BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,312,246	C→T	R249H (CGT→CAT)	alsR ←	DNA‑binding transcriptional repressor AlsR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,312,246	0	C	T	100.0%	754.5 / NA	212	R249H (CGT→CAT)	alsR	DNA‑binding transcriptional repressor AlsR
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (108/104); total (108/104)

Only 100 of 215 total aligned reads displayed.

CGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTA  >  NC_000913/4312110‑4312381

 gcgcCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGAcc                                                                                                                                     >  1:832095/1‑140 (MQ=255)

 gcgcCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGAcc                                                                                                                                     <  2:340421/140‑1 (MQ=255)

 gcgcCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGAcc                                                                                                                                     >  2:2221167/1‑140 (MQ=255)

  cgcCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCt                                                                                                                                    <  2:1909363/140‑1 (MQ=255)

   gcCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCta                                                                                                                                   >  2:1628668/1‑140 (MQ=255)

    ccTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCtaa                                                                                                                                  >  1:1403680/1‑140 (MQ=255)

     cTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCtaat                                                                                                                                 >  2:765403/1‑140 (MQ=255)

     cTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCtaat                                                                                                                                 >  2:2505089/1‑140 (MQ=255)

           agaagaAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGc                                                                                                                           <  1:979566/140‑1 (MQ=255)

           agaagaAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGc                                                                                                                           <  2:2041721/140‑1 (MQ=255)

           agaagaAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGc                                                                                                                           <  2:209809/140‑1 (MQ=255)

                  aTCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCg                                                                                                                    >  2:2175420/1‑140 (MQ=255)

                  aTCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGAAGTATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCg                                                                                                                    >  2:2320295/1‑140 (MQ=255)

                            gcgcCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGa                                                                                                          <  1:1214215/140‑1 (MQ=255)

                              gcCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGc                                                                                                        <  2:126949/140‑1 (MQ=255)

                              gcCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGc                                                                                                        <  2:2563183/140‑1 (MQ=255)

                               ccGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCa                                                                                                       <  2:240743/140‑1 (MQ=255)

                               ccGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCa                                                                                                       <  2:1686956/140‑1 (MQ=255)

                                         gCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAataatataa                                                                                             <  1:2509236/140‑1 (MQ=255)

                                              ttaatattaGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGc                                                                                        <  2:1570913/140‑1 (MQ=255)

                                              ttaatattaGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGc                                                                                        <  2:1164340/140‑1 (MQ=255)

                                              ttaatattaGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGc                                                                                        <  2:405434/140‑1 (MQ=255)

                                               taatattaGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGcc                                                                                       >  1:1042843/1‑140 (MQ=255)

                                                  tattaGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGt                                                                                    <  2:556742/140‑1 (MQ=255)

                                                      aGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCg                                                                                <  2:2630532/140‑1 (MQ=255)

                                                      aGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCg                                                                                >  2:1520324/1‑140 (MQ=255)

                                                      aGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCg                                                                                <  1:1482222/140‑1 (MQ=255)

                                                      aGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCg                                                                                >  1:1388998/1‑140 (MQ=255)

                                                      aGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCg                                                                                <  1:2494738/140‑1 (MQ=255)

                                                      aGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCg                                                                                >  2:1099281/1‑140 (MQ=255)

                                                      aGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCg                                                                                <  1:2111756/140‑1 (MQ=255)

                                                       gCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGc                                                                               >  2:348429/1‑140 (MQ=255)

                                                       gCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGc                                                                               >  1:2044560/1‑140 (MQ=255)

                                                       gCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGc                                                                               >  1:785068/1‑140 (MQ=255)

                                                        cTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCt                                                                              >  2:2356231/1‑140 (MQ=255)

                                                          tGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTAt                                                                            <  2:832095/140‑1 (MQ=255)

                                                           gTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGt                                                                                    <  2:2006060/131‑1 (MQ=255)

                                                           gTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGt                                                                                    >  1:2006060/1‑131 (MQ=255)

                                                           gTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATc                                                                           >  1:1183504/1‑140 (MQ=255)

                                                            ttCAATGTTGAGCTGGGCGATAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCGGTTTCGCTATCg                                                                          >  1:590762/1‑140 (MQ=255)

                                                            ttCAATGTTGAGCTGGGCGACAGATACAAAAAAAGCGCCCAGCAACGTTAGTTGTACTATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCTGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCg                                                                          >  1:2574913/1‑140 (MQ=255)

                                                            ttCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCg                                                                                <  2:1470433/134‑1 (MQ=255)

                                                            ttCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCg                                                                                >  1:1470433/1‑134 (MQ=255)

                                                            ttCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCg                                                                          >  2:877037/1‑140 (MQ=255)

                                                            ttCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCg                                                                          >  1:1702499/1‑140 (MQ=255)

                                                            ttCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCg                                                                          >  2:1363002/1‑140 (MQ=255)

                                                            ttCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCg                                                                          <  2:2452681/140‑1 (MQ=255)

                                                            ttCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCg                                                                          >  2:2476846/1‑140 (MQ=255)

                                                            ttCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCg                                                                          >  2:367861/1‑140 (MQ=255)

                                                            ttCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCg                                                                          >  2:631374/1‑140 (MQ=255)

                                                            ttCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCg                                                                          >  1:189426/1‑140 (MQ=255)

                                                             tCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCgg                                                                         >  2:121367/1‑140 (MQ=255)

                                                              cAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGt                                                                        <  1:1305353/140‑1 (MQ=255)

                                                              cAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGt                                                                        >  2:1755544/1‑140 (MQ=255)

                                                               aaTGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTg                                                                       <  2:581096/140‑1 (MQ=255)

                                                                 tGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGaa                                                                     <  1:2361543/140‑1 (MQ=255)

                                                                  gTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGaa                                                                     <  2:479831/139‑1 (MQ=255)

                                                                   ttGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATg                                                                   <  1:1520324/140‑1 (MQ=255)

                                                                    tGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATgg                                                                  <  1:2004379/140‑1 (MQ=255)

                                                                    tGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATgg                                                                  <  1:2221167/140‑1 (MQ=255)

                                                                    tGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATgg                                                                  <  2:1534581/140‑1 (MQ=255)

                                                                      aGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTa                                                                >  1:1442254/1‑140 (MQ=255)

                                                                         tGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCt                                                             <  1:461389/140‑1 (MQ=255)

                                                                         tGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCt                                                             <  1:631374/140‑1 (MQ=255)

                                                                         tGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCt                                                             <  1:1112573/140‑1 (MQ=255)

                                                                         tGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCt                                                             <  1:2288701/140‑1 (MQ=255)

                                                                           ggCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTAt                                                           >  1:2581637/1‑140 (MQ=255)

                                                                           ggCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTAt                                                           >  1:2466563/1‑140 (MQ=255)

                                                                           ggCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTAt                                                           >  1:564405/1‑140 (MQ=255)

                                                                           ggCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTAt                                                           >  2:2136458/1‑140 (MQ=255)

                                                                           ggCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTAt                                                           >  1:1018155/1‑140 (MQ=255)

                                                                           ggCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTAt                                                           >  1:1593229/1‑140 (MQ=255)

                                                                             cGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATgg                                                         >  2:1131454/1‑140 (MQ=255)

                                                                              gACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATggg                                                        <  2:785068/140‑1 (MQ=255)

                                                                                cAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATgg                                                                  >  1:2000690/1‑128 (MQ=255)

                                                                                 agagACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTta                                                     <  1:765403/140‑1 (MQ=255)

                                                                                 agagACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTta                                                     <  2:1435304/140‑1 (MQ=255)

                                                                                               cGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCttt                                       >  2:1051735/1‑140 (MQ=255)

                                                                                                                tGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGc                      <  2:2121988/140‑1 (MQ=255)

                                                                                                                tGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGc                      >  1:2150790/1‑140 (MQ=255)

                                                                                                                tGTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGc                      >  1:265484/1‑140 (MQ=255)

                                                                                                                 gTAATATTCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGcc                     <  2:1409971/140‑1 (MQ=255)

                                                                                                                       ttCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCt               >  1:360018/1‑140 (MQ=255)

                                                                                                                       ttCTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCt               >  1:1534982/1‑140 (MQ=255)

                                                                                                                         cTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTAc             >  1:1201215/1‑140 (MQ=255)

                                                                                                                         cTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTAc             >  1:1857259/1‑140 (MQ=255)

                                                                                                                         cTTGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTAc             >  1:224730/1‑140 (MQ=255)

                                                                                                                          ttGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACg            >  1:1571612/1‑140 (MQ=255)

                                                                                                                           tGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACgg           <  1:1434059/140‑1 (MQ=255)

                                                                                                                           tGCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACgg           <  2:224730/140‑1 (MQ=255)

                                                                                                                            gCCGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGc          <  2:200129/140‑1 (MQ=255)

                                                                                                                             ccGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGcc         <  1:1755544/140‑1 (MQ=255)

                                                                                                                             ccGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGcc         <  1:163267/140‑1 (MQ=255)

                                                                                                                             ccGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGcc         >  2:1728179/1‑140 (MQ=255)

                                                                                                                             ccGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGcc         <  2:2380557/140‑1 (MQ=255)

                                                                                                                             ccGAGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGcc         <  2:2581637/140‑1 (MQ=255)

                                                                                                                                aGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCt      >  2:1979693/1‑140 (MQ=255)

                                                                                                                                aGGCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCt      >  2:858493/1‑140 (MQ=255)

                                                                                                                                 ggCATTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCtt     >  2:140322/1‑140 (MQ=255)

                                                                                                                                    aTTATGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTa  <  2:1941652/140‑1 (MQ=255)

CGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTA  >  NC_000913/4312110‑4312381

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`