Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,638,720	(A)8→7	intergenic (‑53/+310)	cspI ← / ← rzpQ	Qin prophage; cold shock protein CspI/Qin prophage; DUF2514 domain‑containing protein RzpQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,638,713	0	A	.	100.0%	340.0 / NA	79	intergenic (‑46/+317)	cspI/rzpQ	Qin prophage; cold shock protein CspI/Qin prophage; DUF2514 domain‑containing protein RzpQ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (39/40);  total (39/40)

AAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAATTTGTAACAAACCAGAACACCATTAACGCATGATTAACCACCCATAGCA  >  NC_000913/1638575‑1638846                                                                                                                                           |                                                                                                                                      aaaCACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGaa                                                                                                                                      <  2:999981/140‑1 (MQ=255) aaaCACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGaa                                                                                                                                      >  1:1415263/1‑140 (MQ=255)   acacATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGaaaa                                                                                                                                    >  1:698501/1‑140 (MQ=255)       aTCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGaaaaaaat                                                                                                                                >  2:714012/1‑139 (MQ=255)       aTCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGaaaaaaat                                                                                                                                >  2:1188702/1‑139 (MQ=255)        tCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGaaaaaaata                                                                                                                               >  2:205978/1‑138 (MQ=255)            tgctgcCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCag                                                                                                                          <  1:221424/140‑1 (MQ=255)              ctgcCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCagag                                                                                                                        >  2:951855/1‑140 (MQ=255)                   aTCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGaaa                                                                                                                   >  2:697643/1‑140 (MQ=255)                   aTCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGaaa                                                                                                                   >  2:1005692/1‑140 (MQ=255)                        tCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACca                                                                                                              <  2:1317731/140‑1 (MQ=255)                         cGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAGCCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACcaa                                                                                                             <  1:852030/140‑1 (MQ=255)                         cGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACcaa                                                                                                             >  2:1499743/1‑140 (MQ=255)                               gatgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGa                                                                                                       <  2:59043/140‑1 (MQ=255)                               gatgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGa                                                                                                       >  2:7971/1‑140 (MQ=255)                               gatgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGa                                                                                                       >  1:182040/1‑140 (MQ=255)                                atgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGaa                                                                                                      <  2:1206843/140‑1 (MQ=255)                                     aCCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAAtctc                                                                                                 >  1:1396090/1‑140 (MQ=255)                                       cAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGTAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCaa                                                                                               <  2:634503/140‑1 (MQ=255)                                       cAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCaa                                                                                               <  2:950903/140‑1 (MQ=255)                                       cAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCaa                                                                                               <  2:33619/140‑1 (MQ=255)                                               ttttCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACa                                                                                       <  1:205978/140‑1 (MQ=255)                                               ttttCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACa                                                                                       <  1:1487745/140‑1 (MQ=255)                                                    aGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAaataa                                                                                  <  1:7971/140‑1 (MQ=255)                                                     gggTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAaataat                                                                                 <  2:1001130/140‑1 (MQ=255)                                                      ggTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAataata                                                                                >  1:1008453/1‑140 (MQ=255)                                                       gTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAAtaataa                                                                               >  2:1496242/1‑140 (MQ=255)                                                             ccATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATtat                                                                         <  2:827453/140‑1 (MQ=255)                                                                tttCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAAc                                                                      >  2:24378/1‑140 (MQ=255)                                                                tttCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAAc                                                                      >  2:1448733/1‑140 (MQ=255)                                                                tttCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAAc                                                                      >  2:909204/1‑140 (MQ=255)                                                                tttCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAAc                                                                      >  2:931079/1‑140 (MQ=255)                                                                tttCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAAc                                                                      >  2:955233/1‑140 (MQ=255)                                                                tttCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAAc                                                                      >  2:1548384/1‑140 (MQ=255)                                                                 ttCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACa                                                                     <  1:1499743/140‑1 (MQ=255)                                                                     cTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGa                                                                 <  2:604673/140‑1 (MQ=255)                                                                     cTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGa                                                                 <  2:525816/140‑1 (MQ=255)                                                                             gTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCa                                                         >  1:919639/1‑140 (MQ=255)                                                                              tCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAg                                                        >  1:494854/1‑140 (MQ=255)                                                                              tCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAg                                                        >  2:980337/1‑140 (MQ=255)                                                                              tCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAg                                                        >  1:1301782/1‑140 (MQ=255)                                                                                aTTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGAt                                                      <  1:931079/140‑1 (MQ=255)                                                                                  tttGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATaa                                                    >  1:1100885/1‑140 (MQ=255)                                                                                    tGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTTAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAAt                                                  <  1:1102404/140‑1 (MQ=255)                                                                                     gTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAAtt                                                 <  2:698501/140‑1 (MQ=255)                                                                                     gTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAAtt                                                 <  2:1420466/140‑1 (MQ=255)                                                                                     gTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAAtt                                                 <  2:393285/140‑1 (MQ=255)                                                                                     gTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAAtt                                                 <  1:833779/140‑1 (MQ=255)                                                                                     gTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAAtt                                                 <  2:965047/140‑1 (MQ=255)                                                                                     gTTAGACATAATTATTACCTTTTGAAGAAATTAACCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAAtt                                                 <  2:1415263/140‑1 (MQ=255)                                                                                          aCATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAATTTGTaa                                            >  2:938227/1‑140 (MQ=255)                                                                                          aCATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAATTTGTaa                                            <  1:1552050/140‑1 (MQ=255)                                                                                              aaTTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAATTTGTAACaaa                                        >  2:1241011/1‑140 (MQ=255)                                                                                                  attaCCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAATTTGTAACAAACCAg                                    <  2:1518803/140‑1 (MQ=255)                                                                                                   ttaCCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAATTTGTAACAAACCAGa                                   >  1:101776/1‑140 (MQ=255)                                                                                                     aCCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAATTTGTAACAAACCAGAac                                 >  1:612986/1‑140 (MQ=255)                                                                                                     aCCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAATTTGTAACAAACCAGAac                                 >  2:478387/1‑140 (MQ=255)                                                                                                     aCCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAATTTGTAACAAACCAGAac                                 <  1:1041691/140‑1 (MQ=255)                                                                                                        ttttGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAATTTGTAACAAACCAGAACAcc                              >  1:229946/1‑140 (MQ=255)                                                                                                        ttttGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAATTTGTAACAAACCAGAACAcc                              >  1:797126/1‑140 (MQ=255)                                                                                                        ttttGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAAACTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAATTTGTAACAAACCAGAACAcc                              >  1:365057/1‑140 (MQ=255)                                                                                                         tttGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAATTTGTAACAAACCAGAACACCa                             >  1:1191901/1‑140 (MQ=255)                                                                                                          ttGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAATTTGTAACAAACCAGAACACCAt                            >  2:242855/1‑140 (MQ=255)                                                                                                          ttGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAATTTGTAACAAACCAGAACACCAt                            >  1:1033619/1‑140 (MQ=255)                                                                                                          ttGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAATTTGTAACAAACCAGAACACCAt                            <  1:714012/140‑1 (MQ=255)                                                                                                          ttGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAATTTGTAACAAACCAGAACACCAt                            >  2:829990/1‑140 (MQ=255)                                                                                                          ttGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAATTTGTAACAAACCAGAACACCAt                            >  1:1155626/1‑140 (MQ=255)                                                                                                          ttGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAATTTGTAACAAACCAGAACACCAt                            >  2:195762/1‑140 (MQ=255)                                                                                                          ttGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAATTTGTAACAAACCAGAACACCAt                            >  1:1149979/1‑140 (MQ=255)                                                                                                          ttGAAGAAATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAATTTGTAACAAACCAGAACACCAt                            <  1:951855/140‑1 (MQ=255)                                                                                                              agaaATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAATTTGTAACAAACCAGAACACCATTAAc                        <  2:466269/140‑1 (MQ=255)                                                                                                               gaaATTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAATTTGTAACAAACCAGAACACCATTAACg                       <  2:138234/140‑1 (MQ=255)                                                                                                                 aaTTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAATTTGTAACAAACCAGAACACCATTAACGCa                     <  1:1448733/140‑1 (MQ=255)                                                                                                                  aTTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAATTTGTAACAAACCAGAACACCATTAACGCAt                    >  2:790950/1‑140 (MQ=255)                                                                                                                  aTTAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAATTTGTAACAAACCAGAACACCATTAACGCAt                    <  1:24378/140‑1 (MQ=255)                                                                                                                   ttAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAATTTGTAACAAACCAGAACACCATTAACGCATg                   <  1:896086/140‑1 (MQ=255)                                                                                                                   ttAGCCCTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAATTTGTAACAAACCAGAACACCATTAACGCATg                   <  2:774266/140‑1 (MQ=255)                                                                                                                         cTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAATTTGTAACAAACCAGAACACCATTAACGCATGATTAac             <  1:980337/140‑1 (MQ=255)                                                                                                                         cTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAATTTGTAACAAACCAGAACACCATTAACGCATGATTAac             <  2:706517/140‑1 (MQ=255)                                                                                                                         cTTGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAATTTGTAACAAACCAGAACACCATTAACGCATGATTAac             <  1:938227/140‑1 (MQ=255)                                                                                                                          ttGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAATTTGTAACAAACCAGAACACCATTAACGCATGATTAacc            <  2:1287424/140‑1 (MQ=255)                                                                                                                           tGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAATTTGTAACAAACCAGAACACCATTAACGCATGATTAacca           >  2:825692/1‑140 (MQ=255)                                                                                                                           tGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAATTTGTAACAAACCAGAACACCATTAACGCATGATTAacca           >  1:57034/1‑140 (MQ=255)                                                                                                                           tGGGCAGAATGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAATTTGTAACAAACCAGAACACCATTAACGCATGATTAacca           >  1:401458/1‑140 (MQ=255)                                                                                                                                    tGGTCCG‑AAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAATTTGTAACAAACCAGAACACCATTAACGCATGATTAACCACCCATAGCa  <  1:923958/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                      AAACACATCTTTGCTGCCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGAAAAAAAATATCAGAGAGAAAAACCAACAAGGAAATCTCAAGAGGTACAAATAATAAAATTATAACAATGACTGCTTCAGATAAATTTGTAACAAACCAGAACACCATTAACGCATGATTAACCACCCATAGCA  >  NC_000913/1638575‑1638846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑