New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3617315 =	110 (1.130)	3 (0.030)	3/406	9.3	2.9%	coding (301/765 nt)	nikD	Ni(2(+)) ABC transporter ATP binding subunit NikD
	?	NC_000913	3617414 =	93 (0.990)					coding (400/765 nt)	nikD	Ni(2(+)) ABC transporter ATP binding subunit NikD
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CAGATCGAGGATGCGCGCCTGTGCTACCACGTCAAGGTCGGTGGTCGGTTCATCGGCGATGATAAACGGTGATTCACACAGCACCGCCATCGCAATCATCATGCGCTGCAACATGCCGCCGCTCATCTCGAACGGGTACAGCTTCAGCACGCGCGCGGCGTTTTCCAGCCCCACCGCTTCTATGGCAGCGGTAAGCGTAGCGTCATCGGCGGGTTTCCCTAACGCCAGGCAGGTTTCCCGCGCGTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3617560‑3617315 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgcgcgtgCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCGCCGATGAACCGACCACCGACCTTGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTATGCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGA  >  NC_000913/3617414‑3617640                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           CAGATCGAGGATGCGCGCCTGTGCTACCACGTCAAGGTCGGTGGTCGGTTCATCGGCGATGATAAACGGTGATTCACACAGCACCGCCATCGCAATCATCATGCGCTGCAACATGCCGCCGCTCATCTCGAACGGGTACAGCTTCAGCACGCGCGCGGCGTTTTCCAGCCCCACCGCTTCTATGGCAGCGGTAAGCGTAGCGTCATCGGCGGGTTTCCCTAACGCCAGGCAGGTTTCCC                                                                                                                                                                                                                                            >  1:454643/1‑239    ATCGAGGATGCGCGCCTGTGCTACCACGTCAAGGTCGGTGGTCGGTTCATCGGCGATGATAAACGGTGATTCACACAGCACCGCCATCGCAATCATCATGCGCTGCAACATGCCGCCGCTCATCTCGAACGGGTACAGCTTCAGCACGCGCGCGGCGTTTTCCAGCCCCACCGCTTCTATGGCAGCGGTAAGCGTAGCGTCATCGGCGGGTTTCCCTAACGCCAGGCAGGTTTCCCGCG                                                                                                                                                                                                                                         >  1:600503/1‑239      CGAGGATGCGCGCCTGTGCTACCACGTCAAGGTCGGTGGTCGGTTCATCGGCGATGATAAACGGTGATTCACACAGCACCGCCATCGCAATCATCATGCGCTGCAACATGCCGCCGCTCATCTCGAACGGGTACAGCTTCAGCACGCGCGCGGCGTTTTCCAGCCCCACCGCTTCTATGGCAGCGGTAAGCGTAGCGTCATCGGCGGGTTTCCCTAACGCCAGGCAGGTTTCCCGCGCG                                                                                                                                                                                                                                       <  1:25562/239‑1      CGAGGATGCGCGCCTGTGCTACCACGTCAAGGTCGGTGGTCGGTTCATCGGCGATGATAAACGGTGATTCACACAGCACCGCCATCGCAATCATCATGCGCTGCAACATGCCGCCGCTCATCTCGAACGGGTACAGCTTCAGCACGCGCGCGGCGTTTTCCAGCCCCACCGCTTCTATGGCAGCGGTAAGCGTAGCGTCATCGGCGGGTTTCCCTAACGCCAGGCAGGTTTCCCGCGCG                                                                                                                                                                                                                                       >  1:918690/1‑239      CGAGGATGCGCGCCTGTGCTACCACGTCAAGGTCGGTGGTCGGTTCATCGGCGATGATAAACGGTGATTCACACAGCACCGCCATCGCAATCATCATGCGCTGCAACATGCCGCCGCTCATCTCGAACGGGTACAGCTTCAGCACGCGCGCGGCGTTTTCCAGCCCCACCGCTTCTATGGCAGCGGTAAGCGTAGCGTCATCGGCGGGTTTCCCTAACGCCAGGCAGGTTTCCCGCGCG                                                                                                                                                                                                                                       <  2:610143/239‑1       GAGGATGCGCGCCTGTGCTACCACGTCAAGGTCGGTGGTCGGTTCATCGGCGATGATAAACGGTGATTCACACAGCACCGCCATCGCAATCATCATGCGCTGCAACATGCCGCCGCTCATCTCGAACGGGTACAGCTTCAGCACGCGCGCGGCGTTTTCCAGCCCCACCGCTTCTATGGCAGCGGTAAGCGTAGCGTCATCGGCGGGTTTCCCTAACGCCAGGCAGGTTTCCCGCGCGTG                                                                                                                                                                                                                                     <  2:1059152/240‑1             GCGCGCCTGTGCTACCACGTCAAGGTCGGTGGTCGGTTCATCGGCGATGATAAACGGTGATTCACACAGCACCGCCATCGCAATCATCATGCGCTGCAACATGCCGCCGCTCATCTCGAACGGGTACAGCTTCAGCACGCGCGCGGCGTTTTCCAGCCCCACCGCTTCTATGGCAGCGGTAAGCGTAGCGTCATCGGCGGGTTTCCCTAACGCCAGGCAGGTTTCCCGCGCG                                                                                                                                                                                                                                       <  2:995620/232‑1                                  AAGGTCGGTGGTCGGTTCATCGGCGATGATAAACGGTGATTCACACAGCACCGCCATCGCAATCATCATGCGCTGCAACATGCCGCCGCTCATCTCGAACGGGTACAGCTTCAGCACGCGCGCGGCGTTTTCCAGCCCCACCGCTTCTATGGCAGCGGTAAGCGTAGCGTCATCGGCGGGTTTCCCTAACGCCAGGCAGGTTTCCCGCG                                                                                                                                                                                                                                         <  2:877805/209‑1                                   AGGTCGGTGGTCGGTTCATCGGCGATGATAAACGGTGATTCACACAGCACCGCCATCGCAATCATCATGCGCTGCAACATGCCGCCGCTCATCTCGAACGGGTACAGCTTCAGCACGCGCGCGGCGTTTTCCAGCCCCACCGCTTCTATGGCAGCGGTAAGCGTAGCGTCATCGGCGGGTTTCCCTAACGCCAGGCAGGTTTCCCGCGCGT                                                                                                                                                                                                                                      >  2:1119331/1‑211                                                    ATCGGCGATGATAAACGGTGATTCACACAGCACCGCCATCGCAATCATCATGCGCTGCAACATGCCGCCGCTCATCTCGAACGGGTACAGCTTCAGCACGCGCGCGGCGTTTTCCAGCCCCACCGCTTCTATGGCAGCGGTAAGCGTAGCGTCATCGGCGGGTTTCCCTAACGCCAGGCAGGTTTCCCGCGCG                                                                                                                                                                                                                                       >  2:957764/1‑193                                                                                                                                                                         CCCCACCGCTTCTATGGCAGCGGTAAGCGTAGCGTCATCGGCGGGTTTCCCTAACGCCAGGCAGGTTTCCCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCGCCGATGAACCGACCACCGACCTTGACGTGGTAGCACAGGCGCGCATCCTCGAT                                                                                     >  1:672844/1‑222                                                                                                                                                                         CCCCACCGCTTCTATGGCAGCGGTAAGCGTAGCGTCATCGGCGGGTTTCCCTAACGCCAGGCAGGTTTCCCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCGCCGATGAACCGACCACCGACCTTGACGTGGTAGCACAGGCGCGCATCCTCGAT                                                                                     <  2:672842/222‑1                                                                                                                                                                                                                  CGGGTTTCCCTAACGCCAGGCAGGTTTCCCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCGCCGATGAACCGACCACCGACCTTGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAA                                                                            >  2:400483/1‑190                                                                                                                                                                                                                                                 CGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCGCCGATGAACCGACCACCGACCTTGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTATGCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGA  <  1:805435/233‑1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           CAGATCGAGGATGCGCGCCTGTGCTACCACGTCAAGGTCGGTGGTCGGTTCATCGGCGATGATAAACGGTGATTCACACAGCACCGCCATCGCAATCATCATGCGCTGCAACATGCCGCCGCTCATCTCGAACGGGTACAGCTTCAGCACGCGCGCGGCGTTTTCCAGCCCCACCGCTTCTATGGCAGCGGTAAGCGTAGCGTCATCGGCGGGTTTCCCTAACGCCAGGCAGGTTTCCCGCGCGTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3617560‑3617315 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgcgcgtgCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCGCCGATGAACCGACCACCGACCTTGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTATGCAAAAACAAGCGCCGGGAATGCTGCTGGTGACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGA  >  NC_000913/3617414‑3617640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.