Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,128,623	Δ1 bp	intergenic (‑228/‑49)	metJ ← / → metB	DNA‑binding transcriptional repressor MetJ/O‑succinylhomoserine(thiol)‑lyase/O‑ succinylhomoserine lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,128,621	0	T	.	100.0%	328.0 / NA	77	intergenic (‑226/‑51)	metJ/metB	DNA‑binding transcriptional repressor MetJ/O‑succinylhomoserine(thiol)‑lyase/O‑ succinylhomoserine lyase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (47/30);  total (47/30)

ACAGGCGTTACCGTGACATCGTGTAATGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATGTTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCA‑‑GTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCAT  >  NC_000913/4128483‑4128755                                                                                                                                           |                                                                                                                                         aCAGGCGTTACCGTGACATCGTGTAATGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATGtt                                                                                                                                         >  1:140644/1‑140 (MQ=255)          aCCGTGACATCGTGTAATGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTg                                                                                                                               >  1:898050/1‑140 (MQ=255)          aCCGTGACATCGTGTAATGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTg                                                                                                                               >  1:55118/1‑140 (MQ=255)              tGACATCGTGTAATGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTGGGATTTGCTCAATCTATACGCTAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTg                                                                                                                           <  2:162119/140‑1 (MQ=255)               gACATCGTGTAATGCACCTGTCGGCGTGATAATGCATATAATTTTAATGGCTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGa                                                                                                                          >  1:813606/1‑140 (MQ=255)               gACATCGTGTAATGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGa                                                                                                                          >  2:583127/1‑140 (MQ=255)               gACATCGTGTAATGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGa                                                                                                                          >  1:498107/1‑140 (MQ=255)                aCATCGTGTAATGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGAc                                                                                                                         <  2:1073573/140‑1 (MQ=255)                   tCGTGTAATGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACAtt                                                                                                                      >  2:196065/1‑140 (MQ=255)                      tgtAATGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCa                                                                                                                   <  2:128377/140‑1 (MQ=255)                            gCACCTGTCGGCGTTATAATGCATATAATTTTAACGGCTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACa                                                                                                             <  1:677895/140‑1 (MQ=255)                            gCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACa                                                                                                             <  2:434925/140‑1 (MQ=255)                            gCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACa                                                                                                             <  2:540410/140‑1 (MQ=255)                             cACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACaa                                                                                                            >  1:687993/1‑140 (MQ=255)                             cACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTG‑CGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACaaa                                                                                                           >  2:229236/1‑140 (MQ=255)                                cTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGc                                                                                                         >  2:940751/1‑140 (MQ=255)                                  gTCGGCGTGATAATGCATATAATTTTAACGGCTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGccc                                                                                                       >  1:60342/1‑140 (MQ=255)                                      gCGTGATAATGCATATAATTTTAACGGCTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAggg                                                                                                   <  1:583127/140‑1 (MQ=255)                                      gCGTGATAATGCATATAATTTTAACGGCTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAggg                                                                                                   >  2:749764/1‑140 (MQ=255)                                      gCGTGATAATGCATATAATTTTAACGGCTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAggg                                                                                                   >  1:24964/1‑140 (MQ=255)                                       cGTGATAATGCATATAATTTTAACGGCTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGa                                                                                                  <  2:171767/140‑1 (MQ=255)                                       cGTGATAATGCATATAATTTTAACGGCTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGa                                                                                                  >  2:382494/1‑140 (MQ=255)                                                   tataATTTTAACGGCTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACAt                                                                                      >  1:598788/1‑140 (MQ=255)                                                     taATTTTAACGGCTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGa                                                                                    >  1:352813/1‑140 (MQ=255)                                                     taATTTTAACGGCTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGa                                                                                    >  1:992857/1‑140 (MQ=255)                                                      aaTTTTAACGGCTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGAc                                                                                   >  1:733978/1‑140 (MQ=255)                                                        ttttAACGGCTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAAGGAACTTCATCACATGAcgc                                                                                 >  2:1064901/1‑140 (MQ=255)                                                           tAACGGCTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTa                                                                              >  1:1075229/1‑140 (MQ=255)                                                            aaCGGCTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCTTTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTaa                                                                             >  1:507964/1‑140 (MQ=255)                                                            aaCGGCTATTTGGGATTTGCTCAATCTATACGCAAAGAAGGTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTaa                                                                             >  2:510851/1‑140 (MQ=255)                                                               ggCTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTG‑CGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAg                                                                         <  1:229236/140‑1 (MQ=255)                                                                gCTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAg                                                                         >  2:812740/1‑140 (MQ=255)                                                                 cTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAgg                                                                        >  2:13356/1‑140 (MQ=255)                                                                  tATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTTACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGc                                                                       <  2:217494/140‑1 (MQ=255)                                                                  tATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGc                                                                       <  1:1044040/140‑1 (MQ=255)                                                                       gggATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGccacca                                                                  <  2:905207/140‑1 (MQ=255)                                                                         gATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATc                                                                >  2:695468/1‑140 (MQ=255)                                                                          aTTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCg                                                               <  1:635269/140‑1 (MQ=255)                                                                              gCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCA‑‑Gt                                                         >  2:114780/1‑140 (MQ=255)                                                                               cTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGccac                                                                    <  1:397577/130‑1 (MQ=255)                                                                               cTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGccac                                                                    >  2:397577/1‑130 (MQ=255)                                                                                        taCGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCA‑‑GTGCGTAGCggg                                               >  2:455031/1‑140 (MQ=255)                                                                                         aCGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCA‑‑GTGCGTAGCGGGt                                              >  1:320586/1‑140 (MQ=255)                                                                                         aCGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCA‑‑GTGCGTAGCGGGt                                              >  1:249185/1‑140 (MQ=255)                                                                                            cAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCAGTGTGCGTAGCGGGtt                                             >  1:112155/1‑140 (MQ=255)                                                                                             aaaGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCA‑‑GTGCGTAGCGGGTTaaa                                          <  1:961416/140‑1 (MQ=255)                                                                                              aagaagTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACGGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCA‑‑GTGCGTAGCGGGTTAAAt                                         >  1:606763/1‑140 (MQ=255)                                                                                              aagaagTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCA‑‑GTGCGTAGCGGGTTAAAt                                         >  1:958222/1‑140 (MQ=255)                                                                                              aagaagTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCA‑‑GTGCGTAGCGGGTTAAAt                                         >  1:325093/1‑140 (MQ=255)                                                                                              aagaagTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCA‑‑GTGCGTAGCGGGTTAAAt                                         >  2:1063913/1‑140 (MQ=255)                                                                                              aagaagTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCA‑‑GTGCGTAGCGGGTTAAAt                                         >  2:960280/1‑140 (MQ=255)                                                                                              aagaagTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCA‑‑GTGCGTAGCGGGTTAAAt                                         >  1:208338/1‑140 (MQ=255)                                                                                                  agTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCA‑‑GTGCGTAGCGGGTTAAATgacg                                     <  2:352813/140‑1 (MQ=255)                                                                                                  agTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCA‑‑GTGCGTAGCGGGTTAAATgacg                                     <  1:382494/140‑1 (MQ=255)                                                                                                     ttAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCA‑‑GTGCGTAGCGGGTTAAATGacgacg                                  <  2:806658/140‑1 (MQ=255)                                                                                                      tAGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCA‑‑GTGCGTAGCGGGTTAAATGAcgacga                                 >  1:217482/1‑140 (MQ=255)                                                                                                       aGATGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCA‑‑GTGCGTAGCGGGTTAAATGACGACGaa                                <  1:910279/140‑1 (MQ=255)                                                                                                          tGTCCAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCA‑‑GTGCGTAGCGGGTTAAATGACGACGAACAg                             <  1:13356/140‑1 (MQ=255)                                                                                                              cAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCA‑‑GTGCGTAGCGGGTTAAATGACGACGAACAGTATg                         >  1:229365/1‑140 (MQ=255)                                                                                                              cAGATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCA‑‑GTGCGTAGCGGGTTAAATGACGACGAACAGTATg                         >  1:726320/1‑140 (MQ=255)                                                                                                                gATGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCA‑‑GTGCGTAGCGGGTTAAATGACGACGAACAGTATGGt                       <  2:154089/140‑1 (MQ=255)                                                                                                                 aTGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCA‑‑GTGCGTAGCGGGTTAAATGACGACGAACAGTATGGtt                      >  1:199939/1‑140 (MQ=255)                                                                                                                  tGTATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCA‑‑GTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTg                     <  2:687993/140‑1 (MQ=255)                                                                                                                    tATTGACGTCCTTTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCA‑‑GTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCg                   <  2:507964/140‑1 (MQ=255)                                                                                                                    tATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCA‑‑GTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCg                   <  1:940751/140‑1 (MQ=255)                                                                                                                    tATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCA‑‑GTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCg                   <  2:834762/140‑1 (MQ=255)                                                                                                                    tATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCA‑‑GTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCg                   <  2:249185/140‑1 (MQ=255)                                                                                                                    tATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCA‑‑GTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCg                   <  1:832874/140‑1 (MQ=255)                                                                                                                    tATTGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCA‑‑GTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCg                   >  1:381655/1‑140 (MQ=255)                                                                                                                      ttGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCA‑‑GTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGtt                 >  1:465463/1‑140 (MQ=255)                                                                                                                      ttGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCA‑‑GTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGtt                 >  2:869664/1‑140 (MQ=255)                                                                                                                      ttGACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCA‑‑GTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGtt                 >  1:184206/1‑140 (MQ=255)                                                                                                                        gACGTCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCA‑‑GTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGt               >  1:441069/1‑140 (MQ=255)                                                                                                                            tCCATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCA‑‑GTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCa           <  2:992857/140‑1 (MQ=255)                                                                                                                             ccATTAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCA‑‑GTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCAc          >  2:1015443/1‑140 (MQ=255)                                                                                                                                ttAACACAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCA‑‑GTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGa       >  2:379615/1‑140 (MQ=255)                                                                                                                                   acacAATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCA‑‑GTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATcc    <  1:510851/140‑1 (MQ=255)                                                                                                                                     acaATG‑TTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCCCCATCGCA‑‑GTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCAt  <  1:844158/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                         ACAGGCGTTACCGTGACATCGTGTAATGCACCTGTCGGCGTGATAATGCATATAATTTTAACGGCTATTTGGGATTTGCTCAATCTATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCATTAACACAATGTTTACTCTGGTGCCTGACATTTCACCGACAAAGCCCAGGGAACTTCATCACATGACGCGTAAACAGGCCACCATCGCA‑‑GTGCGTAGCGGGTTAAATGACGACGAACAGTATGGTTGCGTTGTCCCACCGATCCAT  >  NC_000913/4128483‑4128755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑