Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,813,315	A→T	intergenic (‑183/+106)	ydjO ← / ← cedA	protein YdjO/cell division modulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,813,315	0	A	T	100.0%	178.9 / NA	52	intergenic (‑183/+106)	ydjO/cedA	protein YdjO/cell division modulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (26/26);  total (26/26)

AAGTCATTTACAATATAAGTTTAAGAGCGACGCCACA‑GGATGAACTATCAAAAATAGCTCATCATGATTAGCAAAACTTAACCATTTTAAAATAAATAAACAATTAAAGAAAAAAGATCACTTATTTATAGCAATAGATCGTCAAAGGCAGCTTTTTGTTACAGGTGGTTTGAATGAATGTAGCAACGAAATACAGAATTTCAGGTCATGTAACTCCCGGCAAAACCGGGAGGTATGTAATCCTTACTCAGTCACTTCCCCTTCCTGGCGGATC  >  NC_000913/1813178‑1813451                                                                                                                                           |                                                                                                                                         aaGTCATTTACAATATAAGTTTAAGAGCGACGCCACA‑GGATGAACTATCAAAAATAGCTCATCATGATTAGCAAAACTTAACCATTTTAAAATAAATAAACAATTAAAGAAAAAAGATCACTTATTTATAGCAATAGTTc                                                                                                                                        >  2:823747/1‑140 (MQ=255) aaGTCATTTACAATATAAGTTTAAGAGCGACGCCACA‑GGATGAACTATCAAAAATAGCTCATCATGATTAGCAAAACTTAACCATTTTAAAATAAATAAACAATTAAAGAAAAAAGATCACTTATTTATAGCAATAGTTc                                                                                                                                        >  2:561607/1‑140 (MQ=255) aaGTCATTTACAATATAAGTTTAAGAGCGACGCCACA‑GGATGAACTATCAAAAATAGCTCATCATGATTAGCAAAACTTAACCATTTTAAAATAAATAAACAATTAAAGAAAAAAGATCACTTATTTATAGCAATAGTTc                                                                                                                                        >  1:523442/1‑140 (MQ=255)        ttACAATATAAGTTTAAGAGCGACGCCACA‑GGATGAACTATCAAAAATAGCTCATCATGATTAGCAAAACTTAACCATTTTAAAATAAATAAACAATTAAAGAAAAAAGATCACTTATTTATAGCAATAGTTCGTCAAAg                                                                                                                                 <  1:241366/140‑1 (MQ=255)         tACAATATAAGTTTAAGAGCGACGCCACA‑GGATGAACTATCAAAAATAGCTCATCATGATTAGCAAAACTTAACCATTTTAAAATAAATAAACAATTAAAGAAAAAAGATCACTTATTTATAGCAATAGTTCGTCAAAgg                                                                                                                                <  1:804163/140‑1 (MQ=255)              tataAGTTTAAGAGCGACGCCACA‑GGATGAACTATCAAAAATAGCTCATCATGATTAGCAAAACTTAACCATTTTAAAATAAATAAACAATTAAAGAAAAAAGATCACTTATTTATAGCAATAGTTCGTCAATGGCAGCt                                                                                                                           <  2:233806/140‑1 (MQ=255)               ataAGTTTAAGAGCGACGCCACA‑GGATGAACTATCAAAAATAGCTCATCATGATTAGCAAAACTTAACCATTTTAAAATAAATAAACAATTAAAGAAAAAAGATCACTTATTTATAGCAATAGTTCGTCAAAGGCAGCtt                                                                                                                          <  2:794789/140‑1 (MQ=255)                taAGTTTAAGAGCGACGCCACA‑GGATGAACTATCAAAAATAGCTCATCATGATTAGCAAAACTTAACCATTTTAAAATAAATAAACAATTAAAGAAAAAAGATCACTTATTTATAGCAATAGTTCGTCAAAGGCAGCttt                                                                                                                         <  1:750628/140‑1 (MQ=255)                  aGTTTAAGAGCGACGCCACA‑GGATGAACTATCAAAAATAGCTCATCATGATTAGCAAAACTTAACCATTTTAAAATAAATAAACAATTAAAGAAAAAAGATCACTTATTTATAGCAATAGTTCGTCAAAGGCAGCttttt                                                                                                                       >  1:764450/1‑140 (MQ=255)                    tttAAGAGCGACGCCACA‑GGATGAACTATCAAAAATAGCTCATCATGATTAGCAAAACTTAACCATTTTAAAATAAATAAACAATTAAAGAAAAAAGATCACTTATTTATAGCAATAGTTCGTCAAAGGCAGCTTTTTGt                                                                                                                     <  1:379588/140‑1 (MQ=255)                      tAAGAGCGACGCCACA‑GGATGAACTATCAAAAATAGCTCATCATGATTAGCAAAACTTAACCATTTTAAAATAAATAAACAATTAAAGAAAAAAGATCACTTATTTATAGCAATAGTTCGTCAAAGGCAGCTTTTTGTTa                                                                                                                   >  1:635534/1‑140 (MQ=255)                      tAAGAGCGACGCCACA‑GGATGAACTATCAAAAATAGCTCATCATGATTAGCAAAACTTAACCATTTTAAAATAAATAAACAATTAAAGAAAAAAGATCACTTATTTATAGCAATAGTTCGTCAAAGGCAGCTTTTTGTTa                                                                                                                   <  2:100629/140‑1 (MQ=255)                      tAAGAGCCACGCCACAGGGATGAACTATCAAAAATAGCTCATCATGATTAGCAAAACTTAACCATTTTAAAATAAATAAACAATTAAAGAAAAAAGATCACTTATTTATAGCAATAGTTCGTCAAAGGCAGCTTTTTGtt                                                                                                                    >  2:808477/1‑140 (MQ=255)                          agCGACGCCACA‑GGATGAACTATCAAAAATAGCTCATCATGATTAGCAAAACTTAACCATTTTAAAATAAATAAACAATTAAAGAAAAAAGATCACTTATTTATAGCAATAGTTCGTCAAAGGCAGCTTTTTGTTACAgg                                                                                                               <  1:452412/140‑1 (MQ=255)                          agCGACGCCACA‑GGATGAACTATCAAAAATAGCTCATCATGATTAGCAAAACTTAACCATTTTAAAATAAATAAACAATTAAAGAAAAAAGATCACTTATTTATAGCAATAGTTCGTCAAAGGCAGCTTTTTGTTACAgg                                                                                                               <  2:917370/140‑1 (MQ=255)                              aCGCCACA‑GGATGAACTATCAAAAATAGCTCATCATGATTAGCAAAACTTAACCATTTTAAAATAAATAAACAATTAAAGAAAAAAGATCACTTATTTATAGCAATAGTTCGTCAAAGGCAGCTTTTTGTTACAggtggt                                                                                                           <  1:506050/140‑1 (MQ=255)                                  caca‑GGATGAACTATCAAAAATAGCTCATCATGATTAGCAAAACTTAACCATTTTAAAATAAATAAACAATTAAAGAAAAAAGATCACTTATTTATAGCAATAGTTCGTCAAAGGCAGCTTTTTGTTACAGGTGGTTtga                                                                                                       <  1:238151/140‑1 (MQ=255)                                  caca‑GGATGAACTATCAAAAATAGCTCATCATGATTAGCAAAACTTAACCATTTTAAAATAAATAAACAATTAAAGAAAAAAGATCACTTATTTATAGCAATAGTTCGTCAAAGGCAGCTTTTTGTTACAGGTGGTTtga                                                                                                       <  1:44846/140‑1 (MQ=255)                                    ca‑GGATGAACTATCAAAAATAGCTCATCATGATTAGCAAAACTTAACCATTTTAAAATAAATAAACAATTAAAGAAAAAAGATCACTTATTTATAGCAATAGTTCGTCAAAGGCAGCTTTTTGTTACAGGTGGTTtgaat                                                                                                     <  1:808799/140‑1 (MQ=255)                                             aCTATCAAAAATAGCTCATCATGATTAGCAAAACTTAACCATTTTAAAATAAATAAACAATTAAAGAAAAAAGATCACTTATTTATAGCAATAGTTCGTCAAAGGCAGCTTTTTGTTACAGGTGGTTTGAATGAATGTAg                                                                                             >  2:863068/1‑140 (MQ=255)                                               tATCAAAAATAGCTCATCATGATTAGCAAAACTTAACCATTTTAAAATAAATAAACAATTAAAGAAAAAAGATCACTTATTTATAGCAATAGTTCGTCAAAGGCAGCTTTTTGTTACAGGTGGTTTGAATGAATGTAGCa                                                                                           <  1:808477/140‑1 (MQ=255)                                                 tCAAAAATAGCTCATCATGATTAGCAAAACTTAACCATTTTAAAATAAATAAACAATTAAAGAAAAAAGATCACTTATTTATAGCAATAGTTCGTCAAAGGCAGCTTTTTGTTACAGGTGGTTTGAATGAATGTAGCAAc                                                                                         >  2:757296/1‑140 (MQ=255)                                                  cAAAAATAGCTCATCGTGATTAGCAAAACTTAACCATTTTAGAATAAATAAACAATTAAAGAAAAAGGATCACTTATTTATAGCAATAGTTCGTCAAAGGCAGCTTTTTGTTACAGGTGGTTTGAATGAATGTAGCAACg                                                                                        >  2:819561/1‑140 (MQ=255)                                                    aaaaTAGCTCATCATGATTAGCAAAACTTAACCATTTTAAAATAAATAAACAATTAAAGAAAAAAGATCACTTATTTATAGCAATAGTTCGTCAAAGGCAGCTTTTTGTTACAGGTGGTTTGAATGAATGTAGCAACGaa                                                                                      >  1:437393/1‑140 (MQ=255)                                                        tAGCTCATCATGATTAGCAAAACTTAACCATTTTAAAATAAATAAACAATTAAAGAAAAAAGATCACTTATTTATAGCAATAGTTCGTCAAAGGCAGCTTTTTGTTACAGGTGGTTTGAATGAATGTAGCAACGAAATAc                                                                                  >  1:828159/1‑140 (MQ=255)                                                         aGCTCATCATGATTAGCAAAACTTAACCATTTTAAAATAAATAAACAATTAAAGAAAAAAGATCACTTATTTATAGCAATAGTTCGTCAAAGGCAGCTTTTTGTTACAGGTGGTTTGAATGAATGTAGCAACGAAATACa                                                                                 >  2:580047/1‑140 (MQ=255)                                                         aGCTCATCATGATTAGCAAAACTTAACCATTTTAAAATAAATAAACAATTAAAGAAAAAAGATCACTTATTTATAGCAATAGTTCGTCAAAGGCAGCTTTTTGTTACAGGTGGTTTGAATGAATGTAGCAACGAAATACa                                                                                 <  1:823747/140‑1 (MQ=255)                                                           cTCATCATGATTAGCAAAACTTAACCATTTTAAAATAAATAAACAATTAAAGAAAAAAGATCACTTATTTATAGCAATAGTTCGTCAAAGGCAGCTTTTTGTTACAGGTGGTTTGAATGAATGTAGCAACGAAATACAGa                                                                               >  1:165778/1‑140 (MQ=255)                                                                 atGATTAGCAAAACTTAACCATTTTAAAATAAATAAACAATTAAAGAAAAAAGATCACTTATTTATAGCAATAGTTCGTCAAAGGCAGCTTTTTGTTACAGGTGGTTTGAATGAATGTAGCAACGAAATACAGAATTTCa                                                                         <  2:764450/140‑1 (MQ=255)                                                                   gATTAGCAAAACTTAACCATTTTAAAATAAATAAACAATTAAAGAAAAAAGATCACTTATTTATAGCAATAGTTCGTCAAAGGCAGCTTTTTGTTACAGGTGGTTTGAATGAATGTAGCAACGAAATACAGAATTTCAgg                                                                       <  2:437393/140‑1 (MQ=255)                                                                           aaaCTTAACCATTTTAAAATAAATAAACAATTAAAGAAAAAAGATCACTTATTTATAGCAATAGTTCGTCAAAGGCAGCTTTTTGTTACAGGTGGTTTGAATGAATGTAGCAACGAAATACAGAATTTCAGGTCATGTaa                                                               <  1:290264/140‑1 (MQ=255)                                                                                        ttAAAATAAATAAACAATTAAAGAAAAAAGATCACTTATTTATAGCAATAGTTCGTCAAAGGCAGCTTTTTGTTACAGGTGGTTTGAATGAATGTAGCAACGAAATACAGAATTTCAGGTCATGTAACTCCCGGCAAAAc                                                  >  1:505229/1‑140 (MQ=255)                                                                                         tAAAATAAATAAACAATTAAAGAAAAAAGATCACTTATTTATAGCAATAGTTCGTCAAAGGCAGCTTTTTGTTACAGGTGGTTTGAATGAATGTAGCAACGAAATACAGAATTTCAGGTCATGTAACTCCCGGCAAAAcc                                                 <  1:757296/140‑1 (MQ=255)                                                                                            aataaataAACAATTAAAGAAAAAAGATCACTTATTTATAGCAATAGTTCGTCAAAGGCAGCTTTTTGTTACAGGTGGTTTGAATGAATGTAGCAACGAAATACAGAATTTCAGGTCATGTAACTCCCGGCAAAACCggg                                              <  1:561607/140‑1 (MQ=255)                                                                                                 ataaaCAATTAAAGAAAAAAGATCACTTATTTATAGCAATAGTTCGTCAAAGGCAGCTTTTTGTTACAGGTGGTTTGAATGAATGTAGCAACGAAATACAGAATTTCAGGTCATGTAACTCCCGGCAAAACCGGGAGGTa                                         <  2:134140/140‑1 (MQ=255)                                                                                                    aaCAATTAAAGAAAAAAGATCACTTATTTATAGCAATAGTTCGTCAAAGGCAGCTTTTTGTTACAGGTGGTTTGAATGAATGTAGCAACGAAATACAGAATTTCAGGTCATGTAACTCCCGGCAAAACCGGGAGGTATGt                                      <  2:165778/140‑1 (MQ=255)                                                                                                    aaCAATTAAAGAAAAAAGATCACTTATTTATAGCAATAGTTCGTCAAAGGCAGCTTTTTGTTACAGGTGGTTTGAATGAATGTAGCAACGAAATACAGAATTTCAGGTCATGTAACTCCCGGCAAAACCGGGAGGTATGt                                      <  2:523442/140‑1 (MQ=255)                                                                                                         ttAAAGAAAAAAGATCACTTATTTATAGCAATAGTTCGTCAAAGGCAGCTTTTTGTTACAGGTGGTTTGAATGAATGTAGCAACGAAATACAGAATTTCAGGTCATGTAACTCCCGGCAAAACCGGGAGGTATGTAATcc                                 >  1:332394/1‑140 (MQ=255)                                                                                                         ttAAAGAAAAAAGATCACTTATTTATAGCAATAGTTCGTCAAAGGCAGCTTTTTGTTACAGGTGGTTTGAATGAATGTAGCAACGAAATACAGAATTTCAGGTCATGTAACTCCCGGCAAAACCGGGAGGTATGTAATcc                                 >  1:464467/1‑140 (MQ=255)                                                                                                         ttAAAGAAAAAAGATCACTTATTTATAGCAATAGTTCGTCAAAGGCAGCTTTTTGTTACAGGTGGTTTGAATGAATGTAGCAACGAAATACAGAATTTCAGGTCATGTAACTCCCGGCAAAACCGGGAGGTATGTAATcc                                 >  2:562758/1‑140 (MQ=255)                                                                                                         ttAAAGAAAAAAGATCACTTATTTATAGCAATAGTTCGTCAAAGGCAGCTTTTTGTTACAGGTGGTTTGAATGAATGTAGCAACGAAATACAGAATTTCAGGTCATGTAACTCCCGGCAAAACCGGGAGGTATGTAATcc                                 >  2:896703/1‑140 (MQ=255)                                                                                                           aaaGAAAAAAGATCACTTATTTATAGCAATAGTTCGTCAAAGGCAGCTTTTTGTTACAGGTGGTTTGAATGAATGTAGCAACGAAATACAGAATTTCAGGTCATGTAACTCCCGGCAAAACCGGGAGGTATGTAATCCtt                               >  2:662923/1‑140 (MQ=255)                                                                                                                 aaaaGATCACTTATTTATAGCAATAGTTCGTCAAAGGCAGCTTTTTGTTACAGGTGGTTTGAATGAATGTAGCAACGAAATACAGAATTTCAGGTCATGTAACTCCCGGCAAAACCGGGAGGTATGTAATCCTTACTCAg                         >  2:594768/1‑140 (MQ=255)                                                                                                                         aCTTATTTATAGCAATAGTTCGTCAAAGGCAGCTTTTTGTTACAGGTGGTTTGAATGAATGTAGCAACGAAATACAGAATTTCAGGTCATGTAACTCCCGGCAAAACCGGGAGGTATGTAATCCTTACTCAGTCACTTcc                 <  1:611074/140‑1 (MQ=255)                                                                                                                          cTTATTTATAGCAATAGTTCGTCAAAGGCAGCTTTTTGTTACAGGTGGTTTGAATGAATGTAGCAACGAAATACAGAATTTCAGGTCATGTAACTCCCGGCAAAACCGGGAGGTATGTAATCCTTACTCAGTCACTTccc                <  2:635534/140‑1 (MQ=255)                                                                                                                                  taGCAATAGTTCGTCAAAGGCAGCTTTTTGTTACAGGTGGTTTGAATGAATGTAGCAACGAAATACAGAATTTCAGGTCATGTAACTCCCGGCAAAACCGGGAGGTATGTAATCCTTACTCAGTCACTTCCCCTTCCTgg        <  1:594768/140‑1 (MQ=255)                                                                                                                                  taGCAATAGTTCGTCAAAGGCAGCTTTTTGTTACAGGTGGTTTGAATGAATGTAGCAACGAAATACAGAATTTCAGGTCATGTAACTCCCGGCAAAACCGGGAGGTATGTAATCCTTACTCAGTCACTTCCCCTTCCTgg        <  1:819561/140‑1 (MQ=255)                                                                                                                                   aGCAATAGTTCGTCAAAGGCAGCTTTTTGTTACAGGTGGTTTGAATGAATGTAGCAACGAAATACAGAATTTCAGGTCATGTAACTCCCGGCAAAACCGGGAGGTATGTAATCCTTACTCAGTCACTTCCCCTTCCTGGc       >  2:371934/1‑140 (MQ=255)                                                                                                                                     cAATAGTTCGTCAAAGGCAGCTTTTTGTTACAGGTGGTTTGAATGAATGTAGCAACGAAATACAGAATTTCAGGTCATGTAACTCCCGGCAAAACCGGGAGGTATGTAATCCTTACTCAGTCACTTCCCCTTCCTGGCgg     >  1:434464/1‑140 (MQ=255)                                                                                                                                     cAATAGTTCGTCAAAGGCAGCTTTTTGTTACAGGTGGTTTGAATGAATGTAGCAACGAAATACAGAATTTCAGGTCATGTAACTCCCGGCAAAACCGGGAGGTATGTAATCCTTACTCAGTCACTTCCCCTTCCTGGCgg     >  2:279332/1‑140 (MQ=255)                                                                                                                                     cAATAGTTCGTCAAAGGCAGCTTTTTGTTACAGGTGGTTTGAATGAATGTAGCAACGAAATACAGAATTTCAGGTCATGTAACTCCCGGCAAAACCGGGAGGTATGTAATCCTTACTCAGTCACTTCCCCTTCCTGGCgg     >  2:15823/1‑140 (MQ=255)                                                                                                                                     cAATAGTTCGTCAAAGGCAGCTTTTTGTTACAGGTGGTTTGAATGAATGTAGCAACGAAATACAGAATTTCAGGTCATGTAACTCCCGGCAAAACCGGGAGGTATGTAATCCTTACTCAGTCACTTCCCCTTCCTGGCgg     >  1:566151/1‑140 (MQ=255)                                                                                                                                       aTAGTTCGTCAAAGGCAGCTTTTTGTTACAGGTGGTTTGAATGAATGTAGCAACGAAATACAGAATTTCAGGTCATGTAACTCCCGGCAAAACCGGGAGGTATGTAATCCTTACTCAGTCACTTCCCCTTCCTGGCGGAt   >  2:360918/1‑140 (MQ=255)                                                                                                                                        tAGTTCGTCAAAGGCAGCTTTTTGTTACAGGTGGTTTGAATGAATGTAGCAACGAAATACAGAATTTCAGGTCATGTAACTCCCGGCAAAACCGGGAGGTATGTAATCCTTACTCAGTCACTTCCCCTTCCTGGCGGAta  >  2:228210/1‑139 (MQ=255)                                                                                                                                           |                                                                                                                                         AAGTCATTTACAATATAAGTTTAAGAGCGACGCCACA‑GGATGAACTATCAAAAATAGCTCATCATGATTAGCAAAACTTAACCATTTTAAAATAAATAAACAATTAAAGAAAAAAGATCACTTATTTATAGCAATAGATCGTCAAAGGCAGCTTTTTGTTACAGGTGGTTTGAATGAATGTAGCAACGAAATACAGAATTTCAGGTCATGTAACTCCCGGCAAAACCGGGAGGTATGTAATCCTTACTCAGTCACTTCCCCTTCCTGGCGGATC  >  NC_000913/1813178‑1813451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑