Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,604,3440CT47.6% 53.1 / 61.4 83S62F (TCT→TTT) bglJDNA‑binding transcriptional regulator BglJ
Reads supporting (aligned to +/- strand):  ref base C (10/33);  new base T (39/0);  total (50/33)
Fisher's exact test for biased strand distribution p-value = 2.87e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 104 total aligned reads displayed.

ATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAT  >  NC_000913/4604207‑4604481
                                                                                                                                          |                                                                                                                                          
aTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑tttttctt                                                                                                                                          >  1:1035536/1‑140 (MQ=255)
aTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑tttttctt                                                                                                                                          <  1:343058/140‑1 (MQ=255)
  gCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑tttttttttt                                                                                                                                        >  1:1674199/1‑140 (MQ=255)
  gCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑tttttctttt                                                                                                                                        >  1:331446/1‑140 (MQ=255)
  gCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑tttttctttt                                                                                                                                        >  1:1404162/1‑140 (MQ=255)
  gCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGAAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGACATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGG‑CAtctttttctttt                                                                                                                                        >  1:862407/1‑140 (MQ=255)
     tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑Tttttctttttct                                                                                                                                     <  2:659429/140‑1 (MQ=255)
     tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑Tttttctttttct                                                                                                                                     <  1:296684/140‑1 (MQ=255)
     tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑Tttttctttttct                                                                                                                                     <  1:705649/140‑1 (MQ=255)
     tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑Tttttctttttct                                                                                                                                     <  2:1245653/140‑1 (MQ=255)
              gtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATgaga                                                                                                                            <  2:120443/140‑1 (MQ=255)
                 tCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATCCCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGt                                                                                                                         <  2:458140/140‑1 (MQ=255)
                    gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTTTTCCATGAGAAGTGAg                                                                                                                      >  2:587101/1‑140 (MQ=255)
                    gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTTTGCCCTGAGGAGTGAg                                                                                                                      >  2:1067923/1‑140 (MQ=255)
                        tGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCa                                                                                                                  <  2:1534589/140‑1 (MQ=255)
                         gagaGTTTATTCAGAAAGTTTGCGGGTTACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTTGT‑TTTTTCTTTTTCTGCGCCGGGAAGTGGgcgccg                                                                                                                 >  2:59549/1‑138 (MQ=255)
                         gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGCTCTCGTTTGCGGCGGTGAT‑TTTTTCTTTTTTTTCCATCAGGAGTgtgtccag                                                                                                                 >  1:123062/1‑133 (MQ=255)
                         gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTTTTCCCTGAGAAGGGAGGGCag                                                                                                                 >  2:1302227/1‑140 (MQ=255)
                         gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTTTTCCATGAGAAGTGAGCGCag                                                                                                                 >  2:1548117/1‑140 (MQ=255)
                         gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTTTGCCCTGAGAAGGGAGGGCag                                                                                                                 >  1:337443/1‑140 (MQ=255)
                         gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTTTGCCATGGGAAGTGGGCGCag                                                                                                                 >  1:508900/1‑140 (MQ=255)
                         gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTTTGCCATGAGAAGTGAGGGCag                                                                                                                 >  1:308391/1‑140 (MQ=255)
                         gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTTTTCCATGAGAAGTGAGCGCag                                                                                                                 >  1:248/1‑140 (MQ=255)
                         gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGGGAGCGCag                                                                                                                 >  1:1381984/1‑140 (MQ=255)
                          agagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTTTTCCCTGAGGAGGGAGGGCaga                                                                                                                >  1:1507690/1‑140 (MQ=255)
                           gagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagag                                                                                                               <  1:355136/140‑1 (MQ=255)
                            agTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCT‑TTTTTCTTTTTTTTCCATGAGGAGTGAGGGCagaga                                                                                                              >  2:474084/1‑140 (MQ=255)
                               ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGTATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAggg                                                                                                           >  2:731575/1‑140 (MQ=255)
                               ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTTTTCCATGAGAAGTGAGGGGAGAGAggg                                                                                                           >  2:272256/1‑140 (MQ=255)
                               ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTTTGCCATGGGAAGTGAGGGCagggaggg                                                                                                           >  2:456047/1‑140 (MQ=255)
                               ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAggg                                                                                                           <  2:1058269/140‑1 (MQ=255)
                               ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAggg                                                                                                           <  1:788372/140‑1 (MQ=255)
                               ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAggg                                                                                                           >  1:1652224/1‑140 (MQ=255)
                                 aTTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCT‑TTTTTTTTTTTTTGCCATGAGAAGGGAGCGCAGgggggggt                                                                                                         >  1:1004402/1‑138 (MQ=255)
                                   tCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTa                                                                                                       <  2:1652224/140‑1 (MQ=255)
                                      gAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGGTTATCt                                                                                                    >  1:1492352/1‑140 (MQ=255)
                                      gAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTCTTCCCTGAGAAGTGAGCGCAGAGAGGGATTATCt                                                                                                    >  1:872379/1‑140 (MQ=255)
                                        aaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTg                                                                                                  <  2:331446/140‑1 (MQ=255)
                                        aaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTg                                                                                                  <  2:1338771/140‑1 (MQ=255)
                                           tttGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTTTGCCCTGAGAAGTGAGCGCAGAGAGGGGTTATTCTGccc                                                                                               >  1:15353/1‑139 (MQ=255)
                                                 ggTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCctgactga                                                                                         <  2:745032/140‑1 (MQ=255)
                                                  gTAACCCTTATAAGCTCCATACCTATTCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGGCTGaa                                                                                        <  1:1034505/140‑1 (MQ=255)
                                                     aCCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTTTTCCCTTAGAAGGGAGCGCAGAGGGGGGTTATTTTTGCTGACTGAAATg                                                                                     >  1:1617130/1‑140 (MQ=255)
                                                     aCCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTTTTCCATGAGAAGGGGGGGCAGAGAGGGGTTTTTTTTCCTGACTGAACTg                                                                                     >  1:1291608/1‑140 (MQ=255)
                                                     aCCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGGTTATCTTTCCTGACTGAACTg                                                                                     >  1:181414/1‑140 (MQ=255)
                                                     aCCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTCTGCCATGAGAAGGGAGGGCAGAGAGGGGTTATTTTTCCTGGCTGAACTg                                                                                     >  1:658118/1‑140 (MQ=255)
                                                     aCCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTg                                                                                     <  2:354443/140‑1 (MQ=255)
                                                            taAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTa                                                                             <  2:1285427/140‑1 (MQ=255)
                                                               gCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGt                                                                          <  2:392732/140‑1 (MQ=255)
                                                                 tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCT‑TTTTTTTTTTTTTGCCATTGGAAGGGAGCGCGGAGAGGGGTTATCTTTGCTGACTGAACTGGCGA‑TTAAGgtt                                                                        >  2:29421/1‑140 (MQ=255)
                                                                 tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTTTTCCATTAGAAGGGAGCGCAGAGGGGGATTATCTTTCCTGACTGAACTGGCGG‑TTaaagtt                                                                        >  2:1715417/1‑136 (MQ=255)
                                                                 tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTTTTCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGttt                                                                        >  2:755363/1‑140 (MQ=255)
                                                                 tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTTTGCCCTGAGAAAGGAGGGCAGGGGGGGGGTTTTTTTGCTGACTGAAATGGCGG‑TTAAGttt                                                                        >  2:1343695/1‑140 (MQ=255)
                                                                 tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGttt                                                                        >  1:1095806/1‑140 (MQ=255)
                                                                 tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCAT‑TTTTTTTTTTTTTGCCATGAGAGGTGAGGGCAGAGAGGGGTTAATTTTCCTGACTGAAATAGGGATTTAAGtt                                                                         >  2:161714/1‑140 (MQ=255)
                                                                  ccATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCT‑TTTTTCTTTTTTTGCCATGAGAAGAGAGCGCCGCGAGGGATTATCTTTCCCGACTGAAATGGCGC‑TTAAGtttt                                                                       >  2:535658/1‑139 (MQ=255)
                                                                     tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCcgc                                                                    >  1:998419/1‑140 (MQ=255)
                                                                         tataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTAc                                                                <  1:367648/140‑1 (MQ=255)
                                                                         tataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTAc                                                                <  1:1249400/140‑1 (MQ=255)
                                                                          ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTTTTCCCTGAGAAGGGAGCGCCGAGAGGGGTTATCTTTCCTGACTGAACTGGCGG‑TTAAGATTTCGCGTAcc                                                               >  2:1359572/1‑140 (MQ=255)
                                                                          ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTTTGCCATGAGAAGGGAGCGCAGAGAGGGGTTATTTTTCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTAcc                                                               >  2:1013080/1‑140 (MQ=255)
                                                                          ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTAcc                                                               >  1:1002149/1‑140 (MQ=255)
                                                                           taCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTTTGCCCTGGGAAGTGAGCGCAGAGGGGGGTTATTTTTTCTGACTGAACTGGCGA‑TTAAGTTTTCGCGGAccc                                                              >  2:307323/1‑140 (MQ=255)
                                                                           taCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCAT‑TTTTTTCTTTTCTTCCCTACGAAGAGAGCGCCGAGAGGGGTTATCCTTCCCCAGTTGAATGGCGA‑TTAAGTTTCcccgctccc                                                              >  2:576789/1‑135 (MQ=255)
                                                                            ctcAGTCAGGAGTCATTTCAGGATGCCATGTCGAGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTTCCCg                                                             <  2:1260129/138‑1 (MQ=255)
                                                                            aCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCg                                                             <  1:1135494/140‑1 (MQ=255)
                                                                            aCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCg                                                             <  1:580099/140‑1 (MQ=255)
                                                                               aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTTTGCCCTTAGAAGGGAGGGCAGGGGGGGGTTATTTTGCCTGACTGAACTGGCGA‑TTTAGTTTTCGCGGACCCGGCg                                                          >  2:1093294/1‑140 (MQ=255)
                                                                               aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTTTGCCCTGAGAAGTGAGGGCAGAGAGGGGTTTTCTTTCCTTACTGAAATTGCGG‑TTAAGTTTTCCCGTAccccacg                                                          >  2:542533/1‑136 (MQ=255)
                                                                                gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTTTGCCCTGAGAAGTGAGCGCAGAGAGGGGTTATCTTTCCTGACTGAACTGGCGA‑TTAAGTTTTCGCGTACCCGGCGt                                                         >  2:1610256/1‑140 (MQ=255)
                                                                                 tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTTAT‑TTTTTTTTTTTTTGCCAAGAGGAATGAGCGCAGGGAGGGATTATCTTGCCTGAATGAGCTGGGGG‑TTAAGTTTTCGCGGACCCCGGGtt                                                        >  2:1566641/1‑140 (MQ=255)
                                                                                  cAGGTGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTTTTCCATGAGAAGGGAGCGCAGAGGGGGGTTATTTTTCCTGACTGAACTGGCGA‑TTTAGTTTCCGCGTACCCGGCGgtt                                                       >  2:61805/1‑140 (MQ=255)
                                                                                  cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGttt                                                       >  1:918993/1‑140 (MQ=255)
                                                                                  cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTTTGCCATGAGAAGGGAGCGCAGAGAGGGGTTATCTTTCCTGACTGAACTGGCGG‑TTTAGTTTTCCCGTACCCGGCGttt                                                       >  2:1099818/1‑140 (MQ=255)
                                                                                   aGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTTTGCCCTGAGAAGGGAGGGCAGGGAGGGGTTATCTTGGCTGACTGGACTGGCGA‑TTAAGTTTTCGCGTACCCCGCGgttt                                                      >  2:1346541/1‑139 (MQ=255)
                                                                                        tCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTAt                                                 <  2:1404162/140‑1 (MQ=255)
                                                                                          aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTTTGCCCTGAGAAGGGAGCGCCGAGGGGGGTTATCTTTCCTGACTTGACTGGCGG‑TTAAGTTTTCGCGTACCCCGCGGTTAGTTAttt                                               >  2:167969/1‑139 (MQ=255)
                                                                                               aGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgat                                          <  1:801652/140‑1 (MQ=255)
                                                                                                  aTGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgat                                       <  1:1485914/140‑1 (MQ=255)
                                                                                                  aTGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgat                                       <  1:1151099/140‑1 (MQ=255)
                                                                                                  aTGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgat                                       <  1:1302227/140‑1 (MQ=255)
                                                                                                  aTGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgat                                       <  2:1414540/140‑1 (MQ=255)
                                                                                                    gCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTCTGCCATGAGAAGTGAGCGCAGGGAGGGATTATCTTGCCTGACTGAACTGGCGG‑TTAAGTTTCCGCGTACCCGGCGGTTTGTTATTTCGggtgatga                                     >  2:124405/1‑140 (MQ=255)
                                                                                                      cATGTCGCGGATCTCGTTTGCGGCGGTCCT‑TTTTTTTTTTTTTGGCCCGAGAAAGGAGCGCCGAGAGGGGGTTTTTTTGCTGACTGAAACGGCGA‑TTTAATTTTCACCAACCCGGCGGTTTATTTTTGCGGGTGGGGata                                   >  2:664627/1‑140 (MQ=255)
                                                                                                             cgGATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGc                            >  2:1685011/1‑140 (MQ=255)
                                                                                                             cgGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGc                            <  2:1617130/140‑1 (MQ=255)
                                                                                                              ggATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCt                           <  1:1099818/140‑1 (MQ=255)
                                                                                                              ggATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCt                           <  2:1507690/140‑1 (MQ=255)
                                                                                                               gATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTCTTCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGGTTCCGCGTACCCGGCGTTTAGTTATTTCGGATGATGATATTGAAACTc                          >  2:559491/1‑140 (MQ=255)
                                                                                                                      tttGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGAt                   <  2:337443/140‑1 (MQ=255)
                                                                                                                      tttGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGAt                   <  1:61805/140‑1 (MQ=255)
                                                                                                                        tGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTg                 <  1:1079789/140‑1 (MQ=255)
                                                                                                                          cggcggTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGt               <  1:29421/140‑1 (MQ=255)
                                                                                                                          cggcggTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGt               <  2:725228/140‑1 (MQ=255)
                                                                                                                             cggTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCa            <  1:662243/140‑1 (MQ=255)
                                                                                                                               gTCCT‑TTTTTTTTTTTTTGCCATGAGAAGGGAGCGCAGGGAGGGATTATCTTGCCTGACTGAACTGGCGG‑TTTAGTTTTCGCGGACCCGGGGTTTTGGTATTTCGGGTGGTGATATTGAAGGTTGGCTTGTTTGTTCAtt          >  2:47009/1‑140 (MQ=255)
                                                                                                                                        ttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAt  <  2:791603/140‑1 (MQ=255)
                                                                                                                                        ttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAt  <  2:1095806/140‑1 (MQ=255)
                                                                                                                                        ttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAt  <  1:456047/140‑1 (MQ=255)
                                                                                                                                        ttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAt  <  1:167969/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                          
ATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAT  >  NC_000913/4604207‑4604481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑