Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,249,587	A→T	F11Y (TTT→TAT)	yeiE ←	putative LysR‑type transcriptional regulator YeiE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,249,587	0	A	T	100.0%	260.4 / NA	65	F11Y (TTT→TAT)	yeiE	putative LysR‑type transcriptional regulator YeiE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (24/41);  total (24/41)

CGCCGTTGTCTTCGCGAAACAGTTGTTCGATTTCAACCGCCTGTTCAAGCAATGCCAACGCACGCGGATAGAGCAGCCGCCCGTGTTCATTAACAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAATATCACCTTACAGAAACAACATCGTACACTGTGGCATTTTATTCCGGGGTTAGCCCTGAGTGCAGTTATCACCGGGGTCGCCCTGTG  >  NC_000913/2249351‑2249811                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                 cgcCGTTGTCTTCGCGAAACAGTTGTTCGATTTCAACCGCCTGTTCAAGCAATGCCAACGCACGCGGATAGAGCAGCCGCCCGTGTTCATTAACAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCataaa                                                                                                                                                                                                                               >  1:321657/1‑240 (MQ=255)      ttGTCTTCGCGAAACAGTTGTTCGATTTCAACCGCCTGTTCAAGCAATGCCAACGCGCGCGGATAGAGCAGCCGCCCGTGTTCATTAACAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCATTACTTCTGCATAAACTTcc                                                                                                                                                                                                                          <  1:260133/240‑1 (MQ=255)                      gttgttCGATTTCAACCGCCTGTTCAAGCAATGCCAACGCACGCGGATAGAGCAGCCGCCCGTGTTCATTAACAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTg                                                                                                                                                                                                           <  2:656606/239‑1 (MQ=255)                       ttgttCGATTTCAACCGCCTGTTCAAGCAATGCCAACGCACGCGGATAGAGCAGCCGCCCGTGTTCATTAACAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTg                                                                                                                                                                                                           <  2:193281/238‑1 (MQ=255)                                               aaGCAATGCCAACGCACGCGGATAGAGCAGCCGCCCGTGTTCATTAACAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTtata                                                                                                                                                                                 <  1:331096/240‑1 (MQ=255)                                                 gCAATGCCAACGCACGCGGATAGAGCAGCCGCCCGTGTTCATTAACAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATaaa                                                                                                                                                                               >  2:209840/1‑240 (MQ=255)                                                      gCCAACGCACGCGGATAGAGCAGCCGCCCGTGTTCATTAACAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGt                                                                                                                                                                                                            <  2:834761/206‑1 (MQ=255)                                                      gCCAACGCACGCGGATAGAGCAGCCGCCCGTGTTCATTAACAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGt                                                                                                                                                                                                            >  1:834765/1‑206 (MQ=255)                                                      gCCAACGCACGCGGATAGAGCAGCCGCCCGTGTTCATTAACAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGatta                                                                                                                                                                          >  1:67632/1‑240 (MQ=255)                                                               cgcgGATAGAGCAGCCGCCCGTGTTCATTAACAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATaaa                                                                                                                                                                  >  2:556967/1‑239 (MQ=255)                                                                          cagcCGCCCGTGTTCATTAACACCCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAg                                                                                                                                                                                                 <  1:784842/197‑1 (MQ=255)                                                                          cagcCGCCCGTGTTCATTAACAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTg                                                                                                                                                                                                           <  2:538121/187‑1 (MQ=255)                                                                          cagcCGCCCGTGTTCATTAACAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAat                                                                                                                                                      >  1:478791/1‑240 (MQ=255)                                                                            gccgccCGTGTTCATTAACAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGc                                                                                                                                                                                             >  1:484545/1‑199 (MQ=255)                                                                             ccgccCGTGTTCATTAACAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAatttt                                                                                                                                                   <  2:244237/240‑1 (MQ=255)                                                                             ccgccCGTGTTCATTAACAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAatttt                                                                                                                                                   >  1:469984/1‑240 (MQ=255)                                                                                      ttCATTAACAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTaaa                                                                                                                                          <  1:617880/240‑1 (MQ=255)                                                                                           tAACAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGt                                                                                                                                     <  2:67631/240‑1 (MQ=255)                                                                                             acaacCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGtt                                                                                                                                    <  2:169445/239‑1 (MQ=255)                                                                                                 ccAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCg                                                                                                                               <  1:766883/240‑1 (MQ=255)                                                                                                    gTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTa                                                                                                                             <  1:516681/239‑1 (MQ=255)                                                                                                             ccACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGatta                                                                                                                                                                          <  1:150286/185‑1 (MQ=255)                                                                                                              cACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGccc                                                                                                                  <  2:830026/240‑1 (MQ=255)                                                                                                              cACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGccc                                                                                                                  <  1:851226/240‑1 (MQ=255)                                                                                                                        aaCAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGga                                                                                                        <  1:195070/240‑1 (MQ=255)                                                                                                                               gCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGtt                                                                                                 >  1:503274/1‑240 (MQ=255)                                                                                                                                       aGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGaa                                                                                         >  1:706670/1‑240 (MQ=255)                                                                                                                                           gCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAatat                                                                                      <  2:552207/239‑1 (MQ=255)                                                                                                                                                  cAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAATATCACCTTAc                                                                              <  1:69912/240‑1 (MQ=255)                                                                                                                                                      tcggtcAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAATATCACCTTACAGaa                                                                          >  1:51420/1‑240 (MQ=255)                                                                                                                                                        ggtcAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAATATCACCTTACAGaaa                                                                         >  2:555556/1‑239 (MQ=255)                                                                                                                                                         gtcAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCATTAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAATATCACCTTACAGAaaca                                                                       <  1:94906/240‑1 (MQ=255)                                                                                                                                                         gtcAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAata                                                                                       <  2:2447/224‑1 (MQ=255)                                                                                                                                                         gtcAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAATATCACCTTACAGAaaca                                                                       <  2:387679/240‑1 (MQ=255)                                                                                                                                                         gtcAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAATATCACCTTACAGAaaca                                                                       <  1:729231/240‑1 (MQ=255)                                                                                                                                                         gtcAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAATATCACCTTACAGAaaca                                                                       <  2:294228/240‑1 (MQ=255)                                                                                                                                                         gtcAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTAAGACGAATATCACCTTACAGAaaca                                                                       >  2:372384/1‑240 (MQ=255)                                                                                                                                                          tcAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTACAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGt                                                                                                               >  1:647108/1‑199 (MQ=255)                                                                                                                                                           cAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAATATCACCTTACAGAaaca                                                                       <  1:324227/238‑1 (MQ=255)                                                                                                                                                                cTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTCTAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGga                                                                                                        <  2:579121/200‑1 (MQ=255)                                                                                                                                                                   cgcTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAATATCACCTTACAGAaacaac                                                                     >  2:572825/1‑232 (MQ=255)                                                                                                                                                                         ctgctgATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAATATCACCTTACAGAAACAACATCGTACACTGTgg                                                       >  2:463728/1‑240 (MQ=255)                                                                                                                                                                            ctgATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAATATCACCTTACAGAAACAACATCGTACACTGTGGCa                                                     <  2:122677/239‑1 (MQ=255)                                                                                                                                                                                  gCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAATATCACCTTACAGAaaca                                                                       <  2:821603/215‑1 (MQ=255)                                                                                                                                                                                         cGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAATATCACCTTACAGAAACAACATCGTACACTGTGGCATTTTATTcc                                            <  1:246820/235‑1 (MQ=255)                                                                                                                                                                                         cGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAATATCACCTTACAGAAACAACATCGTACACTGTGGCATTTTATTCCgggg                                        >  2:273496/1‑239 (MQ=255)                                                                                                                                                                                           ccAGCATCACCGACGCCTTGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAATATCACCTTACAGAAACAACATCGTACACtgtg                                                        <  2:419195/221‑1 (MQ=255)                                                                                                                                                                                            cAGCATCACCGACGACTGGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAATATCACCTTACAGAAACAACATCGTACACTGTGGCATTTTATTCCGGGGTTa                                     <  2:682887/239‑1 (MQ=255)                                                                                                                                                                                                 tCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAATATCACCTTACAGAAACAACATCGTACACTGTGGCATTTTATTCCGGGGTTAGCCCt                                <  1:538773/239‑1 (MQ=255)                                                                                                                                                                                                     ctaCGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAatat                                                                                      <  2:552135/179‑1 (MQ=255)                                                                                                                                                                                                         gCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAATATCACCTTACAGAAACAACATCGTACACTGTGGCATTTTATTCCGGGGTTAGCCCTGAGTGCAGt                       <  1:832731/240‑1 (MQ=255)                                                                                                                                                                                                              ggtggtTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCg                                                                                                                                                                                                                  >  2:569737/1‑48 (MQ=255)                                                                                                                                                                                                              ggtggtTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAATATCACCTTACAGAAACAACATCGTACACTGTGGCATTTTATTCCGGGGTTAGCCCTGAGTGCAGTTATCa                  >  1:171277/1‑240 (MQ=255)                                                                                                                                                                                                              ggtggtTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAATATCACCTTACAGAAACAACATCGTACACTGTGGCATTTTATTCCGGGGTTAGCCCTGAGTGCAGTTATCa                  >  1:145028/1‑240 (MQ=255)                                                                                                                                                                                                              ggtggtTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAATATCACCTTACAGAAACAACATCGTACACTGTGGCATTTTATTCCGGGGTTAGCCCTGAGTGCAGGTAt                    >  2:508517/1‑238 (MQ=255)                                                                                                                                                                                                               gtggtTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAATATCACCTTACAGAAACAACATCGTACACTGTGGCATTTTATTCCGGGGTTAGCCCTGAGTGCAGTTATCAc                 <  2:171275/240‑1 (MQ=255)                                                                                                                                                                                                               gtggtTGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAATATCACCTTACAGAAACAACATCGTACACTGTGGCATTTTATTCCGGGGTTAGCCCTGAGTGCAGTTATCAc                 <  1:68480/240‑1 (MQ=255)                                                                                                                                                                                                                    tGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAATATCACCTTACAGAAACAACATCGTACACTGTGGCATTTTATTCCGGGGTTAGCCCTGAGTGCAGTTATCACCgggg            <  2:345951/240‑1 (MQ=255)                                                                                                                                                                                                                    tGATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAATATCACCTTACAGAAACAACATCGTACACTGTGGCATTTTATTCCGGGGTTAGCCCTGAGTGCAGTTATCACCgggg            <  1:649235/240‑1 (MQ=255)                                                                                                                                                                                                                     gATCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGACTATCACCTTACAGAAACAACATCGTACACTGTGGCATTTTATTCCGGGGTTAGCCCTGAGTGCAGTTATCACCgggg            <  2:321655/239‑1 (MQ=255)                                                                                                                                                                                                                       tCCACTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAATATCACCTTACAGAAACAACATCGTACACTGTGGCATTTTATTCCGGGGTTAGCCCTGAGTGCAGTTATCACCGGGGTCg         <  1:184926/240‑1 (MQ=255)                                                                                                                                                                                                                          aCTTTTCAATACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCg                                                                                                                 >  2:266869/1‑133 (MQ=255)                                                                                                                                                                                                                             tttCAATACTTCTGCATAAACTTCCAACTGCCGGAg                                                                                                                                                                                                               >  2:241841/1‑36 (MQ=255)                                                                                                                                                                                                                                  aTACTTCTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATTTAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAATATCACCTTACAGAAACAACATCGTACACTGTGGCATTTTATTCCGGGGTTAGCCCTGAGTGCAGTTATCACCgggg            <  2:418396/226‑1 (MQ=255)                                                                                                                                                                                                                                        cTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAATATCACCTTACAGAAACAACATCGTACACTGTGGCATTTTATTCCGGGGTTAGCCCTGAGTGCAGTTATCACCGGGGTCGCCCtgtg  >  2:425526/1‑230 (MQ=255)                                                                                                                                                                                                                                        cTGCATAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTAGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAATATCACCTTACAGAaacaata                                                                    >  1:137286/1‑162 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                 CGCCGTTGTCTTCGCGAAACAGTTGTTCGATTTCAACCGCCTGTTCAAGCAATGCCAACGCACGCGGATAGAGCAGCCGCCCGTGTTCATTAACAACCAGTCTTTTCCCCACGCGATCAAACAGTTGCACGCCAAGCTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAATATCACCTTACAGAAACAACATCGTACACTGTGGCATTTTATTCCGGGGTTAGCCCTGAGTGCAGTTATCACCGGGGTCGCCCTGTG  >  NC_000913/2249351‑2249811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑