Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	223,759	C→T	intergenic (+351/‑12)	gmhB → / → rrsH	D‑glycero‑beta‑D‑manno‑heptose‑1,7‑bisphosphate 7‑phosphatase/16S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	223,759	0	C	T	100.0%	151.1 / NA	66	intergenic (+351/‑12)	gmhB/rrsH	D‑glycero‑beta‑D‑manno‑heptose‑1,7‑bisphosphate 7‑phosphatase/16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/30);  total (36/30)

CTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGCATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTGACGAGTGGCGGAC  >  NC_000913/223629‑223880                                                                                                                                   |                                                                                                                          ctctTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACtt                                                                                                                  >  1:114760/1‑140 (MQ=17)   ctTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACtttt                                                                                                                >  1:934165/1‑140 (MQ=17)         cAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTg                                                                                                          <  1:651512/140‑1 (MQ=18)          aaTTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTgt                                                                                                         <  1:754748/140‑2 (MQ=18)           aTTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGaa                                                                                                        >  2:757514/1‑140 (MQ=17)            tttATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAag                                                                                                       >  1:666305/1‑140 (MQ=17)                 cAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGttt                                                                                                  <  1:424916/140‑1 (MQ=18)                 cAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGttt                                                                                                  <  1:772582/140‑1 (MQ=17)                      aaTCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCa                                                                                             >  1:775204/1‑140 (MQ=17)                          tgtgtgGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGc                                                                                         <  1:923655/140‑1 (MQ=17)                           gtgtgGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCt                                                                                        <  1:707044/140‑1 (MQ=18)                            tgtgGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTc                                                                                       <  2:273589/140‑1 (MQ=18)                            tgtgGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTc                                                                                       <  2:114760/140‑1 (MQ=18)                               gggCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGa                                                                                    >  2:165046/1‑140 (MQ=17)                                  cACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTg                                                                                 >  2:568012/1‑140 (MQ=17)                                  cACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTg                                                                                 >  1:681956/1‑140 (MQ=17)                                  cACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTg                                                                                 >  2:808404/1‑140 (MQ=17)                                    cTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGaa                                                                               >  2:665378/1‑140 (MQ=17)                                    cTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGaa                                                                               >  2:183222/1‑140 (MQ=17)                                     tCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAAc                                                                              >  1:639031/1‑140 (MQ=17)                                      cGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACg                                                                             >  2:4091/1‑140 (MQ=17)                                             aCGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTggcgg                                                                      <  2:666305/140‑1 (MQ=17)                                             aCGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTggcgg                                                                      >  2:940561/1‑140 (MQ=17)                                              cGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTggcggc                                                                     <  2:620875/140‑1 (MQ=17)                                              cGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTggcggc                                                                     <  1:407021/140‑1 (MQ=17)                                              cGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTggcggc                                                                     <  2:214383/140‑1 (MQ=17)                                               ggATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCa                                                                    >  1:247808/1‑140 (MQ=17)                                               ggATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCa                                                                    >  2:351259/1‑140 (MQ=17)                                                 aTTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAgg                                                                  >  1:330935/1‑140 (MQ=17)                                                   tCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGcc                                                                >  1:559280/1‑140 (MQ=17)                                                    cTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCt                                                               <  1:287096/140‑1 (MQ=17)                                                     ttAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTa                                                              >  1:587560/1‑140 (MQ=17)                                                     ttAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTa                                                              >  1:380674/1‑140 (MQ=17)                                                      tAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTaa                                                             <  1:757514/140‑1 (MQ=17)                                                           tCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACAt                                                        >  1:872166/1‑140 (MQ=17)                                                           tCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGG‑‑CAGATTGAACGCTGGCGGCAGGCCTAACACATGc                                                      >  2:393089/1‑140 (MQ=255)                                                             gCAAGACGAAAAATGAATACCGAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTaa                                                             >  2:874875/1‑133 (MQ=12)                                                             gCAAGACGAAAAATGAATACCGAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTaa                                                             <  1:874875/133‑1 (MQ=17)                                                              cAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCa                                                     >  1:655996/1‑140 (MQ=17)                                                                 gACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGt                                                  <  1:897211/140‑1 (MQ=17)                                                                    gAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGa                                               <  1:165046/140‑1 (MQ=17)                                                                      aaaaTGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAAc                                             <  2:587560/140‑1 (MQ=17)                                                                        aaTGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGG‑‑CAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTa                                         <  1:393089/140‑1 (MQ=255)                                                                         aTGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGt                                          <  1:118630/140‑1 (MQ=17)                                                                            aaTACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAAc                                       <  2:330935/140‑1 (MQ=17)                                                                            aaTACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAAc                                       <  2:639031/140‑1 (MQ=17)                                                                                      ctcAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAgc                             >  1:722589/1‑140 (MQ=18)                                                                                            agTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAgcttgctt                       >  2:189838/1‑140 (MQ=17)                                                                                               gAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGcttctt                    >  1:80316/1‑140 (MQ=17)                                                                                               gAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGcttctt                    >  1:495314/1‑140 (MQ=18)                                                                                               gAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGcttctt                    >  1:548311/1‑140 (MQ=18)                                                                                               gAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGcttct                     <  1:760702/139‑1 (MQ=17)                                                                                               gAACACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGcttct                     >  2:760702/1‑139 (MQ=18)                                                                                                aaCACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCttt                   >  2:90485/1‑140 (MQ=17)                                                                                                aaCACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCttt                   >  1:53713/1‑140 (MQ=18)                                                                                                aaCACGTAATTCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCttt                   >  2:289906/1‑140 (MQ=18)                                                                                                         ttCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTGACGAg          <  2:681956/140‑1 (MQ=17)                                                                                                         ttCATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTGACGAg          <  1:940561/140‑1 (MQ=17)                                                                                                           cATTACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTGACGAGTg        <  2:548311/140‑1 (MQ=17)                                                                                                             ttACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTGACGAGTGGc      >  2:491074/1‑140 (MQ=18)                                                                                                              tACAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTGACGAGTGGCg     <  2:247808/140‑1 (MQ=17)                                                                                                               aCAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTGACGAGTGGCgg    >  1:416383/1‑140 (MQ=17)                                                                                                                cAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTGACGAGTGGCGGa   <  2:872166/140‑1 (MQ=14)                                                                                                                cAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTGACGAGTGGCGGa   <  2:722589/140‑1 (MQ=14)                                                                                                                cAAAGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTGACGAGTGGCGGa   <  2:380674/140‑1 (MQ=14)                                                                                                                 aaaGTTTAATTCTTTGAGTATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTGACGAGTGGCGGAc  >  2:581434/1‑140 (MQ=11)                                                                                                                                   |                                                                                                                          CTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAGATACGGATTCTTAACGTCGCAAGACGAAAAATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACAAAGTTTAATTCTTTGAGCATCAAACTTTTAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGAAGCTTGCTTCTTTGCTGACGAGTGGCGGAC  >  NC_000913/223629‑223880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑