Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	634,013	C→T	A143V (GCA→GTA)	ybdL →	methionine‑oxo‑acid transaminase, PLP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	634,013	0	C	T	100.0%	186.4 / NA	57	A143V (GCA→GTA)	ybdL	methionine‑oxo‑acid transaminase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/26);  total (31/26)

GGCAGGGGCGACGGAAGCGTTATACGCGGCGATTACCGCACTGGTGCGCAATGGCGATGAAGTGATTTGTTTTGATCCCAGCTATGACAGTTACGCCCCCGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGCCGCATTATTAAGCGAGCGCACCAGACTGGTGATCCTCAACACTCCGCATAACCCCAGTGCAACTGTCTGGCAGCAGGCTGATTTCGCCGCTT  >  NC_000913/633876‑634147                                                                                                                                          |                                                                                                                                       ggCAGGGGCGACGGAAGCGTTATACGCGGCGATTACCGCACTGGTGCGCAATGGCGATGAAGTGATTTGTTTTGATCCCAGCTATGACAGTTACGCCCCCGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTAc                                                                                                                                      <  2:578046/140‑1 (MQ=255)    aGGGGCGACGGAAGCGTTATACGCGGCGATTACCGCACTGGTGCGCAATGGCGATGAAGTGATTTGTTTTGATCCCAGCTATGACAGTTACGCCCCCGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTACTGc                                                                                                                                   >  2:1038798/1‑140 (MQ=255)    aGGGGCGACGGAAGCGTTATACGCGGCGATTACCGCACTGGTGCGCAATGGCGATGAAGTGATTTGTTTTGATCCCAGCTATGACAGTTACGCCCCCGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTACTGc                                                                                                                                   >  1:645048/1‑140 (MQ=255)     ggggCGACGGAAGCGTTATACGCGGCGATTACCGCACTGGTGCGCAATGGCGATGAAGTGATTTGTTTTGATCCCAGCTATGACAGTTACGCCCCCGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTACTGCa                                                                                                                                  >  1:244254/1‑140 (MQ=255)      gggCGACGGAAGCGTTATACGCGGCGATTACCGCACTGGTGCGCAATGGCGATGAAGTGATTTGTTTTGATCCCAGCTATGACAGTTACG‑CCCCGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTACTGCAac                                                                                                                                >  1:425146/1‑140 (MQ=255)         cGACGGAAGCGTTATACGCGGCGATTACCGCACTGGTGCGCAATGGCGATGAAGTGATTTGTTTTGATCCCAGCTATGACAGTTACGCCCCCGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTACTGCAacca                                                                                                                              <  2:499500/140‑1 (MQ=255)                aGCGTTATACGCGGCGATTACCGCACTGGTGCGCAATGGCGATGAAGTGATTTGTTTTGATCCCAGCTATGACAGTTACGCCCCCGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTACTGCAACCACCGCAtt                                                                                                                       >  2:770575/1‑140 (MQ=255)                aGCGTTATACGCGGCGATTACCGCACTGGTGCGCAATGGCGATGAAGTGATTTGTTTTGATCCCAGCTATGACAGTTACGCCCCCGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTACTGCAACCACCGCAtt                                                                                                                       >  1:374034/1‑140 (MQ=255)                      ataCGCGGCGATTACCGCACTGGTGCGCAATGGCGATGAAGTGATTTGTTTTGATCCCAGCTATGACAGTTACGCCCCCGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTACTGCAACCACCGCATTTTcgcg                                                                                                                 <  1:476077/140‑1 (MQ=255)                            ggcgATTACCGCACTGGTGCGCAATGGCGATGAAGTGATTTGTTTTGATCCCAGCTATGACAGTTACGCCCCCGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTACTGCAACCACCGCATTTTCGCGTTGACt                                                                                                           >  1:1089520/1‑140 (MQ=255)                                          tGGTGCGCAATGGCGATGAAGTGATTTGTTTTGATCCCAGCTATGACAGTTACGCCCCCGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGcc                                                                                             <  2:244254/140‑1 (MQ=255)                                          tGGTGCGCAATGGCGATGAAGTGATTTGTTTTGATCCCAGCTATGACAGTTACGCCCCCGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGcc                                                                                             <  2:507397/140‑1 (MQ=255)                                          tGGTGCGCAATGGCGATGAAGTGATTTGTTTTGATCCCAGCTATGACAGTTACGCCCCCGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGcc                                                                                             <  2:509365/140‑1 (MQ=255)                                            gTGCGCAATGGCGATGAAGTGATTTGTTTTGATCCCAGCTATGACAGTTACGCCCCCGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGCCGc                                                                                           <  1:1158316/140‑1 (MQ=255)                                            gTGCGCAATGGCGATGAAGTGATTTGTTTTGATCCCAGCTATGACAGTTACGCCCCCGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGCCGc                                                                                           >  1:1045498/1‑140 (MQ=255)                                                 cAATGGCGATGAAGTGATTTGTTTTGATCCCAGCTATGACAGTTACGCCCCCGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGCCGCattat                                                                                      <  1:631901/140‑1 (MQ=255)                                                   aTGGCGATGAAGTGATTTGTTTTGATCCCAGCTATGACAGTTACG‑CCCCGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGCCGCATTATTaa                                                                                   <  2:425146/140‑1 (MQ=255)                                                         aTGAAGTGATTTGTTTTGATCCCAGCTATGACAGTTACGCCCCCGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGCCGCATTATTAagcgag                                                                              <  2:529799/140‑1 (MQ=255)                                                           gAAGTGATTTGTTTTGATCCCAGCTATGACAGTTACGCCCCCGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGCCGCATTATTAagcgagcg                                                                            <  2:165418/140‑1 (MQ=255)                                                                gATTTGTTTTGATCCCAGCTATGACAGTTACGCCCCCGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGCCGCATTATTAAGCGAGCGCACCa                                                                       <  2:374034/140‑1 (MQ=255)                                                                gATTTGTTTTGATCCCAGCTATGACAGTTACGCCCCCGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGCCGCATTATTAAGCGAGCGCACCa                                                                       >  1:910749/1‑140 (MQ=255)                                                                gATTTGTTTTGATCCCAGCTATGACAGTTACGCCCCCGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGCCGCATTATTAAGCGAGCGCACCa                                                                       >  1:119264/1‑140 (MQ=255)                                                                    tGTTTTGATCCCAGCTATGACAGTTACGCCCCCGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGCCGCATTATTAAGCGAGCGCACCAGACt                                                                   >  1:996236/1‑140 (MQ=255)                                                                        ttGATCCCAGCTATGACAGTTACGCCCCCGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGCCGCATTATTAAGCGAGCGCACCAGACTGGTg                                                               <  2:1089520/140‑1 (MQ=255)                                                                        ttGATCCCAGCTATGACAGTTACGCCCCCGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGCCGCATTATTAAGCGAGCGCACCAGACTGGTg                                                               <  2:1221142/140‑1 (MQ=255)                                                                        ttGATCCCAGCTATGACAGTTACGCCCCCGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGCCGCATTATTAAGCGAGCGCACCAGACTGGTg                                                               <  2:1049757/140‑1 (MQ=255)                                                                           aTCCCAGCTATGACAGTTACGCCCCCGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGCCGCATTATTAAGCGAGCGCACCAGACTGGTGATc                                                            <  2:476259/140‑1 (MQ=255)                                                                                aGCTATGACAGTTACGCCCCCGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTACTGCAACCACCGCATTTTCGCGTTGACTGGCATGAATTTGCCGCATTATTAAGCGAGCGCACCAGACTTGTTATCCTCaa                                                       >  2:58561/1‑140 (MQ=255)                                                                                aGCTATGACAGTTACGCCCCCGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGCCGCATTATTAAGCGAGCGCACCAGACTGGTGATCCTCaa                                                       <  1:701623/140‑1 (MQ=255)                                                                                aGCTATGACAGTTACGCCCCCGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGCCGCATTATTAAGCGAGCGCACCAGACTGGTGATCCTCaa                                                       <  2:1069891/140‑1 (MQ=255)                                                                                aGCTATGACAGTTACGCCCCCGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGCCGCATTATTAAGCGAGCGCACCAGACTGGTGATCCTCaa                                                       >  2:939417/1‑140 (MQ=255)                                                                                   tATGACAGTTACGCCCCCGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGCCGCATTATTAAGCGAGCGCACCAGACTGGTGATCCTCAacac                                                    <  2:910749/140‑1 (MQ=255)                                                                                   tATGACAGTTACGCCCCCGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATCTGCCGCATTATTAAGCGAGCGCACCAGACTGGTGATCCTCAacac                                                    <  2:119264/140‑1 (MQ=255)                                                                                     tGACAGTTACGCCCCCGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGCCGCATTATTAAGCGAGCGCACCAGACTGGTGATCCTCAACACTc                                                  <  2:1174739/140‑1 (MQ=255)                                                                                     tGACAGTTACGCCCCCGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGCCGCATTATTAAGCGAGCGCACCAGACTGGTGATCCTCAACACTc                                                  <  1:770575/140‑1 (MQ=255)                                                                                       aCAGTTACGCCCCCGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGCCGCATTATTAAGCGAGCGCACCAGACTGGTGATCCTCAACACTCCg                                                >  1:172523/1‑140 (MQ=255)                                                                                        cAGTTACGCCCCCGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGCCGCATTATTAAGCGAGCGCACCAGACTGGTGATCCTCAACACTCCGc                                               >  2:873296/1‑140 (MQ=255)                                                                                          gTTACGCCCCCGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGCCGCATTATTAAGCGAGCGCACCAGACTGGTGATCCTCAACACTCCGCAt                                             >  2:934440/1‑140 (MQ=255)                                                                                            tACGCCCCCGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGCCGCATTATTAAGCGAGCGCACCAGACTGGTGATCCTCAACACTCCGCATaa                                           >  2:99589/1‑140 (MQ=255)                                                                                             aCGCCCCCGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGCCGCATTATTAAGCGAGCGCACCAGACTGGTGATCCTCAACACTCCGCATAAc                                          >  1:362928/1‑140 (MQ=255)                                                                                             aCGCCCCCGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGCCGCATTATTAAGCGAGCGCACCAGACTGGTGATCCTCAACACTCCGCATAAc                                          <  2:1136170/140‑1 (MQ=255)                                                                                             aCGCCCCCGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGCCGCATTATTAAGCGAGCGCACCAGACTGGTGATCCTCAACACTCCGCATAAc                                          >  2:370767/1‑140 (MQ=255)                                                                                             aCGCCCCCGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGCCGCATTATTAAGCGAGCGCACCAGACTGGTGATCCTCAACACTCCGCATAAc                                          >  2:802624/1‑140 (MQ=255)                                                                                               gCCCCCGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGCCGCATTATTAAGCGAGCGCACCAGACTGGTGATCCTCAACACTCCGCATAAccc                                        >  1:966353/1‑140 (MQ=255)                                                                                                cccccGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGCCGCATTATTAAGCGAGCGCACCAGACTGGTGATCCTCAACACTCCGCATAAcccc                                       <  1:673711/140‑1 (MQ=255)                                                                                                 ccccGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGCCGCATTATTAAGCGAGCGCACCAGACTGGTGATCCTCAACACTCCGCATAACCCCa                                      <  1:638926/140‑1 (MQ=255)                                                                                                    cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGCCGCATTATTAAGCGAGCGCACCAGACTGGTGATCCTCAACACTCCGCATAACCCCAGTg                                   >  2:497811/1‑140 (MQ=255)                                                                                                       cATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGTACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGCCGCATTATTAAGCGAGCGCACCAGACTGGTGATCCTCAACACTCCGCATAACCCCAGTGCaa                                <  1:1087842/140‑1 (MQ=255)                                                                                                                    gggggAATAGTGAAGCGTATGGTACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGCCGCATTATTAAGCGAGCGCACCAGACTGGTGATCCTCAACACTCCGCATAACCCCAGTGCAACTGTCTGgcagca                   >  1:802796/1‑140 (MQ=255)                                                                                                                           tAGTGAAGCGTATGGTACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGCCGCATTATTAAGCGAGCGCACCAGACTGGTGATCCTCAACACTCCGCATAACCCCAGTGCAACTGTCTGGCAGCAGGCTGAt            >  1:925960/1‑140 (MQ=255)                                                                                                                           tAGTGAAGCGTATGGTACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGCCGCATTATTAAGCGAGCGCACCAGACTGGTGATCCTCAACACTCCGCATAACCCCAGTGCAACTGTCTGGCAGCAGGCTGAt            >  1:765744/1‑140 (MQ=255)                                                                                                                           tAGTGAAGCGTATGGTACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGCCGCATTATTAAGCGAGCGCACCAGACTGGTGATCCTCAACACTCCGCATAACCCCAGTGCAACTGTCTGGCAGCAGGCTGAt            >  2:90998/1‑140 (MQ=255)                                                                                                                            aGTGAAGCGTATGGTACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGCCGCATTATTAAGCGAGCGCACCAGACTGGTGATCCTCAACACTCCGCATAACCCCAGTGCAACTGTCTGGCAGCAGGCTGAtt           >  2:1031120/1‑140 (MQ=255)                                                                                                                                aaGCGTATGGTACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGCCGCATTATTAAGCGAGCGCACCAGACTGGTGATCCTCAACACTCCGCATAACCCCAGTGCAACTGTCTGGCAGCAGGCTGATTTcgc       <  1:873296/140‑1 (MQ=255)                                                                                                                                aaGCGTATGGTACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGCCGCATTATTAAGCGAGCGCACCAGACTGGTGATCCTCAACACTCCGCATAACCCCAGTGCAACTGTCTGGCAGCAGGCTGATTTcgc       >  1:85660/1‑140 (MQ=255)                                                                                                                                   cGTATGGTTCTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGCCGCATTATTAAGCGAGCGCACCAGACTGGTGATCCTCAACACTCCGCATAACCCCAGTGCAACTGTCTGGCAGCAGGCTGATTTcgccgc    >  2:138170/1‑140 (MQ=255)                                                                                                                                   cGTATGGTACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGCCGCATTATTAAGCGAGCGCACCAGACTGGTGATCCTCAACACTCCGCATAACCCCAGTGCAACTGTCTGGCAGCAGGCTGATTTcgccgc    >  2:478987/1‑140 (MQ=255)                                                                                                                                     tATGGTACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGCCGCATTATTAAGCGAGCGCACCAGACTGGTGATCCTCAACACTCCGCATAACCCCAGTGCAACTGTCTGGCAGCAGGCTGATTTCGCCGCtt  >  2:121107/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       GGCAGGGGCGACGGAAGCGTTATACGCGGCGATTACCGCACTGGTGCGCAATGGCGATGAAGTGATTTGTTTTGATCCCAGCTATGACAGTTACGCCCCCGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACTGCAACCACCGCATTTTCGCGTTGACTGGCAGGAATTTGCCGCATTATTAAGCGAGCGCACCAGACTGGTGATCCTCAACACTCCGCATAACCCCAGTGCAACTGTCTGGCAGCAGGCTGATTTCGCCGCTT  >  NC_000913/633876‑634147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑