 (MQ=255)                                                           cTGCTCTTCTTCGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCTGTTTGGAgg                                                                          >  2:588846/1‑140 (MQ=255)                                                             gCTCTTCTTCGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCTGTTTGGAGGCa                                                                        <  1:269908/140‑1 (MQ=255)                                                             gCTCTTCTTCGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCTGTTTGGAGGCa                                                                        <  1:323412/140‑1 (MQ=255)                                                             gCTCTTCTTCGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCTGTTTGGAGGCa                                                                        <  2:152463/140‑1 (MQ=255)                                                              ctctTCTTCGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCTGTTTGGAGGCAt                                                                       >  1:790134/1‑140 (MQ=255)                                                                   cttcGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCTGTTTGGAGGCATccacc                                                                  >  2:39646/1‑140 (MQ=255)                                                                   cttcGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCTGTTTGGAGGCATccacc                                                                  >  2:462061/1‑140 (MQ=255)                                                                   cttcGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCTGTTCGGAGGCATccacc                                                                  >  1:617781/1‑140 (MQ=255)                                                                    ttcGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCTGTTTGGAGGCATccacca                                                                 <  2:584145/140‑1 (MQ=255)                                                                      cGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCTGTTTGGAGGCATCCACCACg                                                               >  1:537197/1‑140 (MQ=255)                                                                      cGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCTGTTTGGAGGCATCCACCACg                                                               >  2:878411/1‑140 (MQ=255)                                                                        cTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCTGTTTGGAGGCATCCACCACGcc                                                             >  2:29391/1‑140 (MQ=255)                                                                                 tatTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCTGTTTGGAGGCATCCACCACGCCCTCGTCAtt                                                    <  1:607986/140‑1 (MQ=255)                                                                                    ttCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCTGTTTGGAGGCATCCACCACGGCCTCGTCATTCAc                                                 <  1:407572/140‑1 (MQ=255)                                                                                    ttCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCTGTTTGGAGGCATCCACCACGCCCTCGTCATTCAc                                                 <  2:704978/140‑1 (MQ=255)                                                                                    ttCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCTGTTTGGAGGCATCCACCACGCCCTCGTCATTCAc                                                 <  1:313692/140‑1 (MQ=255)                                                                                    ttCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCTGTTTGGAGGCATCCACCACGCCCTCGTCATTCAc                                                 <  2:687681/140‑1 (MQ=255)                                                                                    ttCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCTGTTTGGAGGCATCCACCACGCCCTCGTCATTCAc                                                 <  1:1008799/140‑1 (MQ=255)                                                                                    ttCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCTGTTTGGAGGCATCCACCACGCCCTCGTCATTCAc                                                 <  2:530202/140‑1 (MQ=255)                                                                                     tctcGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCTGTTTGGAGGCATCCACCACGCCCTCGTCATTCACg                                                >  2:826575/1‑140 (MQ=255)                                                                                     tctcGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCTGTTTGGAGGCATCCACCACGCCCTCGTCATTCACg                                                <  1:197208/140‑1 (MQ=255)                                                                                     tctcGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCTGTTTGGAGGCATCCACCACGCCCTCGTCATTCACg                                                >  1:852813/1‑140 (MQ=255)                                                                                                    tGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCTGTTTGGAGGCATCCACCACGCCCTCGTCATTCACGCAGGCATACCAgccg                                 <  1:878411/140‑1 (MQ=255)                                                                                                    tGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCTGTTTGGAGGCATCCACCACGCCCTCGTCATTCACGCAGGCATACCAgccg                                 <  1:462061/140‑1 (MQ=255)                                                                                                      gtgtAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCTGTTTGGAGGCATCCACCACGCCCTCGTCATTCACGCAGGCATACCAgccgcc                               <  1:326783/140‑1 (MQ=255)                                                                                                      gtgtAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCTGTTTGGAGGCATCCACCACGCCCTCGTCATTCACGCAGGCATACCAgccacc                               <  2:869274/140‑1 (MQ=255)                                                                                                       tgtAATCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCTGTTTGGAGGCATCCACCACGCCCTCGTCATTCACGCAGGCATACCAGCCGCCa                              >  2:966251/1‑140 (MQ=255)                                                                                                            tCGAGCAGCTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCTGTTTGGAGGCATCCACCACGCCCTCGTCATTCACGCAGGCATACCAGCCGCCATAttt                         >  1:1027853/1‑140 (MQ=255)                                                                                                                gcagcTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCTGTTTGGAGGCATCCACCACGCCCTCGTCATTCACGCAGGCATACCAGCCGCCATATTTTTTa                     >  1:914630/1‑140 (MQ=255)                                                                                                                gcagcTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCTGTTTGGAGGCATCCACCACGCCCTCGTCATTCACGCAGGCATACCAGCCGCCATATTTTTTa                     >  1:309590/1‑140 (MQ=255)                                                                                                                gcagcTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCTGTTTGGAGGCATCCACCACGCCCTCGTCATTCACGCAGGCATACCAGCCGCCATATTTTTTa                     >  1:95085/1‑140 (MQ=255)                                                                                                                 cagcTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCTGTTTGGAGGCATCCACCACGCCCTCGTCATTCACGCAGGCATACCAGCCGCCATATTTTTTAt                    >  2:643727/1‑140 (MQ=255)                                                                                                                 cagcTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCTGTTTGGAGGCATCCACCACGCCCTCGTCATTCACGCAGGCATACCAGCCGCCATATTTTTTAt                    >  2:429625/1‑140 (MQ=255)                                                                                                                  agcTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCTGTTTGGAGGCATCCACCACGCCCTCGTCATTCACGCAGGCATACCAGCCGCCATATTTTTTATc                   <  2:710220/140‑1 (MQ=255)                                                                                                                  agcTTGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCTGTTTGGAGGCATCCACCACGCCCTCGTCATTCACGCAGGCATACCAGCCGCCATATTTTTTATc                   <  2:744117/140‑1 (MQ=255)                                                                                                                      tGCGCGCTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCTGTTTGGAGGCATCCACCACGCCCTCGTCATTCACGCAGGCATACCAGCCGCCATATTTTTTATCACGc               >  2:786856/1‑140 (MQ=255)                                                                                                                       gcgcgcTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCTGTTTGGAGGCATCCACCACGCCCTCGTCATTCACGCAGGCATACCAGCCGCCATATTTTTTATCACGCa              <  1:748924/140‑1 (MQ=255)                                                                                                                            cTTCCGGGTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCTGTTTGGAGGCATCCACCACGCCCTCGTCATTCACGCAGGCATACCAGCCGCCATATTTTTTATCACGCAGTgcg         <  1:829815/140‑1 (MQ=255)                                                                                                                              tCCGGGTGACCCGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGAGTTGATAGCCCTGTTTGGAGGCATCCACCACGCCCTCGTCATTCACGCAGGCATACCAGCCGCCATATTTTTTATCACGCAGTGCGcc       >  2:690284/1‑140 (MQ=255)                                                                                                                                   gTGACCTGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCTGTTTGGAGGCATCCACCACGCCCTCGTCATTCACGCAGGCATACCAGCCGCCATATTTTTTATCACGCAGTGCGCCGTTCa  <  2:947202/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      TGCCGCCGCGGTACTCTTCGGTTTTGCTGAAGGCTTCGTCCCAGGATTCCAGGCACATCTGCTCTTCTTCGCTCCAGAAATATTTCTCGATAATTTCAATGGTGTAATCGAGCAGCTTGCGCGCTTCCGGGTGACCCGTTGTGACGGCGCTGGCGGCACCCAGCAGAGCAAAGAAGTGTTGATAGCCCTGTTTGGAGGCATCCACCACGCCCTCGTCATTCACGCAGGCATACCAGCCGCCATATTTTTTATCACGCAGTGCGCCGTTCA  >  NC_000913/4071243‑4071512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑