Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,351,051	T→A	V204V (GTA→GTT)	dcuS ←	sensory histidine kinase DcuS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,351,051	0	T	A	100.0%	166.7 / NA	49	V204V (GTA→GTT)	dcuS	sensory histidine kinase DcuS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (24/25);  total (24/25)

CATCGTTGATCAGCGTGACCTCGCCGCGATCGTCCACGGCAACGACGCCTTCTTTGATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGC  >  NC_000913/4350915‑4351173                                                                                                                                         |                                                                                                                           cATCGTTGATCAGCGTGACCTCGCCGCGATCGTCCACGGCAACGACGCCTTCTTTGATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGAAcc                                                                                                                         <  1:215737/140‑1 (MQ=255) cATCGTTGATCAGCGTGACCTCGCCGCGATCGTCCACGGCAACGACGCCTTCTTTGATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGAAcc                                                                                                                         >  2:171075/1‑140 (MQ=255)              cGTGACCTCGCCGCGATCGTCCACGGCAACGACGCCTTCTTTGATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGAACCTTGACCAGAATGc                                                                                                            <  1:848351/140‑1 (MQ=255)              cGTGACCTCGCCGCGATCGTCCACGGCAACGACGCCTTCTTTGATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGAACCTTAACCAGAATGc                                                                                                            <  1:171075/140‑1 (MQ=255)              cGTGACCTCGCCGCGATCGTCCACGGCAACGACGCCTTCTTTGATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGAACCTTAACCAGAATGc                                                                                                            >  1:730793/1‑140 (MQ=255)              cGTGACCTCGCCGCGATCGTCCACGGCAACGACGCCTTCTTTGATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGAACCTTAACCAGAATGc                                                                                                            >  2:105399/1‑140 (MQ=255)              cGTGACCTCGCCGCGATCGTCCACGGCAACGACGCCTTCTTTGATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGAACCTTAACCAGAATGc                                                                                                            >  2:140077/1‑140 (MQ=255)              cGTGACCTCGCCGCGATCGTCCACGGCAACGACGCCTTCTTTGATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGAACCTTAACCAGAATGc                                                                                                            >  1:429991/1‑140 (MQ=255)                       gccgcGATCGTCCACGGCAACGACGCCTTCTTTGATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGAACCTTAACCAGAATGCAGGTGCCaa                                                                                                   <  1:315861/140‑1 (MQ=255)                        ccgcGATCGTCCACGGCAACGACGCCTTCTTTGATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGAACCTTAACCAGAATGCAGGTGCCaa                                                                                                   >  1:14379/1‑139 (MQ=255)                        ccgcGATCGTCCACGGCAACGACGCCTTCTTTGATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGAACCTTAACCAGAATGCAGGTGCCaa                                                                                                   <  2:14379/139‑1 (MQ=255)                              tCGTCCACGGCAACGACGCCTTCTTTGATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGAACCTTAACCAGAATGCAGGTGCCAATCAGccc                                                                                            >  1:800597/1‑140 (MQ=255)                                 tCCACGGCAACGACGCCTTCTTTGATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGAACCTTAACCAGAATGCAGGTGCCAATCAGTCCGAc                                                                                         >  2:953643/1‑140 (MQ=255)                                 tCCACGGCAACGACGCCTTCTTTGATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGAACCTTAACCAGAATGCAGGTGCCAATCAGTCCGAc                                                                                         <  1:465250/140‑1 (MQ=255)                                    aCGGCAACGACGCCTTCTTTGATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGAACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAg                                                                                      <  1:301959/140‑1 (MQ=255)                                      ggCAACGACGCCTTCTTTGATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGAACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCa                                                                                    <  1:105399/140‑1 (MQ=255)                                      ggCAACGACGCCTTCTTTGATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGAACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCa                                                                                    >  2:836840/1‑140 (MQ=255)                                        cAACGACGCCTTCTTTGATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGAACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATg                                                                                  <  2:329721/140‑1 (MQ=255)                                        cAACGACGCCTTCTTTGATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGAACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATg                                                                                  <  2:476295/140‑1 (MQ=255)                                        cAACGACGCCTTCTTTGATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGAACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATg                                                                                  <  2:723862/140‑1 (MQ=255)                                        cAACGACGCCTTCTTTGATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGAACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATg                                                                                  <  2:730793/140‑1 (MQ=255)                                        cAACGACGCCTTCTTTGATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGAACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATg                                                                                  <  1:944338/140‑1 (MQ=255)                                        cAACGACGCCTTCTTTGATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGAACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATg                                                                                  <  1:298039/140‑1 (MQ=255)                                        cAACGACGCCTTCTTTGATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTACAGGCCGAAAAGGATTTTTTTCAGAACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATg                                                                                  >  1:89758/1‑140 (MQ=255)                                           cgacgCCTTCTTTGATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGAACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCa                                                                               >  1:77712/1‑140 (MQ=255)                                                         aTAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGAACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCa                                                                 <  1:589208/140‑1 (MQ=255)                                                             aCTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGAACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATa                                                             >  2:667611/1‑140 (MQ=255)                                                               tGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGGACCTTTACCAAAATGCAAGTGCCCATCAATCCGACCCGCATGCCAAATAATAACGAACAGATAAt                                                           >  2:382761/1‑140 (MQ=255)                                                               tGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGAACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAAt                                                           >  1:423469/1‑140 (MQ=255)                                                                 cAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGAACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGc                                                         >  1:346943/1‑140 (MQ=255)                                                                 cAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGAACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGc                                                         >  2:408740/1‑140 (MQ=255)                                                                 cAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGACCGAAAAGGATTTTTTTCAGAACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGc                                                         >  1:227513/1‑140 (MQ=255)                                                                    cATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGAACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTcc                                                      >  2:736611/1‑140 (MQ=255)                                                                      tGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGAACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAg                                                    >  2:649862/1‑140 (MQ=255)                                                                         ccTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGAACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGa                                                 >  2:846073/1‑140 (MQ=255)                                                                               gTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGAACCTTAACCAGAATGCAGGTGCCAATCAGTCCGGCCAGCATGCCAAATAATACCGGCCAGATAATGCTCCAGCGACTGTCa                                           <  1:140077/140‑1 (MQ=255)                                                                                   cTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGAACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGa                                       >  1:734422/1‑140 (MQ=255)                                                                                   cTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGAACC‑TAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGAt                                      >  1:189633/1‑140 (MQ=255)                                                                                          aGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGAACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTg                                <  2:429991/140‑1 (MQ=255)                                                                                               ggAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGAACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTca                           <  2:36428/140‑1 (MQ=255)                                                                                                 agaTTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGAACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTcaca                         <  1:408740/140‑1 (MQ=255)                                                                                                 agaTTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGAACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTcaca                         <  1:836840/140‑1 (MQ=255)                                                                                                    tttCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGAACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACgg                      >  2:106814/1‑140 (MQ=255)                                                                                                      tCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGAACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCt                    <  2:346943/140‑1 (MQ=255)                                                                                                      tCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGAACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCt                    <  1:649862/140‑1 (MQ=255)                                                                                                      tCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGAACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCt                    <  2:77712/140‑1 (MQ=255)                                                                                                      tCGTAGGGTTACAGGCCGAAAAGGATTTTTTTCAGAACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCt                    <  2:89758/140‑1 (MQ=255)                                                                                                              ttCCAGGCCGAAAAGGATTTTTTTCAGAACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCaa            >  2:537728/1‑140 (MQ=255)                                                                                                                caaGGCCGAAAAGGATTTTTTTCAGAACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAgg          <  1:382761/138‑1 (MQ=255)                                                                                                                        aaaaGGATTTTTTTCAGAACCTTAACCAGAATGCAGGTGACAATCAGTCAGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGc  >  2:77086/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                           CATCGTTGATCAGCGTGACCTCGCCGCGATCGTCCACGGCAACGACGCCTTCTTTGATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGC  >  NC_000913/4350915‑4351173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑