Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,890,139	T→C	T132A (ACG→GCG)	yeaY ←	Slp family lipoprotein YeaY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,890,139	0	T	C	100.0%	292.9 / NA	76	T132A (ACG→GCG)	yeaY	Slp family lipoprotein YeaY
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (45/31);  total (45/31)

TTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGCCTCCTGAC  >  NC_000913/1889927‑1890375                                                                                                                                                                                                                     |                                                                                                                                                                                                                                             ttctttttctttAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCAcc                                                                                                                                                                                                                   <  1:89819/240‑1 (MQ=255)          tttAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTgg                                                                                                                                                                                                          >  2:906030/1‑240 (MQ=255)                            ttCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAg                                                                                                                                                                                        <  2:59597/240‑1 (MQ=255)                             tCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCg                                                                                                                                                                                                                       <  2:175630/208‑1 (MQ=255)                                           ttGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGaaa                                                                                                                                                                           >  2:382344/1‑238 (MQ=255)                                           ttGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATc                                                                                                                                                                         >  1:795156/1‑240 (MQ=255)                                                 tctcGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGAtt                                                                                                                                                                                                            <  1:722902/199‑1 (MQ=255)                                                  ctcGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCggg                                                                                                                                                                  >  1:230416/1‑240 (MQ=255)                                                    cgcgGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTc                                                                                                                                                                                   <  2:648661/221‑1 (MQ=255)                                                    cgcgGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTc                                                                                                                                                                >  1:608475/1‑240 (MQ=255)                                                      cgGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAAt                                                                                                                                                                          <  2:1065926/228‑1 (MQ=255)                                                            cGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGaaaa                                                                                                                                                         <  2:1070705/239‑1 (MQ=255)                                                             gggATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAAcc                                                                                                                                                       >  1:824291/1‑240 (MQ=255)                                                                     aCCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACAt                                                                                                                                               <  1:924358/240‑1 (MQ=255)                                                                         gCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTc                                                                                                                                                   >  1:595671/1‑232 (MQ=255)                                                                         gCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGc                                                                                                                                           >  2:570214/1‑240 (MQ=255)                                                                         gCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGc                                                                                                                                           >  1:719643/1‑240 (MQ=255)                                                                          ccccATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGc                                                                                                                                           <  1:522369/239‑1 (MQ=255)                                                                          ccccATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGc                                                                                                                                           >  2:514728/1‑239 (MQ=255)                                                                              aTCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACAt                                                                                                                                               <  2:870266/231‑1 (MQ=255)                                                                              aTCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATaaa                                                                                                                                      >  1:571479/1‑240 (MQ=255)                                                                               tCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATaaa                                                                                                                                      >  1:1013398/1‑239 (MQ=255)                                                                               tCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATaaa                                                                                                                                      >  2:944800/1‑239 (MQ=255)                                                                               tCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAAt                                                                                                                                     >  1:337224/1‑240 (MQ=255)                                                                               tCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAAt                                                                                                                                     >  1:611744/1‑240 (MQ=255)                                                                               tCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAAt                                                                                                                                     >  1:522691/1‑240 (MQ=255)                                                                                cccccgTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCAcc                                                                                                                                                                     <  2:606554/204‑1 (MQ=255)                                                                                   ccgTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATaaa                                                                                                                                      >  2:66232/1‑235 (MQ=255)                                                                                   ccgTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGa                                                                                                                                 <  1:219271/240‑1 (MQ=255)                                                                                    cgTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGAc                                                                                                                                >  2:407750/1‑240 (MQ=255)                                                                                    cgTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGAc                                                                                                                                >  1:168100/1‑240 (MQ=255)                                                                                    cgTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGAc                                                                                                                                >  1:650593/1‑240 (MQ=255)                                                                                       aGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACAt                                                                                                                                               <  2:719764/222‑1 (MQ=255)                                                                                             aGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACAt                                                                                                                                               <  2:600523/216‑1 (MQ=255)                                                                                             aGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAg                                                                                                                       >  1:917871/1‑240 (MQ=255)                                                                                              gggCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGaa                                                                                                                        <  1:614768/238‑1 (MQ=255)                                                                                               ggCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGt                                                                                                                     <  1:414637/240‑1 (MQ=255)                                                                                               ggCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGt                                                                                                                     <  2:921789/240‑1 (MQ=255)                                                                                                   agccaCGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTtctc                                                                                                                 >  1:516204/1‑240 (MQ=255)                                                                                                   agccaCGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGATGGGTTAAATGACAACGTTTGTAAAGCGTTAATTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGGCGTCAACCGCACCAGTGATTGGcc                                                                                                                                                                                                        >  2:1066665/1‑153 (MQ=255)                                                                                                    gccaCGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTcc                                                                                                                >  2:257473/1‑240 (MQ=255)                                                                                                     ccaCGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTcc                                                                                                                <  1:1100178/239‑1 (MQ=255)                                                                                                         gACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGc                                                                                                            >  1:961924/1‑239 (MQ=255)                                                                                                          aCCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCg                                                                                                           <  2:1069888/239‑1 (MQ=255)                                                                                                          aCCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGt                                                                                                          >  1:1117440/1‑240 (MQ=255)                                                                                                              ccATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCCGaa                                                                                                                                                           >  2:999927/1‑187 (MQ=255)                                                                                                                       cATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCg                                                                                             <  2:1051499/240‑1 (MQ=255)                                                                                                                       cATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCg                                                                                             <  2:919713/240‑1 (MQ=255)                                                                                                                         tGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGc                                                                                            >  2:845421/1‑239 (MQ=255)                                                                                                                         tGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGc                                                                                            >  2:196525/1‑239 (MQ=255)                                                                                                                         tGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGc                                                                                            >  1:307222/1‑239 (MQ=255)                                                                                                                         tGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCt                                                                                           >  1:1032837/1‑240 (MQ=255)                                                                                                                         tGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCt                                                                                           >  1:533661/1‑240 (MQ=255)                                                                                                                            aTCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTc                                                                                        <  1:782508/240‑1 (MQ=255)                                                                                                                                aTCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACCTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGc                                                                                                            >  2:1044939/1‑216 (MQ=255)                                                                                                                                    gCTGAGGGGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGt                                                                                                                     <  1:308645/203‑1 (MQ=255)                                                                                                                                        aGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGcggtacggt                                                                            >  1:72773/1‑240 (MQ=255)                                                                                                                                                       ctgctgGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAg                                                                                                                       >  2:834691/1‑182 (MQ=255)                                                                                                                                                          ctgGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAg                                                           <  2:789581/239‑1 (MQ=255)                                                                                                                                                             ggTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGtttt                                                       >  1:196632/1‑240 (MQ=255)                                                                                                                                                                 aaaTGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCt                                                   >  1:592569/1‑240 (MQ=255)                                                                                                                                                                 aaaTGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTAGCTCCGCTGTCCAGCGGTACGGTAg                                                                          >  2:607168/1‑217 (MQ=255)                                                                                                                                                                     gCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGc                                                <  2:567175/239‑1 (MQ=255)                                                                                                                                                                       cAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCg                                                                                                           >  2:802971/1‑178 (MQ=255)                                                                                                                                                                       cAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGAAGCAATTTCCAGGc                                                              >  2:408945/1‑223 (MQ=255)                                                                                                                                                                                tAACCCGTTACTTGCATCACCATAAATTTATCGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGc                                                <  2:870154/228‑1 (MQ=255)                                                                                                                                                                                   cccGTTACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGa                                                                                                                                                                                                     <  2:737791/76‑1 (MQ=255)                                                                                                                                                                                       ttACTTGCATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAAcc                              >  1:572042/1‑239 (MQ=255)                                                                                                                                                                                              cATCACCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGcc                      >  2:43742/1‑240 (MQ=255)                                                                                                                                                                                                  aCCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGccacca                  >  1:631691/1‑240 (MQ=255)                                                                                                                                                                                                  aCCATAAATTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGccacc                   >  2:744008/1‑239 (MQ=255)                                                                                                                                                                                                        aaTTTATAGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGt            <  2:595670/240‑1 (MQ=255)                                                                                                                                                                                                              taGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGcctcc      >  1:985384/1‑240 (MQ=255)                                                                                                                                                                                                              taGGGCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGcctc       <  2:168100/239‑1 (MQ=255)                                                                                                                                                                                                                gggCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGcctcct     <  1:944802/239‑1 (MQ=255)                                                                                                                                                                                                                gggCGCATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGcctcct     <  2:870637/239‑1 (MQ=255)                                                                                                                                                                                                                  gcgcATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGCCTCCTGAc  <  2:5670/240‑1 (MQ=255)                                                                                                                                                                                                                  gcgcATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGCCTCCTGAc  <  2:1117437/240‑1 (MQ=255)                                                                                                                                                                                                                  gcgcATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTATCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGCCTCCTGAc  <  2:571478/240‑1 (MQ=255)                                                                                                                                                                                                                     |                                                                                                                                                                                                                                             TTCTTTTTCTTTAAAAACAAGCAATTATTCAGTTACAACTGTTTGTACTCTCGCGGGGCCGGGATTATACCAGCCCCATCCGCCGTAGCCATAGGGCCAGCCACGACCGCCATAAAACCATGGATCAATCGGCTGAGGCGGCATAATCACCTGCTGGGTTAAATGCCAACGTTTGTAACCCGTTACTTGCATCACCATAAATTTATAGGGCGTATTGCCGATTTTGCCGTCAACCGCACCAGTGATTGGCCCGACTACCGTAACCAGTTGTCCACGGAAATCCACCGGGTCCAGAAAACCGTTCACATCGGCATAAATGCGACCGCGAGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGCCTCCTGAC  >  NC_000913/1889927‑1890375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑