Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,370,387	C→T	G9E (GGG→GAG)	yjeH ←	L‑methionine/branched chain amino acid exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,370,387	0	C	T	100.0%	254.2 / NA	67	G9E (GGG→GAG)	yjeH	L‑methionine/branched chain amino acid exporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/36);  total (31/36)

AAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCAAACACAATCGCAATCGGGAACACTAAGATAATCAAAACGGGCCACGCCCACAGGCTGTTATTGCCCGCTACCAGCGCAGCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCCCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTAGCACAATCAGATTCGCTTATGACGGCGATGAAGAAATTGCGATGAAATGTGAGGTGAATCAGGGTTTTCACCCGATTTTGTGCTGATCAGAATTTTTTTTCTTTTTCCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCACCAGCC  >  NC_000913/4370161‑4370622                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                            aaGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCAAACACAATCGCAATCGGGAACACTAAGATAATCAAAACGGGCCACGCCCACAGGCTGTTATTGCCCGCTACCAGCGCAGCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGttt                                                                                                                                                                                                                                <  1:737918/240‑1 (MQ=255)   gCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCAAACACAATCGCAATCGGGAACACTAAGATAATCAAAACGGGCCACGCCCACAGGCTGTTATTGCCCGCTACCAGCGCAGCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTg                                                                                                                                                                                                                               >  2:376232/1‑239 (MQ=255)     cgcgAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCAAACACAATCGCAATCGGGAACACTAAGATAATCAAAACGGGCCACGCCCACAGGCTGTTATTGCCCGCTACCAGCGCAGCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGt                                                                                                                                                                                                                            <  2:589386/240‑1 (MQ=255)       cgAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCAAACACAATCGCAATCGGGAACACTAAGATAATCAAAACGGGCCACGCCCACAGGCTGTTATTGCCCGCTACCAGCGCAGCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTc                                                                                                                                                                                                                           <  1:715187/239‑1 (MQ=255)       cgAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCAAACACAATCGCAATCGGGAACACTAAGATAATCAAAACGGGCCACGCCCACAGGCTGTTATTGCCCGCTACCAGCGCAGCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTc                                                                                                                                                                                                                           <  1:1063152/239‑1 (MQ=255)       cgAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCAAACACAATCGCAATCGGGAACACTAAGATAATCAAAACGGGCCACGCCCACAGGCTGTTATTGCCCGCTACCAGCGCAGCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTc                                                                                                                                                                                                                           <  2:216256/239‑1 (MQ=255)                  ccATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCAAACACAATCGCAATCGGGAACACTAAGATAATCAAAACGGGCCACGCCCACAGGCTGTTATTGCCCGCTACCAGCGCAGCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGt                                                                                                                                                                                                                <  1:1055753/239‑1 (MQ=255)                  ccATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCAAACACAATCGCAATCGGGAACACTAAGATAATCAAAACGGGCCACGCCCACAGGCTGTTATTGCCCGCTACCAGCGCAGCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGt                                                                                                                                                                                                                <  1:568977/239‑1 (MQ=255)                   cATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCAAACACAATCGCAATCGGGAACACTAAGATAATCAAAACGGGCCACGCCCACAGGCTGTTATTGCCCGCTACCAGCGCAGCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTg                                                                                                                                                                                                              <  2:1025332/240‑1 (MQ=255)                      aCCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCAAACACAATCGCAATCGGGAACACTAAGATAATCAAAACGGGCCACGCCCACAGGCTGTTATTGCCCGCTACCAGCGCAGCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGAt                                                                                                                                                                                                            >  2:734794/1‑239 (MQ=255)                                  cgcgACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCAAACACAATCGCAATCGGGAACACTAAGATAATCAAAACGGGCCACGCCCACAGGCTGTTATTGCCCGCTACCAGCGCAGCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGcc                                                                                                                                                                                               >  2:432154/1‑240 (MQ=255)                                          cgccTGCGCTGGGATAGTGGCGACCCAGAATCGCAAACACAATCGCAATCGGGAACACTAAGATAATCAAAACGGGCCACGCCCACAGGCTGTTATTGCCCGCTACCAGCGCAGCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAAtttt                                                                                                                                                                                       >  2:178520/1‑240 (MQ=255)                                          cgccTGCGCTGGGATAGTGGCGACCCAGAATCGCAAACACAATCGCAATCGGGAACACTAAGATAATCAAAACGGGCCACGCCCACAGGCTGTTATTGCCCGCTACCAGCGCAGCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAAtttt                                                                                                                                                                                       >  1:770393/1‑240 (MQ=255)                                                     ggATAGTGGCGACCCAGAATCGCAAACACAATCGCAATCGGGAACACTAAGATAATCAAAACGGGCCACGCCCACAGGCTGTTATTGCCCGCTACCAGCGCAGCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCg                                                                                                                                                                            <  1:376233/240‑1 (MQ=255)                                                                aCCCAGAATCGCAAACACAATCGCAATCGGGAACACTAAGATAATCAAAACGGGCCACGCCCACAGGCTGTTATTGCCCGCTACCAGCGCAGCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGa                                                                                                                                                                 >  1:738628/1‑240 (MQ=255)                                                                           cAAACACAATCGCAATCGGGAACACTAAGATAATCAAAACGGGCCACGCCCACAGGCTGTTATTGCCCGCTACCAGCGCAGCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTAGCACaa                                                                                                                                                      >  1:217852/1‑240 (MQ=255)                                                                                acaATCGCAATCGGGAACACTAAGATAATCAAAACGGGCCACGCCCACAGGCTGTTATTGCCCGCTACCAGCGCAGCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTa                                                                                                                                                            >  2:740830/1‑229 (MQ=255)                                                                                 caatcgcaatcgGGAACACTAAGATAATCAAAACGGGCCACGCCCACAGGCTGTTATTGCCCGCTACCAGCGCAGCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTAGCACAATCAGa                                                                                                                                                 <  2:671014/239‑1 (MQ=255)                                                                                  aatcgcaatcgGGAACACTAAGATAATCAAAACGGGCCACGCCCACAGGCTGTTATTGCCCGCTACCAGCGCAGCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTAGCACAATCAGAt                                                                                                                                                <  2:332850/239‑1 (MQ=255)                                                                                   atcgcaatcgGGAACACTAAGATAATCAAAACGGGCCACGCCCACAGGCTGTTATTGCCCGCTACCAGCGCAGCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTAGCACAATCAGATTc                                                                                                                                              <  1:734796/240‑1 (MQ=255)                                                                                      gcaatcgGGAACACTAAGATAATCAAAACGGGCCACGCCCACAGGCTGTTATTGCCCGCTACCAGCGCAGCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGt                                                                                                                                                                                                                            <  2:1087813/159‑1 (MQ=255)                                                                                            gggAACACTAAGATAATCAAAACGGGCCACGCCCACAGGCTGTTATTGCCCGCTACCAGCGCAGCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTAGCACAATCAGATTCGCTTATGa                                                                                                                                      <  1:790722/239‑1 (MQ=255)                                                                                               aaCACTAAGATAATCAAAACGGGCCACGCCCACAGGCTGTTATTGCCCGCTACCAGCGCAGCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTAGCACAATCAGATTCGCTTATGACGGc                                                                                                                                  <  1:333815/240‑1 (MQ=255)                                                                                                  acTAAGATAATCAAAACGGGCCACGCCCACAGGCTGTTATTGCCCGCTACCAGCGCAGCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTAGCACAATCAGATTCGCTTATGACGGCGa                                                                                                                                >  2:348591/1‑239 (MQ=255)                                                                                                    tAAGATAATCAAAACGGGCCACGCCCACAGGCTGTTATTGCCCGCTACCAGCGCAGCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTAGCACAATCAGATTCGCTTATGACGGc                                                                                                                                  >  1:12233/1‑235 (MQ=255)                                                                                                             cAAAACGGGCCACGCCCACAGGCTGTTATTGCCCGCTACCAGCGCAGCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTAGCACAATCAGATTCGCTTATGACGGCGATGAAGAAATTg                                                                                                                     <  1:1032525/239‑1 (MQ=255)                                                                                                              aaaaCGGGCCACGCCCACAGGCTGTTATTGCCCGCTACCAGCGCAGCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTAGCACAATCAGATTCGCTTATGACGGCGATGAAGAAATTGCg                                                                                                                   <  1:677870/240‑1 (MQ=255)                                                                                                                aaCGGGCCACGCCCACAGGCTGTTATTGCCCGCTACCAGCGCAGCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTAGCACAATCAGATTCGCTTATGACGGCGATGAAGAAATTGCGa                                                                                                                  >  1:763848/1‑239 (MQ=255)                                                                                                                  cGGGCCACGCCCACAGGCTGTTATTGCCCGCTACCAGCGCAGCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTAGCACAATCAGATTCGCTTATGACGGCGATGAAGAAATTGCGAt                                                                                                                 <  2:533986/238‑1 (MQ=255)                                                                                                                  cGGGCCACGCCCACAGGCTGTTATTGCCCGCTACCAGCGCAGCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTAGCACAATCAGATTCGCTTATGACGGCGATGAAGAAATTGCGATg                                                                                                                <  2:303398/239‑1 (MQ=255)                                                                                                                   gggCCACGCCCACAGGCTGTTATTGCCCGCTACCAGCGCAGCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGTGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATg                                                                                                                                                               <  1:80203/191‑1 (MQ=255)                                                                                                                     gCCACGCCCACAGGCTGTTATTGCCCGCTACCAGCGCAGCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTgc                                                                                                                                                                                                  >  2:874060/1‑154 (MQ=255)                                                                                                                     gCCACGCCCACAGGCTGTTATTGCCCGCTACCAGCGCAGCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTAGCACAATCAGATTCGCTTATGACGGCGATGAAGaaa                                                                                                                        <  1:932831/228‑1 (MQ=255)                                                                                                                     gCCACGCCCACAGGCTGTTATTGCCCGCTACCAGCGCAGCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTAGCACAATCAGATTCGCTTATGACGGCGATGAAGAAATTGCGATGaaa                                                                                                             >  1:709931/1‑239 (MQ=255)                                                                                                                     gCCACGCCCACAGGCTGTTATTGCCCGCTACCAGCGCAGCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTAGCACAATCAGATTCGCTTATGACGGCGATGAAGAAATTGCGATGaaa                                                                                                             >  1:585212/1‑239 (MQ=255)                                                                                                                          gtccACAGGCTGTTATTGCCCGCTACCAGCGCAGCTAACGCAGGAACGGCAAACCCGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCAt                                                                                                                                                                                                                    <  2:863399/129‑1 (MQ=255)                                                                                                                          gCCCACAGGCTGTTATTGCCCGCTACCAGCGCAGCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTAGCACAATCAGATTCGCTTATGACGGCGATGAAGAAATTGCGATGAAATGTGAg                                                                                                       >  2:1055174/1‑240 (MQ=255)                                                                                                                           cccACAGGCTGTTATTGCCCGCTACCAGCGCAGCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTAGCACAATCAGATTCGCTTATGACGGCGATGAAGAAATTGCGATGAAATGTGAgg                                                                                                      <  1:348592/240‑1 (MQ=255)                                                                                                                           cccACAGGCTGTTATTGCCCGCTACCAGCGCAGCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTAGCACAATCAGATTCGCTTATGACGGCGATGAAGAAATTGCGATGAAATGTGAg                                                                                                       <  1:325024/239‑1 (MQ=255)                                                                                                                                       tATTGCCCGCTACCAGCGCAGCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTAGCACAATCAGATTCGCTTATGACGGCGATGAAGAAATTGCGATGAAATGTGAg                                                                                                       <  2:198706/227‑1 (MQ=255)                                                                                                                                            cccGCTACCAGCGCAGCTAACGCAGGAACGGCAAACACGCCAGTGTCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTAGCACAATCAGATTCGCTTATGACGGCGATGAAGAAATTGCGATGAAATGTGAGGTGAATCAGGGTTTTCAc                                                                                     >  1:1069776/1‑240 (MQ=255)                                                                                                                                            cccGCTACCAGCGCAGCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTAGCACAATCAGATTCGCTTATGACGGCGATGAAGAAATTGCGATGAAATGTGAGGTGAATCAGGGTTTTCAc                                                                                     >  1:65988/1‑240 (MQ=255)                                                                                                                                             ccGCTACCAGCGCAGCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTAGCACAATCAGATTCGCTTATGACGGCGATGAAGAAATTGCGATGAAATGTGAGGTGAATCAGGGTTTTCAcc                                                                                    <  1:550709/240‑1 (MQ=255)                                                                                                                                                cTACCAGCGCAGCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTAGCACAATCAGATTCGCTTATGACGGCGATGAAGAAATTGCGATGAAATGTGAGGTGAATCAGGGTTTTCACCCg                                                                                  >  1:702006/1‑239 (MQ=255)                                                                                                                                                     accgcAGCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTa                                                                                                                                                            <  2:833973/158‑1 (MQ=255)                                                                                                                                                     aGCGCAGCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTAGCACAATCAGATTCGCTTATGACGGCGATGAAGAAATTGCGATGAAAtg                                                                                                           <  2:1120835/209‑1 (MQ=255)                                                                                                                                                           gCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCTGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTAGCACAATCAGATTCGCTTATGACGGCGATGAAGAAATTGCGATGAAATGTGAGGTGAATCAgg                                                                                             >  2:26439/1‑217 (MQ=255)                                                                                                                                                           gCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTAGCACAATCAGATTCGCTTATGACGGCGATGAAGAAATTGCGATGAAATGTGAGGTGAATCAGGGTTTTCACCCGATTTTGTGCTg                                                                       >  1:1106388/1‑239 (MQ=255)                                                                                                                                                             tAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATGTTCGa                                                                                                                                                                 <  2:976915/147‑1 (MQ=255)                                                                                                                                                                      aaCGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTAGCACAATCAGATTCGCTTATGACGGCGATGAAGAAATTGCGATGAAATGTGAGGTGAATCAGGGTTTTCACCCGATTTTGTGCTGATCAGAAttttt                                                           >  1:907791/1‑240 (MQ=255)                                                                                                                                                                             aaCACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTAGCACAATCAGATTCGCTTATGACGGCg                                                                                                                                 >  1:910053/1‑163 (MQ=255)                                                                                                                                                                                acGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTAGCACAATCAGATTCGCTTATGACGGCGATGAAGAAATTGCGATGAAATGTGAGGTGAATCAGGGTTTTCACCCGATTTTGTGCTGATCAGAATTTtttttctttttc                                                 <  1:833913/240‑1 (MQ=255)                                                                                                                                                                                         ccTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTAGCACAATCAGATTCGCTTATGACGGCGATGAAGAAATTGCGATGAAATGTGAGGTGAATCAGGGTTTTCACCCGATTTTGTGCTGATCAGAATTTTTTTTCTTTTTCCCCCTTGaa                                        >  1:65523/1‑240 (MQ=255)                                                                                                                                                                                               aatGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTAGCACAATCAGATTCGCTTATGACGGCGATGAAGAAATTGCGATGAAATGTGAGGTGAATCAGGGTTTTCACCCGATTTTGTGCTGATCAGAATTTTTTTTCTTTTTCCCCCTTGAAGGGGCg                                  <  2:230617/240‑1 (MQ=255)                                                                                                                                                                                                   aCGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTAGCACAATCAGATTCGCTTATGACGGCGATGAAGAAATTGCGATGAAATGTGAGGTGAATCAGGGTTTTCACCCGATTTtgtg                                                                          >  2:47673/1‑196 (MQ=255)                                                                                                                                                                                                   aCGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTAGCACAATCAGATTCGCTTATGACGGCGATGAAGAAATTGCGATGAAATGTGAGGTGAATCAGGGTTTTCACCCGATTTTGTGCTGATCAGAATTTTTTTTCTTTTTCCCCCTTGAAGGGGCGaa                                >  1:137698/1‑238 (MQ=255)                                                                                                                                                                                                   aCGTCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGACTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTAGCACAATCAGATTCGCTTATGACGGCGCTGAAGAAATTGCGATGAAATGTGAGGTGAATCAGGGCTTACACCCGAtttt                                                                             >  2:720417/1‑193 (MQ=255)                                                                                                                                                                                                      tCGATAGCAGGCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTAGCACAATCAGATTCGCTTATGACGGCGATGAAGAAATTGCGATGAAATGTGAGGTGAATCAGGGTTTTCACCCGATTTTGTGCTGATCAGAATTTTTTTTCTTTTTCCCCCTTGAAGGGGCGAAGCCTc                           >  2:377784/1‑240 (MQ=255)                                                                                                                                                                                                               ggCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTAGCACAATCAGATTCGCTTATGACGGCGATGAAGAAATTGCGATGAAATGTGAGGTGAATCAGGGTTTTCACCCGATTTTGTGCTGATCAGAATTTTTTTTCTTTTTCCCCCTTGAAGGGGCGAAGCCTCATCCCCAtt                  >  1:859784/1‑240 (MQ=255)                                                                                                                                                                                                               ggCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTAGCACAATCAGATTCGCTTATGACGGCGATGAAGAAATTGCGATGAAATGTGAGGTGAATCAGGGTTTTCACCCGATTTTGTGCTGATCAGAATTTTTTTTCTTTTTCCCCCTTGAAGGGGCGAAGCCTCATCCCCAtt                  >  2:845379/1‑240 (MQ=255)                                                                                                                                                                                                               ggCCAATGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTAGCACAATCAGATTCGCTTATGACGGCGATGAAGAAATTGCGATGAAATGTGAGGTGAATCAGGGTTTTCACCCGATTTTGTGCTGATCAGAATTTTTTTTCTTTTTCCCCCTTGAAGGGGCGAAGCCTCATCCCCAtt                  >  1:1041675/1‑240 (MQ=255)                                                                                                                                                                                                                     tGCCCTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTAGCACAATCAGATTCGCTTATGACGGCGATGAAGAAATTGCGATGAAATGTGAGGTGAATCAGGGTTTTCACCCGATTTTGTGCTGATCAGAATTTTTTTTCTTTTTCCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTctct             >  2:906470/1‑239 (MQ=255)                                                                                                                                                                                                                        ccTGGGCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTAGCACAATCAGATTCGCTTATGACGGCGATGAAGAAATCGCGATGAAATGTGAGGTGAATCAGGGTTTTCACCCGATTTTGTGCTGATCAGAATTTTTTTTCTTTTTCCCCCTTGAAGGGGCGAAGCCt                            <  2:101546/221‑1 (MQ=255)                                                                                                                                                                                                                           gggCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTAGCACAATCAGATTCGCTTATGACGGCGATGAAGAAATTGCGATGAAATGTGAGGTGAATCAGGGTTTTCACCCGATTTTGTGCTGATCAGAATTTTTTTTCTTTTTCCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCAcc      <  2:65988/240‑1 (MQ=255)                                                                                                                                                                                                                           gggCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTAGCACAATCAGATTCGCTTATGACGGCGATGAAGAAATTGCGATGAAATGTGAGGTGAATCAGGGTTTTCACCCGATTTTGTGCTGATCAGAATTTTTTTTCTTTTTCCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCAcc      <  1:377785/240‑1 (MQ=255)                                                                                                                                                                                                                            ggCCAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTAGCACAATCAGATTCGCTTATGACGGCGATGAAGAAATTGCGATGAAATGTGAGGTGAATCAGGGTTTTCACCCGATTTTGTGCTGATCAGAATTTTTTTTCTTTTTCCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCACCa     <  1:74376/240‑1 (MQ=255)                                                                                                                                                                                                                               cAGCTCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTAGCACAATCAGATTCGCTTATGACGGCGATGAAGAAATTGCGATGAAATGTGAGGTGAATCAGGGTTTTCACCCGATTTTGTGCTGATCAGAATTTTTTTTCTTTTTCCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCACCAGcc  <  1:915295/240‑1 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                            AAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCAAACACAATCGCAATCGGGAACACTAAGATAATCAAAACGGGCCACGCCCACAGGCTGTTATTGCCCGCTACCAGCGCAGCTAACGCAGGAACGGCAAACACGCCAGTGCCTAATAATGACGTCGATAGCAGGCCAATGCCCTGGGCCAGCCCCAGTTCTTGTTTGAGTCCACTCATGGGTTGATGTCCGATTGCGCCCAAATTTTGGGCAACTGCGTAGATTTTCGATGGTAGCACAATCAGATTCGCTTATGACGGCGATGAAGAAATTGCGATGAAATGTGAGGTGAATCAGGGTTTTCACCCGATTTTGTGCTGATCAGAATTTTTTTTCTTTTTCCCCCTTGAAGGGGCGAAGCCTCATCCCCATTTCTCTGGTCACCAGCC  >  NC_000913/4370161‑4370622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑