Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	865,084	T→G	I16L (ATC→CTC)	moeB ←	molybdopterin‑synthase adenylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	865,084	0	T	G	100.0%	188.5 / NA	52	I16L (ATC→CTC)	moeB	molybdopterin‑synthase adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (19/33);  total (19/33)

TCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGTTCTACTTCCACCCATTCGCCCACTTCCACATTGCCGCGATCGCGTTCCAGCACGATAAAGC  >  NC_000913/864955‑865220                                                                                                                                  |                                                                                                                                         tCAGGTTACCGACACCGGCGCTTGCCGGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGAGTTGCCGGTTg                                                                                                                                <  1:4522/140‑1 (MQ=255) tCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGAGTTGCCGGTTg                                                                                                                                <  2:1009432/140‑1 (MQ=255)     gTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGAGTTGCCGGTTGTAgc                                                                                                                            <  1:588944/140‑1 (MQ=255)          cGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGAGTTGCCGGTTGTAGCgcagc                                                                                                                       <  1:99933/140‑1 (MQ=255)          cGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGAGTTGCCGGTTGTAGCgcagc                                                                                                                       >  1:1253280/1‑140 (MQ=255)              acCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGAGTTGCCGGTTGTAGCGCAGCAtct                                                                                                                   >  2:991340/1‑140 (MQ=255)               ccGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGAGTTGCCGGTTGTAGCGCAGCAtctc                                                                                                                  <  2:1062689/140‑1 (MQ=255)               ccGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGAGTTGCCGGTTGTAGCGCAGCAtctc                                                                                                                  >  2:541718/1‑140 (MQ=255)                cGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGAGTTGCCGGTTGTAGCGCAGCATCTcc                                                                                                                 >  2:1104676/1‑140 (MQ=255)                 ggCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGAGTTGCCGGTTGTAGCGCAGCATCTCCt                                                                                                                <  1:851977/140‑1 (MQ=255)                      ttGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGAGTTGCCGGTTGTAGCGCAGCATCTCCTGATCg                                                                                                           <  2:676099/140‑1 (MQ=255)                       tGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGAGTTGCCGGTTGTAGCGCAGCATCTCCTGATCGc                                                                                                          >  1:1045570/1‑140 (MQ=255)                        gCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGCGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGAGTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCt                                                                                                         >  2:909368/1‑140 (MQ=255)                              tACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGAGTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTc                                                                                                   >  2:990964/1‑140 (MQ=255)                                 tGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGAGTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGc                                                                                                >  1:268002/1‑140 (MQ=255)                                 cgCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGAGTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGc                                                                                                <  2:968103/139‑1 (MQ=255)                                      ggCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGAGTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTa                                                                                           <  2:268002/140‑1 (MQ=255)                                        cTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGAGTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACa                                                                                         <  2:157123/140‑1 (MQ=255)                                        cTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGAGTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACa                                                                                         <  1:487312/140‑1 (MQ=255)                                        cTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGAGTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACa                                                                                         <  1:1029607/140‑1 (MQ=255)                                        cTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGAGTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACa                                                                                         <  1:1104676/140‑1 (MQ=255)                                          gCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGAGTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAgg                                                                                       <  2:815566/140‑1 (MQ=255)                                                   aGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGAGTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGaac                                                                              >  2:481521/1‑140 (MQ=255)                                                    ggCCGCCCAGGCCCACTATCAGCACGGGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGAGTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGaaca                                                                             <  2:1061991/140‑1 (MQ=255)                                                    ggCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGAGTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGaaca                                                                             <  1:319191/140‑1 (MQ=255)                                                    ggCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGAGTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGaaca                                                                             <  1:546839/140‑1 (MQ=255)                                                    ggCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGAGTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGaaca                                                                             <  1:909368/140‑1 (MQ=255)                                                          ccAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGAGTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGt                                                                       >  1:445600/1‑140 (MQ=255)                                                          ccAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGAGTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGt                                                                       >  2:438953/1‑140 (MQ=255)                                                          ccAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGAGTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGt                                                                       >  1:61015/1‑140 (MQ=255)                                                            aGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGAGTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTa                                                                     <  2:1045570/140‑1 (MQ=255)                                                              gCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGAGTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTaaa                                                                   <  2:1088455/140‑1 (MQ=255)                                                               cccACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGAGTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAAc                                                                  <  1:990964/140‑1 (MQ=255)                                                                    tATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGAGTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGTTc                                                             <  1:1217318/140‑1 (MQ=255)                                                                    tATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGAGTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGTTc                                                             <  1:541718/140‑1 (MQ=255)                                                                    tATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGAGTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGTTc                                                             >  1:970641/1‑140 (MQ=255)                                                                            cgcgAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGAGTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGTTCTACTTCCa                                                     <  2:970641/140‑1 (MQ=255)                                                                             gcgAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGAGTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGTTCTACTTCCAc                                                    >  1:810602/1‑140 (MQ=255)                                                                                agaATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGAGTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGTTCTACTTCCACCCa                                                 >  2:1211636/1‑140 (MQ=255)                                                                                 gaATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGAGTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGTTCTACTTCCACCCAt                                                <  1:504381/140‑1 (MQ=255)                                                                                      tttCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGAGTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGTTCTACTTCCACCCATTCGcc                                           <  2:563981/140‑1 (MQ=255)                                                                                      tttCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGAGTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGTTCTACTTCCACCCATTCGcc                                           <  2:226787/140‑1 (MQ=255)                                                                                              cctcctGGCCGTCAAAATCAAAGCCGCGCAGAATGAGTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGTTCTACTTCCACCCATTCGCCCACTTCca                                   >  2:1001540/1‑140 (MQ=255)                                                                                                            aaaTCAAAGCCGCGCAGAATGAGTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGTTCTACTTCCACCCATTCGCCCACTTCCACATTGCCGCGATcg                     <  2:1253280/140‑1 (MQ=255)                                                                                                                    gCCGCGCAGAATGAGTTGCCGGTTGTAGCACAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGTTCTACTTCCACCCATTCGCCCACTTCCACATTGCCGCGATCGCGTTCCAg             <  1:517169/140‑1 (MQ=255)                                                                                                                     ccGCGCAGAATGAGTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGTTCTACTTCCACCCATTCGCCCACTTCCACATTGCCGCGATCGCGTTCCAGc            <  1:759092/140‑1 (MQ=255)                                                                                                                      cgcgCAGAATGAGTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGTTCTACTTCCACCCATTCGCCCACTTCCACATTGCCGCGATCGCGTTCCAGCa           <  1:991340/140‑1 (MQ=255)                                                                                                                        cgcAGAATGAGTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGTTCTACTTCCACCCATTCGCCCACTTCCACATTGCCGCGATCGCGTTCCAGCACg         >  1:887643/1‑140 (MQ=255)                                                                                                                        cgcAGAATGAGTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGTTCTACTTCCACCCATTCGCCCACTTCCACATTGCCGCGATCGCGTTCCAGCACg         >  2:816585/1‑140 (MQ=255)                                                                                                                             aaTGAGTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGTTCTACTTCCATCCATTCGCCCACTTCCACATTGCCGCGATCGCGTTCCAGCACGATaaa    >  2:833407/1‑140 (MQ=255)                                                                                                                              aTGAGTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGTTCTACTTCCACCCATTCGCCCACTTCCACATTGCCGCGATCGCGTTCCAGCACGATAAAg   <  1:1134349/140‑1 (MQ=255)                                                                                                                               tGAGTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGTTCTACTTCCACCCATTCGCCCACTTCCACATTGCCGCGATCGCGTTCCAGCACGATAAAGc  <  1:438953/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                         TCAGGTTACCGACACCGGCGCTTGCCAGATACTGCGAGGCTGCACAGCCGAGGCCGCCCAGGCCCACTATCAGCACGCGAGAATCTTTCAGCGCCTCCTGGCCGTCAAAATCAAAGCCGCGCAGAATGATTTGCCGGTTGTAGCGCAGCATCTCCTGATCGCTGAGTTCCGCCATTACAGGCCTCCGAACAACGCGTTAAACGGTTCTACTTCCACCCATTCGCCCACTTCCACATTGCCGCGATCGCGTTCCAGCACGATAAAGC  >  NC_000913/864955‑865220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑