BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BN000925	9,802	G→A	D801N (GAC→AAC)	tnpA →	Tn1 transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BN000925	9,802	0	G	A	99.8%	1859.7 / ‑6.0	454	D801N (GAC→AAC)	tnpA	Tn1 transposase
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (227/226); minor base C (0/1); total (227/227)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 454 total aligned reads displayed.

CCGATCAGTATTCCGGCTTTCATGGCATCGTTATACCGGGGACGCTGAGGGACTCTATCTTTGTGCTGGAAGGCCTTCTGGAACAGGAGACCGGGCTGAATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGACCATGATGCCGACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACAGGCTTCAGTGCTGGTCCGTTCATTGCTGAAAAGTGAACGTCCTTCCGGACTGACTCAGGCAATCATTGAAGTGGGGCGCATCAACAAAACGCTGTATCTGCTTAATTATATTGATGATG  >  BN000925/9566‑10033

       gTATTCCGGCTTTCATGGCATCGTTATACCGGGGACGCTGAGGGACTCTATCTTTGTGCTGGAAGGCCTTCTGGAACAGGAGACCGGGCTGAATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCg                                                                                                                                                                                                                                <  2:742776/239‑1 (MQ=255)

       gTATTCCGGCTTTCATGGCATCGTTATACCGGGGACGCTGAGGGACTCTATCTTTGTGCTGGAAGGCCTTCTGGAACAGGAGACCGGGCTGAATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCg                                                                                                                                                                                                                                <  1:395498/239‑1 (MQ=255)

       gTATTCCGGCTTTCATGGCATCGCTATACCGGGGACGCTGAGGGACTCTATCTTTGTGCTGGAAGGCCTTCTGGAACAGGAGACCGGGCTGAATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCg                                                                                                                                                                                                                                <  2:682875/239‑1 (MQ=255)

        tATTCCGGCTTTCATGGCATCGTTATACCGGGGACGCTGAGGGACTCTATCTTTGTGCTGGAAGGCCTTCTGGAACAGGAGACCGGGCTGAATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTg                                                                                                                                                                                                                              <  2:243774/240‑1 (MQ=255)

                 tttCATGGCATCGTTATACCGGGGACGCTGAGGGACTCTATCTTTGTGCTGGAAGGCCTTCTGGAACAGGAGACCGGGCTGAATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGat                                                                                                                                                                                                                     <  1:280864/240‑1 (MQ=255)

                  ttCATGGCATCGTTATACCGGGGACGCTGAGGGACTCTATCTTTGTGCTGGAAGGCCTTCTGGAACAGGAGACCGGGCTGAATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTg                                                                                                                                                                                                                           >  2:717714/1‑233 (MQ=255)

                  ttCATGGCATCGTTATACCGGGGACGCTGAGGGACTCTATCTTTGTGCTGGAAGGCCTTCTGGAACAGGAGACCGGGCTGAATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGat                                                                                                                                                                                                                     >  2:304054/1‑239 (MQ=255)

                  ttCATGGCATCGTTATACCGGGGACGCTGAGGGACTCTATCTTTGTGCTGGAAGGCCTTCTGGAACAGGAGACCGGGCTGAATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGat                                                                                                                                                                                                                     >  1:511654/1‑239 (MQ=255)

                  ttCATGGCATCGTTATACCGGGGACGCTGAGGGACTCTATCTTTGTGCTGGAAGGCCTTCTGGAACAGGAGACCGGGCTGAATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGat                                                                                                                                                                                                                     >  1:297480/1‑239 (MQ=255)

                  ttCATGGCATCGTTATACCGGGGACGCTGAGGGACTCTATCTTTGTGCTGGAAGGCCTTCTGGAACAGGAGACCGGGCTGAATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTACGGCGTGCTGAATGata                                                                                                                                                                                                                    <  2:483812/240‑1 (MQ=255)

                            cGTTATACCGGGGACGCTGAGGGACTCTATCTTTGTGCTGGAAGGCCTTCTGGAACAGGAGACCGGGCTGAATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAgg                                                                                                                                                                                                          >  1:169406/1‑240 (MQ=255)

                             gTTATACCGGGGACGCTGAGGGACTCTATCTTTGTGCTGGAAGGCCTTCTGGAACAGGAGACCGGGCTGAATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAggg                                                                                                                                                                                                         >  2:282297/1‑240 (MQ=255)

                              ttATACCGGGGACGCTGAGGGACTCTATCTTTGTGCTGGAAGGCCTTCTGGAACAGGAGACCGGGCTGAATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAggg                                                                                                                                                                                                         >  1:254805/1‑239 (MQ=255)

                                      gggACGCTGAGGGACTCTATCTTTGTGCTGGAAGGCCTTCTGGAACAGGAGACCGGGCTGAATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGc                                                                                                                                                                                                                             >  2:425933/1‑211 (MQ=255)

                                      gggACGCTGAGGGACTCTATCTTTGTGCTGGAAGGCCTTCTGGAACAGGAGACCGGGCTGAATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAg                                                                                                                                                                                                  >  1:230169/1‑238 (MQ=255)

                                      gggACGCTGAGGGACTCTATCTTTGTGCTGGAAGGCCTTCTGGAACAGGAGACCGGGCTGAATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGAt                                                                                                                                                                                                >  1:708461/1‑240 (MQ=255)

                                      gggACGCTGAGGGACTCTATCTTTGTGCTGGAAGGCCTTCTGGAACAGGAGACCGGGCTGAATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGAt                                                                                                                                                                                                >  1:124992/1‑240 (MQ=255)

                                      gggACGCTGAGGGACTCCATCTTTGTGCTGGAAGGCCTTCTGGAACAGGAGACCGGGCTGAATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGAt                                                                                                                                                                                                >  2:497883/1‑240 (MQ=255)

                                       ggACGCTGAGGGACTCTATCTTTGTGCTGGAAGGCCTTCTGGAACAGGAGACCGGGCTGAATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATc                                                                                                                                                                                               >  2:143102/1‑240 (MQ=255)

                                       ggACGCTGAGGGACTCTATCTTTGTGCTGGAAGGCCTTCTGGAACAGGAGACCGGGCTGAATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATc                                                                                                                                                                                               >  2:113089/1‑240 (MQ=255)

                                        gACGCTGAGGGACTCTATCTTTGTGCTGGAAGGCCTTCTGGAACAGGAGACCGGGCTGAATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCCCGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGATTGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATcc                                                                                                                                                                                              >  1:577516/1‑240 (MQ=255)

                                        gACGCTGAGGGACTCTATCTTTGTGCTGGAAGGCCTTCTGGAACAGGAGACCGGGCTGAATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCa                                                                                                                                                                                                              >  1:518273/1‑224 (MQ=255)

                                        gACGCTGAGGGACTCTATCTTTGTGCTGGAAGGCCTTCTGGAACAGGAGACCGGGCTGAATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATcc                                                                                                                                                                                              >  2:732158/1‑240 (MQ=255)

                                         aCGCTGAGGGACTCTATCTTTGTGCTGGAAGGCCTTCTGGAACAGGAGACCGGGCTGAATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATcc                                                                                                                                                                                              <  2:649795/239‑1 (MQ=255)

                                           gCTGAGGGACTCTATCTTTGTGCTGGAAGGCCTTCTGGAACAGGAGACCGGGCTGAATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATcccc                                                                                                                                                                                            >  1:279738/1‑239 (MQ=255)

                                            cTGAGGGACTCTATCTTTGTGCTGGAAGGCCTTCTGGAACAGGAGACCGGGCTGAATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGt                                                                                                                                                                                                                               >  2:23914/1‑203 (MQ=255)

                                                     tctATCTTTGTGCTGGAAGGCCTTCTGGAACAGGAGACCGGGCTGAATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTc                                                                                                                                                                                 >  2:21763/1‑240 (MQ=255)

                                                               tgCTGGAAGGCCTTCTGGAACAGGAGACCGGGCTGAATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGcc                                                                                                                                                                                                               >  2:370427/1‑200 (MQ=255)

                                                               tgCTGGAAGGCCTTCTGGAACAGGAGACCGGGCTGAATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTggg                                                                                                                                                                       >  1:198826/1‑240 (MQ=255)

                                                               tgCTGGAAGGCCTTCTGGAACAGGAGACCGGGCTGAATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTggg                                                                                                                                                                       >  1:605194/1‑240 (MQ=255)

                                                               tgCTGGAAGGCCTTCTGGAACAGGAGACCGGGCTGAATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTggg                                                                                                                                                                       >  1:537820/1‑240 (MQ=255)

                                                               tgCTGGAAGGCCTTCTGGAACAGGAGACCGGGCTGAATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTggg                                                                                                                                                                       >  2:182532/1‑240 (MQ=255)

                                                                gCTGGAAGGCCTTCTGGAACAGGAGACCGGGCTGAATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGa                                                                                                                                                                      <  1:733638/240‑1 (MQ=255)

                                                                 cTGGAAGGCCTTCTGGAACAGGAGACCGGGCTGAATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTCTGGCGTGCTGAATGat                                                                                                                                                                                                                     >  2:403956/1‑192 (MQ=255)

                                                                 cTGGAAGGCCTTCTGGAACAGGAGACCGGGCTGAATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGa                                                                                                                                                                      >  2:47015/1‑239 (MQ=255)

                                                                 cTGGAAGGCCTTCTGGAACAGGAGACCGGGCTGAATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTG‑CAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACg                                                                                                                                                                    >  1:77864/1‑240 (MQ=255)

                                                                         ccTTCTGGAACAGGAGACCGGGCTGTATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGTCTATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCTGAAAAATAGTCCTTCAGTGGGACGAAATGAt                                                                                                                                                             <  1:351074/240‑1 (MQ=255)

                                                                         ccTTCTGGAACAGGAGACCGGGCTGAATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGCGGGCAATCAg                                                                                                                                                                                                  >  2:353147/1‑203 (MQ=255)

                                                                            tCTGGAACAGGAGACCGGGCTGAATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATcc                                                                                                                                                           >  2:314313/1‑239 (MQ=255)

                                                                            tCTGGAACAGGAGACCGGGCTGAATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCg                                                                                                                                                          >  2:161700/1‑240 (MQ=255)

                                                                                     ggAGACCGGGCTGAATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCt                                                                                                                                                   >  1:629158/1‑238 (MQ=255)

                                                                                     ggAGACCGGGCTGAATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTgg                                                                                                                                                 <  1:451135/240‑1 (MQ=255)

                                                                                     ggAGACCGGGCTGAATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTgg                                                                                                                                                 <  1:687964/240‑1 (MQ=255)

                                                                                      gagaCCGGGCTGAATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGc                                                                                                                                                    >  2:817730/1‑236 (MQ=255)

                                                                                      gagaCCGGGCTGAATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTgg                                                                                                                                                 >  1:162048/1‑239 (MQ=255)

                                                                                      gagaCCGGGCTGAATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAATGGGACGAAATGATc                                                                                                                                                            >  2:435470/1‑228 (MQ=255)

                                                                                                        aCCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCaaa                                                                                                                              >  2:833296/1‑240 (MQ=255)

                                                                                                        aCCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCaaa                                                                                                                              >  2:52465/1‑240 (MQ=255)

                                                                                                            aaaTTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTa                                                                                                                           >  1:476734/1‑239 (MQ=255)

                                                                                                             aaTTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACa                                                                                                                         <  1:182590/240‑1 (MQ=255)

                                                                                                             aaTTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACa                                                                                                                         <  1:618725/240‑1 (MQ=255)

                                                                                                             aaTTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACa                                                                                                                         <  2:552747/240‑1 (MQ=255)

                                                                                                             aaTTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACa                                                                                                                         <  2:569135/240‑1 (MQ=255)

                                                                                                             aaTGATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACa                                                                                                                         <  1:833786/240‑1 (MQ=255)

                                                                                                                  tGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACAGGCtt                                                                                                                    <  2:473960/240‑1 (MQ=255)

                                                                                                                  tGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACAGGCtt                                                                                                                    <  1:306970/240‑1 (MQ=255)

                                                                                                                   gACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACAGGCtt                                                                                                                    <  2:602714/239‑1 (MQ=255)

                                                                                                                       gATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACAGGCTTCAGTg                                                                                                               <  1:95612/240‑1 (MQ=255)

                                                                                                                                 gTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACAg                                                                                                                        >  1:84457/1‑221 (MQ=255)

                                                                                                                                  tACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACAGGCTTCAGTGCTGGTCCGtt                                                                                                     <  1:821726/239‑1 (MQ=255)

                                                                                                                                   aCCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACAGGCTTCAGTGCTGGTCCGTTc                                                                                                    >  1:550807/1‑239 (MQ=255)

                                                                                                                                   aCCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACAGGCTTCAGTGCTGGTCCGTTc                                                                                                    >  2:464521/1‑239 (MQ=255)

                                                                                                                                   aCCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACAGGCTTCAGTGCTGGTCCGTTc                                                                                                    >  1:674878/1‑239 (MQ=255)

                                                                                                                                   aCCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACAGGCTTCAGTGCTGGTCCGTTc                                                                                                    >  2:774347/1‑239 (MQ=255)

                                                                                                                                   aCCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACAGGCTTCAGTGCTGGTCCGTTCa                                                                                                   <  1:241555/240‑1 (MQ=255)

                                                                                                                                   aCCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACAGGCTTCAGTGCTGGTCCGTTCa                                                                                                   <  1:341795/240‑1 (MQ=255)

                                                                                                                                   aCCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACAGGCTTCAGTGCTAGTCCGTTc                                                                                                    >  1:365871/1‑239 (MQ=255)

                                                                                                                                   aCCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCCCCCTGAAACTGGGCAAAGt                                                                                                                            >  2:209103/1‑215 (MQ=255)

                                                                                                                                     cAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACa                                                                                                                         <  2:759929/216‑1 (MQ=255)

                                                                                                                                      aGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACAGGCTTc                                                                                                                   >  1:764947/1‑221 (MQ=255)

                                                                                                                                        cGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACAGGCTTCAGTGCTGGTCCGTTCATTGc                                                                                               >  1:100670/1‑239 (MQ=255)

                                                                                                                                            cTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATc                                                                                                                                                            >  2:791944/1‑174 (MQ=255)

                                                                                                                                                     ggCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACAGGCTTCAGTGCTGGTCCGTTCATTGCTGAAAAGTGAACg                                                                                  <  1:24477/239‑1 (MQ=255)

                                                                                                                                                     ggCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACAGGCTTCAGTGCTGGTCCGTTCATTGCTGAAAAGTGAACg                                                                                  <  1:857015/239‑1 (MQ=255)

                                                                                                                                                     ggCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACAGGCTTCAGTGCTGGTCCGTTCATTGCTGAAAAGTGAACg                                                                                  <  1:430810/239‑1 (MQ=255)

                                                                                                                                                     ggCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACAGGCTTCAGTGCTGGTCCGTTCATTGCTGAAAAGTGAACg                                                                                  <  2:802267/239‑1 (MQ=255)

                                                                                                                                                          tttCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACAGGCTTCAGTGCTGGTCCGTTCATTGCTGAAAAGTGAACGTCCtt                                                                             >  1:144282/1‑239 (MQ=255)

                                                                                                                                                               ggCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACAGGCTTCAGTGCTGGTCCGTTCATTGCTGAAAAGTGAACGTCCTTCCGga                                                                        <  1:647724/239‑1 (MQ=255)

                                                                                                                                                                         aTACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACAGGCTTCAGTGCTGGTCCGTTCATTGCTGAAAAGTGAACGTCCTTCCGGACTGACTCAGGc                                                             <  1:215646/240‑1 (MQ=255)

                                                                                                                                                                          tACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACAGGCTTCAGTGCTGGTCCGTTCATTGCTGAAAAGTGAACGTCCTTCCGGACTGACTCAGGc                                                             >  1:380611/1‑239 (MQ=255)

                                                                                                                                                                                  ttCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACAGGCTTCAGTGCTGGTCCGTTCATTGCTGAAAAGTGAACGTCCTTCCGgact                                                                      >  1:717731/1‑222 (MQ=255)

                                                                                                                                                                                         cGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACAGGCTTCAGTGCTGGTCCGTTCATTGCTGAAAAGTGAACGTCCTTCCGGACTGACTCAGGCAATCATTGAAGTgggg                                             <  1:610019/240‑1 (MQ=255)

                                                                                                                                                                                         cGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACAGGCTTCAGTGCTGGTCCGTTCATTGCTGAAAAGTGAACGTCCTTCCGGACTGACTCAGGCAATCATTGAAGTgggg                                             <  1:65676/240‑1 (MQ=255)

                                                                                                                                                                                          gCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACAGGCTTCAGTGCTGGTCCGTTCATTGCTGAAAAGTGAACGTCCTTCCGGACTGACTCAGGCAATCATTGAAGTgggg                                             <  1:493627/239‑1 (MQ=255)

                                                                                                                                                                                                cTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACAGGCTTCAGTGCTGGTCCGTTCATTGCTGAAAAGTGAACGTCCTTCCGGACTGACTCAGGCAATCATTGAAGTgggg                                             <  2:594977/233‑1 (MQ=255)

                                                                                                                                                                                                   aTGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACAGGCTTCAGTGCTGGTCCGTTCATTGCTGAAAAGTGAACGTCCTTCCGGACTGACTCAGGCAATCATTGAAGTGGGGCGCATcaaca                                   >  1:790277/1‑240 (MQ=255)

                                                                                                                                                                                                    tGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACAGGCTTCAGTGCTGGTCCGTTCATTGCTGAAAAGTGAACGTc                                                                                >  2:338260/1‑194 (MQ=255)

                                                                                                                                                                                                    tGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACAGGCTTCAGTGCTGGTCCGTTCATTGCTGAAAAGTGAACGTCCTTCCGGACTGACTCAGGCAATCATTGAAGTGGGGCGCATcaacaa                                  >  2:256365/1‑240 (MQ=255)

                                                                                                                                                                                                    tGCCGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACAGGCTTCAGTGCTGGTCCGTTCATTGCTGAAAAGTGAACGTCCTTCCGGACTGACTCAGGCAATCATTGAAGTGGGGCGCATcaacaa                                  >  1:418937/1‑240 (MQ=255)

                                                                                                                                                                                                       cNGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACAGGCtt                                                                                                                    <  2:90222/155‑1 (MQ=255)

                                                                                                                                                                                                       cGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACAGGCTTCAGTg                                                                                                               <  2:295004/160‑1 (MQ=255)

                                                                                                                                                                                                       cGGTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACAGGCTTCAGTGCTGGTCCGTTCATTGCTGAAAAGTGAACGTCCTTCCGGACTGACTCAGGCAATCATTGAAGTGGGGCGCATCAACAAAAc                               <  1:109533/240‑1 (MQ=255)

                                                                                                                                                                                                        ggTGCTTCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACAGGCTTCAGTGCTGGTCCGTTCATTGCTGAAAAGTGAACGTCCTTCCGGACTGACTCAGGCAATCATTGAAGTggg                                              >  2:168502/1‑224 (MQ=255)

                                                                                                                                                                                                             ttCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACAGGCTTCAGTGCTGGTCCGTTCATTGCTGAAAAGTGAACGTCCTTCCGGACTGACTCAGGCAATCATTGAAGTGGGGCGCATCAACAAAACGCTGt                          >  1:804566/1‑239 (MQ=255)

                                                                                                                                                                                                              tCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACAGGCTTCAGTGCTGGTCCGTTCATTGCTGAAAAGTGAACGTCCTTCCGGACTGACTCAGGCAATCATTGAAGTGGGGCGCATCAACAAAACGCTGTAt                        <  1:75312/240‑1 (MQ=255)

                                                                                                                                                                                                              tCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACAGGCTTCAGTGCTGGTCCGTTCATTGCTGAAAAGTGAACGTCCTTCCGGACTGACTCAGGCAATCATTGAAGTGGGGCGCATCAACAAAACGCTGTAt                        <  1:458672/240‑1 (MQ=255)

                                                                                                                                                                                                              tCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACAGGCTTCAGTGCTGGTCCGTTCATTGCTGAAAAGTGAACGTCCTTCCGGACTGACTCAGGCAATCATTGAAGTGGGGCGCATCAACAAAACGCTGTAt                        <  1:410117/240‑1 (MQ=255)

                                                                                                                                                                                                              tCGGTTTTCTGGCGAATGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACAGGCTTCAGTGCTGGTCCGTTCATTGCTGAAAAGTGAACGTCCTTCCGGACTGACTCAGGCAATCATTGAAGTGGGGCGCATCAACAAAACGCTGTAt                        <  1:329327/240‑1 (MQ=255)

                                                                                                                                                                                                                             aTGGGCCATGATGCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACAGGCTTCAGTGCTGGTCCGTTCATTGCTGAAAAGTGAACGTCCTTCCGGACTGACTCAGGCAATCATTGAAGTGGGGCGCATCAACAAAACGCTGTATCTGCTTAATTATAtt         <  1:697340/240‑1 (MQ=255)

                                                                                                                                                                                                                                         gCCAACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATTAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACAGGCTTCAGTGCTGGTCCGTTCATTGCTGAAAAGTGAACGTCCTTCCGGACTGACTCAGGCAATCATTGAAGTGGGGCGCATCAACAAAACGCTGTATCTg                     <  1:591153/216‑1 (MQ=255)

CCGATCAGTATTCCGGCTTTCATGGCATCGTTATACCGGGGACGCTGAGGGACTCTATCTTTGTGCTGGAAGGCCTTCTGGAACAGGAGACCGGGCTGAATCCAACCGAAATTATGACCGATACAGCAGGTACCAGCGAACTTGTCTTTGGCCTTTTCTGGCTGCTGGGATACCAGTTTTCTCCACGCCTGGCTGATGCCGGTGCTTCGGTTTTCTGGCGAATGGACCATGATGCCGACTATGGCGTGCTGAATGATATTGCCAGAGGGCAATCAGATCCCCGAAAAATAGTCCTTCAGTGGGACGAAATGATCCGGACCGCTGGCTCCCTGAAACTGGGCAAAGTACAGGCTTCAGTGCTGGTCCGTTCATTGCTGAAAAGTGAACGTCCTTCCGGACTGACTCAGGCAATCATTGAAGTGGGGCGCATCAACAAAACGCTGTATCTGCTTAATTATATTGATGATG  >  BN000925/9566‑10033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`