BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BN000925	39,136	Δ1 bp	coding (379/774 nt)	aphA →	aminoglycoside 3'‑phospho‑transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BN000925	39,136	0	G	.	100.0%	712.7 / NA	154	coding (379/774 nt)	aphA	aminoglycoside 3'‑phospho‑transferase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (75/79); total (75/79)

Only 100 of 154 total aligned reads displayed.

GACGACGTGACTGATGAAATGGTGAGATTGCGTTGGCTGGCGGGGCACATTTCTGTGCCCTCCGTTGTAAGCTTCGTTCGCACGCCCAATCAGGCATGGCTCCTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCCGTTCAACAGTGGACCACGCATGCAGGCTTGCCCGAGCGCGGGA‑‑GTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGCCGAACAGGTTTGGGAGGCGATGCATCGCCTCCTACCGCTCGCGCCGGACCCAGTCGTGACGCACGGCGATTTTTCACTCGATAATCTACTTATCGTCGAAGGTAAGGTAGTCGGCTGCATCGACG  >  BN000925/38902‑39364

    acgTGACTGATGAAAGGGTGAGATTGCGTTGGCTGGCGGGGCACATTTCTGTGCCCTCCGTTGTAAGCTTCGTTCGCACGCCCAATCAGGCATGGCTCCTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACa                                                                                                                                                                                                                                 <  2:6531/237‑1 (MQ=255)

        gACTGATGAAATGGTGAGATTGCGTTGGCTGGCGGGGCACATTTCTGTGCCCTCCGTTGTAAGCTTCGTTCGCACGCCCAATCAGGCATGGCTCCTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGTgacca                                                                                                                                                                                                                          <  2:248305/240‑5 (MQ=255)

         aCTGATGAAATGGTGAGATTGCGTTGGCTGGCGGGGCACATTTCTGTGCCCTCCGTTGTAAGCTTCGTTCGCACGCCCAATCAGGCATGGCTCCTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGTgacca                                                                                                                                                                                                                          <  2:17098/239‑5 (MQ=255)

         aCTGATGAAATGGTAAGATTGCGTTGGCTGGCGGGGCACATTTCTGTGCCCTCCGTTGTAAGCTTCGTTCGCACGCCCAATCAGGCATGGCTCCTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCAc                                                                                                                                                                                                                        <  1:324986/240‑1 (MQ=255)

             atgaAATGGTGAGATTGCGTTGGCTGGCGGGGCACATTTCTGTGCCCTCCGTTGTAAGCTTCGTTCGCACGCCCAATCAGGCATGGCTCCTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCAt                                                                                                                                                                                                                    >  1:66612/1‑240 (MQ=255)

               gaAATGGTGAGATTGCGTTGGCTGGCGGGGCACATTTCTGTGCCCTCCGTTGTAAGCTTCGTTCGCACGCCCAATCAGGCATGGCTCCTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATg                                                                                                                                                                                                                   <  1:218149/239‑1 (MQ=255)

               gaAATGGTGAGATTGCGTTGGCTGGCGGGGCACATTTCTGTGCCCTCCGTTGTAAGCTTCGTTCGCACGCCCAATCAGGCATGGCTCCTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATg                                                                                                                                                                                                                   >  1:326253/1‑239 (MQ=255)

               gaAATGGTGAGATTGCGTTGGCTGGCGGGGCACATTTCTGTGCCCTCCGTTGTAAGCTTCGTTCGCACGCCCAATCAGGCATGGCTCCTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGc                                                                                                                                                                                                                  <  1:235153/240‑1 (MQ=255)

                                   gctggcGGGGCACATTTCTGTGCCCTCCGTTGTAAGCTTCGTTCGCACGCCCAATCAGGCATGGCTCCTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGa                                                                                                                                                                                              <  2:66609/240‑1 (MQ=255)

                                   gctggcGGGGCACATTTCTGTGCCCTCCGTTGTAAGCTTCGTTCGCACGCCCAATCAGGCATGGCTCCTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGa                                                                                                                                                                                              <  1:240246/240‑1 (MQ=255)

                                    ctggcGGGGCACATTTCTGTGCCCTCCGTTGTAAGCTTCGTTCGCACGCCCAATCAGGCATGGCTCCTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGa                                                                                                                                                                                              <  2:293760/239‑1 (MQ=255)

                                          gggCACATTTCTGTGCCCTCCGTTGTAAGCTTCGTTCGCACGCCCAATCAGGCATGGCTCCTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGTgac                                                                                                                                                                                                                            <  2:249500/204‑3 (MQ=255)

                                             cacaTTTCTGTGCCCTCCGTTGTAAGCTTCGTTCGCACGCCCAATCAGGCATGGCTCCTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGA‑‑gcgtatcgag                                                                                                                                                                                  >  2:337746/1‑231 (MQ=255)

                                             cacaTTTCTGTGCCCTCCGTTGTAAGCTTCGTTCGCACGCCCAATCAGGCATGGCTCCTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGA‑‑gcgtatcgag                                                                                                                                                                                  >  2:20628/1‑231 (MQ=255)

                                             cacaTTTCTGTGCCCTCCGTTGTAAGCTTCGTTCGCACGCCCAATCAGGCATGGCTCCTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGA‑‑gcgtatcgag                                                                                                                                                                                  >  1:280531/1‑231 (MQ=255)

                                                  ttCTGTGCCCTCCGTTGTAAGCTTCGTTCGCACGCCCAATCAGGCATGGCTCCTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGTgacca                                                                                                                                                                                                                          <  2:38795/198‑5 (MQ=255)

                                                     tgtgCCCTCCGTTGTAAGCTTCGTTCGCACGCCCAATCAGGCATGGCTCCTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCggggg                                                                                                                                                                             >  1:208993/1‑239 (MQ=255)

                                                     tgtgCCCTCCGTTGTAAGCTTCGTTCGCACGCCCAATCAGGCATGGCTCCTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCggggg                                                                                                                                                                             >  2:188032/1‑239 (MQ=255)

                                                       tgCCCTCCGTTGTAAGCTTCGTTCGCACGCCCAATCAGGCATGGCTCCTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCggggg                                                                                                                                                                             >  1:692/1‑237 (MQ=255)

                                                         cccTCCGTTGTAAGCTTCGTTCGCACGCCCAATCAGGCATGGCTCCTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGgttgtt                                                                                                                                                                        >  1:259356/1‑240 (MQ=255)

                                                          ccTCCGTTGTAAGCTTCGTTCGCACGCCCAATCAGGCATGGCTCCTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGc                                                                                                                                                                                  <  1:85313/229‑1 (MQ=255)

                                                            tCCGTTGTAAGCTTCGTTCGCACGCCCAATCAGGCATGGCTCCTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGc                                                                                                                                                                                                                  >  1:112164/1‑195 (MQ=255)

                                                            tCCGTTGTAAGCTTCGTTCGCACGCCCAATCAGGCATGGCTCCTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCggggg                                                                                                                                                                             >  2:43650/1‑232 (MQ=255)

                                                            tCCGTTGTAAGCTTCGTTCGCACGCCCAATCAGGCATGGCTCCTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGa                                                                                                                                                                      >  1:93387/1‑239 (MQ=255)

                                                            tCCGTTGTAAGCTTCGTTCGCACGCCCAATCAGGCATGGCTCCTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGAt                                                                                                                                                                     >  2:249837/1‑240 (MQ=255)

                                                              cGTTGTAAGCTTCGTTCGCACGCCCAATCAGGCATGGCTCCTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGcc                                                                                                                                                                                                         >  1:150800/1‑202 (MQ=255)

                                                              cGTTGTAAGCTTCGTTCGCACGCCCAATCAGGCATGGCTCCTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCggggg                                                                                                                                                                             >  2:286442/1‑230 (MQ=255)

                                                              cGTTGTAAGCTTCGTTCGCACGCCCAATCAGGCATGGCTCCTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATg                                                                                                                                                                    >  2:161267/1‑239 (MQ=255)

                                                               gTTGTAAGCTTCGTTCGCACGCCCAATCAGGCATGGCTCCTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGa                                                                                                                                                                                              <  2:137001/212‑1 (MQ=255)

                                                                  gTAAGCTTCGTTCGCACGCCCAATCAGGCATGGCTCCTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCg                                                                                                                                                                                 <  2:174601/222‑1 (MQ=255)

                                                                    aaGCTTCGTTCGCACGCCCAATCAGGCATGGCTCCTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGa                                                                                                                                                             <  2:234244/240‑1 (MQ=255)

                                                                    aaGCTTCGTTCGCACGCCCAATCAGGCATGGCTCCTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGa                                                                                                                                                             <  1:14986/240‑1 (MQ=255)

                                                                    aaGCTTCGTTCGCACGCCCAATCAGGCATGGCTCCTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGa                                                                                                                                                             <  1:145903/240‑1 (MQ=255)

                                                                        ttcgttcgCACGCCCAATCAGGCATGGCTCCTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGGTGTTGATGTCGATGACtt                                                                                                                                                          >  1:184741/1‑239 (MQ=255)

                                                                          cgttcgCACGCCCAATCAGGCATGGCTCCTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACt                                                                                                                                                           >  2:115824/1‑236 (MQ=255)

                                                                          cgttcgCACGCCCAATCAGGCATGGCTCCTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCgg                                                                                                                                                       >  1:354815/1‑239 (MQ=255)

                                                                            ttcgCACGCCCAATCAGGCATGGCTCCTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGAt                                                                                                                                                      <  1:258841/239‑1 (MQ=255)

                                                                            ttcgCACGCCCAATCAGGCATGGCTCCTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATa                                                                                                                                                     <  1:132710/240‑1 (MQ=255)

                                                                                cACGCCCAATCAGGCATGGCTCCTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGa                                                                                                                                                                                              <  1:234800/195‑1 (MQ=255)

                                                                                cACGCCCAATCAGGCATGGCTCCTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAgg                                                                                                                                                  <  2:251234/239‑1 (MQ=255)

                                                                                  cGCCCAATCAGGCATGGCTCCTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGa                                                                                                                                                             <  1:206304/226‑1 (MQ=255)

                                                                                    cccAATCAGGCATGGCTCCTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTCTTGTTGATGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATTCTCC‑TTCAACAGT‑GACCACGCATGCAgtcgg                                                                                                                                                                                                            <  1:147299/177‑5 (MQ=255)

                                                                                             gCATGGCTCCTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTgg                                                                                                                                    >  1:217184/1‑240 (MQ=255)

                                                                                                   cTCCTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCggggg                                                                                                                                                                             >  2:214774/1‑193 (MQ=255)

                                                                                                   cTCCTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGcc                                                                                                                              >  1:221950/1‑240 (MQ=255)

                                                                                                      cTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGCCGaa                                                                                                                           <  2:25682/240‑1 (MQ=255)

                                                                                                       tGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGaa                                                                                                                                          >  1:132764/1‑224 (MQ=255)

                                                                                                       tGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGaa                                                                                                                                          <  2:132758/224‑1 (MQ=255)

                                                                                                       tGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGCCGaa                                                                                                                           >  1:252815/1‑239 (MQ=255)

                                                                                                       tGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGCCGaa                                                                                                                           >  1:238363/1‑239 (MQ=255)

                                                                                                             caGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGgttgt                                                                                                                                                                         >  2:112490/1‑187 (MQ=255)

                                                                                                             caGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGGGAAGGGTGGACGGCCGAACAGGtt                                                                                                                     >  2:117385/1‑239 (MQ=255)

                                                                                                             caGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGA‑‑gcgta                                                                                                                                                                                       >  2:313792/1‑167 (MQ=255)

                                                                                                                cAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGCCGa                                                                                                                            >  2:188228/1‑229 (MQ=255)

                                                                                                                cAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGCCGAACAGGTTTggg                                                                                                                 >  1:55628/1‑240 (MQ=255)

                                                                                                                    aCATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGCCGAACAGGTTTGGGAgg                                                                                                              <  1:332226/239‑1 (MQ=255)

                                                                                                                        ggAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGCCTGCATGCGATCCCCGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAgg                                                                                                                                                  >  2:75569/1‑199 (MQ=255)

                                                                                                                        ggAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGGTGTTGATGTCGATGACTTCg                                                                                                                                                        >  1:103370/1‑193 (MQ=255)

                                                                                                                          aagaaCGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGCCGAACAGGTTTGGGAGGCGATGCa                                                                                                       >  1:100975/4‑240 (MQ=255)

                                                                                                                          aaagaCGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGCCGAACAGGTTTGGGAGGCGATGCa                                                                                                       <  2:90036/240‑1 (MQ=255)

                                                                                                                          aaaaaCGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGCCGAACAGGTTTGGGAGGCGATGCa                                                                                                       <  1:47966/240‑1 (MQ=255)

                                                                                                                          aaaaaCGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGCCGAACAGGTTTGGGAGGCGATGCa                                                                                                       <  1:26736/240‑1 (MQ=255)

                                                                                                                             aaCGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGa                                                                                                                                                             >  2:218044/1‑183 (MQ=255)

                                                                                                                             aaCGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGCCGAACAGGTTTGGGAGGCGATGCATCg                                                                                                    >  1:300012/1‑240 (MQ=255)

                                                                                                                                        aaGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGCCGAACAGGTTTGGGAGGCGATGCATCGCCTCCTACCGc                                                                                         <  1:161274/240‑1 (MQ=255)

                                                                                                                                          gTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGCCGAACAGGTTTGGGAGGCGATGCATCGCCTCCTACCGCt                                                                                        <  1:49720/239‑1 (MQ=255)

                                                                                                                                                     ggATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGCCGAACAGGTTTGGGAGGCGATGCATCGCCTCCTACCGCTCGCGCCGGAccc                                                                            >  2:159068/1‑240 (MQ=255)

                                                                                                                                                     ggATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGCCGAACAGGTTTGGGAGGCGATGCATCGCCTCCTACCGCTCGCGCCGGAcc                                                                             <  1:76247/239‑1 (MQ=255)

                                                                                                                                                       aTTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGa                                                                                                                                                             <  2:312538/157‑1 (MQ=255)

                                                                                                                                                       aTTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGGTGTTGATGTCGATGACGTCGATaa                                                                                                                                                    >  2:193767/1‑166 (MQ=255)

                                                                                                                                                         ttCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGCCGaa                                                                                                                           <  2:44681/189‑1 (MQ=255)

                                                                                                                                                               gctcGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGCCGAACAGGTTTGGGAGGCGATGCa                                                                                                       <  1:116238/200‑1 (MQ=255)

                                                                                                                                                               gCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGCCGaa                                                                                                                           <  1:46139/183‑1 (MQ=255)

                                                                                                                                                                             tgttgACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGCCGAACAGGTTTggg                                                                                                                 >  1:344527/1‑179 (MQ=255)

                                                                                                                                                                             tgttgACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGCCGAACAGGTTTggg                                                                                                                 <  2:344516/179‑1 (MQ=255)

                                                                                                                                                                              gttgACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGCCGAACAGGTTTGGGAGGCGATGCATCGCCTCCTACCGCTCGCGCCGGACCCAGTCGTGACGCACGGCGATTTTTCa                                                   <  1:123667/240‑1 (MQ=255)

                                                                                                                                                                                 gACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGCCGAACAGGTTTGGGAGGCGATGCATCGCCTCCTACCGCTCGCGCCGGACCCAGTCGTGACGCACGGCGATTTTTCACt                                                 <  2:104768/239‑1 (MQ=255)

                                                                                                                                                                                 gACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGCCGAACAGGTTTGGGAGGCGATGCATCGCCTCCTACCGCTCGCGCCGGACCCAGTCGTGACGCACGGCGATTTTTCACt                                                 <  1:214864/239‑1 (MQ=255)

                                                                                                                                                                                          gcggcgTTCATGCGCCGACTGCATGAGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAgcg                                                                                                                                              <  2:283248/137‑1 (MQ=255)

                                                                                                                                                                                                 tCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GCCCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGCCGAACAGGTTTGGGAGGCGATGCATCGCCTCCTACCGCTCGCGCCGGACCCAGTCGTGACGCACGGCGATTTTTCACt                                                 <  2:8007/223‑1 (MQ=255)

                                                                                                                                                                                                   aTGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGCCGAACAGGTTTGGGAGGCGATGCATCGCCTCCTACCGCTCGCGCCGGACCCAGTCGTGACGCACGGCGATTTTTCACTCGATAATCTACTTAtcgtc                              <  1:22592/240‑1 (MQ=255)

                                                                                                                                                                                                   aTGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGCCGAACAGGTTTGGGAGGCGATGCATCGCCTCCTACCGCTCGCGCCGGACCCAGTCGTGACGCACGGCGATTTTTCACTCGATAATCTACTTAtcgtc                              <  1:11845/240‑1 (MQ=255)

                                                                                                                                                                                                    tGCGCCGCCTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGCCGAACAGGTTTGGGAGGCGATGCATCGCCTCCTACCGCTCGCGCCGGACCCAGTCGTGACGCACGGCGATTTTTCACTCGATAATCTACTTAtcgtc                              <  2:578/239‑1 (MQ=255)

                                                                                                                                                                                                     gcgcCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGCCGAACAGGTTTGGGAGGCGATGCATCGCCTCCTACCGCTCGCGCCGGACCCAGTCGTGACGCACGGCGATTTTTCACTCGATAATCTACTTAtcgtcg                             <  1:284116/239‑1 (MQ=255)

                                                                                                                                                                                                      cgcCGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGCCGAACAGGTTTGGGAGGCGATGCATCGCCTCCTACCGCTCGCGCCGGACCCAGTCGTGACGCACGGCGATTTTTCACTCGATAATCTACTTATCGTCGa                            <  1:114938/239‑1 (MQ=255)

                                                                                                                                                                                                        ccGACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGCCGAACAGGt                                                                                                                      >  1:346184/1‑147 (MQ=255)

                                                                                                                                                                                                          gACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGCCGAACAGGTTTGGGAGGCGATGCATCGCCTCCTACCGCTCGCGCCGGACCCAGTCGTGACGCACGGCGATTTTTCACTCGATAATCTACTTATCGTCGaaggta                       <  1:252612/240‑1 (MQ=255)

                                                                                                                                                                                                          gACTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGCCGAACAGGTTTGGGAGGCGATGCATCGCCTCCTACCGCTCGCGCCGGACCCAGTCGTGACGCACGGCGATTTTTCACTCGATAATCTACTTATCGTCGaaggt                        >  1:18538/1‑239 (MQ=255)

                                                                                                                                                                                                           aCTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGCCGAACAGGTTTGGGAGGCGATGCATCGCCTCCTACCGCTCGCGCCGGACCCAGTCGTGACGCACGGCGATTTTTCACTCGATAATCTACTTAt                                  >  2:223585/1‑228 (MQ=255)

                                                                                                                                                                                                           aCTGCATGCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGCCGAACAGGTTTGGGAGGCGATGCATCGCCTCCTACCGCTCGCGCCGGACCCAGTCGTGACGCACGGCGATTTTTCACTCGATAATCTACTTATCGTCGaaggta                       <  1:122359/239‑1 (MQ=255)

                                                                                                                                                                                                                  gCGATCCCAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGCCGAACAGGTTTGGGAGGCGATGCATCGCCTCCTACCGCTCGCGCCGGACCCAGTCGTGACGCACGGCGATTTTTCACTCGATAATCTACTTATCGTCGAAGGTAAGGTAGt                >  1:174653/1‑239 (MQ=255)

                                                                                                                                                                                                                       cccAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGCCGAACAGGTTTGGGAGGCGATGCATCGCCTCCTACCGCTCGCGCCGGACCCAGTCGTGACGCACGGCGATTTTTCACTCGATAATCTACTTATCGTCGAAGGTAAGGTAGTCGGCTg          >  1:26867/1‑240 (MQ=255)

                                                                                                                                                                                                                         cAGTGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGCCGAACAGGTTTGGGAGGCGATGCATCGCCTCCTACCGCTCGCGCCGGACCCAGTCGTGACGCACGGCGATTTTTCACTCGATAATCTACTTATCGTCGAAGGTAAGGTAGTCGGCTGc         >  1:355005/1‑239 (MQ=255)

                                                                                                                                                                                                                            tGAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGCCGAACAGGTTTGGGAGGCGATGCAt                                                                                                      >  1:32744/1‑143 (MQ=255)

                                                                                                                                                                                                                             gAGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGCCGAACAGGTTTGGGAGGCGATGCATCGCCTCCTACCGCTCGCGCCGGACCCAGTCGTGACGCACGGCGATTTTTCACTCGATAATCTACTTATCGTCGAAGGTAAGGTAGTCGGCTGCATCg     >  2:124963/1‑239 (MQ=255)

                                                                                                                                                                                                                              aGCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGCCGAACAGGTTTGGGAGGCGATGCATCGCCTCCTACCGCTCGCGCCGGACCCAGTCGTGACGCACGGCGATTTTTCACTCGATAATCTACTTATCGTCGAAGGTAAGGTAGTCGGCTGCAt       >  2:288538/1‑236 (MQ=255)

                                                                                                                                                                                                                               gCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGCCGAACAGGTTTGGGAGGCGATGCATCGCCTCCTACCGCTCGCGCCGGACCCAGTCGTGACGCACGGCGATTTTTCACTCGATAATCTACTTAtcgtc                              <  2:179513/212‑1 (MQ=255)

                                                                                                                                                                                                                               gCGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGCCGAACAGGTTTGGGAGGCGATGCATCGCCTCCTACCGCTCGCGCCGGACCCAGTCGTGACGCACGGCGATTTTTCACTCGATAATCTACTTATCGTCGAAGGTAAGGTAGTCGGCTGCATCGACg  <  2:326829/240‑1 (MQ=255)

                                                                                                                                                                                                                                cGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGCCGAACAGGTTTGGGAGGCGATGCATCGCCTCCTACCGCTCGCGCCGGACCCAGTCGTGACGCACGGCGATTTTTCACTCGATAATCTACTTATCGTCGAAGGTAAGGTAGTCGGCTGCATCGACg  >  1:55912/1‑239 (MQ=255)

                                                                                                                                                                                                                                cGAATGCTCC‑TTCAACAGT‑GACCACGCATGCAGGCTTGCCCGAGCGCGGGAGCGTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGCCGAACAGGTTTGGGAGGCGATGCATCGCCTCCTACCGCTCGCGCCGGACCCAGTCGTGACGCACGGCGATTTTTCACTCGATAATCTACTTATCGTCGAAGGTAAGGTAGTCGGATGCATCGAc   >  1:152883/1‑238 (MQ=255)

GACGACGTGACTGATGAAATGGTGAGATTGCGTTGGCTGGCGGGGCACATTTCTGTGCCCTCCGTTGTAAGCTTCGTTCGCACGCCCAATCAGGCATGGCTCCTGACAACAGCAATACATGGAAAAACGGCATATCAAGTGCTGAAATCGGATTTCGGAGCCCGTCTCGTTGTTGTTGACGCATTGGCGGCGTTCATGCGCCGACTGCATGCGATCCCAGTGAGCGAATGCTCCGTTCAACAGTGGACCACGCATGCAGGCTTGCCCGAGCGCGGGA‑‑GTATCGAGGCGGGGGTTGTTGATGTCGATGACTTCGATAAGGAGCGCGAAGGGTGGACGGCCGAACAGGTTTGGGAGGCGATGCATCGCCTCCTACCGCTCGCGCCGGACCCAGTCGTGACGCACGGCGATTTTTCACTCGATAATCTACTTATCGTCGAAGGTAAGGTAGTCGGCTGCATCGACG  >  BN000925/38902‑39364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`